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Article ; Online: Sensing the wavefront of x-ray free-electron lasers using aerosol spheres.

Loh, N Duane / Starodub, Dmitri / Lomb, Lukas / Hampton, Christina Y / Martin, Andrew V / Sierra, Raymond G / Barty, Anton / Aquila, Andrew / Schulz, Joachim / Steinbrener, Jan / Shoeman, Robert L / Kassemeyer, Stephan / Bostedt, Christoph / Bozek, John / Epp, Sascha W / Erk, Benjamin / Hartmann, Robert / Rolles, Daniel / Rudenko, Artem /

Rudek, Benedikt / Foucar, Lutz / Kimmel, Nils / Weidenspointner, Georg / Hauser, Guenter / Holl, Peter / Pedersoli, Emanuele / Liang, Mengning / Hunter, Mark S / Gumprecht, Lars / Coppola, Nicola / Wunderer, Cornelia / Graafsma, Heinz / Maia, Filipe R N C / Ekeberg, Tomas / Hantke, Max / Fleckenstein, Holger / Hirsemann, Helmut / Nass, Karol / White, Thomas A / Tobias, Herbert J / Farquar, George R / Benner, W Henry / Hau-Riege, Stefan / Reich, Christian / Hartmann, Andreas / Soltau, Heike / Marchesini, Stefano / Bajt, Sasa / Barthelmess, Miriam / Strueder, Lothar / Ullrich, Joachim / Bucksbaum, Philip / Frank, Matthias / Schlichting, Ilme / Chapman, Henry N / Bogan, Michael J

Optics express

2013 Volume 21, Issue 10, Page(s) 12385–12394

Abstract: Characterizing intense, focused x-ray free electron laser (FEL) pulses is crucial for their use in diffractive imaging. We describe how the distribution of average phase tilts and intensities on hard x-ray pulses with peak intensities of 10(21) W/m(2) ... ...

Abstract	Characterizing intense, focused x-ray free electron laser (FEL) pulses is crucial for their use in diffractive imaging. We describe how the distribution of average phase tilts and intensities on hard x-ray pulses with peak intensities of 10(21) W/m(2) can be retrieved from an ensemble of diffraction patterns produced by 70 nm-radius polystyrene spheres, in a manner that mimics wavefront sensors. Besides showing that an adaptive geometric correction may be necessary for diffraction data from randomly injected sample sources, our paper demonstrates the possibility of collecting statistics on structured pulses using only the diffraction patterns they generate and highlights the imperative to study its impact on single-particle diffractive imaging.
MeSH term(s)	Aerosols/analysis ; Aerosols/chemistry ; Electrons ; Equipment Design ; Equipment Failure Analysis ; Lasers ; Microspheres ; Photometry/methods ; Refractometry/methods ; Surface Plasmon Resonance/methods ; X-Rays
Chemical Substances	Aerosols
Language	English
Publishing date	2013-05-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.21.012385
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Toward unsupervised single-shot diffractive imaging of heterogeneous particles using X-ray free-electron lasers.

Park, Hyung Joo / Loh, N Duane / Sierra, Raymond G / Hampton, Christina Y / Starodub, Dmitri / Martin, Andrew V / Barty, Anton / Aquila, Andrew / Schulz, Joachim / Steinbrener, Jan / Shoeman, Robert L / Lomb, Lukas / Kassemeyer, Stephan / Bostedt, Christoph / Bozek, John / Epp, Sascha W / Erk, Benjamin / Hartmann, Robert / Rolles, Daniel /

Rudenko, Artem / Rudek, Benedikt / Foucar, Lutz / Kimmel, Nils / Weidenspointner, Georg / Hauser, Guenter / Holl, Peter / Pedersoli, Emanuele / Liang, Mengning / Hunter, Mark S / Gumprecht, Lars / Coppola, Nicola / Wunderer, Cornelia / Graafsma, Heinz / Maia, Filipe R N C / Ekeberg, Tomas / Hantke, Max / Fleckenstein, Holger / Hirsemann, Helmut / Nass, Karol / Tobias, Herbert J / Farquar, George R / Benner, W Henry / Hau-Riege, Stefan / Reich, Christian / Hartmann, Andreas / Soltau, Heike / Marchesini, Stefano / Bajt, Sasa / Barthelmess, Miriam / Strueder, Lothar / Ullrich, Joachim / Bucksbaum, Philip / Frank, Matthias / Schlichting, Ilme / Chapman, Henry N / Bogan, Michael J / Elser, Veit

Optics express

2013 Volume 21, Issue 23, Page(s) 28729–28742

Abstract: Single shot diffraction imaging experiments via X-ray free-electron lasers can generate as many as hundreds of thousands of diffraction patterns of scattering objects. Recovering the real space contrast of a scattering object from these patterns ... ...

Abstract	Single shot diffraction imaging experiments via X-ray free-electron lasers can generate as many as hundreds of thousands of diffraction patterns of scattering objects. Recovering the real space contrast of a scattering object from these patterns currently requires a reconstruction process with user guidance in a number of steps, introducing severe bottlenecks in data processing. We present a series of measures that replace user guidance with algorithms that reconstruct contrasts in an unsupervised fashion. We demonstrate the feasibility of automating the reconstruction process by generating hundreds of contrasts obtained from soot particle diffraction experiments.
Language	English
Publishing date	2013-11-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.21.028729
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Article ; Online: Nanoflow electrospinning serial femtosecond crystallography.

Sierra, Raymond G / Laksmono, Hartawan / Kern, Jan / Tran, Rosalie / Hattne, Johan / Alonso-Mori, Roberto / Lassalle-Kaiser, Benedikt / Glöckner, Carina / Hellmich, Julia / Schafer, Donald W / Echols, Nathaniel / Gildea, Richard J / Grosse-Kunstleve, Ralf W / Sellberg, Jonas / McQueen, Trevor A / Fry, Alan R / Messerschmidt, Marc M / Miahnahri, Alan / Seibert, M Marvin /

Hampton, Christina Y / Starodub, Dmitri / Loh, N Duane / Sokaras, Dimosthenis / Weng, Tsu-Chien / Zwart, Petrus H / Glatzel, Pieter / Milathianaki, Despina / White, William E / Adams, Paul D / Williams, Garth J / Boutet, Sébastien / Zouni, Athina / Messinger, Johannes / Sauter, Nicholas K / Bergmann, Uwe / Yano, Junko / Yachandra, Vittal K / Bogan, Michael J

Acta crystallographica. Section D, Biological crystallography

2012 Volume 68, Issue Pt 11, Page(s) 1584–1587

Abstract: An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 ... ...

Abstract	An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond 4 Å resolution, producing 14,000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.
MeSH term(s)	Crystallization ; Crystallography, X-Ray/economics ; Crystallography, X-Ray/instrumentation ; Electromagnetic Fields ; Equipment Design ; Kinetics ; Lasers ; Sample Size ; Thermolysin/chemistry
Chemical Substances	Thermolysin (EC 3.4.24.27)
Language	English
Publishing date	2012-10-18
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2020492-9
ISSN	1399-0047 ; 0907-4449
ISSN (online)	1399-0047
ISSN	0907-4449
DOI	10.1107/S0907444912038152
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Article ; Online: Femtosecond free-electron laser x-ray diffraction data sets for algorithm development.

Kassemeyer, Stephan / Steinbrener, Jan / Lomb, Lukas / Hartmann, Elisabeth / Aquila, Andrew / Barty, Anton / Martin, Andrew V / Hampton, Christina Y / Bajt, Saša / Barthelmess, Miriam / Barends, Thomas R M / Bostedt, Christoph / Bott, Mario / Bozek, John D / Coppola, Nicola / Cryle, Max / DePonte, Daniel P / Doak, R Bruce / Epp, Sascha W /

Erk, Benjamin / Fleckenstein, Holger / Foucar, Lutz / Graafsma, Heinz / Gumprecht, Lars / Hartmann, Andreas / Hartmann, Robert / Hauser, Günter / Hirsemann, Helmut / Hömke, André / Holl, Peter / Jönsson, Olof / Kimmel, Nils / Krasniqi, Faton / Liang, Mengning / Maia, Filipe R N C / Marchesini, Stefano / Nass, Karol / Reich, Christian / Rolles, Daniel / Rudek, Benedikt / Rudenko, Artem / Schmidt, Carlo / Schulz, Joachim / Shoeman, Robert L / Sierra, Raymond G / Soltau, Heike / Spence, John C H / Starodub, Dmitri / Stellato, Francesco / Stern, Stephan / Stier, Gunter / Svenda, Martin / Weidenspointner, Georg / Weierstall, Uwe / White, Thomas A / Wunderer, Cornelia / Frank, Matthias / Chapman, Henry N / Ullrich, Joachim / Strüder, Lothar / Bogan, Michael J / Schlichting, Ilme

Optics express

2012 Volume 20, Issue 4, Page(s) 4149–4158

Abstract: We describe femtosecond X-ray diffraction data sets of viruses and nanoparticles collected at the Linac Coherent Light Source. The data establish the first large benchmark data sets for coherent diffraction methods freely available to the public, to ... ...

Abstract	We describe femtosecond X-ray diffraction data sets of viruses and nanoparticles collected at the Linac Coherent Light Source. The data establish the first large benchmark data sets for coherent diffraction methods freely available to the public, to bolster the development of algorithms that are essential for developing this novel approach as a useful imaging technique. Applications are 2D reconstructions, orientation classification and finally 3D imaging by assembling 2D patterns into a 3D diffraction volume.
Language	English
Publishing date	2012-02-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.20.004149
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Article ; Online: Lipidic phase membrane protein serial femtosecond crystallography.

Johansson, Linda C / Arnlund, David / White, Thomas A / Katona, Gergely / Deponte, Daniel P / Weierstall, Uwe / Doak, R Bruce / Shoeman, Robert L / Lomb, Lukas / Malmerberg, Erik / Davidsson, Jan / Nass, Karol / Liang, Mengning / Andreasson, Jakob / Aquila, Andrew / Bajt, Saša / Barthelmess, Miriam / Barty, Anton / Bogan, Michael J /

Bostedt, Christoph / Bozek, John D / Caleman, Carl / Coffee, Ryan / Coppola, Nicola / Ekeberg, Tomas / Epp, Sascha W / Erk, Benjamin / Fleckenstein, Holger / Foucar, Lutz / Graafsma, Heinz / Gumprecht, Lars / Hajdu, Janos / Hampton, Christina Y / Hartmann, Robert / Hartmann, Andreas / Hauser, Günter / Hirsemann, Helmut / Holl, Peter / Hunter, Mark S / Kassemeyer, Stephan / Kimmel, Nils / Kirian, Richard A / Maia, Filipe R N C / Marchesini, Stefano / Martin, Andrew V / Reich, Christian / Rolles, Daniel / Rudek, Benedikt / Rudenko, Artem / Schlichting, Ilme / Schulz, Joachim / Seibert, M Marvin / Sierra, Raymond G / Soltau, Heike / Starodub, Dmitri / Stellato, Francesco / Stern, Stephan / Strüder, Lothar / Timneanu, Nicusor / Ullrich, Joachim / Wahlgren, Weixiao Y / Wang, Xiaoyu / Weidenspointner, Georg / Wunderer, Cornelia / Fromme, Petra / Chapman, Henry N / Spence, John C H / Neutze, Richard

Nature methods

2012 Volume 9, Issue 3, Page(s) 263–265

Abstract: X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from ... ...

Abstract	X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.
MeSH term(s)	Crystallography, X-Ray/methods ; Lipid Bilayers/chemistry ; Membrane Proteins/chemistry ; Membrane Proteins/ultrastructure ; Protein Binding ; Protein Conformation/radiation effects ; X-Rays
Chemical Substances	Lipid Bilayers ; Membrane Proteins
Language	English
Publishing date	2012-01-29
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2169522-2
ISSN	1548-7105 ; 1548-7091
ISSN (online)	1548-7105
ISSN	1548-7091
DOI	10.1038/nmeth.1867
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Article ; Online: Unsupervised classification of single-particle X-ray diffraction snapshots by spectral clustering.

Yoon, Chun Hong / Schwander, Peter / Abergel, Chantal / Andersson, Inger / Andreasson, Jakob / Aquila, Andrew / Bajt, Saša / Barthelmess, Miriam / Barty, Anton / Bogan, Michael J / Bostedt, Christoph / Bozek, John / Chapman, Henry N / Claverie, Jean-Michel / Coppola, Nicola / DePonte, Daniel P / Ekeberg, Tomas / Epp, Sascha W / Erk, Benjamin /

Fleckenstein, Holger / Foucar, Lutz / Graafsma, Heinz / Gumprecht, Lars / Hajdu, Janos / Hampton, Christina Y / Hartmann, Andreas / Hartmann, Elisabeth / Hartmann, Robert / Hauser, Gunter / Hirsemann, Helmut / Holl, Peter / Kassemeyer, Stephan / Kimmel, Nils / Kiskinova, Maya / Liang, Mengning / Loh, Ne-Te Duane / Lomb, Lukas / Maia, Filipe R N C / Martin, Andrew V / Nass, Karol / Pedersoli, Emanuele / Reich, Christian / Rolles, Daniel / Rudek, Benedikt / Rudenko, Artem / Schlichting, Ilme / Schulz, Joachim / Seibert, Marvin / Seltzer, Virginie / Shoeman, Robert L / Sierra, Raymond G / Soltau, Heike / Starodub, Dmitri / Steinbrener, Jan / Stier, Gunter / Strüder, Lothar / Svenda, Martin / Ullrich, Joachim / Weidenspointner, Georg / White, Thomas A / Wunderer, Cornelia / Ourmazd, Abbas

Optics express

2011 Volume 19, Issue 17, Page(s) 16542–16549

Abstract: Single-particle experiments using X-ray Free Electron Lasers produce more than 10(5) snapshots per hour, consisting of an admixture of blank shots (no particle intercepted), and exposures of one or more particles. Experimental data sets also often ... ...

Abstract	Single-particle experiments using X-ray Free Electron Lasers produce more than 10(5) snapshots per hour, consisting of an admixture of blank shots (no particle intercepted), and exposures of one or more particles. Experimental data sets also often contain unintentional contamination with different species. We present an unsupervised method able to sort experimental snapshots without recourse to templates, specific noise models, or user-directed learning. The results show 90% agreement with manual classification.
Language	English
Publishing date	2011-08-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.19.016542
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Article ; Online: Time-resolved protein nanocrystallography using an X-ray free-electron laser.

Aquila, Andrew / Hunter, Mark S / Doak, R Bruce / Kirian, Richard A / Fromme, Petra / White, Thomas A / Andreasson, Jakob / Arnlund, David / Bajt, Saša / Barends, Thomas R M / Barthelmess, Miriam / Bogan, Michael J / Bostedt, Christoph / Bottin, Hervé / Bozek, John D / Caleman, Carl / Coppola, Nicola / Davidsson, Jan / DePonte, Daniel P /

Elser, Veit / Epp, Sascha W / Erk, Benjamin / Fleckenstein, Holger / Foucar, Lutz / Frank, Matthias / Fromme, Raimund / Graafsma, Heinz / Grotjohann, Ingo / Gumprecht, Lars / Hajdu, Janos / Hampton, Christina Y / Hartmann, Andreas / Hartmann, Robert / Hau-Riege, Stefan / Hauser, Günter / Hirsemann, Helmut / Holl, Peter / Holton, James M / Hömke, André / Johansson, Linda / Kimmel, Nils / Kassemeyer, Stephan / Krasniqi, Faton / Kühnel, Kai-Uwe / Liang, Mengning / Lomb, Lukas / Malmerberg, Erik / Marchesini, Stefano / Martin, Andrew V / Maia, Filipe R N C / Messerschmidt, Marc / Nass, Karol / Reich, Christian / Neutze, Richard / Rolles, Daniel / Rudek, Benedikt / Rudenko, Artem / Schlichting, Ilme / Schmidt, Carlo / Schmidt, Kevin E / Schulz, Joachim / Seibert, M Marvin / Shoeman, Robert L / Sierra, Raymond / Soltau, Heike / Starodub, Dmitri / Stellato, Francesco / Stern, Stephan / Strüder, Lothar / Timneanu, Nicusor / Ullrich, Joachim / Wang, Xiaoyu / Williams, Garth J / Weidenspointner, Georg / Weierstall, Uwe / Wunderer, Cornelia / Barty, Anton / Spence, John C H / Chapman, Henry N

Optics express

2012 Volume 20, Issue 3, Page(s) 2706–2716

Abstract: We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. ... ...

Abstract	We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light-induced changes of Photosystem I-Ferredoxin co-crystals were observed at time delays of 5 to 10 µs after excitation. The result correlates with the microsecond kinetics of electron transfer from Photosystem I to ferredoxin. The undocking process that follows the electron transfer leads to large rearrangements in the crystals that will terminally lead to the disintegration of the crystals. We describe the experimental setup and obtain the first time-resolved femtosecond serial X-ray crystallography results from an irreversible photo-chemical reaction at the Linac Coherent Light Source. This technique opens the door to time-resolved structural studies of reaction dynamics in biological systems.
MeSH term(s)	Crystallography, X-Ray/methods ; Electrons ; Ferredoxins/ultrastructure ; Lasers ; Nanostructures/ultrastructure ; Protein Conformation ; X-Ray Diffraction/methods ; X-Rays
Chemical Substances	Ferredoxins
Language	English
Publishing date	2012-02-13
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.20.002706
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Article ; Online: Femtosecond X-ray protein nanocrystallography.

Chapman, Henry N / Fromme, Petra / Barty, Anton / White, Thomas A / Kirian, Richard A / Aquila, Andrew / Hunter, Mark S / Schulz, Joachim / DePonte, Daniel P / Weierstall, Uwe / Doak, R Bruce / Maia, Filipe R N C / Martin, Andrew V / Schlichting, Ilme / Lomb, Lukas / Coppola, Nicola / Shoeman, Robert L / Epp, Sascha W / Hartmann, Robert /

Rolles, Daniel / Rudenko, Artem / Foucar, Lutz / Kimmel, Nils / Weidenspointner, Georg / Holl, Peter / Liang, Mengning / Barthelmess, Miriam / Caleman, Carl / Boutet, Sébastien / Bogan, Michael J / Krzywinski, Jacek / Bostedt, Christoph / Bajt, Saša / Gumprecht, Lars / Rudek, Benedikt / Erk, Benjamin / Schmidt, Carlo / Hömke, André / Reich, Christian / Pietschner, Daniel / Strüder, Lothar / Hauser, Günter / Gorke, Hubert / Ullrich, Joachim / Herrmann, Sven / Schaller, Gerhard / Schopper, Florian / Soltau, Heike / Kühnel, Kai-Uwe / Messerschmidt, Marc / Bozek, John D / Hau-Riege, Stefan P / Frank, Matthias / Hampton, Christina Y / Sierra, Raymond G / Starodub, Dmitri / Williams, Garth J / Hajdu, Janos / Timneanu, Nicusor / Seibert, M Marvin / Andreasson, Jakob / Rocker, Andrea / Jönsson, Olof / Svenda, Martin / Stern, Stephan / Nass, Karol / Andritschke, Robert / Schröter, Claus-Dieter / Krasniqi, Faton / Bott, Mario / Schmidt, Kevin E / Wang, Xiaoyu / Grotjohann, Ingo / Holton, James M / Barends, Thomas R M / Neutze, Richard / Marchesini, Stefano / Fromme, Raimund / Schorb, Sebastian / Rupp, Daniela / Adolph, Marcus / Gorkhover, Tais / Andersson, Inger / Hirsemann, Helmut / Potdevin, Guillaume / Graafsma, Heinz / Nilsson, Björn / Spence, John C H

Nature

2011 Volume 470, Issue 7332, Page(s) 73–77

Abstract: X-ray crystallography provides the vast majority of macromolecular structures, but the success of the method relies on growing crystals of sufficient size. In conventional measurements, the necessary increase in X-ray dose to record data from crystals ... ...

Abstract	X-ray crystallography provides the vast majority of macromolecular structures, but the success of the method relies on growing crystals of sufficient size. In conventional measurements, the necessary increase in X-ray dose to record data from crystals that are too small leads to extensive damage before a diffraction signal can be recorded. It is particularly challenging to obtain large, well-diffracting crystals of membrane proteins, for which fewer than 300 unique structures have been determined despite their importance in all living cells. Here we present a method for structure determination where single-crystal X-ray diffraction 'snapshots' are collected from a fully hydrated stream of nanocrystals using femtosecond pulses from a hard-X-ray free-electron laser, the Linac Coherent Light Source. We prove this concept with nanocrystals of photosystem I, one of the largest membrane protein complexes. More than 3,000,000 diffraction patterns were collected in this study, and a three-dimensional data set was assembled from individual photosystem I nanocrystals (∼200 nm to 2 μm in size). We mitigate the problem of radiation damage in crystallography by using pulses briefer than the timescale of most damage processes. This offers a new approach to structure determination of macromolecules that do not yield crystals of sufficient size for studies using conventional radiation sources or are particularly sensitive to radiation damage.
MeSH term(s)	Crystallography, X-Ray/instrumentation ; Crystallography, X-Ray/methods ; Lasers ; Models, Molecular ; Nanoparticles/chemistry ; Nanotechnology/instrumentation ; Nanotechnology/methods ; Photosystem I Protein Complex/chemistry ; Protein Conformation ; Time Factors ; X-Rays
Chemical Substances	Photosystem I Protein Complex
Language	English
Publishing date	2011-02-15
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/nature09750
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Article ; Online: Single mimivirus particles intercepted and imaged with an X-ray laser.

Seibert, M Marvin / Ekeberg, Tomas / Maia, Filipe R N C / Svenda, Martin / Andreasson, Jakob / Jönsson, Olof / Odić, Duško / Iwan, Bianca / Rocker, Andrea / Westphal, Daniel / Hantke, Max / DePonte, Daniel P / Barty, Anton / Schulz, Joachim / Gumprecht, Lars / Coppola, Nicola / Aquila, Andrew / Liang, Mengning / White, Thomas A /

Martin, Andrew / Caleman, Carl / Stern, Stephan / Abergel, Chantal / Seltzer, Virginie / Claverie, Jean-Michel / Bostedt, Christoph / Bozek, John D / Boutet, Sébastien / Miahnahri, A Alan / Messerschmidt, Marc / Krzywinski, Jacek / Williams, Garth / Hodgson, Keith O / Bogan, Michael J / Hampton, Christina Y / Sierra, Raymond G / Starodub, Dmitri / Andersson, Inger / Bajt, Saša / Barthelmess, Miriam / Spence, John C H / Fromme, Petra / Weierstall, Uwe / Kirian, Richard / Hunter, Mark / Doak, R Bruce / Marchesini, Stefano / Hau-Riege, Stefan P / Frank, Matthias / Shoeman, Robert L / Lomb, Lukas / Epp, Sascha W / Hartmann, Robert / Rolles, Daniel / Rudenko, Artem / Schmidt, Carlo / Foucar, Lutz / Kimmel, Nils / Holl, Peter / Rudek, Benedikt / Erk, Benjamin / Hömke, André / Reich, Christian / Pietschner, Daniel / Weidenspointner, Georg / Strüder, Lothar / Hauser, Günter / Gorke, Hubert / Ullrich, Joachim / Schlichting, Ilme / Herrmann, Sven / Schaller, Gerhard / Schopper, Florian / Soltau, Heike / Kühnel, Kai-Uwe / Andritschke, Robert / Schröter, Claus-Dieter / Krasniqi, Faton / Bott, Mario / Schorb, Sebastian / Rupp, Daniela / Adolph, Marcus / Gorkhover, Tais / Hirsemann, Helmut / Potdevin, Guillaume / Graafsma, Heinz / Nilsson, Björn / Chapman, Henry N / Hajdu, Janos

Nature

2011 Volume 470, Issue 7332, Page(s) 78–81

Abstract: X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain ... ...

Abstract	X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000 K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.
MeSH term(s)	Electrons ; Hot Temperature ; Lasers ; Mimiviridae/chemistry ; Photons ; Time Factors ; X-Ray Diffraction/instrumentation ; X-Ray Diffraction/methods ; X-Rays
Language	English
Publishing date	2011-02-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/nature09748
Database	MEDical Literature Analysis and Retrieval System OnLINE

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