Article ; Online: L-glutamine supplementation reduced morphological damage in the renal glomerulus of rats with Walker-256 tumor.
2023 Volume 38, Page(s) e383923
Abstract: Purpose: To evaluate the effects of the experimental subcutaneous Walker-256 tumor and L-glutamine supplementation, an antioxidant, on the glomerular morphology of rats.: Methods: Twenty Wistar rats were distributed into four groups (n = 5): control ( ...
| Abstract | Purpose: To evaluate the effects of the experimental subcutaneous Walker-256 tumor and L-glutamine supplementation, an antioxidant, on the glomerular morphology of rats. Methods: Twenty Wistar rats were distributed into four groups (n = 5): control (C); control treated with 2% L-glutamine (CG); rats with Walker-256 tumor (WT); and rats with Walker-256 tumor treated with 2% L-glutamine (WTG). Renal histological samples were submitted to periodic acid-Schiff and Masson's Trichrome staining to analyze glomerular density, morphometry of glomerular components and glomerulosclerosis; and to immunohistochemistry for fibroblast growth factor-2 (FGF-2). Results: WT showed 50% reduction in body mass gain and cachexia index > 10%, while WTG demonstrated reduction in cachexia (p < 0.05). WT revealed reduction of glomerular density, increase in the glomerular tuft area, mesangial area, matrix in the glomerular tuft, decrease in the urinary space and synechia, and consequently higher glomerulosclerosis (p < 0.05). L-glutamine supplementation in the WTG improved glomerular density, and reduced glomerular tuft area, urinary space, mesangial area, and glomerulosclerosis compared to WT(p < 0.05). WT showed higher collagen area and FGF-2 expression compared to C (p < 0.05). WTG presented lower collagen fibers and FGF-2 expression compared to WT (p < 0.05). Conclusions: L-glutamine supplementation reduced cachexia and was beneficial for glomerular morphology of the rats, as well as it reduced kidney damage and improved the remaining glomeruli morphology. |
|---|---|
| MeSH term(s) | Rats ; Animals ; Rats, Wistar ; Glutamine/pharmacology ; Cachexia/metabolism ; Cachexia/pathology ; Fibroblast Growth Factor 2 ; Neoplasms ; Dietary Supplements ; Collagen |
| Chemical Substances | Glutamine (0RH81L854J) ; Fibroblast Growth Factor 2 (103107-01-3) ; Collagen (9007-34-5) |
| Language | English |
| Publishing date | 2023-10-13 |
| Publishing country | Brazil |
| Document type | Journal Article |
| ZDB-ID | 2012156-8 |
| ISSN | 1678-2674 ; 1678-2674 |
| ISSN (online) | 1678-2674 |
| ISSN | 1678-2674 |
| DOI | 10.1590/acb383923 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.