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  1. AU="Sprent, Noah"
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  1. Artikel ; Online: Functional Synthetic Biology.

    Aldulijan, Ibrahim / Beal, Jacob / Billerbeck, Sonja / Bouffard, Jeff / Chambonnier, Gaël / Ntelkis, Nikolaos / Guerreiro, Isaac / Holub, Martin / Ross, Paul / Selvarajah, Vinoo / Sprent, Noah / Vidal, Gonzalo / Vignoni, Alejandro

    Synthetic biology (Oxford, England)

    2023  Band 8, Heft 1, Seite(n) ysad006

    Abstract: Synthetic biologists have made great progress over the past decade in developing methods for modular assembly of genetic sequences and in engineering biological systems with a wide variety of functions in various contexts and organisms. However, current ... ...

    Abstract Synthetic biologists have made great progress over the past decade in developing methods for modular assembly of genetic sequences and in engineering biological systems with a wide variety of functions in various contexts and organisms. However, current paradigms in the field entangle sequence and functionality in a manner that makes abstraction difficult, reduces engineering flexibility and impairs predictability and design reuse. Functional Synthetic Biology aims to overcome these impediments by focusing the design of biological systems on function, rather than on sequence. This reorientation will decouple the engineering of biological devices from the specifics of how those devices are put to use, requiring both conceptual and organizational change, as well as supporting software tooling. Realizing this vision of Functional Synthetic Biology will allow more flexibility in how devices are used, more opportunity for reuse of devices and data, improvements in predictability and reductions in technical risk and cost.
    Sprache Englisch
    Erscheinungsdatum 2023-04-08
    Erscheinungsland England
    Dokumenttyp Journal Article ; Review
    ISSN 2397-7000
    ISSN (online) 2397-7000
    DOI 10.1093/synbio/ysad006
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Buch ; Online: Functional Synthetic Biology

    Aldulijan, Ibrahim / Beal, Jacob / Billerbeck, Sonja / Bouffard, Jeff / Chambonnier, Gael / Delkis, Nikolaos / Guerreiro, Isaac / Holub, Martin / Ross, Paul / Selvarajah, Vinoo / Sprent, Noah / Vidal, Gonzalo / Vignoni, Alejandro

    2022  

    Abstract: Synthetic biologists have made great progress over the past decade in developing methods for modular assembly of genetic sequences and in engineering biological systems with a wide variety of functions in various contexts and organisms. However, current ... ...

    Abstract Synthetic biologists have made great progress over the past decade in developing methods for modular assembly of genetic sequences and in engineering biological systems with a wide variety of functions in various contexts and organisms. However, current paradigms in the field entangle sequence and functionality in a manner that makes abstraction difficult, reduces engineering flexibility, and impairs predictability and design reuse. Functional Synthetic Biology aims to overcome these impediments by focusing the design of biological systems on function, rather than on sequence. This reorientation will decouple the engineering of biological devices from the specifics of how those devices are put to use, requiring both conceptual and organizational change, as well as supporting software tooling. Realizing this vision of Functional Synthetic Biology will allow more flexibility in how devices are used, more opportunity for reuse of devices and data, improvements in predictability, and reductions in technical risk and cost.

    Comment: 8 pages, 4 figures; submitted to OUP Synthetic Biology
    Schlagwörter Quantitative Biology - Quantitative Methods
    Thema/Rubrik (Code) 501
    Erscheinungsdatum 2022-07-01
    Erscheinungsland us
    Dokumenttyp Buch ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

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  3. Artikel ; Online: Multicolor plate reader fluorescence calibration.

    Beal, Jacob / Telmer, Cheryl A / Vignoni, Alejandro / Boada, Yadira / Baldwin, Geoff S / Hallett, Liam / Lee, Taeyang / Selvarajah, Vinoo / Billerbeck, Sonja / Brown, Bradley / Cai, Guo-Nan / Cai, Liang / Eisenstein, Edward / Kiga, Daisuke / Ross, David / Alperovich, Nina / Sprent, Noah / Thompson, Jaclyn / Young, Eric M /
    Endy, Drew / Haddock-Angelli, Traci

    Synthetic biology (Oxford, England)

    2022  Band 7, Heft 1, Seite(n) ysac010

    Abstract: Plate readers are commonly used to measure cell growth and fluorescence, yet the utility and reproducibility of plate reader data is limited by the fact that it is typically reported in arbitrary or relative units. We have previously established a robust ...

    Abstract Plate readers are commonly used to measure cell growth and fluorescence, yet the utility and reproducibility of plate reader data is limited by the fact that it is typically reported in arbitrary or relative units. We have previously established a robust serial dilution protocol for calibration of plate reader measurements of absorbance to estimated bacterial cell count and for green fluorescence from proteins expressed in bacterial cells to molecules of equivalent fluorescein. We now extend these protocols to calibration of red fluorescence to the sulforhodamine-101 fluorescent dye and blue fluorescence to Cascade Blue. Evaluating calibration efficacy via an interlaboratory study, we find that these calibrants do indeed provide comparable precision to the prior calibrants and that they enable effective cross-laboratory comparison of measurements of red and blue fluorescence from proteins expressed in bacterial cells.
    Sprache Englisch
    Erscheinungsdatum 2022-08-06
    Erscheinungsland England
    Dokumenttyp Journal Article
    ISSN 2397-7000
    ISSN (online) 2397-7000
    DOI 10.1093/synbio/ysac010
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

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