LIVIVO - Search results -

Search results

Result 1 - 10 of total 25

Search options

Article ; Online: Osteonecrosis risk after steroids-related treatment of COVID-19 is not negligible: A cross sectional study.

Koutalos, Antonios A / Koskiniotis, Alexandros / Rountas, Christos / Konstantinou, Efstathios / Georgiadou, Sarah / Stefos, Aggelos / Gatselis, Nikolaos K / Dalekos, George N / Malizos, Konstantinos N

European journal of internal medicine

2024 Volume 123, Page(s) 127–131

Abstract: Background & aims: During the pandemic, steroids use at various dosages and durations for the treatment of COVID-19 patients, especially in hospitalized patients, was a common and effective strategy. However, steroid administration is associated with ... ...

Abstract	Background & aims: During the pandemic, steroids use at various dosages and durations for the treatment of COVID-19 patients, especially in hospitalized patients, was a common and effective strategy. However, steroid administration is associated with osteonecrosis as an adverse event. The aim of the study was to examine the prevalence of skeleton osteonecrosis in COVID-19 patients treated with or without steroids. Methods: Eighty randomly selected hospitalized COVID-19 patients were analyzed, of which 40 were managed with a published protocol including steroids and 40 did not receive steroids. Demographics and laboratory measurements including white blood cells count, C-reactive protein and ferritin were retrieved from the medical records. All patients underwent magnetic resonance imaging of the hips, shoulders, and knees. Subsequently, all patients were clinically examined and Oxford hip score (OHS) and EuroQol- 5 Dimension (EQ-5D-5 L) were documented. Results: Three patients (3/40; 7.5 %) treated with steroids were diagnosed with femoral head osteonecrosis. None of the patients in the non-steroid-treated group developed osteonecrosis. There were no differences between the two groups regarding OHS and EQ-5D-5 L. Patients with osteonecrosis had higher ferritin levels, received higher doses of corticosteroids (median dose 2200 mg), and had longer hospitalization. Conclusions: COVID-19-related therapy with steroids resulted in lower prevalence of osteonecrosis than that previously recorded in patients with severe acute respiratory syndrome caused by coronavirus-type-1. However, this risk seems not negligible and therefore, high clinical suspicion for early diagnosis is warranted, given the fact that a great proportion of hospitalized patients received steroids during the COVID-19 pandemic.
MeSH term(s)	Humans ; Male ; Female ; Middle Aged ; COVID-19/complications ; COVID-19/epidemiology ; Cross-Sectional Studies ; COVID-19 Drug Treatment ; Aged ; SARS-CoV-2 ; Osteonecrosis/chemically induced ; Osteonecrosis/epidemiology ; Osteonecrosis/diagnostic imaging ; Glucocorticoids/adverse effects ; Glucocorticoids/therapeutic use ; Glucocorticoids/administration & dosage ; Magnetic Resonance Imaging ; Femur Head Necrosis/chemically induced ; Femur Head Necrosis/epidemiology ; Prevalence
Chemical Substances	Glucocorticoids
Language	English
Publishing date	2024-01-28
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1038679-8
ISSN	1879-0828 ; 0953-6205
ISSN (online)	1879-0828
ISSN	0953-6205
DOI	10.1016/j.ejim.2024.01.006
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2608: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article ; Online: Rifampicin: not always an innocent drug.

Sveroni, Dafni / Stefos, Aggelos / Rigopoulou, Eirini I / Dalekos, Georgios N

BMJ case reports

2018 Volume 11, Issue 1

Abstract: Rifampicin has been widely used due to its broad antibacterial spectrum. Acute haemolysis is a rarely encountered complication of rifampicin. A 58-year-old woman was admitted to our department because of high-grade fever with rigors, accompanied by ... ...

Abstract	Rifampicin has been widely used due to its broad antibacterial spectrum. Acute haemolysis is a rarely encountered complication of rifampicin. A 58-year-old woman was admitted to our department because of high-grade fever with rigors, accompanied by abdominal and lumbar pain and laboratory evidence of acute haemolysis. She had been treated for brucellosis initially with doxycycline and streptomycin. Due to subsequent appearance of myositis, ciprofloxacin and rifampicin were added for treatment of localised brucellosis. After intravenous administration of rifampicin, the patient deteriorated significantly. After exclusion of other causes of haemolysis, autoimmune haemolytic anaemia related to rifampicin was established by strongly positive direct Coombs test. Drug withdrawal in conjunction with intravenous immune globulin and prednisolone resulted in resolution of haemolysis and no relapse in the ensuing 1-year period. Our case highlights the importance of recognising commonly administrative drugs as cause of haemolytic anaemia, that can often be life threatening.
MeSH term(s)	Abdominal Pain/etiology ; Anemia, Hemolytic, Autoimmune/chemically induced ; Anemia, Hemolytic, Autoimmune/drug therapy ; Anti-Bacterial Agents/administration & dosage ; Anti-Bacterial Agents/adverse effects ; Brucellosis/drug therapy ; Chills ; Female ; Fever ; Humans ; Immunoglobulins, Intravenous/therapeutic use ; Middle Aged ; Prednisolone/therapeutic use ; Rifampin/administration & dosage ; Rifampin/adverse effects ; Treatment Outcome
Chemical Substances	Anti-Bacterial Agents ; Immunoglobulins, Intravenous ; Prednisolone (9PHQ9Y1OLM) ; Rifampin (VJT6J7R4TR)
Language	English
Publishing date	2018-12-09
Publishing country	England
Document type	Case Reports ; Journal Article
ISSN	1757-790X
ISSN (online)	1757-790X
DOI	10.1136/bcr-2018-227356
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Plethora of Resistance Genes in Carbapenem-Resistant Gram-Negative Bacteria in Greece: No End to a Continuous Genetic Evolution

Tsilipounidaki, Katerina / Athanasakopoulou, Zoi / Müller, Elke / Burgold-Voigt, Sindy / Florou, Zoi / Braun, Sascha D. / Monecke, Stefan / Gatselis, Nikolaos K. / Zachou, Kalliopi / Stefos, Aggelos / Tsagalas, Ilias / Sofia, Marina / Spyrou, Vassiliki / Billinis, Charalambos / Dalekos, George N. / Ehricht, Ralf / Petinaki, Efthymia

Microorganisms. 2022 Jan. 13, v. 10, no. 1

2022

Abstract: Carbapenem-resistant Gram-negative bacteria are a public health threat that requires urgent action. The fact that these pathogens commonly also harbor resistance mechanisms for several other antimicrobial classes further reduces patient treatment options. ...

Abstract	Carbapenem-resistant Gram-negative bacteria are a public health threat that requires urgent action. The fact that these pathogens commonly also harbor resistance mechanisms for several other antimicrobial classes further reduces patient treatment options. The present study aimed to provide information regarding the multidrug resistance genetic background of carbapenem-resistant Gram-negative bacteria in Central Greece. Strains from a tertiary care hospital, collected during routine practice, were characterized using a DNA microarray-based assay. Various different resistance determinants for carbapenems, other beta-lactams, aminoglycosides, quinolones, trimethoprim, sulfonamides and macrolides were detected among isolates of the same sequence type. Eighteen different multidrug resistance genomic profiles were identified among the twenty-four K. pneumoniae ST258, seven different profiles among the eight K. pneumoniae ST11, four profiles among the six A. baumannii ST409 and two among the three K. oxytoca. This report describes the multidrug resistance genomic background of carbapenem-resistant Gram-negative bacteria from a tertiary care hospital in Central Greece, providing evidence of their continuous genetic evolution.
Keywords	DNA ; aminoglycosides ; carbapenems ; evolution ; genetic background ; genomics ; hospitals ; macrolides ; microarray technology ; multiple drug resistance ; patients ; public health ; quinolones ; trimethoprim ; Greece
Language	English
Dates of publication	2022-0113
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10010159
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Calprotectin serum levels on admission and during follow-up predict severity and outcome of patients with COVID-19: A prospective study.

Gatselis, Nikolaos K / Lyberopoulou, Aggeliki / Lygoura, Vasiliki / Giannoulis, George / Samakidou, Anna / Vaiou, Antonia / Antoniou, Katerina / Triantafyllou, Katerina / Stefos, Aggelos / Georgiadou, Sarah / Sagris, Dimitrios / Sveroni, Dafni / Gabeta, Stella / Ntaios, George / Norman, Gary L / Dalekos, George N

European journal of internal medicine

2023 Volume 122, Page(s) 78–85

Abstract: Background & aims: Calprotectin reflects neutrophil activation and is increased in various inflammatory conditions including severe COVID-19. However, serial serum calprotectin measurements in COVID-19 patients are limited. We assessed prospectively, ... ...

Abstract	Background & aims: Calprotectin reflects neutrophil activation and is increased in various inflammatory conditions including severe COVID-19. However, serial serum calprotectin measurements in COVID-19 patients are limited. We assessed prospectively, calprotectin levels as biomarker of severity/outcome of the disease and a COVID-19 monitoring parameter in a large cohort of consecutive COVID-19 patients. Methods: Calprotectin serum levels were measured in 736 patients (58.2 % males; median age 63-years; moderate disease, n = 292; severe, n = 444, intubated and/or died, n = 50). Patients were treated with combined immunotherapies according to our published local algorithm. The endpoint was the composite event of intubation due to severe respiratory failure (SRF)/COVID-19-related mortality. Results: Median (interquartile range) calprotectin levels were significantly higher in patients with severe disease [7(8.2) vs. 6.1(8.1)μg/mL, p = 0.015]. Calprotectin on admission was the only independent risk factor for intubation/death (HR=1.473, 95 %CI=1.003-2.165, p = 0.048) even after adjustment for age, sex, body mass index, comorbidities, neutrophils, lymphocytes, neutrophil to lymphocytes ratio, ferritin, and CRP. The area under the curve (AUC, 95 %CI) of calprotectin for prediction of intubation/death was 0.619 (0.531-0.708), with an optimal cut-off at 13 μg/mL (sensitivity: 44 %, specificity: 79 %, positive and negative predictive values: 13 % and 95 %, respectively). For intubated/died patients, paired comparisons from baseline to middle of hospitalization and subsequently to intubation/death showed significant increase of calprotectin (p = 0.009 and p < 0.001, respectively). Calprotectin alteration had the higher predictive ability for intubation/death [AUC (95 %CI):0.803 (0.664-0.943), p < 0.001]. Conclusions: Calprotectin levels on admission and their subsequent dynamic alterations could serve as indicator of COVID-19 severity and predict the occurrence of SRF and mortality.
MeSH term(s)	Male ; Humans ; Middle Aged ; Female ; Prospective Studies ; Follow-Up Studies ; Leukocyte L1 Antigen Complex ; COVID-19/therapy ; Biomarkers ; Retrospective Studies
Chemical Substances	Leukocyte L1 Antigen Complex ; Biomarkers
Language	English
Publishing date	2023-11-11
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1038679-8
ISSN	1879-0828 ; 0953-6205
ISSN (online)	1879-0828
ISSN	0953-6205
DOI	10.1016/j.ejim.2023.11.001
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2608: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Diabetes on sepsis outcomes in non-ICU patients: A cohort study and review of the literature.

Akinosoglou, Karolina / Kapsokosta, Georgia / Mouktaroudi, Maria / Rovina, Nikoletta / Kaldis, Vassileios / Stefos, Aggelos / Kontogiorgi, Marina / Giamarellos-Bourboulis, Evangelos / Gogos, Charalambos

Journal of diabetes and its complications

2020 Volume 35, Issue 1, Page(s) 107765

Abstract: Aims: We sought to determine whether primary outcomes differ between non-ICU septic patients with and without type 2 diabetes (T2D).: Methods: This study utilized the Hellenic Sepsis Study Group Registry, collecting nationwide data for sepsis ... ...

Abstract	Aims: We sought to determine whether primary outcomes differ between non-ICU septic patients with and without type 2 diabetes (T2D). Methods: This study utilized the Hellenic Sepsis Study Group Registry, collecting nationwide data for sepsis patients since 2006, and classified patients upon presence or absence of T2D. Patients were perfectly matched for a) Sepsis 3 definition criteria (including septic shock) b) gender, c) age, d) APACHE II score and e) Charlson's comorbidity index (CCI). Independent sample t-test and chi-square t-test was used to compare prognostic indices and primary outcomes. Results: Of 4320 initially included non-ICU sepsis patients, 812 were finally analysed, following match on criteria. Baseline characteristics were age 76 [±10.3] years, 46% male, APACHE II 15.5 [±6], CCI 5.1 [±1.8], 24% infection, 63.8% sepsis and 12.2% septic shock. No significant difference was noted between two groups in qSOFA, SOFA, or suPAR1 levels (p = 0.7, 0.1 & 0.3) respectively. Primary sepsis syndrome resolved in 70.9% of cases (p = 0.9), while mortality was 24% in 28-days time. Cause of death was similar between patients with and without T2D (sepsis 17.8% vs 15.8%, heart event 3.7% vs 3.2%, CNS event 0.5% vs 0.5%, malignancy 0.7% vs 2% respectively, p = 0.6). Conclusions: DM does not appear to negatively affect outcomes in septic patients not requiring ICU.
MeSH term(s)	Aged ; Aged, 80 and over ; Cohort Studies ; Diabetes Mellitus, Type 2 ; Female ; Humans ; Intensive Care Units ; Male ; Prognosis ; Sepsis/complications ; Sepsis/epidemiology ; Shock, Septic/complications ; Shock, Septic/epidemiology
Language	English
Publishing date	2020-10-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1105840-7
ISSN	1873-460X ; 1056-8727
ISSN (online)	1873-460X
ISSN	1056-8727
DOI	10.1016/j.jdiacomp.2020.107765
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2225: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Efficient management of secondary haemophagocytic lymphohistiocytosis with intravenous steroids and γ-immunoglobulin infusions.

Georgiadou, Sarah / Gatselis, Nikolaos K / Stefos, Aggelos / Zachou, Kalliopi / Makaritsis, Konstantinos / Rigopoulou, Eirini I / Dalekos, George N

World journal of clinical cases

2019 Volume 7, Issue 21, Page(s) 3394–3406

Abstract: Background: Secondary haemophagocytic lymphohistiocytosis (sHLH) is a rare life-threatening condition mainly associated with underlying infections, malignancies, and autoimmune or immune-mediated diseases.: Aim: To analyse all sHLH cases that were ... ...

Abstract	Background: Secondary haemophagocytic lymphohistiocytosis (sHLH) is a rare life-threatening condition mainly associated with underlying infections, malignancies, and autoimmune or immune-mediated diseases. Aim: To analyse all sHLH cases that were diagnosed and managed under real-world circumstances in our department focusing on the treatment schedule and the outcome. Methods: Prospectively collected data from all adult patients fulfilling the criteria of sHLH who diagnosed and managed from January 1, 2010 to June 1, 2018, in our department of the tertiary care university hospital of Larissa, Greece, were analysed retrospectively ( Results: Seventy-five patients (94%) reported fever > 38.5 °C, 47 (59%) had liver or spleen enlargement and 76 (95%) had ferritin > 500 ng/mL including 20 (25%) having considerably high levels (> 10000 ng/mL). Anaemia and thrombocytopenia occurred in 72% and leucopoenia in 47% of them. Underlying infections were diagnosed in 59 patients (74%) as follows: leishmaniasis alone in 15/80 (18.9%), leishmaniasis concurrently with Conclusion: sHLH still carries a remarkable morbidity and mortality. Underlying infections were the major cause and therefore, they should be thoroughly investigated in patients with sHLH. Early recognition and combination treatment with IVIG and corticosteroids seem an efficient treatment option with successful outcome in this life-threatening condition.
Language	English
Publishing date	2019-10-18
Publishing country	United States
Document type	Journal Article
ISSN	2307-8960
ISSN	2307-8960
DOI	10.12998/wjcc.v7.i21.3394
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Plethora of Resistance Genes in Carbapenem-Resistant Gram-Negative Bacteria in Greece: No End to a Continuous Genetic Evolution.

Tsilipounidaki, Katerina / Athanasakopoulou, Zoi / Müller, Elke / Burgold-Voigt, Sindy / Florou, Zoi / Braun, Sascha D / Monecke, Stefan / Gatselis, Nikolaos K / Zachou, Kalliopi / Stefos, Aggelos / Tsagalas, Ilias / Sofia, Marina / Spyrou, Vassiliki / Billinis, Charalambos / Dalekos, George N / Ehricht, Ralf / Petinaki, Efthymia

Microorganisms

2022 Volume 10, Issue 1

Abstract	Carbapenem-resistant Gram-negative bacteria are a public health threat that requires urgent action. The fact that these pathogens commonly also harbor resistance mechanisms for several other antimicrobial classes further reduces patient treatment options. The present study aimed to provide information regarding the multidrug resistance genetic background of carbapenem-resistant Gram-negative bacteria in Central Greece. Strains from a tertiary care hospital, collected during routine practice, were characterized using a DNA microarray-based assay. Various different resistance determinants for carbapenems, other beta-lactams, aminoglycosides, quinolones, trimethoprim, sulfonamides and macrolides were detected among isolates of the same sequence type. Eighteen different multidrug resistance genomic profiles were identified among the twenty-four
Language	English
Publishing date	2022-01-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10010159
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Soluble IL-2R Levels at Baseline Predict the Development of Severe Respiratory Failure and Mortality in COVID-19 Patients.

Gatselis, Nikolaos K / Lygoura, Vasiliki / Lyberopoulou, Aggeliki / Giannoulis, George / Samakidou, Anna / Vaiou, Antonia / Vatidis, George / Antoniou, Katerina / Stefos, Aggelos / Georgiadou, Sarah / Sagris, Dimitrios / Sveroni, Dafni / Stergioula, Despoina / Gabeta, Stella / Ntaios, George / Dalekos, George N

Viruses

2022 Volume 14, Issue 4

Abstract: Risk stratification of coronavirus disease-19 (COVID-19) patients by simple markers is critical to guide treatment. We studied the predictive value of soluble interleukin-2 receptor (sIL-2R) for the early identification of patients at risk of developing ... ...

Abstract	Risk stratification of coronavirus disease-19 (COVID-19) patients by simple markers is critical to guide treatment. We studied the predictive value of soluble interleukin-2 receptor (sIL-2R) for the early identification of patients at risk of developing severe clinical outcomes. sIL-2R levels were measured in 197 patients (60.9% males; median age 61 years; moderate disease,
MeSH term(s)	Biomarkers ; COVID-19/metabolism ; COVID-19/pathology ; Female ; Humans ; Male ; Middle Aged ; Predictive Value of Tests ; Receptors, Interleukin-2/blood ; Receptors, Interleukin-2/metabolism ; Respiratory Insufficiency/diagnosis ; Respiratory Insufficiency/metabolism
Chemical Substances	Biomarkers ; Receptors, Interleukin-2
Language	English
Publishing date	2022-04-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v14040787
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Lessons from pathophysiology: Use of individualized combination treatments with immune interventional agents to tackle severe respiratory failure in patients with COVID-19.

Dalekos, George N / Stefos, Aggelos / Georgiadou, Sarah / Lygoura, Vasiliki / Michail, Anastasia / Ntaios, George / Samakidou, Anna / Giannoulis, George / Gabeta, Stella / Vlychou, Marianna / Petinaki, Efthymia / Leventogiannis, Konstantinos / Giamarellos-Bourboulis, Evangelos J / Gatselis, Nikolaos K

European journal of internal medicine

2021 Volume 88, Page(s) 52–62

Abstract: Aims Infection by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) may lead to the development of severe respiratory failure. In hospitalized-patients, prompt interruption of the virus-driven inflammatory process by using combination ... ...

Abstract	Aims Infection by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) may lead to the development of severe respiratory failure. In hospitalized-patients, prompt interruption of the virus-driven inflammatory process by using combination treatments seems theoretically of outmost importance. Our aim was to investigate the hypothesis of multifaceted management of these patients. Methods A treatment algorithm based on ferritin was applied in 311 patients (67.2% males; median age 63-years; moderate disease, n=101; severe, n=210). Patients with ferritin <500ng/ml received anakinra 2-4mg/kg/day ± corticosteroids (Arm A, n=142) while those with ≥500ng/ml received anakinra 5-8mg/kg/day with corticosteroids and γ-globulins (Arm B, n=169). In case of no improvement a single dose of tocilizumab (8mg/kg; maximum 800mg) was administered with the potential of additional second and/or third pulses. Treatment endpoints were the rate of the development of respiratory failure necessitating intubation and the SARS-CoV-2-related mortality. The proposed algorithm was also validated in matched hospitalized-patients treated with standard-of-care during the same period. Results In overall, intubation and mortality rates were 5.8% and 5.1% (0% in moderate; 8.6% and 7.6% in severe). Low baseline pO
Language	English
Publishing date	2021-03-26
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1038679-8
ISSN	1879-0828 ; 0953-6205
ISSN (online)	1879-0828
ISSN	0953-6205
DOI	10.1016/j.ejim.2021.03.026
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2608: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Evaluation of rapid polymyxin NP test to detect colistin-resistant Klebsiella pneumoniae isolated in a tertiary Greek hospital.

Malli, Ergina / Florou, Zoi / Tsilipounidaki, Katerina / Voulgaridi, Ioanna / Stefos, Aggelos / Xitsas, Stelios / Papagiannitsis, Costas C / Petinaki, Efthimia

Journal of microbiological methods

2018 Volume 153, Page(s) 35–39

Abstract: The efficiency of Rapid Polymyxin NP test for detection of colistin-resistant isolates was tested against a collection of 131 non-repetitive Klebsiella pneumoniae, including 98 colistin-resistant and 33 colistin-susceptible isolates. In addition, the ... ...

Abstract	The efficiency of Rapid Polymyxin NP test for detection of colistin-resistant isolates was tested against a collection of 131 non-repetitive Klebsiella pneumoniae, including 98 colistin-resistant and 33 colistin-susceptible isolates. In addition, the performance of this test was compared with those of the automated systems, BD Phoenix™ and VITEK®2, and the Etest. Determination of imipenem and meropenem MICs showed that 95 of colistin-resistant (Col-R) isolates were also resistant to at least one carbapenem. Characterization of colistin resistance mechanisms showed that 75 out of 98 Col-R isolates were associated with the presence of alterations in the mgrB gene, while no mcr genes were detected among our isolates. Rapid Polymyxin NP correctly detected 97 out of 98 colistin-resistant isolates (Geometric mean MIC value 9.89 mg/L), except one ST147 K. pneumoniae harboring a wild-type mgrB gene (MIC: 8 mg/L), yielding a sensitivity 99%. The other methods gave more false-negative results with colistin-resistant strains; BD Phoenix™,VITEK®2, and the gradient Etest missed five, two and three colistin-resistant, respectively (95%, 98% and 97%). The Rapid Polymyxin NP test gave false positive results with six isolates, for which colistin MICs were 1-2 mg/L (specificity 82%). Despite the fact that Rapid Polymyxin exhibited lower specificity than other methods (82% versus 94%, 88% and 85%), it is easy-to-perform and rapid. Thus, these findings indicate that the Rapid Polymyxin NP test can be an initial tool for the detection of colistin-resistant isolates.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Automation, Laboratory ; Bacterial Proteins/genetics ; Carbapenems/pharmacology ; Colistin/pharmacology ; Disk Diffusion Antimicrobial Tests ; Drug Resistance, Bacterial/genetics ; False Positive Reactions ; Greece ; Humans ; Klebsiella Infections/microbiology ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/genetics ; Microbial Sensitivity Tests ; Polymyxins/pharmacology ; Sensitivity and Specificity ; Tertiary Care Centers ; beta-Lactamases/genetics
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; Carbapenems ; Polymyxins ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6) ; Colistin (Z67X93HJG1)
Language	English
Publishing date	2018-08-23
Publishing country	Netherlands
Document type	Comparative Study ; Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	604916-3
ISSN	1872-8359 ; 0167-7012
ISSN (online)	1872-8359
ISSN	0167-7012
DOI	10.1016/j.mimet.2018.08.010
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1849: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito