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  1. Article: Ex-situ biological CO2 methanation using trickle bed reactor: review and recent advances

    Sposob, Michal / Wahid, Radziah / Fischer, Keno

    Re/views in environmental science and bio/technology. 2021 Dec., v. 20, no. 4

    2021  

    Abstract: Power-to-methane technology is a promising solution to facilitate the use of excess variable renewable energy for biomethane production. In this approach, hydrogen produced via electrolysis is used to upgrade raw biogas, which can be subsequently used as ...

    Abstract Power-to-methane technology is a promising solution to facilitate the use of excess variable renewable energy for biomethane production. In this approach, hydrogen produced via electrolysis is used to upgrade raw biogas, which can be subsequently used as fuel or stored in the gas grid. Ex-situ biomethanation is an emerging technology that could potentially replace conventional energy-intensive biogas upgrading methods and allow CO₂ utilization for biomethane production. This work provides a comprehensive overview on the current status of ex-situ biomethanation with particular attention to trickle bed reactor. The review includes description of ex-situ biomethanation and summarizes previous works on this topic. The key elements related to operational conditions, efficiency, and microbiology of ex-situ biomethanation using trickle bed reactor are described here. Additionally, the review highlights the technical and economic issues that have to be addressed for future development and large-scale implementation of ex-situ biomethanation.
    Keywords biogas ; carbon dioxide ; electrolysis ; environmental science ; hydrogen ; methane production ; microbiology ; technology
    Language English
    Dates of publication 2021-12
    Size p. 1087-1102.
    Publishing place Springer Netherlands
    Document type Article
    Note Review
    ZDB-ID 2076953-2
    ISSN 1572-9826 ; 1569-1705
    ISSN (online) 1572-9826
    ISSN 1569-1705
    DOI 10.1007/s11157-021-09589-7
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Bicarbonate for microalgae cultivation: a case study in a chlorophyte, Tetradesmus wisconsinensis isolated from a Norwegian lake

    Umetani, Ikumi / Janka, Eshetu / Sposób, Michal / Hulatt, Chris J / Kleiven, Synne / Bakke, Rune

    Journal of applied phycology. 2021 June, v. 33, no. 3

    2021  

    Abstract: Bicarbonate was evaluated as an alternative carbon source for a green microalga, Tetradesmus wisconsinensis, isolated from Lake Norsjø in Norway. Photosynthesis, growth, and lipid production were studied using four inorganic carbon regimes: (1) aeration ... ...

    Abstract Bicarbonate was evaluated as an alternative carbon source for a green microalga, Tetradesmus wisconsinensis, isolated from Lake Norsjø in Norway. Photosynthesis, growth, and lipid production were studied using four inorganic carbon regimes: (1) aeration only, (2) 20 mM NaHCO₃, (3) 5% (v/v) CO₂ gas, and (4) combination of 20 mM NaHCO₃ and 5% CO₂. Variable chlorophyll a fluorescence analysis revealed that the bicarbonate treatment supported effective photosynthesis, while the CO₂ treatment led to inefficient photosynthetic activity with a PSII maximum quantum yield as low as 0.31. Conversely, bicarbonate and CO₂ treatments gave similar biomass and fatty acid production. The maximum growth rate, the final cell dry weight, and total fatty acids under the bicarbonate-only treatment were 0.33 (± 0.06) day⁻¹, 673 (± 124) mg L⁻¹ and 75 (± 5) mg g⁻¹ dry biomass, respectively. The most abundant fatty acid components were α-linolenic acid and polyunsaturated fatty acids constituting 69% of the total fatty acids. The fatty acid profile eventuated in unsuitable biodiesel fuel properties such as high degree of unsaturation and low cetane number; however, it would be relevant for food and feed applications. We concluded that bicarbonate could give healthy growth and comparative product yields as CO₂.
    Keywords Tetradesmus ; aeration ; algology ; biodiesel ; biomass ; carbon dioxide ; case studies ; chlorophyll ; fatty acid composition ; fluorescence ; inorganic carbon ; lakes ; microalgae ; photosystem II ; Norway
    Language English
    Dates of publication 2021-06
    Size p. 1341-1352.
    Publishing place Springer Netherlands
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1002324-0
    ISSN 1573-5176 ; 0921-8971
    ISSN (online) 1573-5176
    ISSN 0921-8971
    DOI 10.1007/s10811-021-02420-4
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  3. Article: Comprehensive analysis of the microbial communities and operational parameters of two full-scale anaerobic digestion plants treating food waste in South Korea: Seasonal variation and effect of ammonia

    Sposob, Michal / Moon, Hee-Sung / Lee, Dongjin / Kim, Tae-Hoon / Yun, Yeo-Myeong

    Journal of hazardous materials. 2020 Nov. 05, v. 398

    2020  

    Abstract: There are about ninety full-scale anaerobic digestion (AD) plants in South Korea that treat food waste (FW); however, the key diff ;erences in the microbial communities in different seasons and the effects of ammonia in AD remain poorly understood. In ... ...

    Abstract There are about ninety full-scale anaerobic digestion (AD) plants in South Korea that treat food waste (FW); however, the key diff ;erences in the microbial communities in different seasons and the effects of ammonia in AD remain poorly understood. In this study, the seasonal changes in microbial communities associated with operational parameters of two full-scale ADs (C and W plants) treating FW were analyzed. The organic loading rate (OLR) variability had an influence on the seasonal CH₄ yield; the W plant had a lower CH₄ yield with an unstable AD performance while the C plant had a higher CH₄ yield with a stable AD performance. It was mainly due to the substantially different NH₄⁺ concentration; the W plant had a NH₄⁺ concentration nearly 1.6 times higher compared to the C plant. The high NH₄⁺ presence in the W plant led to the dominance of class Clostridia, and methanogenesis was mostly done by hydrogenotrophs (Methanomassiliicoccus luminyensis). Additionally, the members belonging to Clostridia and Bacteroidia were found at both plants in each season (share ≥0.5%) implying their indispensable role during the anaerobic digestion of FW.
    Keywords Bacteroidia ; Clostridia ; ammonia ; anaerobic digestion ; food waste ; methane production ; seasonal variation ; South Korea
    Language English
    Dates of publication 2020-1105
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1491302-1
    ISSN 1873-3336 ; 0304-3894
    ISSN (online) 1873-3336
    ISSN 0304-3894
    DOI 10.1016/j.jhazmat.2020.122975
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  4. Article: Effect of a side-stream voltage supplied by sludge recirculation to an anaerobic digestion reactor

    Park, Jun-Gyu / Kwon, Hye-Jeong / Sposob, Michal / Jun, Hang-Bae

    Bioresource technology. 2020 Mar., v. 300

    2020  

    Abstract: This study showed that side stream voltage supplied by sludge recirculation from an auxiliary bio-electrochemical anaerobic digestion (ABEAD) reactor appears to have a similar effect as main stream voltage supply to an anaerobic digestion (AD) reactor. ... ...

    Abstract This study showed that side stream voltage supplied by sludge recirculation from an auxiliary bio-electrochemical anaerobic digestion (ABEAD) reactor appears to have a similar effect as main stream voltage supply to an anaerobic digestion (AD) reactor. The increased sludge recirculation rate enhanced the operation stability at a high OLR. H2-producing bacterial community was improved in bio-electrochemical anaerobic digestion (BEAD) and ABEAD reactors and was increased with increase in sludge recirculation rate. Despite the dominance of hydrogenotrophic methanogens in all reactors, high operational performances of BEAD and ABEAD reactors supports the results of H2-producing bacteria increase in those reactors. The ABEAD reactors having 1/7 of the capacity of the main AD reactor showed possibility of integration of BEAD technology into new and existing facilities economically. The findings of this study would provide useful information for approaching the commercialization of BEAD and suggest direction of further research for practical applications.
    Keywords anaerobic digestion ; bacteria ; bacterial communities ; commercialization ; methanogens ; sludge
    Language English
    Dates of publication 2020-03
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2019.122643
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  5. Article: Assessment of the relationship between solubilization and biogas production on anaerobic digestion of pretreated lipid-extracted microalgae waste

    Sposob, Michal / Kim, Dong-Hoon / Yun, Gwang-Sue / Yun, Yeo-Myeong

    Biomass and bioenergy. 2020 Oct., v. 141

    2020  

    Abstract: It is commonly known that the pretreatment of complex substrates yields higher biogas production in anaerobic digestion (AD) by improving hydrolysis. However, it is still questioned whether all solubilized fractions after pretreatment can be used for CH₄ ...

    Abstract It is commonly known that the pretreatment of complex substrates yields higher biogas production in anaerobic digestion (AD) by improving hydrolysis. However, it is still questioned whether all solubilized fractions after pretreatment can be used for CH₄ production during AD. In this study, the relationship between increased solubilization and AD efficiency in response to different pretreatment conditions of lipid-extracted microalgae waste (LEMW) was investigated. The individual pretreatment (acid and ultrasonic) and combined pretreatment were applied to assess the solubilization of LEMW. A biochemical methane potential (BMP) test was subsequently performed to determine the AD efficiency. Combined pretreatment of LEMW (60 min of irradiation + pH 1) showed the highest performance, achieving CH₄ production of 1245 ± 28 mL CH₄/L with increased solubilization of 50.4%. However, it was found that increased solubilization did not proportionally increase CH₄ productivity. The assessment of the origin of produced CH₄ through biomass fractionation supports this finding in that the soluble fraction that does not contribute to CH₄ production increased at more severe pretreatment conditions.
    Keywords anaerobic digestion ; bioenergy ; biomass ; fractionation ; gas production (biological) ; hydrolysis ; irradiation ; methane ; microalgae ; pH ; solubilization ; ultrasonics ; wastes
    Language English
    Dates of publication 2020-10
    Publishing place Elsevier Ltd
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1090121-8
    ISSN 0961-9534
    ISSN 0961-9534
    DOI 10.1016/j.biombioe.2020.105702
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Effect of a side-stream voltage supplied by sludge recirculation to an anaerobic digestion reactor.

    Park, Jun-Gyu / Kwon, Hye-Jeong / Sposob, Michal / Jun, Hang-Bae

    Bioresource technology

    2019  Volume 300, Page(s) 122643

    Abstract: This study showed that side stream voltage supplied by sludge recirculation from an auxiliary bio-electrochemical anaerobic digestion (ABEAD) reactor appears to have a similar effect as main stream voltage supply to an anaerobic digestion (AD) reactor. ... ...

    Abstract This study showed that side stream voltage supplied by sludge recirculation from an auxiliary bio-electrochemical anaerobic digestion (ABEAD) reactor appears to have a similar effect as main stream voltage supply to an anaerobic digestion (AD) reactor. The increased sludge recirculation rate enhanced the operation stability at a high OLR. H
    MeSH term(s) Anaerobiosis ; Bioreactors ; Euryarchaeota ; Methane ; Sewage
    Chemical Substances Sewage ; Methane (OP0UW79H66)
    Language English
    Publishing date 2019-12-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2019.122643
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  7. Article: Effects of N/S Molar Ratio on Product Formation in Psychrophilic Autotrophic Biological Removal of Sulfide

    Sposob, Michal / Bakke, Rune / Dinamarca, Carlos

    Water. 2017 June 29, v. 9, no. 7

    2017  

    Abstract: The excessive H ... 2 ... S presence in water and wastewater can lead to corrosion, toxicity, and biological processes inhibition—i.e., anaerobic digestion. Production of H ... 2 ... S can occur in psychrophilic conditions. Biological removal of HS− by ...

    Abstract The excessive H<inf>2</inf>S presence in water and wastewater can lead to corrosion, toxicity, and biological processes inhibition—i.e., anaerobic digestion. Production of H<inf>2</inf>S can occur in psychrophilic conditions. Biological removal of HS− by addition of NO<inf>3</inf>− as an electron acceptor under psychrophilic (10 °C) conditions in a continuous flow experiment is evaluated here. Four different N/S molar ratios—0.35, 0.40, 0.60, and 1.30—were tested in an expanded granular sludge bed (EGSB) reactor. Samples were analyzed daily by ion chromatography. Efficient psychrophilic HS− removal with sulfur products oxidation control by NO<inf>3</inf>− supply is documented. The highest HS− removal was obtained at N/S = 0.35 and 1.30 (89.1 ± 2.2 and 89.6 ± 2.9%). Removal of HS− was less at mid-N/S with the lowest value (76.9 ± 2.6%) at N/S = 0.60. NO<inf>3</inf>− removal remained high for all N/S ratios. N/S molar ratio influenced the sulfur products distribution with less S0 and increase in SO<inf>4</inf>2− effluent concentration with increasing N/S ratio. Oxidation of HS− and accumulated S0 occurred simultaneously at N/S ratios >0.35. The observations are explained by culture flexibility in utilizing available resources for energy gain.
    Keywords anaerobic digestion ; corrosion ; energy ; hydrogen sulfide ; ion exchange chromatography ; nitrates ; oxidation ; sludge ; sulfates ; sulfur ; toxicity ; wastewater
    Language English
    Dates of publication 2017-0629
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2521238-2
    ISSN 2073-4441
    ISSN 2073-4441
    DOI 10.3390/w9070476
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Metabolic divergence in simultaneous biological removal of nitrate and sulfide for elemental sulfur production under temperature stress

    Sposob, Michal / Carlos Dinamarca / Rune Bakke

    Bioresource technology. 2017 June, v. 233

    2017  

    Abstract: The simultaneous removal of NO3− and HS− at temperature stress (25–10°C) is evaluated here. An expanded granular sludge bed (EGSB) reactor was run over 120days at N/S molar ratio of 0.35 (for S0 production) under constant sulfur loading rate of 0.4kgS/ ... ...

    Abstract The simultaneous removal of NO3− and HS− at temperature stress (25–10°C) is evaluated here. An expanded granular sludge bed (EGSB) reactor was run over 120days at N/S molar ratio of 0.35 (for S0 production) under constant sulfur loading rate of 0.4kgS/m3d. The simultaneous removal of NO3− and HS−, was achieved at applied conditions. Average HS−-S removal varied from 98 (25°C) to 89.2% at 10°C, with almost complete NO3− removal. Average S0 yield ranged from 83.7 at 25°C to 67% at 10°C. The temperature drop caused a decrease in granular sludge accumulated S0 fraction by nearly 2.5 times. Decreased temperature caused metabolic pathway change observed as higher SO42− production, apparently allowing the biomass to obtain more energy per HS− consumed. It is hypothesized that the metabolic shift is a natural response to compensate for temperature-induced changes in energy requirements.
    Keywords biochemical pathways ; biomass ; energy ; energy requirements ; nitrates ; sludge ; sulfates ; sulfides ; sulfur ; temperature
    Language English
    Dates of publication 2017-06
    Size p. 209-215.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2017.02.122
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  9. Article ; Online: Comprehensive analysis of the microbial communities and operational parameters of two full-scale anaerobic digestion plants treating food waste in South Korea: Seasonal variation and effect of ammonia.

    Sposob, Michal / Moon, Hee-Sung / Lee, Dongjin / Kim, Tae-Hoon / Yun, Yeo-Myeong

    Journal of hazardous materials

    2020  Volume 398, Page(s) 122975

    Abstract: There are about ninety full-scale anaerobic digestion (AD) plants in South Korea that treat food waste (FW); however, the key diff ;erences in the microbial communities in different seasons and the effects of ammonia in AD remain poorly understood. In ... ...

    Abstract There are about ninety full-scale anaerobic digestion (AD) plants in South Korea that treat food waste (FW); however, the key diff ;erences in the microbial communities in different seasons and the effects of ammonia in AD remain poorly understood. In this study, the seasonal changes in microbial communities associated with operational parameters of two full-scale ADs (C and W plants) treating FW were analyzed. The organic loading rate (OLR) variability had an influence on the seasonal CH
    MeSH term(s) Ammonia ; Anaerobiosis ; Bioreactors ; Food ; Methane ; Microbiota ; Refuse Disposal ; Republic of Korea ; Seasons
    Chemical Substances Ammonia (7664-41-7) ; Methane (OP0UW79H66)
    Language English
    Publishing date 2020-05-20
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1491302-1
    ISSN 1873-3336 ; 0304-3894
    ISSN (online) 1873-3336
    ISSN 0304-3894
    DOI 10.1016/j.jhazmat.2020.122975
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  10. Article ; Online: Metabolic divergence in simultaneous biological removal of nitrate and sulfide for elemental sulfur production under temperature stress.

    Sposob, Michal / Bakke, Rune / Dinamarca, Carlos

    Bioresource technology

    2017  Volume 233, Page(s) 209–215

    Abstract: The simultaneous removal of ... ...

    Abstract The simultaneous removal of NO
    MeSH term(s) Bioreactors ; Nitrates/metabolism ; Sulfides/metabolism ; Sulfur/metabolism ; Temperature
    Chemical Substances Nitrates ; Sulfides ; Sulfur (70FD1KFU70)
    Language English
    Publishing date 2017-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2017.02.122
    Database MEDical Literature Analysis and Retrieval System OnLINE

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