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  1. Article ; Online: Bacitracin agar vs. oleandomycin disk supplemented chocolate agar for the recovery of Haemophilus influenzae in diagnostic samples: A prospective comparison.

    Forstner, Patrick / Laireiter, Christina Maria / Friedl, Simone / Steinmetz, Ivo / Dichtl, Karl

    Diagnostic microbiology and infectious disease

    2024  Volume 109, Issue 1, Page(s) 116203

    Abstract: Haemophilus influenzae is an important pathogen able to cause various forms of respiratory and invasive disease. To provide high sensitivity for detection, culture media must inhibit growth of residential flora from the respiratory tract. This study ... ...

    Abstract Haemophilus influenzae is an important pathogen able to cause various forms of respiratory and invasive disease. To provide high sensitivity for detection, culture media must inhibit growth of residential flora from the respiratory tract. This study aimed to identify and compare the diagnostic and economic advantages of using bacitracin containing selective agar (SEL) or oleandomycin disk supplemented chocolate agar (CHOC). Growth and semi-quantitative abundance of H. influenzae and growth suppression of residential flora was prospectively assessed in a 28-week period. H. influenzae was identified in 164 (5 %) of all included samples: CHOC and SEL, CHOC only, and SEL only were positive in 95, 24, and 45 cases. Diagnostic superiority of SEL was primarily attributable to the results of throat swabs. However, on average, € 200 had to be spent for the detection of each additional isolate that was recovered only because of additional incubation on SEL.
    MeSH term(s) Humans ; Agar ; Bacitracin/pharmacology ; Haemophilus influenzae ; Oleandomycin ; Chocolate ; Culture Media
    Chemical Substances Agar (9002-18-0) ; Bacitracin (1405-87-4) ; Oleandomycin (P8ZQ646136) ; Culture Media
    Language English
    Publishing date 2024-02-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2024.116203
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    Zs.A 1845: Show issues Location:
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  2. Book ; Thesis: Herstellung und Charakterisierung von syngenen monoklonalen Anti-idiotypischen Antikörpern gegen monoklonale Anti-C3a-Antikörper

    Steinmetz, Ivo

    1989  

    Author's details vorgelegt von Ivo Steinmetz
    Language German
    Size 2 Mikrofiches : 24x
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Mainz, Univ., Diss., 1989
    Note Mikroreprod. e. Ms. Getr. Zählung: graph. Darst.
    HBZ-ID HT003656017
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    1990 MB 194 order for loan Magazin

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  3. Article ; Online: Complete Genome Sequence of Klebsiella oxytoca Strain AHC-6, Isolated from a Patient during Acute Antibiotic-Associated Hemorrhagic Colitis.

    Dabernig-Heinz, Johanna / Wagner, Gabriel E / Leitner, Eva / Ruppitsch, Werner / Steinmetz, Ivo / Högenauer, Christoph / Zechner, Ellen L / Kienesberger, Sabine

    Microbiology resource announcements

    2023  Volume 12, Issue 4, Page(s) e0135022

    Abstract: Klebsiella oxytoca is a ubiquitous bacterium that is increasingly associated with inflammatory diseases. Here, we report the hybrid assembled genome for cytotoxic K. oxytoca strain AHC-6. The genome comprises a total of 5.7 Mbp, with a GC content of 55.2% ...

    Abstract Klebsiella oxytoca is a ubiquitous bacterium that is increasingly associated with inflammatory diseases. Here, we report the hybrid assembled genome for cytotoxic K. oxytoca strain AHC-6. The genome comprises a total of 5.7 Mbp, with a GC content of 55.2% and 5,258 coding sequences after assembly and annotation.
    Language English
    Publishing date 2023-03-16
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/mra.01350-22
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  4. Article: Rational design of an acidic erythritol (ACER) medium for the enhanced isolation of the environmental pathogen

    Assig, Karoline / Lichtenegger, Sabine / Bui, Linh N H / Mosbacher, Bettina / Vu, Anh T N / Erhart, Daniel / Trinh, Trung T / Steinmetz, Ivo

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1213818

    Abstract: ... The soil ... ...

    Abstract The soil bacterium
    Language English
    Publishing date 2023-06-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1213818
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  5. Article ; Online: A head-to-head comparison of three MALDI-TOF mass spectrometry systems with 16S rRNA gene sequencing.

    Dichtl, Karl / Klugherz, Isabel / Greimel, Hanna / Luxner, Josefa / Köberl, Julian / Friedl, Simone / Steinmetz, Ivo / Leitner, Eva

    Journal of clinical microbiology

    2023  Volume 61, Issue 10, Page(s) e0191322

    Abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized diagnostics in culture-based microbiology. Commonly used MALDI-TOF MS systems in clinical microbiology laboratories are MALDI Biotyper (Bruker ... ...

    Abstract Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized diagnostics in culture-based microbiology. Commonly used MALDI-TOF MS systems in clinical microbiology laboratories are MALDI Biotyper (Bruker Daltonics) and Vitek MS (bioMérieux), but recently the new EXS2600 (Zybio) has been launched. This study aimed to evaluate the performance of the three devices by comparing the results to 16S rRNA gene sequencing. A set of 356 previously collected difficult-to-identify bacteria was tested in parallel with the three systems. Only the direct smear method and simple formic acid extraction were applied. Valid results were achieved for 98.6%, 94.4%, and 93.3% of all isolates by MALDI Biotyper, EXS2600, and Vitek MS, respectively. Of all valid results, agreement with sequencing data was achieved in 98.9%, 98.5%, and 99.7% by MALDI Biotyper, EXS2600, and Vitek MS, respectively. Considering only the isolates with valid measurements at the single-species level, misidentification rates were 0%, 2.6%, and 1.1% for MALDI Biotyper, EXS2600, and Vitek MS, respectively. Apart from minor performance differences, our data demonstrate that the three systems provide comparable results and are suitable for use in medical diagnostic laboratories.
    MeSH term(s) Humans ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; RNA, Ribosomal, 16S/genetics ; Genes, rRNA ; Bacteria ; Clinical Laboratory Services
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2023-09-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/jcm.01913-22
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    Zs.A 1188: Show issues Location:
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  6. Article ; Online: Establishing breakpoints for amoxicillin/clavulanate and ampicillin/sulbactam for rapid antimicrobial susceptibility testing directly from positive blood culture bottles.

    König, Elisabeth / Iser, Juliane / Zechner-Sammer, Tanja / Friedl, Herwig / Krause, Robert / Valentin, Thomas / Leitner, Eva / Steinmetz, Ivo / Zollner-Schwetz, Ines

    Journal of medical microbiology

    2023  Volume 72, Issue 3

    Abstract: Introduction. ...

    Abstract Introduction.
    MeSH term(s) Sulbactam/pharmacology ; Blood Culture ; Escherichia coli ; Ampicillin/pharmacology ; Amoxicillin-Potassium Clavulanate Combination/pharmacology ; Anti-Infective Agents ; Anti-Bacterial Agents/pharmacology ; Microbial Sensitivity Tests
    Chemical Substances sultamicillin (65DT0ML581) ; Sulbactam (S4TF6I2330) ; Ampicillin (7C782967RD) ; Amoxicillin-Potassium Clavulanate Combination (74469-00-4) ; Anti-Infective Agents ; Anti-Bacterial Agents
    Language English
    Publishing date 2023-03-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 218356-0
    ISSN 1473-5644 ; 0022-2615
    ISSN (online) 1473-5644
    ISSN 0022-2615
    DOI 10.1099/jmm.0.001672
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    Zs.A 634: Show issues Location:
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  7. Article ; Online: Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison.

    Desoutter, Anais / Deshayes, Thomas / Vorimore, Fabien / Klotoe, Bernice / Durand, Benoit / Colot, Julien / Wagner-Lichtenegger, Gabriel / Steinmetz, Ivo / Tuanyok, Apichai / Laroucau, Karine

    BMC veterinary research

    2024  Volume 20, Issue 1, Page(s) 114

    Abstract: Background: Melioidosis is a serious bacterial infection caused by Burkholderia pseudomallei, a gram-negative bacterium commonly found in soil and water. It can affect both humans and animals, and is endemic in regions such as Southeast Asia and ... ...

    Abstract Background: Melioidosis is a serious bacterial infection caused by Burkholderia pseudomallei, a gram-negative bacterium commonly found in soil and water. It can affect both humans and animals, and is endemic in regions such as Southeast Asia and Northern Australia. In recent years, there have been reports of an emergence of human melioidosis in other areas, including New Caledonia.
    Results: During standard laboratory analysis in New Caledonia in 2021, a strain of B. pseudomallei was isolated from a goat. The strain was characterized using both MLST and WGS techniques and was found to cluster with previously described local human strains from the area. In parallel, several serological tests (CFT, ELISA, Luminex (Hcp1, GroEL, BPSS1840), arrays assay and a latex agglutination test) were performed on animals from the farm where the goat originated, and/or from three other neighboring farms. Using two commercial ELISA kits, seropositive animals were found only on the farm where the infected goat originated and tests based on recombinant proteins confirmed the usefulness of the Hcp1 protein for the diagnosis of melioidosis in animals.
    Conclusions: Despite the regular reports of human cases, this is the first confirmed case of melioidosis in an animal in New Caledonia. These results confirm the presence of the bacterium in the region and highlight the importance of vigilance for both animal and human health. It is critical that all health partners, including breeders, veterinarians, and biologists, work together to monitor and prevent the spread of the disease.
    MeSH term(s) Humans ; Animals ; Burkholderia pseudomallei/genetics ; Melioidosis/diagnosis ; Melioidosis/epidemiology ; Melioidosis/veterinary ; Multilocus Sequence Typing/veterinary ; Goats ; New Caledonia/epidemiology ; Goat Diseases
    Language English
    Publishing date 2024-03-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 2191675-5
    ISSN 1746-6148 ; 1746-6148
    ISSN (online) 1746-6148
    ISSN 1746-6148
    DOI 10.1186/s12917-024-03957-5
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  8. Article ; Online: Aktuelle Epidemiologie von Bordetella parapertussis-Infektionen in Deutschland

    Schönfeld, Viktoria / Heininger, Ulrich / Littmann, Martina / Steinmetz, Ivo / Matysiak-Klose, Dorothea

    2023  

    Abstract: Keuchhusten wird in den meisten Fällen durch Bordetella pertussis (B. pertussis) verursacht, seltener auch durch B. parapertussis. Seit 2013 besteht gemäß § 6 Infektionsschutzgesetz (IfSG) eine bundesweite Meldepflicht für den Krankheitsverdacht, die ... ...

    Abstract Keuchhusten wird in den meisten Fällen durch Bordetella pertussis (B. pertussis) verursacht, seltener auch durch B. parapertussis. Seit 2013 besteht gemäß § 6 Infektionsschutzgesetz (IfSG) eine bundesweite Meldepflicht für den Krankheitsverdacht, die Erkrankung und den Tod durch B. pertussis oder B. parapertussis (sog. Arztmeldepflicht) sowie für den direkten Nachweis der beiden Erreger und den indirekten Nachweis von B. pertussis (§ 7 IfSG, sog. Labormeldepflicht). Im Zuge der COVID-19-Pandemie und den damit verbundenen Infektionsschutzmaßnahmen war ein deutlicher Rückgang der Keuchhustenfallzahlen in Deutschland zu beobachten. Während die B. per-tussis-Fallzahlen derzeit weiterhin unter dem präpandemischen Niveau verbleiben, lässt sich seit dem vierten Quartal 2022 eine deutliche Zunahme von B. parapertussis-Erkrankungen beobachten, die die präpandemischen Fallzahlen übertrifft. Ursachen hierfür sind wahrscheinlich u. a. ein Nachholeffekt nach der COVID-19-Pandemie, ein verändertes Diagnostikverhalten und das Fehlen einer ausreichend wirksamen Impfung.

    Peer Reviewed
    Keywords Keuchhusten ; Bordetella parapertussis ; Meldepflicht ; IfSG ; Epidemiologie ; 610 Medizin und Gesundheit ; ddc:610
    Language German
    Publishing date 2023-08-17
    Publisher Robert Koch-Institut
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article: Environmental Factors Associated With Soil Prevalence of the Melioidosis Pathogen

    Shaw, Tushar / Assig, Karoline / Tellapragada, Chaitanya / Wagner, Gabriel E / Choudhary, Madhu / Göhler, André / Eshwara, Vandana Kalwaje / Steinmetz, Ivo / Mukhopadhyay, Chiranjay

    Frontiers in microbiology

    2022  Volume 13, Page(s) 902996

    Abstract: Melioidosis is a seasonal infectious disease in tropical and subtropical areas caused by the soil ... ...

    Abstract Melioidosis is a seasonal infectious disease in tropical and subtropical areas caused by the soil bacterium
    Language English
    Publishing date 2022-07-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.902996
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  10. Article: Open-Source, Adaptable, All-in-One Smartphone-Based System for Quantitative Analysis of Point-of-Care Diagnostics.

    Schary, Weronika / Paskali, Filip / Rentschler, Simone / Ruppert, Christoph / Wagner, Gabriel E / Steinmetz, Ivo / Deigner, Hans-Peter / Kohl, Matthias

    Diagnostics (Basel, Switzerland)

    2022  Volume 12, Issue 3

    Abstract: Point-of-care (POC) diagnostics, in particular lateral flow assays (LFA), represent a great opportunity for rapid, precise, low-cost and accessible diagnosis of disease. Especially with the ongoing coronavirus disease 2019 (COVID-19) pandemic, rapid ... ...

    Abstract Point-of-care (POC) diagnostics, in particular lateral flow assays (LFA), represent a great opportunity for rapid, precise, low-cost and accessible diagnosis of disease. Especially with the ongoing coronavirus disease 2019 (COVID-19) pandemic, rapid point-of-care tests are becoming everyday tools for identification and prevention. Using smartphones as biosensors can enhance POC devices as portable, low-cost platforms for healthcare and medicine, food and environmental monitoring, improving diagnosis and documentation in remote, low-resource locations. We present an open-source, all-in-one smartphone-based system for quantitative analysis of LFAs. It consists of a 3D-printed photo box, a smartphone for image acquisition, and an R Shiny software package with modular, customizable analysis workflow for image editing, analysis, data extraction, calibration and quantification of the assays. This system is less expensive than commonly used hardware and software, so it could prove very beneficial for diagnostic testing in the context of pandemics, as well as in low-resource countries.
    Language English
    Publishing date 2022-02-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics12030589
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