LIVIVO - Suchergebnisse -

Suchergebnis

Treffer 1 - 10 von insgesamt 95

Suchoptionen

Artikel: Bovine Immunity and Vitamin D3: An Emerging Association in Johne’s Disease

Wherry, Taylor L. T. / Stabel, Judith R.

Microorganisms. 2022 Sept. 18, v. 10, no. 9

2022

Abstract: Mycobacterium avium subspecies paratuberculosis (MAP) is an environmentally hardy pathogen of ruminants that plagues the dairy industry. Hallmark clinical symptoms include granulomatous enteritis, watery diarrhea, and significant loss of body condition. ... ...

Abstract	Mycobacterium avium subspecies paratuberculosis (MAP) is an environmentally hardy pathogen of ruminants that plagues the dairy industry. Hallmark clinical symptoms include granulomatous enteritis, watery diarrhea, and significant loss of body condition. Transition from subclinical to clinical infection is a dynamic process led by MAP which resides in host macrophages. Clinical stage disease is accompanied by dysfunctional immune responses and a reduction in circulating vitamin D₃. The immunomodulatory role of vitamin D₃ in infectious disease has been well established in humans, particularly in Mycobacterium tuberculosis infection. However, significant species differences exist between the immune system of humans and bovines, including effects induced by vitamin D₃. This fact highlights the need for continued study of the relationship between vitamin D₃ and bovine immunity, especially during different stages of paratuberculosis.
Schlagwörter	Mycobacterium avium subsp. paratuberculosis ; Mycobacterium tuberculosis ; body condition ; cattle ; cholecalciferol ; dairy industry ; diarrhea ; enteritis ; immunity ; macrophages ; paratuberculosis ; pathogens
Sprache	Englisch
Erscheinungsverlauf	2022-0918
Erscheinungsort	Multidisciplinary Digital Publishing Institute
Dokumenttyp	Artikel
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10091865
Datenquelle	NAL Katalog (AGRICOLA)

Zusatzmaterialien

Über subito bestellen

Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Improved DNA Amplification of the Hallmark IS

Bannantine, John P / Stabel, Judith R / Bayles, Darrell O / Biet, Franck

Applied and environmental microbiology

2023 Band 89, Heft 2, Seite(n) e0168222

Abstract: Amplification of the ... ...

Abstract	Amplification of the IS
Mesh-Begriff(e)	Female ; Cattle ; Sheep ; Animals ; Mycobacterium avium subsp. paratuberculosis/genetics ; Paratuberculosis/diagnosis ; Paratuberculosis/genetics ; Paratuberculosis/microbiology ; Real-Time Polymerase Chain Reaction ; DNA Transposable Elements ; DNA, Bacterial/genetics ; DNA, Bacterial/analysis ; Feces/microbiology ; Cattle Diseases/diagnosis ; Cattle Diseases/microbiology
Chemische Substanzen	DNA Transposable Elements ; DNA, Bacterial
Sprache	Englisch
Erscheinungsdatum	2023-01-31
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/aem.01682-22
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Zs.B 201: Hefte anzeigen

Standort:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Details ▾
- Im ZB MED-Bestand
- Kostenpflichtig bestellen

Artikel: Effects of 1,25-Dihydroxyvitamin D

Wherry, Taylor L T / Mooyottu, Shankumar / Stabel, Judith R

Frontiers in veterinary science

2022 Band 9, Seite(n) 830144

Abstract: The role of vitamin ... ...

Abstract	The role of vitamin D
Sprache	Englisch
Erscheinungsdatum	2022-02-08
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2834243-4
ISSN	2297-1769
ISSN	2297-1769
DOI	10.3389/fvets.2022.830144
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel: Immunological Evaluation of Goats Immunized with a Commercial Vaccine against Johne's Disease.

Bannantine, John P / Stabel, Judith R / Kapur, Vivek

Vaccines

2022 Band 10, Heft 4

Abstract: Johne's disease affects ruminants causing an economic burden to dairy, meat and wool industries. Vaccination ... ...

Abstract	Johne's disease affects ruminants causing an economic burden to dairy, meat and wool industries. Vaccination against
Sprache	Englisch
Erscheinungsdatum	2022-03-26
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines10040518
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel: Bovine Immunity and Vitamin D

Wherry, Taylor L T / Stabel, Judith R

Microorganisms

2022 Band 10, Heft 9

Abstract: Mycobacterium ... ...

Abstract	Mycobacterium avium
Sprache	Englisch
Erscheinungsdatum	2022-09-18
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article ; Review
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10091865
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Comparison of methods to isolate peripheral blood mononuclear cells from cattle blood.

Stabel, Judith R / Wherry, Taylor L T

Journal of immunological methods

2022 Band 512, Seite(n) 113407

Abstract: Peripheral blood mononuclear cells (PBMCs) are critical for assessment of host immune responses to infectious disease. The isolation of PBMCs from whole blood is a laborious process involving density gradients and multiple centrifugation steps. In the ... ...

Abstract	Peripheral blood mononuclear cells (PBMCs) are critical for assessment of host immune responses to infectious disease. The isolation of PBMCs from whole blood is a laborious process involving density gradients and multiple centrifugation steps. In the present study we compared a more traditional method of PBMC isolation used in our laboratory to two novel methods of cell isolation for efficiency, cell viability, and enumeration of cell subsets. Our laboratory method uses Histopaque-1077 density gradient in standard conical tubes and this was compared with isolation of cells using SepMate™ tubes, a novel conical tube containing an insert to separate the density gradient. Multiple experiments were performed to optimize the SepMate™ tubes for use with cattle blood. A final experiment was conducted to compare traditional methodology, the optimized SepMate™ method with a more novel method using cell preparation tubes (CPT-10 vacutainers containing density gradient). Results demonstrated that optimization of the SepMate™ tube methodology was necessary, including dilution of blood and addition of centrifugation steps to reduce platelet contamination. The CPT-10 tubes worked well but cell recovery was lower compared to other methods. Both of the newer methods were comparable to a modified version of our traditional laboratory method of PBMC isolation in terms of numbers of recovered viable cells and the frequency of immune cell subsets. Additionally, efficiency was improved, particularly with the SepMate™ tube method, resulting in reduced time in the laboratory as well as reduced usage of plasticware.
Mesh-Begriff(e)	Cattle ; Animals ; Leukocytes, Mononuclear ; Cell Separation/methods ; Cell Survival ; Centrifugation, Density Gradient/methods ; Centrifugation
Sprache	Englisch
Erscheinungsdatum	2022-12-15
Erscheinungsland	Netherlands
Dokumenttyp	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	120142-6
ISSN	1872-7905 ; 0022-1759
ISSN (online)	1872-7905
ISSN	0022-1759
DOI	10.1016/j.jim.2022.113407
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Zs.A 795: Hefte anzeigen

Standort:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Details ▾

Artikel ; Online: Reduced tissue colonization of Mycobacterium avium subsp. paratuberculosis in neonatal calves vaccinated with a cocktail of recombinant proteins

Stabel, Judith R. / Bannatine, J. P.

Vaccine. 2021 May 27, v. 39, no. 23, p. 3131-3140

2021 , Seite(n) 3131–3140

Abstract: An increasing prevalence of paratuberculosis supports the need for new efficacious vaccines as an essential management tool. Two separate studies were performed in neonatal calves to evaluate the effectiveness of pooled recombinant Mycobacterium avium ... ...

Abstract	An increasing prevalence of paratuberculosis supports the need for new efficacious vaccines as an essential management tool. Two separate studies were performed in neonatal calves to evaluate the effectiveness of pooled recombinant Mycobacterium avium subsp. paratuberculosis (MAP) proteins (MAP1087, MAP1204, MAP1272c, MAP2077c) as a potential vaccine. In the first study vaccinated calves were immunized with 400 µg protein cocktail per dose, whereas the second study compared doses of 400 µg and 800 µg of protein cocktail, followed by challenge with live MAP for both vaccinated and nonvaccinated control calves 28 days post-vaccination. At the end of 12 months, tissue colonization with MAP was significantly reduced for the vaccinated calves compared to control animals. A higher dose of vaccine improved protection, with further reductions of MAP burden. Antigen-specific IFN-γ responses and serum antibody responses were similar regardless of vaccination, indicating exposure to MAP invoked conventional host immune responses. Host immunity differed due to vaccination, resulting in increased percentages of CD4+ T cells and B cells after stimulation of PBMCs with antigen. Interestingly, gene expression in PBMCs was similar for both control and vaccinated calves except for significant increases in IFN-γ, IL-12, and IL-17 expression observed in vaccinated calves. Vaccination with a cocktail of immunogenic recombinant MAP proteins was efficacious in reducing the level of infection and fecal shedding of neonatal calves and may be a potential tool for curtailing the spread of Johne’s disease.
Schlagwörter	Mycobacterium avium subsp. paratuberculosis ; Vaccine ; Recombinant proteins ; Immunity
Sprache	Englisch
Erscheinungsverlauf	2021-0527
Umfang	p. 3131-3140
Erscheinungsort	Elsevier Ltd
Dokumenttyp	Artikel ; Online
Anmerkung	Resource is Open Access ; Consyn License Information
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2021.04.051
Datenquelle	NAL Katalog (AGRICOLA)

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Zs.A 1930: Hefte anzeigen			Standort: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 458: Hefte anzeigen

Über subito bestellen

Details ▾

Artikel: Stage of infection with

Wherry, Taylor L T / Heggen, Mark / Shircliff, Adrienne L / Mooyottu, Shankumar / Stabel, Judith R

Frontiers in veterinary science

2023 Band 10, Seite(n) 1117591

Abstract: Introduction: Macrophages are the preferential target of : Methods: This study aimed to characterize expression of early and late endosomal markers Rab5 and Rab7, respectively, within CD68+ macrophages in frozen mid-ileum sections harvested from cows ...

Abstract	Introduction: Macrophages are the preferential target of Methods: This study aimed to characterize expression of early and late endosomal markers Rab5 and Rab7, respectively, within CD68+ macrophages in frozen mid-ileum sections harvested from cows at different stages of natural paratuberculosis infection. Additionally, factors of vitamin D Results and discussion: Cows with clinical paratuberculosis had significantly greater macrophage and MAP burden overall, as well as intracellular MAP. Total expression of Rab5 within macrophages was reduced in clinical cows; however, Rab5 and MAP colocalization was significantly greater in this group. Intracellular Rab7 colocalization with MAP was not detected in subclinical or Johne's Disease negative (JD-) control cows but was present in clinical cows. Additionally, macrophage CYP27B1 expression was significantly reduced in clinical cows. Taken together, the results from this study show disparate patterns of expression for key mediators in intracellular MAP trafficking and vitamin D metabolism for cows at different stages of paratuberculosis.
Sprache	Englisch
Erscheinungsdatum	2023-02-03
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2834243-4
ISSN	2297-1769
ISSN	2297-1769
DOI	10.3389/fvets.2023.1117591
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Comparison of Sheep, Goats, and Calves as Infection Models for Mycobacterium avium subsp. paratuberculosis.

Stabel, Judith R / Bannantine, John P / Hostetter, Jesse M

Veterinary immunology and immunopathology

2020 Band 225, Seite(n) 110060

Abstract: Animal infection models to study Mycobacterium avium subsp. paratuberculosis (MAP) infection are useful for evaluating the efficacy of vaccines and other therapeutics for the prevention or treatment of infection. The goal of the present study was to ... ...

Abstract	Animal infection models to study Mycobacterium avium subsp. paratuberculosis (MAP) infection are useful for evaluating the efficacy of vaccines and other therapeutics for the prevention or treatment of infection. The goal of the present study was to compare smaller ruminants, sheep and goats, with calves as infection models. Neonatal sheep, goats, and calves (n = 4) received 10
Mesh-Begriff(e)	Animals ; Animals, Newborn ; Antigens, Bacterial/pharmacology ; Cattle/microbiology ; Cytokines/immunology ; Disease Models, Animal ; Feces/microbiology ; Goats/microbiology ; Interferon-gamma/immunology ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/immunology ; Mycobacterium avium subsp. paratuberculosis ; Paratuberculosis/immunology ; Paratuberculosis/microbiology ; Sheep/microbiology
Chemische Substanzen	Antigens, Bacterial ; Cytokines ; Interferon-gamma (82115-62-6)
Sprache	Englisch
Erscheinungsdatum	2020-05-05
Erscheinungsland	Netherlands
Dokumenttyp	Comparative Study ; Journal Article
ZDB-ID	754160-0
ISSN	1873-2534 ; 0165-2427
ISSN (online)	1873-2534
ISSN	0165-2427
DOI	10.1016/j.vetimm.2020.110060
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Zs.A 2177: Hefte anzeigen

Standort:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Details ▾

Artikel ; Online: Vitamin D3 alters macrophage phenotype and endosomal trafficking markers in dairy cattle naturally infected with Mycobacterium avium subsp. paratuberculosis.

Wherry, Taylor L T / Dassanayake, Rohana P / Bannantine, John P / Mooyottu, Shankumar / Stabel, Judith R

Frontiers in cellular and infection microbiology

2022 Band 12, Seite(n) 1021657

Abstract: Macrophages are important host defense cells in ruminant paratuberculosis (Johne's Disease; JD), a chronic enteritis caused by ... Mycobacterium avium ... subsp. ... paratuberculosis ... (MAP). Classical macrophage functions of pathogen trafficking, ... ...

Abstract	Macrophages are important host defense cells in ruminant paratuberculosis (Johne's Disease; JD), a chronic enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP). Classical macrophage functions of pathogen trafficking, degradation, and antigen presentation are interrupted in mycobacterial infection. Immunologic stimulation by 25-hydroxyvitamin D<sub>3</sub> (25(OH)D<sub>3</sub>) and 1,25-dihydroxyvitamin D<sub>3</sub> (1,25(OH)<sub>2</sub>D<sub>3</sub>) enhances bovine macrophage function. The present study aimed to investigate the role of vitamin D<sub>3</sub> on macrophage phenotype and endosomal trafficking of MAP in monocyte-derived macrophages (MDMs) cultured from JD-, JD+ subclinical, and JD+ clinically infected cattle. MDMs were pre-treated 100 ng/ml 25(OH)D<sub>3</sub> or 4 ng/ml 1,25(OH)<sub>2</sub>D<sub>3</sub> and incubated 24 hrs with MAP at 10:1 multiplicity of infection (MOI). In vitro MAP infection upregulated pro-inflammatory (M1) CD80 and downregulated resolution/repair (M2) CD163. Vitamin D<sub>3</sub> generally decreased CD80 and increased CD163 expression. Furthermore, early endosomal marker Rab5 was upregulated 140× across all stages of paratuberculosis infection following in vitro MAP infection; however, Rab5 was reduced in MAP-activated MDMs from JD+ subclinical and JD+ clinical cows compared to healthy controls. Rab7 expression decreased in control and clinical cows following MDM infection with MAP. Both forms of vitamin D<sub>3</sub> reduced Rab5 expression in infected MDMs from JD- control cows, while 1,25(OH)<sub>2</sub>D<sub>3</sub> decreased Rab7 expression in JD- and JD+ subclinical animals regardless of MAP infection in vitro. Vitamin D<sub>3</sub> promoted phagocytosis in MDMs from JD- and JD+ clinical cows treated with either vitamin D<sub>3</sub> analog. Results from this study show exogenous vitamin D<sub>3</sub> influences macrophage M1/M2 polarization and Rab GTPase expression within MDM culture.
Mesh-Begriff(e)	Female ; Cattle ; Animals ; Mycobacterium avium subsp. paratuberculosis ; Paratuberculosis/microbiology ; Cholecalciferol/pharmacology ; Cattle Diseases ; Macrophages/microbiology ; Phenotype ; rab GTP-Binding Proteins/genetics
Chemische Substanzen	Cholecalciferol (1C6V77QF41) ; rab GTP-Binding Proteins (EC 3.6.5.2)
Sprache	Englisch
Erscheinungsdatum	2022-10-05
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2022.1021657
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Zum Seitenanfang

Ihre letzten Suchen

Zusatzmaterialien

Kategorien

Über subito bestellen

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Zusatzmaterialien

Kategorien

Über subito bestellen

Zusatzmaterialien

Kategorien

Über subito bestellen

Zusatzmaterialien

Kategorien

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Zusatzmaterialien

Kategorien

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Zusatzmaterialien

Kategorien

Über subito bestellen