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Article ; Online: Comparative Study on Physical Characterization of Reclaimed Asphalt Pavement and Natural Aggregate in Flexible Pavement

Nandal Mohit / Sood Hemant / Gupta Pardeep Kumar

E3S Web of Conferences, Vol 405, p

2023 Volume 04041

Abstract: Reclaimed Asphalt Pavement (RAP) has gained significant attention in recent years as a sustainable alternative to natural aggregate in road construction. This study presents a comparison of the physical attributes of RAP and natural aggregate, with a ... ...

Abstract	Reclaimed Asphalt Pavement (RAP) has gained significant attention in recent years as a sustainable alternative to natural aggregate in road construction. This study presents a comparison of the physical attributes of RAP and natural aggregate, with a focus on their potential use as base and subbase materials in flexible pavements. The physical attributes of RAP and natural aggregate were evaluated using standard laboratory tests, including particle size distribution, specific gravity, water absorption, impact value test, Los Angles Abrasion test, flakiness and elongation test. The research concluded that RAP is comparable to natural aggregate in terms of particle size distribution and bulk density but has lower water absorption, impact, and abrasion values. The results shows that RAP is almost 53% more tougher that natural aggregate when tested for Aggregated Impact test and 30% more harder than natural aggregate when compared by Los Angeles abrasion test. Also, heatmap was used to analyse the relationship between physical attributes of natural aggregate and reclaimed asphalt pavement which depicted 93% similarity The findings suggest that RAP has the potential to replace natural aggregate in flexible pavements, further study is required to determine its long-term performance and durability.
Keywords	reclaimed asphalt pavement ; natural aggregate ; physical property tests ; sustainability ; flexible pavement ; Environmental sciences ; GE1-350
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Optimizing nutrient media conditions for continuous production of shoot biomass enriched in major medicinal constituents, amarogentin and mangiferin of endangered medicinal herb,

Gupta, Rolika / Sood, Hemant

Vegetos (Bareilly, India)

2022 , Page(s) 1–9

Abstract: Biosynthesis and accumulation of Amarogentin and Mangiferin from shoot culture of endangered ... ...

Abstract	Biosynthesis and accumulation of Amarogentin and Mangiferin from shoot culture of endangered herb
Language	English
Publishing date	2022-08-29
Publishing country	India
Document type	Journal Article
ZDB-ID	2726945-0
ISSN	2229-4473 ; 0970-4078
ISSN (online)	2229-4473
ISSN	0970-4078
DOI	10.1007/s42535-022-00464-6
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Article ; Online: ABC transporters mined through comparative transcriptomics associate with organ-specific accumulation of picrosides in a medicinal herb, Picrorhiza kurroa

Pandey, Roma / Sharma, Ashish / Sood, Hemant / Chauhan, Rajinder Singh

Protoplasma. 2023 Mar., v. 260, no. 2 p.453-466

2023

Abstract: Picrorhiza kurroa Royle ex Benth is a valuable medicinal herb of North-Western Himalayas due to presence of two major bioactive compounds, picroside-I and picroside-II used in the preparation of several hepatoprotective herbal drugs. These compounds ... ...

Abstract	Picrorhiza kurroa Royle ex Benth is a valuable medicinal herb of North-Western Himalayas due to presence of two major bioactive compounds, picroside-I and picroside-II used in the preparation of several hepatoprotective herbal drugs. These compounds accumulate in stolons/rhizomes; however, biosynthesized in different organs, viz., picroside-I in shoots and picroside-II in roots. As of today, no information exists on what transporters are transporting these metabolites from shoots and roots to the final storage organ, stolon, which ultimately transforms into rhizome. The ATP-binding cassette (ABC) transporters are reported to transport majority of secondary metabolites, including terpenoids in plants, therefore, we mined P. kurroa transcriptomes to identify and shortlist potential candidates. A total of 99 ABC transporter-encoding transcripts were identified in 3 differential transcriptomes, PKSS (shoots), PKSTS (stolons), and PKSR (roots) of P. kurroa, based on in silico comparative analysis and transcript abundance. 15 of these transcripts were further validated for their association using qRT-PCR in shoots, roots and stolon tissues in P. kurroa accessions varying for picroside-I and picroside-II contents. Organ-specific expression analysis revealed that PkABCA1, PkABCG1, and PkABCB5 had comparatively elevated expression in shoots; PkABCB2 and PkABCC2 in roots; PkABCB3 and PkABCC1 in stolon tissues of P. kurroa. Co-expression network analysis using ABC genes as hubs further unravelled important interactions with additional components of biosynthetic machinery. Our study has provided leads, first to our knowledge as of today, on putative ABC transporters possibly involved in long distance and local transport of picrosides in P. kurroa organs, thus opening avenues for designing a suitable genetic intervention strategy.
Keywords	Picrorhiza kurrooa ; biosynthesis ; computer simulation ; medicinal plants ; rhizomes ; secondary metabolites ; stolons ; terpenoids ; transcriptome ; transcriptomics ; Himalayan region
Language	English
Dates of publication	2023-03
Size	p. 453-466.
Publishing place	Springer Vienna
Document type	Article ; Online
ZDB-ID	123809-7
ISSN	1615-6102 ; 0033-183X
ISSN (online)	1615-6102
ISSN	0033-183X
DOI	10.1007/s00709-022-01786-7
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Article ; Online: Green synthesis of silver nanoparticles using Rhodiola imbricata and Withania somnifera root extract and their potential catalytic, antioxidant, cytotoxic and growth-promoting activities.

Kapoor, Sahil / Sood, Hemant / Saxena, Shweta / Chaurasia, Om Prakash

Bioprocess and biosystems engineering

2022 Volume 45, Issue 2, Page(s) 365–380

Abstract: This study presents the development of a sustainable production process of environmentally benign silver nanoparticles (AgNPs) from aqueous root extract of Rhodiola imbricata (RI) and Withania somnifera (WS) for mitigating environmental pollution and ... ...

Abstract	This study presents the development of a sustainable production process of environmentally benign silver nanoparticles (AgNPs) from aqueous root extract of Rhodiola imbricata (RI) and Withania somnifera (WS) for mitigating environmental pollution and investigating their potential applications in agriculture and biomedical industry. RIWS-AgNPs were characterized using several analytical techniques (UV-Vis, DLS, HR-TEM, SAED, EDX and FTIR). The antioxidant and anticancer activity of RIWS-AgNPs were estimated by DPPH and MTT assay, respectively. UV-Vis and DLS analysis indicated that equal ratio of RIWS-extract and silver nitrate (1:1) is optimum for green synthesis of well-dispersed AgNPs (λ
MeSH term(s)	Antineoplastic Agents/chemistry ; Antioxidants/chemistry ; Chlorophyll A ; Green Chemistry Technology ; Metal Nanoparticles/chemistry ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Rhodiola ; Silver/pharmacology ; Withania
Chemical Substances	Antineoplastic Agents ; Antioxidants ; Plant Extracts ; Silver (3M4G523W1G) ; Chlorophyll A (YF5Q9EJC8Y)
Language	English
Publishing date	2022-01-06
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1476357-6
ISSN	1615-7605 ; 1432-0797 ; 1615-7591
ISSN (online)	1615-7605 ; 1432-0797
ISSN	1615-7591
DOI	10.1007/s00449-021-02666-9
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Article ; Online: ABC transporters mined through comparative transcriptomics associate with organ-specific accumulation of picrosides in a medicinal herb, Picrorhiza kurroa.

Pandey, Roma / Sharma, Ashish / Sood, Hemant / Chauhan, Rajinder Singh

Protoplasma

2022 Volume 260, Issue 2, Page(s) 453–466

Abstract	Picrorhiza kurroa Royle ex Benth is a valuable medicinal herb of North-Western Himalayas due to presence of two major bioactive compounds, picroside-I and picroside-II used in the preparation of several hepatoprotective herbal drugs. These compounds accumulate in stolons/rhizomes; however, biosynthesized in different organs, viz., picroside-I in shoots and picroside-II in roots. As of today, no information exists on what transporters are transporting these metabolites from shoots and roots to the final storage organ, stolon, which ultimately transforms into rhizome. The ATP-binding cassette (ABC) transporters are reported to transport majority of secondary metabolites, including terpenoids in plants, therefore, we mined P. kurroa transcriptomes to identify and shortlist potential candidates. A total of 99 ABC transporter-encoding transcripts were identified in 3 differential transcriptomes, PKSS (shoots), PKSTS (stolons), and PKSR (roots) of P. kurroa, based on in silico comparative analysis and transcript abundance. 15 of these transcripts were further validated for their association using qRT-PCR in shoots, roots and stolon tissues in P. kurroa accessions varying for picroside-I and picroside-II contents. Organ-specific expression analysis revealed that PkABCA1, PkABCG1, and PkABCB5 had comparatively elevated expression in shoots; PkABCB2 and PkABCC2 in roots; PkABCB3 and PkABCC1 in stolon tissues of P. kurroa. Co-expression network analysis using ABC genes as hubs further unravelled important interactions with additional components of biosynthetic machinery. Our study has provided leads, first to our knowledge as of today, on putative ABC transporters possibly involved in long distance and local transport of picrosides in P. kurroa organs, thus opening avenues for designing a suitable genetic intervention strategy.
MeSH term(s)	Plants, Medicinal/genetics ; Plants, Medicinal/metabolism ; Transcriptome/genetics ; Picrorhiza/genetics ; Picrorhiza/chemistry ; Picrorhiza/metabolism ; ATP-Binding Cassette Transporters/genetics ; ATP-Binding Cassette Transporters/metabolism ; Gene Expression Profiling
Chemical Substances	ATP-Binding Cassette Transporters ; picroside II (39012-20-9) ; picroside I (27409-30-9)
Language	English
Publishing date	2022-06-29
Publishing country	Austria
Document type	Journal Article
ZDB-ID	123809-7
ISSN	1615-6102 ; 0033-183X
ISSN (online)	1615-6102
ISSN	0033-183X
DOI	10.1007/s00709-022-01786-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Circular Economy Enabler

Haq Md. Zia ul / Sood Hemant / Kumar Rajesh / Dixit Saurav / Srinivas T. / Ahuja Suniana / Lingam B. / Kumar Kaushal

E3S Web of Conferences, Vol 430, p

Enhancing High-Performance Bricks through Geopolymerization of Plastic Waste

2023 Volume 01202

Abstract: This article investigates the merging of geopolymerization and plastic waste usage, imagining high-performance brick production that couples innovation with sustainability, in an effort to transform the environmental effect of the building sector. This ... ...

Abstract	This article investigates the merging of geopolymerization and plastic waste usage, imagining high-performance brick production that couples innovation with sustainability, in an effort to transform the environmental effect of the building sector. This idea is supported by the circular economy, which diverts resources from waste streams into a closed-loop paradigm. By creating inorganic polymers from aluminosilicate-rich sources, the chemical process of geopolymerization provides a paradigm change in the production of materials. This procedure is improved even more by the addition of plastic trash, which combats plastic pollution and improves brick qualities. In order to create a more resilient and environmentally conscientious construction industry in the future, this paper outlines the process’s complexities, advantages, and difficulties while arguing for a harmonic fusion of circular economy concepts, technical innovation, and environmental stewardship.
Keywords	circular economy ; geopolymerization ; plastic waste ; high-performance bricks ; sustainability ; Environmental sciences ; GE1-350
Subject code	690
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Comparative coexpression networks pinpoint acyltransferases decorating structures of major iridoid glycosides in a medicinal herb, Picrorhiza kurroa

Sharma, Ashish / Sharma, Shilpa / Sood, Hemant / Chauhan, Rajinder Singh

Plant gene. 2022 Sept., v. 31

2022

Abstract: Picrorhiza kurroa, a hepatoprotective Himalayan medicinal herb contains a variety of iridoid glycosides mainly present in stolons and roots, contributing to several medicinal properties. However, off late the herb has been declared as endangered, ... ...

Abstract	Picrorhiza kurroa, a hepatoprotective Himalayan medicinal herb contains a variety of iridoid glycosides mainly present in stolons and roots, contributing to several medicinal properties. However, off late the herb has been declared as endangered, therefore, warranting alternate production routes for those iridoid glycosides. Catalpol, the major intermediary compound, is transformed into several iridoid glycosides through acylation by acyltransferase enzymes. In current study, we performed comparative gene co-expression-networks analysis among transcriptomes derived from different tissues/organs of P. kurroa varying for contents of iridoid glycosides to pinpoint major hubs associated with BAHD class of acyltransferases. Our analysis also captured other components co-expressed with major Acyltransferases hubs, which provided us leads as novel edges possibly contributing to other components of biosynthetic machinery. Some of the key interacting components such as Phytyl ester synthase (PES1 and PES2), callose synthase, serine carboxy peptidase like protein, polyphenol oxidase and UDP-glycosyltransferase were identified, which are possibly contributing to variations in the contents of iridoid glycosides in different tissues/organs of P. kurroa. Furthermore, the functional association of identified BAHD-ATs (SS_3469, STS_4084, STS_4241, STS_8424, SR_4494, and SR_4510) was evaluated through molecular docking to pinpoint probable BAHD-ATs transforming structural modifications of iridoid glycosides.
Keywords	1,3-beta-glucan synthase ; Picrorhiza kurrooa ; acylation ; acyltransferases ; biosynthesis ; catalpol ; catechol oxidase ; genes ; medicinal plants ; serine ; stolons ; transcriptome
Language	English
Dates of publication	2022-09
Publishing place	Elsevier B.V.
Document type	Article
ISSN	2352-4073
DOI	10.1016/j.plgene.2022.100366
Database	NAL-Catalogue (AGRICOLA)

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Article: Green synthesis of silver nanoparticles using Rhodiola imbricata and Withania somnifera root extract and their potential catalytic, antioxidant, cytotoxic and growth-promoting activities

Kapoor, Sahil / Sood, Hemant / Saxena, Shweta / Chaurasia, Om Prakash

Bioprocess and biosystems engineering. 2022 Feb., v. 45, no. 2

2022

Abstract	This study presents the development of a sustainable production process of environmentally benign silver nanoparticles (AgNPs) from aqueous root extract of Rhodiola imbricata (RI) and Withania somnifera (WS) for mitigating environmental pollution and investigating their potential applications in agriculture and biomedical industry. RIWS-AgNPs were characterized using several analytical techniques (UV–Vis, DLS, HR-TEM, SAED, EDX and FTIR). The antioxidant and anticancer activity of RIWS-AgNPs were estimated by DPPH and MTT assay, respectively. UV–Vis and DLS analysis indicated that equal ratio of RIWS-extract and silver nitrate (1:1) is optimum for green synthesis of well-dispersed AgNPs (λₘₐₓ: 430 nm, polydispersity index: 0.179, zeta potential: − 17.9 ± 4.14). HR-TEM and SAED analysis confirmed the formation of spherical and crystalline RIWS-AgNPs (37–42 nm). FTIR analysis demonstrated that the phenolic compounds are probably involved in stabilization of RIWS-AgNPs. RIWS-AgNPs showed effective catalytic degradation of hazardous environmental pollutant (4-nitrophenol). RIWS-AgNPs treatment significantly increased the growth and photosynthetic pigments of Hordeum vulgare in a size- and dose-dependent manner (germination (77%), chlorophyll a (12.62 ± 0.07 μg/ml) and total carotenoids (7.05 ± 0.04 μg/ml)). The DPPH assay demonstrated that RIWS-AgNPs exert concentration-dependent potent antioxidant activity (IC₅₀: 12.30 μg/ml, EC₅₀: 0.104 mg/ml, ARP: 959.45). Moreover, RIWS-AgNPs also confer strong cytotoxic activity against HepG2 cancer cell line in dose-dependent manner (cell viability: 9.51 ± 1.55%). Overall, the present study for the first time demonstrated a green technology for the synthesis of stable RIWS-AgNPs and their potential applications in biomedical and agriculture industry as phytostimulatory, antioxidant and anticancer agent. Moreover, RIWS-AgNPs could potentially be used as a green alternative for environmental remediation.
Keywords	Hordeum vulgare ; Rhodiola ; Withania somnifera ; agricultural industry ; antineoplastic activity ; antineoplastic agents ; antioxidant activity ; antioxidants ; bioprocessing ; carotenoids ; cell lines ; cell viability ; chlorophyll ; cytotoxicity ; dose response ; germination ; nanosilver ; neoplasm cells ; p-nitrophenol ; photosynthesis ; pollutants ; pollution ; remediation ; root extracts ; silver nitrate ; sustainable technology ; zeta potential
Language	English
Dates of publication	2022-02
Size	p. 365-380.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1476357-6
ISSN	1432-0797 ; 1615-7605 ; 1615-7591
ISSN (online)	1432-0797 ; 1615-7605
ISSN	1615-7591
DOI	10.1007/s00449-021-02666-9
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Article: Performance Evaluation of Pavement Geomaterials Stabilized with Pond Ash and Brick Kiln Dust Using Advanced Cyclic Triaxial Testing.

Gupta, Gaurav / Sood, Hemant / Gupta, Pardeep

Materials (Basel, Switzerland)

2020 Volume 13, Issue 3

Abstract: An increase in the generation of industrial waste materials such as pond ash and brick kiln dust represents a serious threat to the earth's environment. The waste materials have novel characteristics and therefore their physical, chemical, mineralogical, ...

Abstract	An increase in the generation of industrial waste materials such as pond ash and brick kiln dust represents a serious threat to the earth's environment. The waste materials have novel characteristics and therefore their physical, chemical, mineralogical, morphological, mechanical, and toxicity characteristics were determined so that these materials can be incorporated as construction materials based on technical and environmental considerations. The purpose of this study is to utilize the wastes in the stabilization of clay geomaterial to outperform existing problems of inadequate strength and stiffness of the subgrade layer in flexible pavements. Mixtures of geomaterials, containing pond ash, brick kiln dust, and their combination were prepared and important engineering characteristics such as the plasticity, compaction, and strength of the mixtures were examined. The measurement of response to dynamic loading is a pre-condition for the accurate characterization of geomaterials used in pavement construction. Using advanced cyclic triaxial testing, this study evaluates the performance of pond ash and brick kiln dust in increasing the stiffness of the geomaterial under cyclic loading. To stimulate the worst field conditions, the stiffness and strength tests were performed under standard and four-day water-soaked conditions. The implementation of several stress-dependent models for the prediction of stiffness was examined. Pond ash and brick kiln dust were found to be effective in increasing the stiffness and strength of the geomaterial. The wastes were the most effectual when added in combination to the geomaterial. The characterization of the wastes was informative in understanding the governing mechanism prevalent in the waste stabilized mixtures. The toxicity characterization study revealed the non-toxic and non-hazardous nature of the waste materials, permitting their use in the construction of pavements. This study recommends the use of wastes in the subgrade of flexible pavements. Further research is needed for performance evaluation of the wastes on silt and sand geomaterials for their wider application.
Language	English
Publishing date	2020-01-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma13030553
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Eco-Friendly Building Material Innovation

Haq Md. Zia ul / Sood Hemant / Kumar Rajesh / Sharma Vishal / Kumar Anuj / Srinivas T. / Gulati Monica / Bindu K. Hima / Kumar Kaushal

E3S Web of Conferences, Vol 430, p

Geopolymer Bricks from Repurposed Plastic Waste

2023 Volume 01201

Abstract: This study compares the ecological footprints of geopolymer and red clay brick prisms, two common building materials for long-lasting masonry structures. The study’s goal is to shed light on the environmental performance of different brick kinds by a ... ...

Abstract	This study compares the ecological footprints of geopolymer and red clay brick prisms, two common building materials for long-lasting masonry structures. The study’s goal is to shed light on the environmental performance of different brick kinds by a thorough review of sustainability indices such as embodied energy, CO2 emissions, water use, and trash creation. The results suggest that geopolymer bricks have better environmental features than red clay bricks, such as lower embodied energy, decreased CO2 emissions, lower water consumption, and less waste creation. These findings underline the promise of geopolymer bricks as an eco-friendlier masonry alternative that may improve green building performance. The report, however, stresses the need to think about more than only environmental damage. The sustainability and feasibility of utilising geopolymer and red clay bricks depend heavily on factors including durability, thermal performance, and cost-effectiveness. In order to make educated selections about brick selection, it is important to evaluate these variables. The results of this study provide the groundwork for more research on sustainable masonry materials and contribute to the development of environmentally aware building practises. Architectural and engineering professionals may encourage environmentally responsible building practises and help create a more sustainable and resilient built environment by taking this study’s findings into account.
Keywords	geopolymer ; red clay brick ; sustainability ; environmental impact ; masonry construction ; Environmental sciences ; GE1-350
Subject code	690
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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