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  1. Article ; Online: Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level.

    Masters-Clark, Emily / Clark, Amelia J / Stanley, Claire E

    Journal of visualized experiments : JoVE

    2022  , Issue 184

    Abstract: Filamentous fungi are successful inhabitants of soil and play a major role in soil ecosystems, such as in the decomposition of organic and inorganic matter, as well as regulation of nutrient levels. There they also find numerous opportunities to interact ...

    Abstract Filamentous fungi are successful inhabitants of soil and play a major role in soil ecosystems, such as in the decomposition of organic and inorganic matter, as well as regulation of nutrient levels. There they also find numerous opportunities to interact with a variety of other microbes such as bacteria or other fungi. Studying fungal interactions at the cellular level, however, can be challenging owing to the black box-like nature of soil. New microfluidic tools are being developed for the study of fungal interactions; two platforms designed to study bacterial-fungal and fungal-fungal interactions are highlighted. Within these microchannels, fungal-microbial interactions can be monitored in controlled physico-chemical environments at higher temporal and spatial resolution than previously possible. Application of these tools have yielded numerous novel biological insights, such as the observation of bacterial polar attachment to hyphae or revealing uncharacterised fungal-fungal antagonisms. A key feature of these methodologies regards the ease of use of this tool by non-experts, yielding highly translatable technologies for use in microbiology labs.
    MeSH term(s) Bacteria ; Ecosystem ; Fungi ; Microbial Interactions ; Microfluidics ; Soil/chemistry ; Soil Microbiology
    Chemical Substances Soil
    Language English
    Publishing date 2022-06-23
    Publishing country United States
    Document type Journal Article ; Video-Audio Media ; Research Support, Non-U.S. Gov't
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/63917
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A New Model: Psychogeriatric Assertive Community Treatment.

    Stanley, Claire / Stergiopoulos, Vicky / Colman, Sarah

    Psychiatric services (Washington, D.C.)

    2022  Volume 74, Issue 1, Page(s) 104–105

    MeSH term(s) Humans ; Community Mental Health Services ; Geriatric Psychiatry ; Mental Disorders/therapy ; Community-Institutional Relations
    Language English
    Publishing date 2022-10-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1220173-x
    ISSN 1557-9700 ; 1075-2730
    ISSN (online) 1557-9700
    ISSN 1075-2730
    DOI 10.1176/appi.ps.20220442
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: AMF-SporeChip

    Richter, Felix / Calonne-Salmon, Maryline / van der Heijden, Marcel G A / Declerck, Stéphane / Stanley, Claire E

    Lab on a chip

    2024  Volume 24, Issue 7, Page(s) 1930–1946

    Abstract: Arbuscular mycorrhizal fungi (AMF) form symbiotic associations with the majority of land plants and deliver a wide range of soil-based ecosystem services. Due to their conspicuous belowground lifestyle in a dark environment surrounded by soil particles, ... ...

    Abstract Arbuscular mycorrhizal fungi (AMF) form symbiotic associations with the majority of land plants and deliver a wide range of soil-based ecosystem services. Due to their conspicuous belowground lifestyle in a dark environment surrounded by soil particles, much is still to be learned about the influence of environmental (
    MeSH term(s) Mycorrhizae ; Ecosystem ; Symbiosis ; Hyphae ; Glomeromycota ; Soil ; Soil Microbiology
    Chemical Substances Soil
    Language English
    Publishing date 2024-03-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 2056646-3
    ISSN 1473-0189 ; 1473-0197
    ISSN (online) 1473-0189
    ISSN 1473-0197
    DOI 10.1039/d3lc00859b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Spores-on-a-chip: new frontiers for spore research.

    Bernier, Léa S / Junier, Pilar / Stan, Guy-Bart / Stanley, Claire E

    Trends in microbiology

    2022  Volume 30, Issue 6, Page(s) 515–518

    Abstract: In recent years, microfluidic technologies have become widespread in biological science. However, the suitability of this technique for understanding different aspects of spore research has hardly been considered. Herein, we review recent developments in ...

    Abstract In recent years, microfluidic technologies have become widespread in biological science. However, the suitability of this technique for understanding different aspects of spore research has hardly been considered. Herein, we review recent developments in 'spores-on-a-chip' technologies, highlighting how they could be exploited to drive new frontiers in spore research.
    MeSH term(s) Lab-On-A-Chip Devices ; Spores ; Spores, Bacterial
    Language English
    Publishing date 2022-03-25
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2022.03.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: South of the UES: Improving the ability of speech-language pathologists to detect oesophageal abnormalities during videofluoroscopy swallowing studies.

    Stanley, Claire / Rotman, Anthony / McKenzie, Dean / Malcolm, Louise / Paddle, Paul

    International journal of speech-language pathology

    2023  Volume 26, Issue 2, Page(s) 225–232

    Abstract: Purpose: With two-thirds of adults presenting for a videofluoroscopy swallow study (VFSS) with oesophageal abnormalities, it seems prudent to include visualisation of the oesophagus, in the context of the entire swallow process, to provide further ... ...

    Abstract Purpose: With two-thirds of adults presenting for a videofluoroscopy swallow study (VFSS) with oesophageal abnormalities, it seems prudent to include visualisation of the oesophagus, in the context of the entire swallow process, to provide further information to the diagnostic team. This study aims to evaluate the ability of speech-language pathologists (SLPs) to interpret oesophageal sweep on VFSS and the relative improvement in that ability with additional training.
    Method: One hundred SLPs attended training in oesophageal visualisation during VFSS, based on a previous study. Ten oesophageal sweep videos (five normal, five abnormal) with one 20 ml thin fluid barium bolus (19% w/v) were presented at baseline and following training. Raters were blinded to patient information other than age. Binary ratings were collected for oesophageal transit time (OTT), presence of stasis, redirection, and referral to other specialists.
    Result: Inter-rater reliability as measured by Fleiss' kappa improved for all parameters, reaching statistical significance for OTT (pre-test kappa = 0.34, post-test kappa = 0.73;
    Conclusion: Findings indicate that SLPs require training to accurately interpret an oesophageal sweep on VFSS. This supports the inclusion of education and training on both normal and abnormal oesophageal sweep patterns, and the use of standardised protocols for clinicians using oesophageal visualisation as part of the VFSS protocol.
    MeSH term(s) Adult ; Humans ; Deglutition Disorders/diagnosis ; Deglutition ; Reproducibility of Results ; Pathologists ; Speech ; Video Recording/methods
    Language English
    Publishing date 2023-07-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2402483-1
    ISSN 1754-9515 ; 1754-9507
    ISSN (online) 1754-9515
    ISSN 1754-9507
    DOI 10.1080/17549507.2023.2225801
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Microfluidic tools for probing fungal-microbial interactions at the cellular level

    Masters-Clark, Emily / Clark, Amelia J. / Stanley, Claire E.

    Journal of visualized experiments. 2022 June 23, , no. 184

    2022  

    Abstract: Filamentous fungi are successful inhabitants of soil and play a major role in soil ecosystems, such as in the decomposition of organic and inorganic matter, as well as regulation of nutrient levels. There they also find numerous opportunities to interact ...

    Abstract Filamentous fungi are successful inhabitants of soil and play a major role in soil ecosystems, such as in the decomposition of organic and inorganic matter, as well as regulation of nutrient levels. There they also find numerous opportunities to interact with a variety of other microbes such as bacteria or other fungi. Studying fungal interactions at the cellular level, however, can be challenging owing to the black box-like nature of soil. New microfluidic tools are being developed for the study of fungal interactions; two platforms designed to study bacterial-fungal and fungal-fungal interactions are highlighted. Within these microchannels, fungal-microbial interactions can be monitored in controlled physico-chemical environments at higher temporal and spatial resolution than previously possible. Application of these tools have yielded numerous novel biological insights, such as the observation of bacterial polar attachment to hyphae or revealing uncharacterised fungal-fungal antagonisms. A key feature of these methodologies regards the ease of use of this tool by non-experts, yielding highly translatable technologies for use in microbiology labs.
    Keywords fungi ; hyphae ; inorganic matter ; soil
    Language English
    Dates of publication 2022-0623
    Size p. e63917.
    Publishing place Journal of Visualized Experiments
    Document type Article
    ZDB-ID 2259946-0
    ISSN 1940-087X
    ISSN 1940-087X
    DOI 10.3791/63917
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Spores-on-a-chip: new frontiers for spore research

    Bernier, Léa S. / Junier, Pilar / Stan, Guy-Bart / Stanley, Claire E.

    Trends in microbiology. 2022 June, v. 30, no. 6

    2022  

    Abstract: In recent years, microfluidic technologies have become widespread in biological science. However, the suitability of this technique for understanding different aspects of spore research has hardly been considered. Herein, we review recent developments in ...

    Abstract In recent years, microfluidic technologies have become widespread in biological science. However, the suitability of this technique for understanding different aspects of spore research has hardly been considered. Herein, we review recent developments in 'spores-on-a-chip' technologies, highlighting how they could be exploited to drive new frontiers in spore research.
    Keywords methodology ; microbiology ; microfluidic technology ; spores
    Language English
    Dates of publication 2022-06
    Size p. 515-518.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2022.03.003
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Detecting Aspiration During FEES with Narrow Band Imaging in a Clinical Setting.

    Stanley, Claire / Paddle, Paul / Griffiths, Susie / Safdar, Adnan / Phyland, Debra

    Dysphagia

    2021  Volume 37, Issue 3, Page(s) 591–600

    Abstract: The use of narrow band imaging (NBI) during flexible endoscopic evaluation of swallowing (FEES) is recognised as an emerging technology to improve the contrast of the test fluid during endoscopic dysphagia evaluation. This study tested the hypothesis ... ...

    Abstract The use of narrow band imaging (NBI) during flexible endoscopic evaluation of swallowing (FEES) is recognised as an emerging technology to improve the contrast of the test fluid during endoscopic dysphagia evaluation. This study tested the hypothesis that the use of NBI in FEES would improve the detection of laryngeal penetration and aspiration in patients with unilateral vocal fold paralysis/paresis (UVFP), a typically difficult population in which to detect the presence of aspiration with FEES. Twenty-one consecutive outpatients with UVFP were evaluated with FEES using white light (WL) and NBI under 150 test conditions (75 WL & 75 NBI). Three speech pathologists, highly experienced in FEES using WL but novices to using NBI, rated laryngeal penetration and aspiration for green dyed thin fluid (5 ml and 90 ml) and mildly thick fluid (5 ml) milk, and were compared to two raters more experienced in using NBI during FEES. Laryngeal penetration and aspiration were significantly higher for larger volumes (90 ml) (p < 0.05). With NBI-naïve raters, there was a trend towards lower intra-rater and inter-rater reliability compared to WL on all bolus trials reaching significance on mildly thick fluid (p < 0.01). There was lower rater confidence when using NBI compared to WL in NBI-naïve raters to detect aspiration (p < 0.01). Sensitivity was lower regardless of NBI experience; 80.77-84.21% with WL compared to 46.15-50.00% with NBI. Findings indicate that the improved contrast of a dyed opaque milk trial under WL may negate the potential benefits of using NBI to increase the contrast of the test fluid and supports the use of an opaque test fluid such as milk. NBI may also not be as useful to clinicians with no experience with the altered light condition, and can result in lower sensitivity in even the experienced user.
    MeSH term(s) Coloring Agents ; Deglutition ; Deglutition Disorders/diagnostic imaging ; Endoscopes ; Humans ; Narrow Band Imaging/methods ; Reproducibility of Results
    Chemical Substances Coloring Agents
    Language English
    Publishing date 2021-05-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 632764-3
    ISSN 1432-0460 ; 0179-051X
    ISSN (online) 1432-0460
    ISSN 0179-051X
    DOI 10.1007/s00455-021-10309-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: pH Distribution along Growing Fungal Hyphae at Microscale.

    Xiong, Bi-Jing / Stanley, Claire E / Dusny, Christian / Schlosser, Dietmar / Harms, Hauke / Wick, Lukas Y

    Journal of fungi (Basel, Switzerland)

    2022  Volume 8, Issue 6

    Abstract: Creating unique microenvironments, hyphal surfaces and their surroundings allow for spatially distinct microbial interactions and functions at the microscale. Using a microfluidic system and pH-sensitive whole-cell bioreporters (Synechocystis sp. PCC6803) ...

    Abstract Creating unique microenvironments, hyphal surfaces and their surroundings allow for spatially distinct microbial interactions and functions at the microscale. Using a microfluidic system and pH-sensitive whole-cell bioreporters (Synechocystis sp. PCC6803) attached to hyphae, we spatially resolved the pH along surfaces of growing hyphae of the basidiomycete Coprinopsis cinerea. Time-lapse microscopy analysis of ratiometric fluorescence signals of >2400 individual bioreporters revealed an overall pH drop from 6.3 ± 0.4 (n = 2441) to 5.0 ± 0.3 (n = 2497) within 7 h after pH bioreporter loading to hyphal surfaces. The pH along hyphal surfaces varied significantly (p < 0.05), with pH at hyphal tips being on average ~0.8 pH units lower than at more mature hyphal parts near the entrance of the microfluidic observation chamber. Our data represent the first dynamic in vitro analysis of surface pH along growing hyphae at the micrometre scale. Such knowledge may improve our understanding of spatial, pH-dependent hyphal processes, such as the degradation of organic matter or mineral weathering.
    Language English
    Publishing date 2022-06-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof8060599
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi.

    Richter, Felix / Bindschedler, Saskia / Calonne-Salmon, Maryline / Declerck, Stéphane / Junier, Pilar / Stanley, Claire E

    FEMS microbiology reviews

    2022  

    Abstract: Fungi, with their enormous diversity, bear essential roles both in nature and our everyday lives. They inhabit a range of ecosystems, such as soil, where they are involved in organic matter degradation and bioremediation processes. More recently, fungi ... ...

    Abstract Fungi, with their enormous diversity, bear essential roles both in nature and our everyday lives. They inhabit a range of ecosystems, such as soil, where they are involved in organic matter degradation and bioremediation processes. More recently, fungi have been recognised as key components of the microbiome in other eukaryotes, such as humans, where they play a fundamental role not only in human pathogenesis, but also likely as commensals. In the food sector, fungi are used either directly or as fermenting agents and are often key players in the biotechnological industry, where they are responsible for the production of both bulk chemicals and antibiotics. Although the macroscopic fruiting bodies are immediately recognisable by most observers, the structure, function and interactions of fungi with other microbes at the microscopic scale still remain largely hidden. Herein, we shed light on new advances in the emerging field of Fungi-on-a-Chip microfluidic technologies for single-cell studies on fungi. We discuss the development and application of microfluidic tools in the fields of medicine and biotechnology, as well as in-depth biological studies having significance for ecology and general natural processes. Finally, a future perspective is provided, highlighting new frontiers in which microfluidic technology can benefit this field.
    Language English
    Publishing date 2022-08-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 283740-7
    ISSN 1574-6976 ; 0168-6445
    ISSN (online) 1574-6976
    ISSN 0168-6445
    DOI 10.1093/femsre/fuac039
    Database MEDical Literature Analysis and Retrieval System OnLINE

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