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Article ; Online: 1

Dwivedi, Navneet / Siddiqui, Mohd Adnan / Srivastava, Seema / Sinha, Neeraj

2023 Volume 61, Issue 7, Page(s) 397–406

Abstract: Bone is a living tissue made up of organic proteins, inorganic minerals, and water. The organic component of bone (mainly made up of Type-I collagen) provides flexibility and tensile strength. Solid-state nuclear magnetic resonance (ssNMR) is one of the ... ...

Abstract	Bone is a living tissue made up of organic proteins, inorganic minerals, and water. The organic component of bone (mainly made up of Type-I collagen) provides flexibility and tensile strength. Solid-state nuclear magnetic resonance (ssNMR) is one of the few techniques that can provide atomic-level structural insights of such biomaterials in their native state. In the present article, we employed the variable contact time cross-polarization (
MeSH term(s)	Kinetics ; Bone and Bones ; Extracellular Matrix/metabolism ; Collagen/chemistry ; Water/chemistry
Chemical Substances	Collagen (9007-34-5) ; Water (059QF0KO0R)
Language	English
Publishing date	2023-03-29
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1475029-6
ISSN	1097-458X ; 0749-1581
ISSN (online)	1097-458X
ISSN	0749-1581
DOI	10.1002/mrc.5347
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Article ; Online: Unraveling Water-Mediated

Dwivedi, Navneet / Dubey, Richa / Srivastava, Seema / Sinha, Neeraj

ACS omega

2022 Volume 7, Issue 19, Page(s) 16678–16688

Abstract: Bone is a dynamic tissue composed of organic proteins (mainly type I collagen), inorganic components (hydroxyapatite), lipids, and water that undergoes a continuous rebuilding process over the lifespan of human beings. Bone mineral is mainly composed of ... ...

Abstract	Bone is a dynamic tissue composed of organic proteins (mainly type I collagen), inorganic components (hydroxyapatite), lipids, and water that undergoes a continuous rebuilding process over the lifespan of human beings. Bone mineral is mainly composed of a crystalline apatitic core surrounded by an amorphous surface layer. The supramolecular arrangement of different constituents gives rise to its unique mechanical properties, which become altered in various bone-related disease conditions. Many of the interactions among the different components are poorly understood. Recently, solid-state nuclear magnetic resonance (ssNMR) has become a popular spectroscopic tool for studying bone. In this article, we present a study probing the interaction of water molecules with amorphous and crystalline parts of the bone mineral through
Language	English
Publishing date	2022-05-02
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.2c01133
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Article ; Online: Dose exposure of Bisphenol- A on female Wistar rats fertility.

Srivastava, Seema / Dhagga, Nupoor

Hormone molecular biology and clinical investigation

2019 Volume 38, Issue 2

Abstract: Background 2, 2-Bis (4-hydroxyphenyl propane [bisphenol A (BPA)] is one of the major environmental pollutants and has the adverse effects on human health. BPA mimics the structure of estrogen and binds to estrogen receptors and alters the secretion of ... ...

Abstract	Background 2, 2-Bis (4-hydroxyphenyl propane [bisphenol A (BPA)] is one of the major environmental pollutants and has the adverse effects on human health. BPA mimics the structure of estrogen and binds to estrogen receptors and alters the secretion of the hormone. It is ingested in humans through the regular use of plastic containers, bottles and food cans. Materials and methods Female Wistar rats were exposed orally to 5, 50, 300, 600 and 800 mg BPA/kg body weight (bd. wt.)/week mixed in olive oil and administered every 168 h for 3 months continuing through the mating, gestation and lactation and its effects on fertility, reproductive organ weight and hormones [LH (luteinizing hormone), FSH (follicle stimulating hormone), estradiol (E2), progesterone (PROG) and PRL (prolactin)] were evaluated. Results The findings revealed that females exposed to BPA exhibited a decrease in female fertility rate and weight of reproductive organs (ovary and uterus) with significant decreased levels of LH, FSH, E2, PROG and PRL in the non-pregnancy phase whereas in cesarean and post-term females, no significance difference was found in fertility rate, reproductive organ weight and hormonal levels. Conclusions These data indicate an increased sensitivity to BPA needs careful evaluation of the current levels of exposure.
MeSH term(s)	Air Pollutants, Occupational/toxicity ; Animals ; Benzhydryl Compounds/toxicity ; Estradiol/blood ; Estrous Cycle/drug effects ; Female ; Follicle Stimulating Hormone/blood ; Infertility, Female/etiology ; Luteinizing Hormone/blood ; Ovary/drug effects ; Phenols/toxicity ; Progesterone/blood ; Rats ; Rats, Wistar ; Sexual Behavior, Animal/drug effects ; Uterus/drug effects
Chemical Substances	Air Pollutants, Occupational ; Benzhydryl Compounds ; Phenols ; Progesterone (4G7DS2Q64Y) ; Estradiol (4TI98Z838E) ; Luteinizing Hormone (9002-67-9) ; Follicle Stimulating Hormone (9002-68-0) ; bisphenol A (MLT3645I99)
Language	English
Publishing date	2019-05-07
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2536635-X
ISSN	1868-1891 ; 1868-1883
ISSN (online)	1868-1891
ISSN	1868-1883
DOI	10.1515/hmbci-2018-0061
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Article ; Online: Alteration in apoptotic rate of testicular cells and sperms following administration of Bisphenol A (BPA) in Wistar albino rats.

Srivastava, Seema / Gupta, Priya

Environmental science and pollution research international

2018 Volume 25, Issue 22, Page(s) 21635–21643

Abstract: The aim of the study was to evaluate the effect of Bisphenol A [BPA] widely used as a plasticizer in the formation of polycarbonate plastics and epoxy resins, exposure causing alteration in apoptosis rate, and protective effect of Vitamin E when ... ...

Abstract	The aim of the study was to evaluate the effect of Bisphenol A [BPA] widely used as a plasticizer in the formation of polycarbonate plastics and epoxy resins, exposure causing alteration in apoptosis rate, and protective effect of Vitamin E when supplemented with BPA orally. Adult male Wistar albino rats aged 3 months were randomly divided into seven groups: control (olive oil treated) BPA-treated (dose 5, 50,100 μg/100gmBW) and Vitamin E intervention group (dose 5, 50, 100 μg/100gmBW BPA+ Vitamin E dose 4 mg/100gmBW). Animals were sacrificed 3 months later, and blood and tissue samples were collected. Apoptotic changes were analyzed in epididymal spermatozoa and testis tissue by binding of annexin V apoptotic biomarker. A significant decline in the weight of testis, testosterone level, and sperm count was observed. Histopathological and apoptotic changes were observed in testis tissue. In epididymal sperms, the early apoptotic cells were observed by staining of annexin V-conjugated FITC and PI green fluorescence in spermatozoa head which indicated the damage of membrane and late apoptotic cells. These changes reduced significantly in Vitamin E-treated groups though were not found to be comparable to control animals. All these changes were attributed to disrupted spermatogenesis that would interfere with sperm formation. Thus, the study infers that BPA affects the apoptosis process in the testis and epididymal sperm that would interfere with its function and contribute to infertility, whereas Vitamin E-supplemented dose has a protective effect towards these changes, indicating its role in improving male fertility.
MeSH term(s)	Animals ; Apoptosis/drug effects ; Benzhydryl Compounds/toxicity ; Epididymis/drug effects ; Epididymis/pathology ; Male ; Organ Size/drug effects ; Phenols/toxicity ; Protective Agents/administration & dosage ; Protective Agents/pharmacology ; Rats, Wistar ; Sperm Count ; Spermatogenesis/drug effects ; Spermatozoa/drug effects ; Spermatozoa/pathology ; Testis/drug effects ; Testis/pathology ; Testosterone/metabolism ; Vitamin E/administration & dosage ; Vitamin E/pharmacology
Chemical Substances	Benzhydryl Compounds ; Phenols ; Protective Agents ; Vitamin E (1406-18-4) ; Testosterone (3XMK78S47O) ; bisphenol A (MLT3645I99)
Language	English
Publishing date	2018-05-21
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-018-2229-2
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Article: Alteration in apoptotic rate of testicular cells and sperms following administration of Bisphenol A (BPA) in Wistar albino rats

Srivastava, Seema / Priya Gupta

Environmental science and pollution research international. 2018 Aug., v. 25, no. 22

2018

Abstract	The aim of the study was to evaluate the effect of Bisphenol A [BPA] widely used as a plasticizer in the formation of polycarbonate plastics and epoxy resins, exposure causing alteration in apoptosis rate, and protective effect of Vitamin E when supplemented with BPA orally. Adult male Wistar albino rats aged 3 months were randomly divided into seven groups: control (olive oil treated) BPA-treated (dose 5, 50,100 μg/100gmBW) and Vitamin E intervention group (dose 5, 50, 100 μg/100gmBW BPA+ Vitamin E dose 4 mg/100gmBW). Animals were sacrificed 3 months later, and blood and tissue samples were collected. Apoptotic changes were analyzed in epididymal spermatozoa and testis tissue by binding of annexin V apoptotic biomarker. A significant decline in the weight of testis, testosterone level, and sperm count was observed. Histopathological and apoptotic changes were observed in testis tissue. In epididymal sperms, the early apoptotic cells were observed by staining of annexin V-conjugated FITC and PI green fluorescence in spermatozoa head which indicated the damage of membrane and late apoptotic cells. These changes reduced significantly in Vitamin E-treated groups though were not found to be comparable to control animals. All these changes were attributed to disrupted spermatogenesis that would interfere with sperm formation. Thus, the study infers that BPA affects the apoptosis process in the testis and epididymal sperm that would interfere with its function and contribute to infertility, whereas Vitamin E-supplemented dose has a protective effect towards these changes, indicating its role in improving male fertility.
Keywords	adults ; apoptosis ; biomarkers ; bisphenol A ; blood ; epididymis ; epoxides ; fluorescence ; histopathology ; laboratory animals ; male fertility ; males ; olive oil ; plasticizers ; plastics ; protective effect ; rats ; resins ; spermatozoa ; spermiogenesis ; staining ; testes ; testosterone ; vitamin E
Language	English
Dates of publication	2018-08
Size	p. 21635-21643.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-018-2229-2
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Article: Biogenic synthesis of CeO2 nanoparticles and its potential application as an efficient photocatalyst for the degradation of toxic amido black dye

Iqbal, Arshad / Ahmed, Arham S. / Ahmad, Nafees / Shafi, Adil / Ahamad, Tanveer / Khan, Mohammad Zain / Srivastava, Seema

Environmental nanotechnology, monitoring & management. 2021 Dec., v. 16

2021

Abstract: This work presents the green synthesis of nanoceria (CeO₂) by Phoenix dactylifera (Ajwa dates), Coriandrum sativum (Coriander), Ocimum tenuiflorum (Black tulsi), Camellia sinensis (green tea) and Hibiscus flower. The structural, morphological and optical ...

Abstract	This work presents the green synthesis of nanoceria (CeO₂) by Phoenix dactylifera (Ajwa dates), Coriandrum sativum (Coriander), Ocimum tenuiflorum (Black tulsi), Camellia sinensis (green tea) and Hibiscus flower. The structural, morphological and optical properties of green synthesized nanoceria were investigated by various analytical techniques such as XRD, FTIR, TEM, UV–visible, XPS and GC–MS. The formation of single phase CeO₂ NPs were confirmed by structural analysis and no impurity signal corresponding to metallic Ce was detected. The XPS measurements established that Ce ion has oxidation state + 4. The photocatalytic activity of various green synthesized nanoceria for the photodegradation of amido black dye was investigated and optimum degradation was obtained for Phoenix dactylifera synthesized nanoceria. Dye adsorption on the photocatalyst and the photogenerated charge carriers (e⁻ and h⁺) attributed to the photodegradation. Moreover trapping experiment was carried out to confirm the prime reactive species accountable for photodegradation. Recycling experiment was performed in order to determine the efficiency of the photocatalyst.
Keywords	Camellia sinensis ; Coriandrum sativum ; Hibiscus ; Ocimum tenuiflorum ; Phoenix dactylifera ; administrative management ; adsorption ; dyes ; flowers ; green tea ; nanoceria ; oxidation ; photocatalysis ; photocatalysts ; photolysis ; toxicity
Language	English
Dates of publication	2021-12
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2821777-9
ISSN	2215-1532
ISSN	2215-1532
DOI	10.1016/j.enmm.2021.100505
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Article ; Online: Prognostic value of National Early Warning Scores (NEWS2) and component physiology in hospitalised patients with COVID-19: a multicentre study.

Scott, Lauren J / Tavaré, Alison / Hill, Elizabeth M / Jordan, Lesley / Juniper, Mark / Srivastava, Seema / Redfern, Emma / Little, Hannah / Pullyblank, Anne

Emergency medicine journal : EMJ

2022 Volume 39, Issue 8, Page(s) 589–594

Abstract: Background: National Early Warning Scores (NEWS2) are used to detect all-cause deterioration. While studies have looked at NEWS2, the use of virtual consultation and remote monitoring of patients with COVID-19 mean there is a need to know which ... ...

Abstract	Background: National Early Warning Scores (NEWS2) are used to detect all-cause deterioration. While studies have looked at NEWS2, the use of virtual consultation and remote monitoring of patients with COVID-19 mean there is a need to know which physiological observations are important. Aim: To investigate the relationship between outcome and NEWS2, change in NEWS2 and component physiology in COVID-19 inpatients. Methods: A multi-centre retrospective study of electronically recorded, routinely collected physiological measurements between March and June 2020. First and maximum NEWS2, component scores and outcomes were recorded. Areas under the curve (AUCs) for 2-day, 7-day and 30-day mortality were calculated. Results: Of 1263 patients, 26% died, 7% were admitted to intensive care units (ICUs) before discharge and 67% were discharged without ICU. Of 1071 patients with initial NEWS2, most values were low: 50% NEWS2=0-2, 27% NEWS2=3-4, 14% NEWS2=5-6 and 9% NEWS2=7+. Maximum scores were: 14% NEWS2=0-2, 22% NEWS2=3-4, 17% NEWS2=5-6 and 47% NEWS2=7+. Higher first and maximum scores were predictive of mortality, ICU admission and longer length of stay. AUCs based on 2-day, 7-day, 30-day and any hospital mortality were 0.77 (95% CI 0.70 to 0.84), 0.70 (0.65 to 0.74), 0.65 (0.61 to 0.68) and 0.65 (0.61 to 0.68), respectively. The AUCs for 2-day mortality were 0.71 (0.65 to 0.77) for supplemental oxygen, 0.65 (0.56 to 0.73) oxygen saturation and 0.64 (0.56 to 0.73) respiratory rate. Conclusion: While respiratory parameters were most predictive, no individual parameter was as good as a full NEWS2, which is an acceptable predictor of short-term mortality in patients with COVID-19. This supports recommendation to use NEWS2 alongside clinical judgement to assess patients with COVID-19.
MeSH term(s)	COVID-19/diagnosis ; Early Warning Score ; Hospital Mortality ; Humans ; Prognosis ; Retrospective Studies
Language	English
Publishing date	2022-03-15
Publishing country	England
Document type	Journal Article ; Multicenter Study
ZDB-ID	2040124-3
ISSN	1472-0213 ; 1472-0205
ISSN (online)	1472-0213
ISSN	1472-0205
DOI	10.1136/emermed-2020-210624
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Article ; Online: Association between National Early Warning Scores in primary care and clinical outcomes: an observational study in UK primary and secondary care.

Scott, Lauren J / Redmond, Niamh M / Tavaré, Alison / Little, Hannah / Srivastava, Seema / Pullyblank, Anne

The British journal of general practice : the journal of the Royal College of General Practitioners

2020 Volume 70, Issue 695, Page(s) e374–e380

Abstract: Background: NHS England has mandated use of the National Early Warning Score (NEWS), more recently NEWS2, in acute settings, and suggested its use in primary care. However, there is reluctance from GPs to adopt NEWS/NEWS2.: Aim: To assess whether ... ...

Abstract	Background: NHS England has mandated use of the National Early Warning Score (NEWS), more recently NEWS2, in acute settings, and suggested its use in primary care. However, there is reluctance from GPs to adopt NEWS/NEWS2. Aim: To assess whether NEWS calculated at the point of GP referral into hospital is associated with outcomes in secondary care. Design and setting: An observational study using routinely collected data from primary and secondary care. Method: NEWS values were prospectively collected for 13 047 GP referrals into acute care between July 2017 and December 2018. NEWS values were examined and multivariate linear and logistic regression used to assess associations with process measures and clinical outcomes. Results: Higher NEWS values were associated with faster conveyance for patients travelling by ambulance, for example, median 94 minutes (interquartile range [IQR] 69-139) for NEWS ≥7; median 132 minutes, (IQR 84-236) for NEWS = 0 to 2); faster time from hospital arrival to medical review (54 minutes [IQR 25-114] for NEWS ≥7; 78 minutes [IQR 34-158] for NEWS = 0 to 2); as well as increased length of stay (5 days [IQR 2-11] versus 1 day [IQR 0-5]); intensive care unit admissions (2.0% versus 0.5%); sepsis diagnosis (11.7% versus 2.5%); and mortality, for example, 30-day mortality 12.0% versus 4.1% for NEWS ≥7 versus NEWS = 0 to 2, respectively. On average, for patients referred without a NEWS value (NEWS = NR), most clinical outcomes were comparable with patients with NEWS = 3 to 4, but ambulance conveyance time and time to medical review were comparable with patients with NEWS = 0 to 2. Conclusion: This study has demonstrated that higher NEWS values calculated at GP referral into hospital are associated with a faster medical review and poorer clinical outcomes.
MeSH term(s)	Early Warning Score ; England/epidemiology ; Humans ; Primary Health Care ; Retrospective Studies ; Secondary Care ; United Kingdom/epidemiology
Language	English
Publishing date	2020-05-28
Publishing country	England
Document type	Journal Article ; Observational Study
ZDB-ID	1043148-2
ISSN	1478-5242 ; 0035-8797 ; 0960-1643
ISSN (online)	1478-5242
ISSN	0035-8797 ; 0960-1643
DOI	10.3399/bjgp20X709337
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Article: Animal-derived medicinal products: community representatives' views of their use.

Harding, Sam / Williams, Leonie / Smith, Natalie / Soomro, Dur E Shahwar / Allawi, Sughra / Singh, Katie / Chowdhury, Monira / Deogan, Vardeep / Staples, Alison / Leung, Nixon / Srivastava, Seema / Adamali, Huzaifa / Barratt, Shaney L

Future healthcare journal

2023 Volume 10, Issue 3, Page(s) 291–295

Abstract: In the UK, there are a variety of religious or cultural beliefs and preferences that guide people in a range of lifestyle decisions. This qualitative study aimed to better understand the views of the public around prescribing animal-derived products, in ... ...

Abstract	In the UK, there are a variety of religious or cultural beliefs and preferences that guide people in a range of lifestyle decisions. This qualitative study aimed to better understand the views of the public around prescribing animal-derived products, in particular low-molecular-weight heparin (LMWH), from a potential patient perspective. A series of quality improvement focus groups with stakeholders were undertaken to understand perceptions and to evaluate and inform an established treatment pathway. Stakeholders discussed finding out about the porcine nature of LMWH asking 'Why don't they tell us?', suggesting that they 'shouldn't have to give out clues' about their personal preferences. Participants' thoughts about 'how' information be provided, by 'whom' and 'when' were gained. The stakeholders indicated that current practice is unacceptable for patients. They require greater knowledge and transparency regarding product components and recommend that healthcare professionals provide more dialogue and choice to patients.
Language	English
Publishing date	2023-10-19
Publishing country	England
Document type	Journal Article
ZDB-ID	3016427-8
ISSN	2514-6653 ; 2514-6645
ISSN (online)	2514-6653
ISSN	2514-6645
DOI	10.7861/fhj.2023-0005
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Article ; Online: A multicentre cross-sectional observational study to determine the effect of living with frailty on digital exclusion from video consultations: (Access-VIGIL).

Shorthose, Matthew F / Carter, Ben / Laidlaw, Jess / Watts, Nick / Wensley, Sue / Srivastava, Seema / Joughin, Andrea / Thorman, Emma / Mitchell, Colin / Evans, Rebekah / Braude, Philip

Journal of the American Medical Directors Association

2023 Volume 25, Issue 4, Page(s) 676–682

Abstract: Objectives: Many older people regularly access digital services, but many others are totally excluded. Age alone may not explain these discrepancies. As health care services offer more video consultations, we aimed to determine if living with frailty is ...

Abstract	Objectives: Many older people regularly access digital services, but many others are totally excluded. Age alone may not explain these discrepancies. As health care services offer more video consultations, we aimed to determine if living with frailty is a significant risk factor for digital exclusion in accessing video consultations, and if this changes if a person has a support network to help with access. Design: We undertook a muticenter cross-sectional survey across South West England. Setting and participants: Patients in primary care, hospital at home, and secondary care services were enrolled between February 21 and April 12, 2022. Methods: The primary outcome was complete digital exclusion defined as no individual access or network support access to video consultations. Secondary analysis looked at the person's digital exclusion when ignoring any network support. The association between frailty and outcomes was analyzed with logistic regression. In addition, older people's digital skills, motivation, and confidence were examined. Results: 255 patients were included in the analysis. The median age was 63 years (interquartile range 43-77) with 148 (57%) women. Complete digital exclusion was rare (5.1%). Only 1 of 155 who were not frail (Clinical Frailty Scale 1-3) experienced complete digital exclusion compared with 12 of 99 (10.7%) who were living with frailty (Clinical Frailty Scale 4-8). There was no association between frailty and complete digital exclusion. Frailty was associated with individual digital exclusion when no network support was available to assist. Conclusions and implications: When taking into account a person's support network, complete digital exclusion from video consultation was rare. When no support network was available, frailty was associated with individual digital exclusion. Health care services should ask about a person's support network to help people living with frailty access video consultations.
MeSH term(s)	Aged ; Female ; Humans ; Male ; Middle Aged ; Cross-Sectional Studies ; England ; Frailty/diagnosis ; Referral and Consultation ; Telemedicine ; Adult
Language	English
Publishing date	2023-10-16
Publishing country	United States
Document type	Observational Study ; Multicenter Study ; Journal Article
ZDB-ID	2171030-2
ISSN	1538-9375 ; 1525-8610
ISSN (online)	1538-9375
ISSN	1525-8610
DOI	10.1016/j.jamda.2023.08.028
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