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  1. Article ; Online: Novel, Synchronous, First-person Perspective Virtual Simulations for Medical Students in Emergency Medicine.

    Sperandeo, Michael / Moadel, Tiffany / Yun, Sezzy / Pollack, Stephanie / Cassara, Michael

    The western journal of emergency medicine

    2023  Volume 24, Issue 1, Page(s) 68–70

    MeSH term(s) Humans ; Students, Medical ; User-Computer Interface ; Emergency Medicine/education
    Language English
    Publishing date 2023-01-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2375700-0
    ISSN 1936-9018 ; 1936-9018
    ISSN (online) 1936-9018
    ISSN 1936-9018
    DOI 10.5811/westjem.2022.11.57764
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Acute Toxin-mediated Rhabdomyolysis During Treatment With Trimethoprim-sulfamethoxazole.

    Sperandeo, Michael / Pantic, Dorjan / Army, Jessica

    Clinical practice and cases in emergency medicine

    2019  Volume 3, Issue 4, Page(s) 357–360

    Abstract: Rhabdomyolysis is a condition in which skeletal muscle breakdown causes the release of intracellular components into the bloodstream - defined as elevations in serum creatine kinase levels. The etiology of rhabdomyolysis is varied and may be the result ... ...

    Abstract Rhabdomyolysis is a condition in which skeletal muscle breakdown causes the release of intracellular components into the bloodstream - defined as elevations in serum creatine kinase levels. The etiology of rhabdomyolysis is varied and may be the result of toxin-mediated mechanisms or metabolic derangements, or they may develop secondary to other conditions such as seizures, trauma and prolonged immobilization. In this case, we present a patient with suspected acute toxin-mediated rhabdomyolysis in the setting of trimethoprim-sulfamethoxazole (TMP-SMX) therapy for urinary tract infection. To our knowledge, this marks the fifth case report of an otherwise healthy patient diagnosed with rhabdomyolysis thought to be secondary to TMP-SMX.
    Language English
    Publishing date 2019-09-23
    Publishing country United States
    Document type Case Reports
    ISSN 2474-252X
    ISSN (online) 2474-252X
    DOI 10.5811/cpcem.2019.7.42688
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: High cell density attenuates reactive oxygen species: implications for in vitro assays.

    Kim, Dennis P / Yahav, Jonathan / Sperandeo, Michael / Maloney, Lauren / McTigue, Monica / Lin, Fubao / Clark, Richard A F

    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

    2012  Volume 20, Issue 1, Page(s) 74–82

    Abstract: In vitro cell-based assays are an essential and universally used step in elucidation of biological processes as well as in drug development. However, results obtained depend on the validity of protocols used. This statement certainly pertains to in vitro ...

    Abstract In vitro cell-based assays are an essential and universally used step in elucidation of biological processes as well as in drug development. However, results obtained depend on the validity of protocols used. This statement certainly pertains to in vitro assays of oxidative stress. The holy grail of in vitro models is reliability and predictability of outcomes that relate to a single variable like addition of hydrogen peroxide or xanthine oxidase. Without such validated outcomes, comparison of results among different laboratories is not possible. Achieving this goal requires a thorough understanding of the complex interplay between the cells, their environment, and the experimental assays. Furthermore, as this knowledge is attained, it must be disseminated and used to update and standardize existing protocols. Here, we confirm and extend the effect of pyruvate and cell density on in vitro oxidative stress assays. Cell viability was assessed using a colorimetric assay measuring the reduction of a tetrazolium salt (XTT) into a colored formazan dye. Extracellular hydrogen peroxide concentrations were measured using the foxp3 assay. We confirmed a previously reported finding that pyruvate, a common ingredient in cell culture media, acts as an extracellular scavenger of reactive oxygen species. We also demonstrated that cell density directly correlates with resistance to oxidative stress in tissue culture. It is theorized that the protective effect due to cell density predominantly relates to intracellular factors such as reduced glutathione and extracellular factors such as catalase.
    MeSH term(s) Cell Count ; Cell Survival/drug effects ; Cells, Cultured ; Colorimetry ; Extracellular Space/chemistry ; Female ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Fibroblasts/pathology ; Forkhead Transcription Factors/analysis ; Formazans/analysis ; Free Radical Scavengers/pharmacology ; Humans ; Hydrogen Peroxide/analysis ; Hydrogen Peroxide/metabolism ; Indicators and Reagents/analysis ; Oxidative Stress/drug effects ; Pyruvic Acid/pharmacology ; Reactive Oxygen Species/metabolism ; Reproducibility of Results ; Tetrazolium Salts/analysis ; Tetrazolium Salts/metabolism
    Chemical Substances FOXP3 protein, human ; Forkhead Transcription Factors ; Formazans ; Free Radical Scavengers ; Indicators and Reagents ; Reactive Oxygen Species ; Tetrazolium Salts ; Pyruvic Acid (8558G7RUTR) ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2012-01
    Publishing country United States
    Document type Journal Article ; Research Support, American Recovery and Reinvestment Act ; Research Support, N.I.H., Extramural
    ZDB-ID 1174873-4
    ISSN 1524-475X ; 1067-1927
    ISSN (online) 1524-475X
    ISSN 1067-1927
    DOI 10.1111/j.1524-475X.2011.00746.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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