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  1. Article: Caspofungin resistant disseminated candidiasis in a 7-year-old girl with T cell lymphoma: a case report.

    Schmalz, Michael / Joysula, Manasa / Staddon, Jack H / Feinberg, Arthur

    Translational pediatrics

    2018  Volume 7, Issue 1, Page(s) 63–66

    Abstract: Immunocompromised patients are at increased risk of disseminated candidiasis. Guidelines for the treatment of invasive candidiasis were last published in 2009, but resistance to the recommended treatment has recently been described in the literature. ... ...

    Abstract Immunocompromised patients are at increased risk of disseminated candidiasis. Guidelines for the treatment of invasive candidiasis were last published in 2009, but resistance to the recommended treatment has recently been described in the literature. Here we present the case of an immunocompromised child with T-cell lymphoma who died secondary to disseminated candidiasis despite prolonged antifungal therapy. Awareness of the increasing resistance patterns of Candida when caring for immunocompromised patients, especially pediatric patients, may improve treatment and create better patient outcomes.
    Language English
    Publishing date 2018-01-26
    Publishing country China
    Document type Case Reports
    ZDB-ID 2901309-4
    ISSN 2224-4344 ; 2224-4344 ; 2224-4336
    ISSN (online) 2224-4344
    ISSN 2224-4344 ; 2224-4336
    DOI 10.21037/tp.2017.06.02
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Specificity determinants of conjugative DNA processing in the Enterococcus faecalis plasmid pCF10 and the Lactococcus lactis plasmid pRS01.

    Chen, Yuqing / Staddon, Jack H / Dunny, Gary M

    Molecular microbiology

    2007  Volume 63, Issue 5, Page(s) 1549–1564

    Abstract: The DNA-processing region of the Enterococcus faecalis pheromone-responsive plasmid pCF10 is highly similar to that of the otherwise unrelated plasmid pRS01 from Lactococcus lactis. A transfer-proficient pRS01 derivative was unable to mobilize plasmids ... ...

    Abstract The DNA-processing region of the Enterococcus faecalis pheromone-responsive plasmid pCF10 is highly similar to that of the otherwise unrelated plasmid pRS01 from Lactococcus lactis. A transfer-proficient pRS01 derivative was unable to mobilize plasmids containing the pCF10 origin of transfer, oriT. In contrast, pRS01 oriT-containing plasmids could be mobilized by pCF10 at a low frequency. Relaxases PcfG and LtrB were both capable of binding to single-stranded oriT DNAs; LtrB was highly specific for its cognate oriT, whereas PcfG could recognize both pCF10 and pRS01 oriT. However, pcfG was unable to complement an ltrB insertion mutation. Genetic analysis showed that pcfF of pCF10 and ltrF of pRS01 are also essential for plasmid transfer. Purified PcfF and LtrF possess double-stranded DNA binding activities for the inverted repeat within either oriT sequence. PcfG and LtrB were recruited into their cognate F-oriT DNA complex through direct interactions with their cognate accessory protein. PcfG also could interact with LtrF when pCF10 oriT was present. In vivo cross-complementation analysis showed that ltrF partially restored the pCF10DeltapcfF mutant transfer ability when provided in trans, whereas pcfF failed to complement an ltrF mutation. Specificity of conjugative DNA processing in these plasmids involves both DNA-protein and protein-protein interactions.
    MeSH term(s) Bacterial Proteins/metabolism ; Conjugation, Genetic ; DNA Transposable Elements ; DNA, Bacterial/genetics ; DNA, Bacterial/metabolism ; DNA, Single-Stranded/metabolism ; Electrophoretic Mobility Shift Assay ; Enterococcus faecalis/genetics ; Gene Deletion ; Genetic Complementation Test ; Lactococcus lactis/genetics ; Molecular Sequence Data ; Mutagenesis, Insertional ; Plasmids/genetics ; Protein Binding ; Repetitive Sequences, Nucleic Acid ; Replication Origin/physiology
    Chemical Substances Bacterial Proteins ; DNA Transposable Elements ; DNA, Bacterial ; DNA, Single-Stranded ; LtrB protein, Lactococcus lactis
    Language English
    Publishing date 2007-03
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 619315-8
    ISSN 1365-2958 ; 0950-382X
    ISSN (online) 1365-2958
    ISSN 0950-382X
    DOI 10.1111/j.1365-2958.2007.05610.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2502: Show issues Location:
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  3. Article ; Online: Pegasparaginase treatment alters thrombin generation by modulating the protein C and S system in acute lymphoblastic leukaemia/lymphoma.

    Staddon, Jack H / Smock, Kristi J / Schiffman, Joshua D / Fluchel, Mark N / Engel, Michael E / Weyrich, Andrew S / Campbell, Robert A

    Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis

    2015  Volume 26, Issue 7, Page(s) 840–843

    Abstract: Paediatric patients with acute lymphoblastic leukaemia/lymphoma treated with pegasparaginase are at an increased risk of thrombosis. We evaluated changes in thrombin generation in the presence and absence of thrombomodulin using paired plasma samples ... ...

    Abstract Paediatric patients with acute lymphoblastic leukaemia/lymphoma treated with pegasparaginase are at an increased risk of thrombosis. We evaluated changes in thrombin generation in the presence and absence of thrombomodulin using paired plasma samples collected from paediatric patients treated with pegasparaginase. Postpegasparaginase samples were significantly less sensitive to reductions in thrombin generation in the presence of thrombomodulin compared with prepegasparaginase, suggesting reduced protein C and S activity. This corresponded to a significant decrease in protein C and protein S antigen. Alterations in the protein C and S pathway may contribute to the increased risk of thrombosis in patients treated with pegasparaginase.
    MeSH term(s) Antineoplastic Agents/administration & dosage ; Antineoplastic Agents/therapeutic use ; Asparaginase/administration & dosage ; Asparaginase/therapeutic use ; Child ; Female ; Humans ; Male ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy ; Protein C/metabolism ; Protein S/metabolism ; Thrombin/drug effects
    Chemical Substances Antineoplastic Agents ; Protein C ; Protein S ; Thrombin (EC 3.4.21.5) ; Asparaginase (EC 3.5.1.1)
    Language English
    Publishing date 2015-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 1033551-1
    ISSN 1473-5733 ; 0957-5235
    ISSN (online) 1473-5733
    ISSN 0957-5235
    DOI 10.1097/MBC.0000000000000356
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2920: Show issues Location:
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  4. Article: Specificity determinants of conjugative DNA processing in the Enterococcus faecalis plasmid pCF10 and the Lactococcus lactis plasmid pRS01

    Chen, Yuqing / Staddon, Jack H / Dunny, Gary M

    Molecular microbiology. 2007 Mar., v. 63, no. 5

    2007  

    Abstract: The DNA-processing region of the Enterococcus faecalis pheromone-responsive plasmid pCF10 is highly similar to that of the otherwise unrelated plasmid pRS01 from Lactococcus lactis. A transfer-proficient pRS01 derivative was unable to mobilize plasmids ... ...

    Abstract The DNA-processing region of the Enterococcus faecalis pheromone-responsive plasmid pCF10 is highly similar to that of the otherwise unrelated plasmid pRS01 from Lactococcus lactis. A transfer-proficient pRS01 derivative was unable to mobilize plasmids containing the pCF10 origin of transfer, oriT. In contrast, pRS01 oriT-containing plasmids could be mobilized by pCF10 at a low frequency. Relaxases PcfG and LtrB were both capable of binding to single-stranded oriT DNAs; LtrB was highly specific for its cognate oriT, whereas PcfG could recognize both pCF10 and pRS01 oriT. However, pcfG was unable to complement an ltrB insertion mutation. Genetic analysis showed that pcfF of pCF10 and ltrF of pRS01 are also essential for plasmid transfer. Purified PcfF and LtrF possess double-stranded DNA binding activities for the inverted repeat within either oriT sequence. PcfG and LtrB were recruited into their cognate F-oriT DNA complex through direct interactions with their cognate accessory protein. PcfG also could interact with LtrF when pCF10 oriT was present. In vivo cross-complementation analysis showed that ltrF partially restored the pCF10ΔpcfF mutant transfer ability when provided in trans, whereas pcfF failed to complement an ltrF mutation. Specificity of conjugative DNA processing in these plasmids involves both DNA-protein and protein-protein interactions.
    Language English
    Dates of publication 2007-03
    Size p. 1549-1564.
    Publisher Blackwell Publishing Ltd
    Publishing place Oxford, UK
    Document type Article
    ZDB-ID 619315-8
    ISSN 1365-2958 ; 0950-382X
    ISSN (online) 1365-2958
    ISSN 0950-382X
    DOI 10.1111/j.1365-2958.2007.05610.x
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Conserved target for group II intron insertion in relaxase genes of conjugative elements of gram-positive bacteria.

    Staddon, Jack H / Bryan, Edward M / Manias, Dawn A / Dunny, Gary M

    Journal of bacteriology

    2004  Volume 186, Issue 8, Page(s) 2393–2401

    Abstract: The lactococcal group II intron Ll.ltrB interrupts the ltrB relaxase gene within a region that encodes a conserved functional domain. Nucleotides essential for the homing of Ll.ltrB into an intronless version of ltrB are found exclusively at positions ... ...

    Abstract The lactococcal group II intron Ll.ltrB interrupts the ltrB relaxase gene within a region that encodes a conserved functional domain. Nucleotides essential for the homing of Ll.ltrB into an intronless version of ltrB are found exclusively at positions required to encode amino acids broadly conserved in a family of relaxase proteins of gram-positive bacteria. Two of these relaxase genes, pcfG from the enterococcal plasmid pCF10 and the ORF4 gene in the streptococcal conjugative transposon Tn5252, were shown to support Ll.ltrB insertion into the conserved motif at precisely the site predicted by sequence homology with ltrB. Insertion occurred through a mechanism indistinguishable from retrohoming. Splicing and retention of conjugative function was demonstrated for pCF10 derivatives containing intron insertions. Ll.ltrB targeting of a conserved motif of a conjugative element suggests a mechanism for group II intron dispersal among bacteria. Additional support for this mechanism comes from sequence analysis of the insertion sites of the E.c.I4 family of bacterial group II introns.
    MeSH term(s) Amino Acid Sequence ; Bacterial Proteins/genetics ; Conjugation, Genetic ; DNA Nucleotidyltransferases/genetics ; DNA Transposable Elements/genetics ; Gene Targeting ; Genes, Bacterial ; Gram-Positive Bacteria/genetics ; Introns ; Molecular Sequence Data ; Mutagenesis, Insertional ; Open Reading Frames ; Plasmids/genetics ; Protein Structure, Tertiary/genetics ; Sequence Alignment
    Chemical Substances Bacterial Proteins ; DNA Transposable Elements ; LtrB protein, Lactococcus lactis ; DNA Nucleotidyltransferases (EC 2.7.7.-) ; DNA relaxase (EC 2.7.7.-)
    Language English
    Publishing date 2004-02-17
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/JB.186.8.2393-2401.2004
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.50: Show issues Location:
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  6. Article: Genetic characterization of the conjugative DNA processing system of enterococcal plasmid pCF10.

    Staddon, Jack H / Bryan, Edward M / Manias, Dawn A / Chen, Yuqing / Dunny, Gary M

    Plasmid

    2006  Volume 56, Issue 2, Page(s) 102–111

    Abstract: Conjugation is a major contributor to lateral gene transfer in bacteria, and pheromone-inducible conjugation systems in Enterococcus faecalis play an important role in the dissemination of antibiotic resistance and virulence in enterococci and related ... ...

    Abstract Conjugation is a major contributor to lateral gene transfer in bacteria, and pheromone-inducible conjugation systems in Enterococcus faecalis play an important role in the dissemination of antibiotic resistance and virulence in enterococci and related bacteria. We have genetically dissected the determinants of DNA processing of the enterococcal conjugative plasmid pCF10. Insertional inactivation of a predicted relaxase gene pcfG, via insertion of a splicing-deficient group II intron, severely reduced pCF10 transfer. Restoration of intron splicing ability by genetic complementation restored conjugation. The pCF10 origin of transfer (oriT) was localized to a 40-nucleotide sequence within a non-coding region with sequence similarity to origins of transfer of several other plasmids in gram positive bacteria. Deletion of the oriT reduced pCF10 transfer by more than five orders of magnitude without affecting pCF10-dependent mobilization of co-resident oriT-containing plasmids. Although the host range for pCF10 replication is limited to enterococci, we found that the pCF10 conjugation system promotes mobilization of oriT-containing plasmids to multiple bacterial genera. Therefore, this transfer system may have applications for gene delivery to a variety of poorly-transformed bacteria.
    MeSH term(s) Base Sequence ; Chromosome Mapping ; Computational Biology ; Conjugation, Genetic/genetics ; DNA Primers ; DNA, Bacterial/genetics ; Electroporation ; Enterococcus faecalis/genetics ; Gene Transfer Techniques ; Molecular Sequence Data ; Plasmids/genetics ; Polymerase Chain Reaction ; Replication Origin/genetics ; Sequence Alignment ; Sequence Analysis, DNA
    Chemical Substances DNA Primers ; DNA, Bacterial
    Language English
    Publishing date 2006-09
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 282384-6
    ISSN 1095-9890 ; 0147-619X
    ISSN (online) 1095-9890
    ISSN 0147-619X
    DOI 10.1016/j.plasmid.2006.05.001
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1736: Show issues Location:
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  7. Article: Characterization of the pheromone response of the Enterococcus faecalis conjugative plasmid pCF10: complete sequence and comparative analysis of the transcriptional and phenotypic responses of pCF10-containing cells to pheromone induction.

    Hirt, Helmut / Manias, Dawn A / Bryan, Edward M / Klein, Joanna R / Marklund, Jesper K / Staddon, Jack H / Paustian, Michael L / Kapur, Vivek / Dunny, Gary M

    Journal of bacteriology

    2005  Volume 187, Issue 3, Page(s) 1044–1054

    Abstract: The sex pheromone plasmids in Enterococcus faecalis are one of the most efficient conjugative plasmid transfer systems known in bacteria. Plasmid transfer rates can reach or exceed 10(-1) transconjugants per donor in vivo and under laboratory conditions. ...

    Abstract The sex pheromone plasmids in Enterococcus faecalis are one of the most efficient conjugative plasmid transfer systems known in bacteria. Plasmid transfer rates can reach or exceed 10(-1) transconjugants per donor in vivo and under laboratory conditions. We report the completion of the DNA sequence of plasmid pCF10 and the analysis of the transcription profile of plasmid genes, relative to conjugative transfer ability following pheromone induction. These experiments employed a mini-microarray containing all 57 open reading frames of pCF10 and a set of selected chromosomal genes. A clear peak of transcription activity was observed 30 to 60 min after pheromone addition, with transcription subsiding 2 h after pheromone induction. The transcript activity correlated with the ability of donor cells to transfer pCF10 to recipient cells. Remarkably, aggregation substance (Asc10, encoded by the prgB gene) was present on the cell surface for a long period of time after pheromone-induced transcription of prgB and plasmid transfer ability had ceased. This observation could have relevance for the virulence of E. faecalis.
    MeSH term(s) Chromosome Mapping ; DNA, Bacterial/genetics ; Enterococcus faecalis/genetics ; Phenotype ; Pheromones/genetics ; Pheromones/physiology ; Phylogeny ; Plasmids/genetics ; Polymerase Chain Reaction ; Transcription, Genetic/genetics
    Chemical Substances DNA, Bacterial ; Pheromones
    Language English
    Publishing date 2005-02
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/JB.187.3.1044-1054.2005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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