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Article ; Online: Low Magnesium Concentration Enforces Bone Calcium Deposition Irrespective of 1,25-Dihydroxyvitamin D

Rashid, Usman / Becker, Sandra K / Sponder, Gerhard / Trappe, Susanne / Sandhu, Mansur A / Aschenbach, Jörg R

International journal of molecular sciences

2023 Volume 24, Issue 10

Abstract: Efficient coordination between ... ...

Abstract	Efficient coordination between Mg
MeSH term(s)	Female ; Animals ; Cattle ; Calcium/metabolism ; Magnesium/pharmacology ; Magnesium/metabolism ; Gene Expression Regulation ; Vitamin D3 24-Hydroxylase/genetics ; Vitamin D3 24-Hydroxylase/metabolism ; Vitamin D/metabolism ; RNA, Messenger ; Hydroxyapatites/metabolism
Chemical Substances	1,25-dihydroxyvitamin D (66772-14-3) ; Calcium (SY7Q814VUP) ; Magnesium (I38ZP9992A) ; Vitamin D3 24-Hydroxylase (EC 1.14.15.16) ; Vitamin D (1406-16-2) ; RNA, Messenger ; Hydroxyapatites
Language	English
Publishing date	2023-05-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24108679
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Article ; Online: Effects of butyrate

Liebe, Franziska / Liebe, Hendrik / Sponder, Gerhard / Mergler, Stefan / Stumpff, Friederike

Pflugers Archiv : European journal of physiology

2022 Volume 474, Issue 3, Page(s) 315–342

Abstract: The ruminal epithelium absorbs large quantities of ... ...

Abstract	The ruminal epithelium absorbs large quantities of NH
MeSH term(s)	Animals ; Biological Transport/physiology ; Butyrates/metabolism ; Cattle ; Epithelium/metabolism ; HEK293 Cells ; Humans ; Hydrogen-Ion Concentration ; TRPV Cation Channels/metabolism
Chemical Substances	Butyrates ; TRPV Cation Channels ; TRPV3 protein, human ; TRPV4 protein, human
Language	English
Publishing date	2022-01-31
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	6380-0
ISSN	1432-2013 ; 0031-6768
ISSN (online)	1432-2013
ISSN	0031-6768
DOI	10.1007/s00424-021-02647-7
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Article ; Online: Beyond Ca

Liebe, Hendrik / Liebe, Franziska / Sponder, Gerhard / Hedtrich, Sarah / Stumpff, Friederike

Pflugers Archiv : European journal of physiology

2021 Volume 473, Issue 12, Page(s) 1859–1884

Abstract: Mutations of TRPV3 lead to severe dermal hyperkeratosis in Olmsted syndrome, but whether the mutants are trafficked to the cell membrane or not is controversial. Even less is known about TRPV3 function in intestinal epithelia, although research on ... ...

Abstract	Mutations of TRPV3 lead to severe dermal hyperkeratosis in Olmsted syndrome, but whether the mutants are trafficked to the cell membrane or not is controversial. Even less is known about TRPV3 function in intestinal epithelia, although research on ruminants and pigs suggests an involvement in the uptake of NH
MeSH term(s)	Ammonia/metabolism ; Animals ; Biological Transport/physiology ; Calcium Signaling/physiology ; Cell Line ; Cell Membrane/metabolism ; Cell Membrane Permeability/physiology ; Epithelium/metabolism ; HEK293 Cells ; Humans ; Hydrogen-Ion Concentration ; Keratinocytes/metabolism ; Mutation/physiology ; Oocytes/metabolism ; Patch-Clamp Techniques/methods ; TRPV Cation Channels/metabolism ; Xenopus laevis/metabolism
Chemical Substances	TRPV Cation Channels ; TRPV3 protein, human ; Ammonia (7664-41-7)
Language	English
Publishing date	2021-10-19
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	6380-0
ISSN	1432-2013 ; 0031-6768
ISSN (online)	1432-2013
ISSN	0031-6768
DOI	10.1007/s00424-021-02616-0
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Article ; Online: The TRPV3 channel of the bovine rumen: localization and functional characterization of a protein relevant for ruminal ammonia transport.

Liebe, Franziska / Liebe, Hendrik / Kaessmeyer, Sabine / Sponder, Gerhard / Stumpff, Friederike

Pflugers Archiv : European journal of physiology

2020 Volume 472, Issue 6, Page(s) 693–710

Abstract: Large quantities of ammonia ( ... ...

Abstract	Large quantities of ammonia (NH
MeSH term(s)	Ammonia/metabolism ; Animals ; Biological Transport/physiology ; Cations/metabolism ; Cattle ; Cell Line ; Epithelium/metabolism ; HEK293 Cells ; Humans ; Hydrogen-Ion Concentration ; Permeability ; Rumen/metabolism ; Sodium/metabolism ; TRPV Cation Channels/metabolism ; Xenopus laevis/metabolism
Chemical Substances	Cations ; TRPV Cation Channels ; Ammonia (7664-41-7) ; Sodium (9NEZ333N27)
Language	English
Publishing date	2020-05-26
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	6380-0
ISSN	1432-2013 ; 0031-6768
ISSN (online)	1432-2013
ISSN	0031-6768
DOI	10.1007/s00424-020-02393-2
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Article ; Online: The Combined Influence of Magnesium and Insulin on Central Metabolic Functions and Expression of Genes Involved in Magnesium Homeostasis of Cultured Bovine Adipocytes.

Becker, Sandra K / Sponder, Gerhard / Sandhu, Mansur A / Trappe, Susanne / Kolisek, Martin / Aschenbach, Jörg R

International journal of molecular sciences

2021 Volume 22, Issue 11

Abstract: At the onset of lactation, dairy cows suffer from insulin resistance, insulin deficiency or both, similar to human diabetes, resulting in lipolysis, ketosis and fatty liver. This work explored the combined effects of different levels of magnesium (0.1, 0. ...

Abstract	At the onset of lactation, dairy cows suffer from insulin resistance, insulin deficiency or both, similar to human diabetes, resulting in lipolysis, ketosis and fatty liver. This work explored the combined effects of different levels of magnesium (0.1, 0.3, 1 and 3 mM) and insulin (25, 250 and 25,000 pM) on metabolic pathways and the expression of magnesium-responsive genes in a bovine adipocyte model. Magnesium starvation (0.1 mM) and low insulin (25 pM) independently decreased or tended to decrease the accumulation of non-polar lipids and uptake of the glucose analog 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-6-deoxyglucose (6-NBDG). Activity of glycerol 3-phosphate dehydrogenase (GPDH) was highest at 25 pM insulin and 3 mM magnesium. Expression of
MeSH term(s)	Adipocytes/drug effects ; Adipocytes/metabolism ; Animals ; Cattle ; Cells, Cultured ; Energy Metabolism/drug effects ; Gene Expression Regulation/drug effects ; Glucose/metabolism ; Homeostasis ; Insulin/metabolism ; Insulin/pharmacology ; Lipid Metabolism/drug effects ; Magnesium/metabolism ; Magnesium/pharmacology ; NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases/metabolism
Chemical Substances	Insulin ; NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases (EC 1.1.1.-) ; Magnesium (I38ZP9992A) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2021-05-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22115897
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Article: Expression of glucose and magnesium transport-associated genes in whole blood RNA of lactating ewes supplemented with magnesium

Ahmed, Mona H / Sponder, Gerhard / Wilkens, Mirja R / Schuberth, Hans-Joachim / Ganter, Martin / Breves, Gerhard / Aschenbach, Jörg R

Livestock science. 2021 Aug., v. 250

2021

Abstract: Lactation is characterized by an increase in energy demand and glucose consumption, which may also be linked to the concurrent compromise in immune function during early lactation. Magnesium (Mg) could be involved in this link based on its role for more ... ...

Abstract	Lactation is characterized by an increase in energy demand and glucose consumption, which may also be linked to the concurrent compromise in immune function during early lactation. Magnesium (Mg) could be involved in this link based on its role for more than 600 metabolic reactions associated with energy metabolism, in addition to its essential role in glucose metabolism. Therefore, the current study aimed to investigate the influence of Mg supplementation on the expression of glucose and Mg transport-associated genes in blood cells (predominantly leukocytes) of lactating ewes and to identify possible correlations between the expression of these genes and leukocyte subpopulations. Pregnant ewes were divided into a control group (Control, n = 8) and a Mg group (Mg, n = 8), the latter supplemented with magnesium oxide at a daily Mg intake of approximately 0.30% and 0.38% of dry matter during the last four weeks of gestation and the first four weeks after lambing, respectively. At the end of the supplementation period, blood samples were collected in PAXgene tubes in order to determine the mRNA expression of glucose transporters (GLUT1, GLUT3 and GLUT4) and genes linked to Mg transport (SLC41A1, CNNM2, TRPM6, TRPM7 and MagT1) in blood cells. Mg supplementation tended to increase the expression of GLUT1 in blood cells (P = 0.087). Expression values of GLUT3, SLC41A1, CNNM2, TRPM6, TRPM7, and MagT1 were not significantly changed (P > 0.1). Interestingly, GLUT4 was not detected in either group. Counts of circulating neutrophils, lymphocytes and monocytes showed no significant differences between the two groups. Furthermore, no correlations were observed between the expression of the investigated genes and specific leukocyte subpopulation percentages/frequenciies. In conclusion, the expression of a variety of glucose and Mg transport-associated genes did not respond to dietary Mg supplementation. Nonetheless, a tendency towards higher expression of GLUT1 in the Mg group might support an involvement of Mg in the regulation of glucose metabolism of ovine blood cells.
Keywords	early lactation ; energy ; energy metabolism ; gene expression ; glucose ; immune response ; magnesium ; magnesium oxide ; monocytes ; neutrophils ; pregnancy
Language	English
Dates of publication	2021-08
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2226176-X
ISSN	1878-0490 ; 1871-1413
ISSN (online)	1878-0490
ISSN	1871-1413
DOI	10.1016/j.livsci.2021.104583
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Article: Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine.

Trachsel, Dagmar S / Stage, Hannah J / Rausch, Sebastian / Trappe, Susanne / Söllig, Katharina / Sponder, Gerhard / Merle, Roswitha / Aschenbach, Jörg R / Gehlen, Heidrun

Animals : an open access journal from MDPI

2022 Volume 12, Issue 16

Abstract: Physiological particularities of the equine heart justify the development of an in vitro model suitable for investigations of the species-specific equine cardiac electrophysiology. Adipose tissue-derived stromal/stem cells (ASCs) could be a promising ... ...

Abstract	Physiological particularities of the equine heart justify the development of an in vitro model suitable for investigations of the species-specific equine cardiac electrophysiology. Adipose tissue-derived stromal/stem cells (ASCs) could be a promising starting point from which to develop such a cardiomyocyte (CM)-like cell model. Therefore, we compared abdominal, retrobulbar, and subcutaneous adipose tissue as sources for the isolation of ASCs applying two isolation methods: the collagenase digestion and direct explant culture. Abdominal adipose tissue was most suitable for the isolation of ASCs and both isolation methods resulted in comparable yields of CD45-/CD34-negative cells expressing the mesenchymal stem cell markers CD29, CD44, and CD90, as well as pluripotency markers, as determined by flow cytometry and real-time quantitative PCR. However, exposure of equine ASCs to 5-azacytidine (5-AZA), reportedly inducing CM differentiation from rats, rabbits, and human ASCs, was not successful in our study. More precisely, neither the early differentiation markers
Language	English
Publishing date	2022-08-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani12162049
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Article: Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine

Trachsel, Dagmar S. / Stage, Hannah J. / Rausch, Sebastian / Trappe, Susanne / Söllig, Katharina / Sponder, Gerhard / Merle, Roswitha / Aschenbach, Jörg R. / Gehlen, Heidrun

Animals. 2022 Aug. 11, v. 12, no. 16

2022

Abstract	Physiological particularities of the equine heart justify the development of an in vitro model suitable for investigations of the species-specific equine cardiac electrophysiology. Adipose tissue-derived stromal/stem cells (ASCs) could be a promising starting point from which to develop such a cardiomyocyte (CM)-like cell model. Therefore, we compared abdominal, retrobulbar, and subcutaneous adipose tissue as sources for the isolation of ASCs applying two isolation methods: the collagenase digestion and direct explant culture. Abdominal adipose tissue was most suitable for the isolation of ASCs and both isolation methods resulted in comparable yields of CD45-/CD34-negative cells expressing the mesenchymal stem cell markers CD29, CD44, and CD90, as well as pluripotency markers, as determined by flow cytometry and real-time quantitative PCR. However, exposure of equine ASCs to 5-azacytidine (5-AZA), reportedly inducing CM differentiation from rats, rabbits, and human ASCs, was not successful in our study. More precisely, neither the early differentiation markers GATA4 and NKX2-5, nor the late CM differentiation markers TNNI3, MYH6, and MYH7 were upregulated in equine ASCs exposed to 10 µM 5-AZA for 48 h. Hence, further work focusing on the optimal conditions for CM differentiation of equine stem cells derived from adipose tissue, as well as possibly from other origins, are needed.
Keywords	GATA transcription factors ; adipose tissue ; cardiomyocytes ; collagenase ; digestion ; electrophysiology ; flow cytometry ; horses ; humans ; mesenchymal stromal cells ; models ; quantitative polymerase chain reaction
Language	English
Dates of publication	2022-0811
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani12162049
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Article: Erratum to "The GadX regulon affects virulence gene expression and adhesion of porcine enteropathogenic

Braun, Hannah-Sophie / Sponder, Gerhard / Aschenbach, Jörg R / Kerner, Katharina / Bauerfeind, Rolf / Deiner, Carolin

Veterinary and animal science

2020 Volume 9, Page(s) 100093

Abstract: This corrects the article DOI: 10.1016/j.vas.2017.04.001.]. ...

Abstract	[This corrects the article DOI: 10.1016/j.vas.2017.04.001.].
Keywords	covid19
Language	English
Publishing date	2020-01-10
Publishing country	United States
Document type	Published Erratum
ISSN	2451-943X
ISSN	2451-943X
DOI	10.1016/j.vas.2020.100093
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Article: Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources

Rashid, Usman / Yousaf, Arfan / Yaqoob, Muhammad / Saba, Evelyn / Moaeen-ud-Din, Muhammad / Waseem, Shahid / Becker, Sandra K. / Sponder, Gerhard / Aschenbach, Jörg R. / Sandhu, Mansur Abdullah

BMC veterinary research. 2021 Dec., v. 17, no. 1

2021

Abstract: BACKGROUND: Mesenchymal stem cells (MSCs) are undifferentiated cells that can give rise to a mesoderm lineage. Adipose-derived MSCs are an easy and accessible source for MSCs isolation, although each source of MSC has its own advantages and disadvantages. ...

Abstract	BACKGROUND: Mesenchymal stem cells (MSCs) are undifferentiated cells that can give rise to a mesoderm lineage. Adipose-derived MSCs are an easy and accessible source for MSCs isolation, although each source of MSC has its own advantages and disadvantages. Our study identifies a promising source for the isolation and differentiation of canines MSCs. For this purpose, adipose tissue from inguinal subcutaneous (SC), perirenal (PR), omental (OM), and infrapatellar fat pad (IPFP) was isolated and processed for MSCs isolation. In the third passage, MSCs proliferation/metabolism, surface markers expression, in vitro differentiation potential and quantitative reverse transcription PCR (CD73, CD90, CD105, PPARγ, FabP4, FAS, SP7, Osteopontin, and Osteocalcin) were evaluated. RESULTS: Our results showed that MSCs derived from IPFP have a higher proliferation rate, while OM-derived MSCs have higher cell metabolism. In addition, MSCs from all adipose tissue sources showed positive expression of CD73 (NT5E), CD90 (THY1), CD105 (ENDOGLIN), and very low expression of CD45. The isolated canine MSCs were successfully differentiated into adipogenic and osteogenic lineages. The oil-red-O quantification and adipogenic gene expression (FAS, FabP4, and PPARγ) were higher in OM-derived cells, followed by IPFP-MSCs. Similarly, in osteogenic differentiation, alkaline phosphatase activity and osteogenic gene (SP7 and Osteocalcin) expression were higher in OM-derived MSCs, while osteopontin expression was higher in PR-derived MSCs. CONCLUSION: In summary, among all four adipose tissue sources, OM-derived MSCs have better differentiation potential toward adipo- and osteogenic lineages, followed by IPFP-MSCs. Interestingly, among all adipose tissue sources, MSCs derived from IPFP have the maximum proliferation potential. The characterization and differentiation potential of canine MSCs isolated from four different adipose tissue sources are useful to assess their potential for application in regenerative medicine.
Keywords	adipose tissue ; alkaline phosphatase ; bone formation ; dogs ; gene expression ; genes ; medicine ; mesoderm ; metabolism ; osteocalcin ; osteopontin ; reverse transcriptase polymerase chain reaction ; veterinary medicine
Language	English
Dates of publication	2021-12
Size	p. 388.
Publishing place	BioMed Central
Document type	Article
ZDB-ID	2191675-5
ISSN	1746-6148
ISSN	1746-6148
DOI	10.1186/s12917-021-03100-8
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