Article: Treatment of dialysis membranes for simultaneous dialysis and concentration.
1985 Volume 145, Issue 2, Page(s) 343–350
Abstract: Dialysis membranes used for simultaneous dialysis-concentration required pretreatment to remove uv-absorbing compounds leached from the membranes and to reduce the absorption of protein to the membranes. This was accomplished with sodium carbonate and ... ...
Abstract | Dialysis membranes used for simultaneous dialysis-concentration required pretreatment to remove uv-absorbing compounds leached from the membranes and to reduce the absorption of protein to the membranes. This was accomplished with sodium carbonate and ethanol or with "sulfur-removal solutions." Protein determinations were made with a micro-Bradford protein reaction and with uv absorbance at 280 nm. Soluble membrane components contributed to aberrant uv spectra and altered the ratio of 280/260-nm absorbance. Simultaneous dialysis and concentration in the micro protein dialyzer-concentrator apparatus, combining aspects of thin-layer dialysis and ultrafiltration, resulted in rapid removal of salts from the protein solutions. Prior treatment of membranes reduced uncertainties in retentate recoveries, eliminated uv-absorbing components of membranes, and improved recoveries of protein. |
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MeSH term(s) | Acetates ; Azides ; Carbonates ; Dialysis ; Ethanol ; Membranes, Artificial ; Proteins/isolation & purification ; Serum Albumin, Bovine/isolation & purification ; Sodium Azide ; Spectrophotometry, Ultraviolet |
Chemical Substances | Acetates ; Azides ; Carbonates ; Membranes, Artificial ; Proteins ; Serum Albumin, Bovine (27432CM55Q) ; Ethanol (3K9958V90M) ; sodium carbonate (45P3261C7T) ; Sodium Azide (968JJ8C9DV) ; ammonium acetate (RRE756S6Q2) |
Language | English |
Publishing date | 1985-03 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 1110-1 |
ISSN | 1096-0309 ; 0003-2697 |
ISSN (online) | 1096-0309 |
ISSN | 0003-2697 |
DOI | 10.1016/0003-2697(85)90372-0 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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