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Article: Caffeine but not acetaminophen increases 4-km cycling time-trial performance

Tomazini, F / Santos-Mariano, A.C / Andrade-Souza, V.A / Sebben, V.C / De Maria, C.A.B / Coelho, D.B / Bertuzzi, R / Silva-Cavalcante, M.D / Lima-Silva, A.E

PharmaNutrition. 2020 June, v. 12

2020

Abstract: Acetaminophen has been combined with caffeine for therapeutic purpose, but the effect of co-ingestion of acetaminophen and caffeine on exercise performance has not been investigated. To determine the effect of isolated and combined ingestion of caffeine ... ...

Abstract	Acetaminophen has been combined with caffeine for therapeutic purpose, but the effect of co-ingestion of acetaminophen and caffeine on exercise performance has not been investigated. To determine the effect of isolated and combined ingestion of caffeine and acetaminophen on performance during a 4-km cycling time-trial (4TT). Eleven men, completed a 4TT one hour after the ingestion of cellulose (PLA), acetaminophen (20 mg kg−1 body mass, ACT), caffeine (5 mg kg−1 body mass, CAF) or combined acetaminophen and caffeine (20 and 5 mg kg−1 body mass, respectively, ACTCAF). The perception of pain, rating of perceived exertion, electromyography, oxygen uptake were recorded. Plasma lactate concentration was measured before and immediately after the trial. The time and mean power during the 4TT was significantly improved (P < 0.05) in CAF (407.9 ± 24.5 s, 241.4 ± 16.1 W) compared to PLA (416.1 ± 34.1 s, 234.1 ± 19.2 W) and ACT (416.2 ± 26.6 s, 235.8 ± 19.7 W). However, there was no difference between ACTCAF (411.6 ± 27.7 s, 238.7 ± 18.7 W) and the other conditions (P > 0.05). All other measures were similar under the conditions (P > 0.05). Caffeine but not acetaminophen increases power output ultimately increasing performance during a 4TT.
Keywords	acetaminophen ; athletic performance ; caffeine ; cellulose ; electromyography ; lactic acid ; men ; oxygen ; pain ; therapeutics
Language	English
Dates of publication	2020-06
Publishing place	Elsevier B.V.
Document type	Article
ISSN	2213-4344
DOI	10.1016/j.phanu.2020.100181
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Molecular cytogenetics of nucleolar organizer regions in Phyllomedusa and Phasmahyla species (Hylidae, Phyllomedusinae): a cytotaxonomic contribution.

Barth, A / Souza, V A / Solé, M / Costa, M A

Genetics and molecular research : GMR

2013 Volume 12, Issue 3, Page(s) 2400–2408

Abstract: Chromosome numbers, morphology, and nucleolus organizer region (NOR) locations are useful cytological characters for taxonomic and evolutionary studies. In this study, we provide the first cytogenetic analysis of Phyllomedusa bahiana and Phasmahyla ... ...

Abstract	Chromosome numbers, morphology, and nucleolus organizer region (NOR) locations are useful cytological characters for taxonomic and evolutionary studies. In this study, we provide the first cytogenetic analysis of Phyllomedusa bahiana and Phasmahyla spectabilis, and report new cytogenetic data on variation in NOR numbers and positions in Phyllomedusa rohdei and Phyllomedusa nordestina using conventional staining, AgNOR-banding, and 45S rDNA fluorescence in situ hybridization. All 4 species showed 2n = 26 chromosomes. P. spectabilis and P. bahiana had only 1 pair of NOR-carrying chromosomes. P. bahiana showed an NOR length polymorphism, and a rare homomorphic self-compatibility for both NOR lengths in anurans. Variation in the number of NOR-bearing chromosomes was found between the sampled populations of P. nordestina, ranging from 3 to 4. This study also clarified previous conflicting results concerning the occurrence of inter- and intra-population NOR variation in P. rohdei. The variation, confirmed by 45S rDNA fluorescence in situ hybridization analysis, was congruent with results obtained from AgNOR-banding in all species.
MeSH term(s)	Animals ; Anura/classification ; Anura/genetics ; In Situ Hybridization, Fluorescence ; Karyotype ; Nucleolus Organizer Region/genetics ; Phylogeny ; Polymorphism, Genetic ; Population/genetics
Language	English
Publishing date	2013-07-15
Publishing country	Brazil
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2114039-X
ISSN	1676-5680 ; 1676-5680
ISSN (online)	1676-5680
ISSN	1676-5680
DOI	10.4238/2013.July.15.3
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Article ; Online: Molecular characterization of Platonia insignis Mart. ("bacurizeiro") using inter simple sequence repeat (ISSR) markers.

Souza, I G B / Souza, V A B / Lima, P S C

Molecular biology reports

2012 Volume 40, Issue 5, Page(s) 3835–3845

Abstract: Platonia insignis Mart. (Clusiaceae) is widespread throughout the Amazon and adjacent areas. The fruits (known locally as "bacuri") have significant commercial potential, but the species is under threat from agro-industrial expansion. The genetic ... ...

Abstract	Platonia insignis Mart. (Clusiaceae) is widespread throughout the Amazon and adjacent areas. The fruits (known locally as "bacuri") have significant commercial potential, but the species is under threat from agro-industrial expansion. The genetic variability within 72 genotypes of P. insignis belonging to ten populations collected in the Brazilian states of Maranhão and Piauí, and maintained in the germplasm collection of Embrapa Meio-Norte, has been determined, and the organization of genetic diversity within populations, between populations and among geographic groups verified. Eighteen selected inter simple sequence repeat primers allowed amplification of 236 loci of which 221 (93.64%) were polymorphic, indicating a high level of genetic diversity. At the population level, the Shannon and Nei diversity indices ranged from 0.082 to 0.323 and from 0.120 to 0.480, respectively. The global coefficient of genetic differentiation (G(ST)) was 0.4730 indicating that differentiation between populations was significant, a finding that was confirmed by analysis of molecular variance (Φ(ST) = 0.28). UPGMA cluster analysis revealed that the genotypes could be stratified into groups that were well defined and consistent with those identified in the dendrogram constructed using pair wise Φ(ST) values. The high genetic diversity established in this study may facilitate the management and conservation of the germplasm of P. insignis.
MeSH term(s)	Brazil ; Cluster Analysis ; DNA, Plant ; Ferns/genetics ; Genetic Variation ; Genotype ; Geography ; Microsatellite Repeats
Chemical Substances	DNA, Plant
Language	English
Publishing date	2012-12-30
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	186544-4
ISSN	1573-4978 ; 0301-4851
ISSN (online)	1573-4978
ISSN	0301-4851
DOI	10.1007/s11033-012-2462-6
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Article ; Online: Molecular characterization of cajá, Spondias mombin (Anacardiaceae), by RAPD markers.

Lima, A T B / de Souza, V A B / Gomes, R L F / Lima, P S C

Genetics and molecular research : GMR

2011 Volume 10, Issue 4, Page(s) 2893–2904

Abstract: The arboreal species Spondias mombin L. (Anacardiaceae) is widely distributed in Brazil, where the fruits, known by the common name of cajá, are an important commercial commodity. We evaluated genetic variability among 32 cajá accessions of the ... ...

Abstract	The arboreal species Spondias mombin L. (Anacardiaceae) is widely distributed in Brazil, where the fruits, known by the common name of cajá, are an important commercial commodity. We evaluated genetic variability among 32 cajá accessions of the Germplasm Collection of Embrapa Meio-Norte using RAPD technique. Reaction conditions for efficient RAPD amplifications were optimized in preliminary tests, and primers were selected from a set designed by the University of British Columbia on the basis of high levels of polymorphism and adequate band resolution. The 21 primers employed in the final analysis produced 145 fragments, 79% of which were polymorphic. Based on the RAPD data, a dendrogram was constructed using the unweighted pair group method with arithmetic mean clustering technique. The 32 cajá accessions were classified into three main groups with a mean genetic similarity of 68.8%. Group I comprised 26 accessions (74.1% similarity), and group II included five accessions (74.0% similarity), while group III consisted of one accession (BGC 06), which exhibited the lowest similarity coefficients. Accessions BGC 06 and BGC 31 were the most unrelated and, hence, most suitable for initial crossings in order to obtain high levels of segregation. We concluded, based on the repeatability and reproducibility tests, that the RAPD technique is reliable and efficient for revealing the genetic diversity of cajá accessions, which will be useful for genetic improvement programs.
MeSH term(s)	Anacardiaceae/classification ; Anacardiaceae/genetics ; Brazil ; Breeding ; Cluster Analysis ; DNA Primers ; DNA, Plant/genetics ; Fruit/classification ; Fruit/genetics ; Genetic Markers ; Phylogeny ; Polymorphism, Genetic ; Random Amplified Polymorphic DNA Technique/methods ; Reproducibility of Results ; Species Specificity
Chemical Substances	DNA Primers ; DNA, Plant ; Genetic Markers
Language	English
Publishing date	2011-11-25
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2114039-X
ISSN	1676-5680 ; 1676-5680
ISSN (online)	1676-5680
ISSN	1676-5680
DOI	10.4238/2011.November.25.1
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Article ; Online: Medical education on fitness to drive: a survey of all UK medical schools.

Hawley, C A / Galbraith, N D / deSouza, V A

Postgraduate medical journal

2008 Volume 84, Issue 998, Page(s) 635–638

Abstract: Aim: To identify the extent to which medical aspects of fitness to drive (FTD) are taught within UK medical schools.: Methods: A survey of all 32 UK medical schools. In-depth interviews with a range of staff at two medical schools; telephone survey ... ...

Abstract	Aim: To identify the extent to which medical aspects of fitness to drive (FTD) are taught within UK medical schools. Methods: A survey of all 32 UK medical schools. In-depth interviews with a range of staff at two medical schools; telephone survey of 30 schools. Results: Two thirds of schools reported specific teaching on medical aspects of FTD but few covered it in any depth or in relation to specific medical conditions. Only one school taught FTD in relation to elderly medicine. FTD was an examination topic at only 12 schools. Conclusion: Teaching on FTD is inconsistent across UK medical schools. Many new doctors will graduate with limited knowledge of medical aspects of FTD.
MeSH term(s)	Automobile Driving ; Curriculum/statistics & numerical data ; Education, Medical, Undergraduate/statistics & numerical data ; Schools, Medical/statistics & numerical data ; Teaching/statistics & numerical data ; United Kingdom
Language	English
Publishing date	2008-12
Publishing country	England
Document type	Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
ZDB-ID	80325-x
ISSN	1469-0756 ; 0032-5473
ISSN (online)	1469-0756
ISSN	0032-5473
DOI	10.1136/pgmj.2008.067959
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Article ; Online: RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil.

Souza, I G B / Valente, S E S / Britto, F B / de Souza, V A B / Lima, P S C

Genetics and molecular research : GMR

2011 Volume 10, Issue 4, Page(s) 3080–3089

Abstract: We evaluated genetic variability of mango (Mangifera indica) accessions maintained in the Active Germplasm Bank of Embrapa Meio-Norte in Teresina, Piauí, Brazil, using RAPDs. Among these accessions, 35 originated from plantings in Brazil, six from ... ...

Abstract	We evaluated genetic variability of mango (Mangifera indica) accessions maintained in the Active Germplasm Bank of Embrapa Meio-Norte in Teresina, Piauí, Brazil, using RAPDs. Among these accessions, 35 originated from plantings in Brazil, six from the USA and one from India. Genomic DNA, extracted from leaf material using a commercial purification kit, was subjected to PCR with the primers A01, A09, G03, G10, N05, and M16. Fifty-five polymorphic loci were identified, with mean of 9.16 ± 3.31 bands per primer and 100% polymorphism. Application of unweighted pair group method using arithmetic average cluster analysis demonstrated five genotypic groups among the accessions examined. The genotypes Rosa 41, Rosa 48 and Rosa 49 were highly similar (94% similarity), whereas genotypes Sensation and Rosa 18 were the most divergent (only 7% similarity). The mango accessions were found to have considerable genetic variability, demonstrating the importance of analyzing each genotype in a collection in order to efficiently maintain the germplasm collection.
MeSH term(s)	Brazil ; Breeding ; Cluster Analysis ; DNA Primers/genetics ; DNA, Plant/genetics ; Databases, Genetic ; Genetic Markers ; Genotype ; Germ Cells, Plant/cytology ; Germ Cells, Plant/metabolism ; India ; Mangifera/genetics ; Phylogeny ; Plant Leaves/genetics ; Polymorphism, Genetic ; Random Amplified Polymorphic DNA Technique/methods ; United States
Chemical Substances	DNA Primers ; DNA, Plant ; Genetic Markers
Language	English
Publishing date	2011-12-14
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2114039-X
ISSN	1676-5680 ; 1676-5680
ISSN (online)	1676-5680
ISSN	1676-5680
DOI	10.4238/2011.December.14.1
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Article: Genetic variability in mango genotypes detected by RAPD markers

Souza, V.A.B. de / Lima, P.S. da C

Acta horticulturae. 2004 Feb., no. 645

2004

Keywords	Mangifera indica ; mangoes ; genotype ; random amplified polymorphic DNA technique ; genetic variation ; genetic markers
Language	English
Dates of publication	2004-02
Size	p. 303-310.
Document type	Article
Note	Paper presented at the Seventh International Mango Symposium, September 22-27, 2002, Recife City, Brazil.
ISSN	0567-7572
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Anti-tuberculosis neolignans from Piper regnellii.

Scodro, R B L / Pires, C T A / Carrara, V S / Lemos, C O T / Cardozo-Filho, L / Souza, V A / Corrêa, A G / Siqueira, V L D / Lonardoni, M V C / Cardoso, R F / Cortez, D A G

Phytomedicine : international journal of phytotherapy and phytopharmacology

2013 Volume 20, Issue 7, Page(s) 600–604

Abstract: The present study determined the anti-Mycobacterium tuberculosis activities of supercritical CO2 extracts, neolignans eupomatenoid-5 (1), conocarpan (4) and eupomatenoid-3 (7) and their derivatives (2, 3, 5, 6, and 8) from Piper regnellii, as well as ... ...

Abstract	The present study determined the anti-Mycobacterium tuberculosis activities of supercritical CO2 extracts, neolignans eupomatenoid-5 (1), conocarpan (4) and eupomatenoid-3 (7) and their derivatives (2, 3, 5, 6, and 8) from Piper regnellii, as well as their cytotoxicities. The supercritical CO2 extract from leaves was purified by chromatographic methods, yielding compounds (1), (4) and (7), which were identified by (1)H NMR and comparison with literature data. Anti-M. tuberculosis activity (H37Rv and clinical isolates) was evaluated using a resazurin microtiter assay plate (REMA) to determine the MIC. The cytotoxicity assay was carried out in macrophages J774G.8 by sulforhodamine B colorimetric assay. The supercritical CO2 extracts from leaves and stems, and compound (4) showed activity against M. tuberculosis (MIC 15.6 μg/ml). Compound (1) showed the best activity (MIC 1.9 μg/ml), with good SI. Compounds (7) and (8) showed low activity against M. tuberculosis H37Rv. The derivative compounds did not show increased anti-M. tuberculosis activity. This is the first report, to our knowledge, to describe neolignans from P. regnellii with activity against M. tuberculosis, and compound (1) is a potential candidate for future antituberculosis drugs.
MeSH term(s)	Animals ; Antitubercular Agents/chemistry ; Antitubercular Agents/pharmacology ; Benzofurans/chemistry ; Benzofurans/pharmacology ; Cell Line/drug effects ; Lignans/chemistry ; Lignans/pharmacology ; Macrophages/drug effects ; Magnetic Resonance Spectroscopy ; Mice ; Microbial Sensitivity Tests ; Molecular Structure ; Mycobacterium tuberculosis/drug effects ; Phenols/chemistry ; Phenols/pharmacology ; Piper/chemistry ; Plant Extracts/analysis ; Plant Extracts/chemistry ; Toxicity Tests/methods
Chemical Substances	Antitubercular Agents ; Benzofurans ; Lignans ; Phenols ; Plant Extracts ; eupomatenoid-3 ; conocarpan (30358-74-8) ; eupomatenoid 5 (41744-28-9)
Language	English
Publishing date	2013-05-15
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2013.01.005
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Article: Serological survey for leptospirosis in sheep flocks from Igarape-Açu, state of Para

Moraes, C.C.G. de / Guereiro, A. do N. / Kuroda, R.B. dos S. / Souza, V.A.F. de / Meneses, A.M.C. / Vasconcelos, S.A.

Revista de Ciencias Agrarias (Brazil)

(Jan-Mar 2012) Volume v. 55, Issue (1), Page(s) p. 58–60

Keywords	SHEEP ; LEPTOSPIROSIS ; BACTERIA ; ANTIBODIES ; OVIN ; LEPTOSPIROSE ; BACTERIA ; ANTICORPS ; OVINOS ; LEPTOSPIROSIS ; BACTERIA ; ANTICUERPOS ; http://www.fao.org/aos/agrovoc#c_7030 ; http://www.fao.org/aos/agrovoc#c_4281 ; http://www.fao.org/aos/agrovoc#c_765 ; http://www.fao.org/aos/agrovoc#c_493
Language	Portuguese
Document type	Article
ISSN	1517-591X
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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Article: Winery by-products: extraction optimization, phenolic composition and cytotoxic evaluation to act as a new source of scavenging of reactive oxygen species

Melo, P. S. / Massarioli, A. P. / Denny, C. / Santos, L. F. dos / Franchin, M. / Pereira, G. E. / Souza, V. a / Rosalen, P. L. / Alencar, S. M. de

Food Chemistry

2015 Volume 181, Page(s) 160–169

Abstract: winery by-products; anthocyanins; response surface methodology (RSM); reactive oxygen species (ROS); cytotoxicity (MTT). [33415] ...

Institution	Department of Agri-food Industry, Food and Nutrition, 'Luiz de Queiroz' College of Agriculture, University of Sao Paulo, Padua Dias Avenue, P.O. Box. 9, 13418-900 Piracicaba, SP, Brazil
Abstract	winery by-products; anthocyanins; response surface methodology (RSM); reactive oxygen species (ROS); cytotoxicity (MTT). [33415]
Language	English
Document type	Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2015.02.087
Database	Viticulture and Oenology Abstracts

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