Article: Integrated bioassays in microfluidic devices: botulinum toxin assays.
Journal of biomolecular screening
2005 Volume 10, Issue 8, Page(s) 788–794
Abstract: A microfluidic assay was developed for screening botulinum neurotoxin serotype A (BoNT-A) by using a fluorescent resonance energy transfer (FRET) assay. Molded silicone microdevices with integral valves, pumps, and reagent reservoirs were designed and ... ...
Abstract | A microfluidic assay was developed for screening botulinum neurotoxin serotype A (BoNT-A) by using a fluorescent resonance energy transfer (FRET) assay. Molded silicone microdevices with integral valves, pumps, and reagent reservoirs were designed and fabricated. Electrical and pneumatic control hardware were constructed, and software was written to automate the assay protocol and data acquisition. Detection was accomplished by fluorescence microscopy. The system was validated with a peptide inhibitor, running 2 parallel assays, as a feasibility demonstration. The small footprint of each bioreactor cell (0.5 cm2) and scalable fluidic architecture enabled many parallel assays on a single chip. The chip is programmable to run a dilution series in each lane, generating concentration-response data for multiple inhibitors. The assay results showed good agreement with the corresponding experiments done at a macroscale level. Although the system has been developed for BoNT-A screening, a wide variety of assays can be performed on the microfluidic chip with little or no modification. |
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MeSH term(s) | Biological Assay/instrumentation ; Biological Assay/methods ; Botulinum Toxins, Type A/analysis ; Feasibility Studies ; Microchemistry/instrumentation ; Microfluidics/instrumentation ; Miniaturization |
Chemical Substances | Botulinum Toxins, Type A (EC 3.4.24.69) |
Language | English |
Publishing date | 2005-12 |
Publishing country | United States |
Document type | Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Validation Studies |
ZDB-ID | 1433680-7 |
ISSN | 1552-454X ; 1087-0571 |
ISSN (online) | 1552-454X |
ISSN | 1087-0571 |
DOI | 10.1177/1087057105278927 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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