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  1. Article ; Online: SARS-CoV-2 biology and host interactions.

    Steiner, Silvio / Kratzel, Annika / Barut, G Tuba / Lang, Reto M / Aguiar Moreira, Etori / Thomann, Lisa / Kelly, Jenna N / Thiel, Volker

    Nature reviews. Microbiology

    2024  Volume 22, Issue 4, Page(s) 206–225

    Abstract: The zoonotic emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the ensuing coronavirus disease 2019 (COVID-19) pandemic have profoundly affected our society. The rapid spread and continuous evolution of new SARS-CoV-2 variants ...

    Abstract The zoonotic emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the ensuing coronavirus disease 2019 (COVID-19) pandemic have profoundly affected our society. The rapid spread and continuous evolution of new SARS-CoV-2 variants continue to threaten global public health. Recent scientific advances have dissected many of the molecular and cellular mechanisms involved in coronavirus infections, and large-scale screens have uncovered novel host-cell factors that are vitally important for the virus life cycle. In this Review, we provide an updated summary of the SARS-CoV-2 life cycle, gene function and virus-host interactions, including recent landmark findings on general aspects of coronavirus biology and newly discovered host factors necessary for virus replication.
    MeSH term(s) Humans ; SARS-CoV-2/genetics ; COVID-19 ; Virus Replication ; Biology
    Language English
    Publishing date 2024-01-15
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2139054-X
    ISSN 1740-1534 ; 1740-1526
    ISSN (online) 1740-1534
    ISSN 1740-1526
    DOI 10.1038/s41579-023-01003-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Proximity Labeling for the Identification of Coronavirus-Host Protein Interactions.

    V'kovski, Philip / Steiner, Silvio / Thiel, Volker

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 2203, Page(s) 187–204

    Abstract: Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein-protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the ... ...

    Abstract Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein-protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronavirus replicase/transcriptase complex (RTC), i.e., proteins located within a close perimeter of the RTC, can be determined by different proximity labeling approaches using BirA
    MeSH term(s) Animals ; Ascorbate Peroxidases/genetics ; Biotinylation ; Carbon-Nitrogen Ligases/genetics ; Cell Line ; Coronavirus/genetics ; Coronavirus/pathogenicity ; Enzymes/genetics ; Enzymes/metabolism ; Escherichia coli Proteins/genetics ; Fluorescent Antibody Technique ; Host-Pathogen Interactions/physiology ; Microorganisms, Genetically-Modified ; Proteomics/methods ; Repressor Proteins/genetics ; Viral Proteins/chemistry ; Viral Proteins/genetics ; Viral Proteins/metabolism
    Chemical Substances Enzymes ; Escherichia coli Proteins ; Repressor Proteins ; Viral Proteins ; Ascorbate Peroxidases (EC 1.11.1.11) ; Carbon-Nitrogen Ligases (EC 6.3.-) ; birA protein, E coli (EC 6.3.4.15)
    Keywords covid19
    Language English
    Publishing date 2020-07-29
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-0900-2_14
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: The Role of Stress Granules and the Nonsense-mediated mRNA Decay Pathway in Antiviral Defence.

    Contu, Lara / Steiner, Silvio / Thiel, Volker / Mühlemann, Oliver

    Chimia

    2019  Volume 73, Issue 5, Page(s) 374–379

    Abstract: Eukaryotic cells have evolved a number of survival tactics and quality control pathways that are able to counter intrinsic error-prone mechanisms and allow for maintenance of cellular homeostasis in the face of external stresses. This review will discuss ...

    Abstract Eukaryotic cells have evolved a number of survival tactics and quality control pathways that are able to counter intrinsic error-prone mechanisms and allow for maintenance of cellular homeostasis in the face of external stresses. This review will discuss the role of two cellular eukaryotic processes that are vital for maintenance of cellular homeostasis - 1) the nonsense-mediated mRNA decay (NMD) pathway and 2) the transient formation of stress granules (SG) - and explore the current literature on their roles in antiviral defence. Within the NCCR RNA & Disease, the laboratories of Proffs. O. Mühlemann and Volker Thiel teamed up to unravel the roles of NMD and SGs, and their interconnections in defending cells from alphavirus and coronavirus infections.
    MeSH term(s) Antiviral Agents ; Nonsense Mediated mRNA Decay ; RNA
    Chemical Substances Antiviral Agents ; RNA (63231-63-0)
    Keywords covid19
    Language English
    Publishing date 2019-05-08
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 1516-7
    ISSN 0009-4293
    ISSN 0009-4293
    DOI 10.2533/chimia.2019.374
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Proximity Labeling for the Identification of Coronavirus-Host Protein Interactions

    V039, / kovski, Philip / Steiner, Silvio / Thiel, Volker

    Methods Mol Biol

    Abstract: Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein-protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the ... ...

    Abstract Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein-protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronavirus replicase/transcriptase complex (RTC), i.e., proteins located within a close perimeter of the RTC, can be determined by different proximity labeling approaches using BirAR118G (BioID), TurboID, and APEX2. These factors represent a molecular signature of coronavirus RTCs and likely contribute to the viral life cycle, thereby constituting attractive targets for the development of antiviral intervention strategies.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #761353
    Database COVID19

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  5. Article ; Online: Coronavirus biology and replication: implications for SARS-CoV-2.

    V'kovski, Philip / Kratzel, Annika / Steiner, Silvio / Stalder, Hanspeter / Thiel, Volker

    Nature reviews. Microbiology

    2020  Volume 19, Issue 3, Page(s) 155–170

    Abstract: The SARS-CoV-2 pandemic and its unprecedented global societal and economic disruptive impact has marked the third zoonotic introduction of a highly pathogenic coronavirus into the human population. Although the previous coronavirus SARS-CoV and MERS-CoV ... ...

    Abstract The SARS-CoV-2 pandemic and its unprecedented global societal and economic disruptive impact has marked the third zoonotic introduction of a highly pathogenic coronavirus into the human population. Although the previous coronavirus SARS-CoV and MERS-CoV epidemics raised awareness of the need for clinically available therapeutic or preventive interventions, to date, no treatments with proven efficacy are available. The development of effective intervention strategies relies on the knowledge of molecular and cellular mechanisms of coronavirus infections, which highlights the significance of studying virus-host interactions at the molecular level to identify targets for antiviral intervention and to elucidate critical viral and host determinants that are decisive for the development of severe disease. In this Review, we summarize the first discoveries that shape our current understanding of SARS-CoV-2 infection throughout the intracellular viral life cycle and relate that to our knowledge of coronavirus biology. The elucidation of similarities and differences between SARS-CoV-2 and other coronaviruses will support future preparedness and strategies to combat coronavirus infections.
    MeSH term(s) Animals ; COVID-19/virology ; Host-Pathogen Interactions ; Humans ; SARS-CoV-2/chemistry ; SARS-CoV-2/physiology ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Virus Internalization ; Virus Replication ; COVID-19 Drug Treatment
    Chemical Substances Viral Proteins
    Keywords covid19
    Language English
    Publishing date 2020-10-28
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2139054-X
    ISSN 1740-1534 ; 1740-1526
    ISSN (online) 1740-1534
    ISSN 1740-1526
    DOI 10.1038/s41579-020-00468-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Temperature-dependent surface stability of SARS-CoV-2.

    Kratzel, Annika / Steiner, Silvio / Todt, Daniel / V'kovski, Philip / Brueggemann, Yannick / Steinmann, Joerg / Steinmann, Eike / Thiel, Volker / Pfaender, Stephanie

    The Journal of infection

    2020  Volume 81, Issue 3, Page(s) 452–482

    MeSH term(s) Betacoronavirus ; COVID-19 ; Coronavirus Infections/epidemiology ; Humans ; Pandemics ; Pneumonia, Viral/epidemiology ; SARS-CoV-2 ; Temperature
    Keywords covid19
    Language English
    Publishing date 2020-06-03
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2020.05.074
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  7. Article: Coronavirus biology and replication: implications for SARS-CoV-2

    V039, / kovski, Philip / Kratzel, Annika / Steiner, Silvio / Stalder, Hanspeter / Thiel, Volker

    Nat. rev. microbiol

    Abstract: The SARS-CoV-2 pandemic and its unprecedented global societal and economic disruptive impact has marked the third zoonotic introduction of a highly pathogenic coronavirus into the human population. Although the previous coronavirus SARS-CoV and MERS-CoV ... ...

    Abstract The SARS-CoV-2 pandemic and its unprecedented global societal and economic disruptive impact has marked the third zoonotic introduction of a highly pathogenic coronavirus into the human population. Although the previous coronavirus SARS-CoV and MERS-CoV epidemics raised awareness of the need for clinically available therapeutic or preventive interventions, to date, no treatments with proven efficacy are available. The development of effective intervention strategies relies on the knowledge of molecular and cellular mechanisms of coronavirus infections, which highlights the significance of studying virus-host interactions at the molecular level to identify targets for antiviral intervention and to elucidate critical viral and host determinants that are decisive for the development of severe disease. In this Review, we summarize the first discoveries that shape our current understanding of SARS-CoV-2 infection throughout the intracellular viral life cycle and relate that to our knowledge of coronavirus biology. The elucidation of similarities and differences between SARS-CoV-2 and other coronaviruses will support future preparedness and strategies to combat coronavirus infections.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #894400
    Database COVID19

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  8. Article ; Online: Author Correction: Rhinovirus-induced epithelial RIG-I inflammasome suppresses antiviral immunity and promotes inflammation in asthma and COVID-19.

    Radzikowska, Urszula / Eljaszewicz, Andrzej / Tan, Ge / Stocker, Nino / Heider, Anja / Westermann, Patrick / Steiner, Silvio / Dreher, Anita / Wawrzyniak, Paulina / Rückert, Beate / Rodriguez-Coira, Juan / Zhakparov, Damir / Huang, Mengting / Jakiela, Bogdan / Sanak, Marek / Moniuszko, Marcin / O'Mahony, Liam / Jutel, Marek / Kebadze, Tatiana /
    Jackson, David J / Edwards, Michael R / Thiel, Volker / Johnston, Sebastian L / Akdis, Cezmi A / Sokolowska, Milena

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 3493

    Language English
    Publishing date 2023-06-13
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-39275-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Rhinovirus-induced epithelial RIG-I inflammasome suppresses antiviral immunity and promotes inflammation in asthma and COVID-19.

    Radzikowska, Urszula / Eljaszewicz, Andrzej / Tan, Ge / Stocker, Nino / Heider, Anja / Westermann, Patrick / Steiner, Silvio / Dreher, Anita / Wawrzyniak, Paulina / Rückert, Beate / Rodriguez-Coira, Juan / Zhakparov, Damir / Huang, Mengting / Jakiela, Bogdan / Sanak, Marek / Moniuszko, Marcin / O'Mahony, Liam / Jutel, Marek / Kebadze, Tatiana /
    Jackson, David J / Edwards, Michael R / Thiel, Volker / Johnston, Sebastian L / Akdis, Cezmi A / Sokolowska, Milena

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 2329

    Abstract: Rhinoviruses and allergens, such as house dust mite are major agents responsible for asthma exacerbations. The influence of pre-existing airway inflammation on the infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely ... ...

    Abstract Rhinoviruses and allergens, such as house dust mite are major agents responsible for asthma exacerbations. The influence of pre-existing airway inflammation on the infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely unknown. We analyse mechanisms of response to viral infection in experimental in vivo rhinovirus infection in healthy controls and patients with asthma, and in in vitro experiments with house dust mite, rhinovirus and SARS-CoV-2 in human primary airway epithelium. Here, we show that rhinovirus infection in patients with asthma leads to an excessive RIG-I inflammasome activation, which diminishes its accessibility for type I/III interferon responses, leading to their early functional impairment, delayed resolution, prolonged viral clearance and unresolved inflammation in vitro and in vivo. Pre-exposure to house dust mite augments this phenomenon by inflammasome priming and auxiliary inhibition of early type I/III interferon responses. Prior infection with rhinovirus followed by SARS-CoV-2 infection augments RIG-I inflammasome activation and epithelial inflammation. Timely inhibition of the epithelial RIG-I inflammasome may lead to more efficient viral clearance and lower the burden of rhinovirus and SARS-CoV-2 infections.
    MeSH term(s) Humans ; Antiviral Restriction Factors/genetics ; Antiviral Restriction Factors/metabolism ; Asthma/genetics ; Asthma/immunology ; COVID-19/genetics ; COVID-19/immunology ; DEAD Box Protein 58/metabolism ; Enterovirus Infections/genetics ; Enterovirus Infections/immunology ; Inflammasomes/genetics ; Inflammasomes/metabolism ; Inflammation ; Interferon Type I ; Picornaviridae Infections/genetics ; Picornaviridae Infections/immunology ; Rhinovirus/metabolism ; Rhinovirus/pathogenicity ; SARS-CoV-2
    Chemical Substances Antiviral Restriction Factors ; DEAD Box Protein 58 (EC 3.6.4.13) ; Inflammasomes ; Interferon Type I
    Language English
    Publishing date 2023-04-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-37470-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Temperature-dependent surface stability of SARS-CoV-2

    Kratzel, Annika / Steiner, Silvio / Todt, Daniel / V039, / kovski, Philip / Brueggemann, Yannick / Steinmann, Joerg / Steinmann, Eike / Thiel, Volker / Pfaender, Stephanie

    J Infect

    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #505673
    Database COVID19

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