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  1. Article ; Online: Molecular Pathology, Diagnostics and Therapeutics

    Stephen A. Bustin

    International Journal of Molecular Sciences, Vol 24, Iss 5063, p

    A Story of Success in 2022

    2023  Volume 5063

    Abstract: Molecular pathology, diagnostics and therapeutics are three closely related topics of critical importance in medical research and clinical practice [.] ...

    Abstract Molecular pathology, diagnostics and therapeutics are three closely related topics of critical importance in medical research and clinical practice [.]
    Keywords n/a ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Advances in Molecular Medicine

    Stephen A. Bustin / Kurt A. Jellinger

    International Journal of Molecular Sciences, Vol 24, Iss 14168, p

    Unravelling Disease Complexity and Pioneering Precision Healthcare

    2023  Volume 14168

    Abstract: The escalating impacts of the climate crisis, zoonotic spill-over, and antibiotic resistance have positioned molecular medicine at the forefront of pioneering translational research [.] ...

    Abstract The escalating impacts of the climate crisis, zoonotic spill-over, and antibiotic resistance have positioned molecular medicine at the forefront of pioneering translational research [.]
    Keywords n/a ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2023-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Book ; Online: RT-qPCR Testing of SARS-CoV-2

    Stephen A. Bustin / Tania Nolan

    International Journal of Molecular Sciences ; Volume 21 ; Issue 8

    A Primer

    2020  

    Abstract: Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely ... ...

    Abstract Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR.
    Keywords COVID-19 ; SARS ; pandemic ; reverse transcription ; real-time fluorescence PCR ; covid19
    Language English
    Publishing date 2020-04-24
    Publisher Multidisciplinary Digital Publishing Institute
    Publishing country ch
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: RT-qPCR Testing of SARS-CoV-2

    Stephen A. Bustin / Tania Nolan

    International Journal of Molecular Sciences, Vol 21, Iss 3004, p

    A Primer

    2020  Volume 3004

    Abstract: Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely ... ...

    Abstract Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR.
    Keywords COVID-19 ; SARS ; pandemic ; reverse transcription ; real-time fluorescence PCR ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2020-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Reproducibility of biomedical research – The importance of editorial vigilance

    Stephen A. Bustin

    Biomolecular Detection and Quantification, Vol 11, Iss C, Pp 1-

    2017  Volume 3

    Abstract: Many journal editors are a failing to implement their own authors’ instructions, resulting in the publication of many articles that do not meet basic standards of transparency, employ unsuitable data analysis methods and report overly optimistic ... ...

    Abstract Many journal editors are a failing to implement their own authors’ instructions, resulting in the publication of many articles that do not meet basic standards of transparency, employ unsuitable data analysis methods and report overly optimistic conclusions. This problem is particularly acute where quantitative measurements are made and results in the publication of papers that lack scientific rigor and contributes to the concerns with regard to the reproducibility of biomedical research. This hampers research areas such as biomarker identification, as reproducing all but the most striking changes is challenging and translation to patient care rare.
    Keywords Biology (General) ; QH301-705.5 ; Microbiology ; QR1-502
    Language English
    Publishing date 2017-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: How to speed up the polymerase chain reaction

    Stephen A. Bustin

    Biomolecular Detection and Quantification, Vol 12, Iss C, Pp 10-

    2017  Volume 14

    Abstract: Reducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce ...

    Abstract Reducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.
    Keywords Real-time PCR ; Taq polymerase ; Denaturation ; Annealing ; Polymerisation ; Biology (General) ; QH301-705.5 ; Microbiology ; QR1-502
    Language English
    Publishing date 2017-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Improving the reliability of peer-reviewed publications

    Stephen A. Bustin

    Biomolecular Detection and Quantification, Vol 7, Iss C, Pp A1-A

    We are all in it together

    2016  Volume 5

    Abstract: The current, and welcome, focus on standardization of techniques and transparency of reporting in the biomedical, peer-reviewed literature is commendable. However, that focus has been intermittent as well as lacklustre and so failed to tackle the ... ...

    Abstract The current, and welcome, focus on standardization of techniques and transparency of reporting in the biomedical, peer-reviewed literature is commendable. However, that focus has been intermittent as well as lacklustre and so failed to tackle the alarming lack of reliability and reproducibly of biomedical research. Authors have access to numerous recommendations, ranging from simple standards dealing with technical issues to those regulating clinical trials, suggesting that improved reporting guidelines are not the solution. The elemental solution is for editors to require meticulous implementation of their journals’ instructions for authors and reviewers and stipulate that no paper is published without a transparent, complete and accurate materials and methods section.
    Keywords Research ; Reproducibility ; Biomedicine ; qPCR ; Microarrays ; Next generation sequencing ; Biology (General) ; QH301-705.5 ; Microbiology ; QR1-502
    Language English
    Publishing date 2016-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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  8. Article ; Online: RT-qPCR Detection of SARS-CoV-2

    Stephen A. Bustin / Gregory L. Shipley / Sara Kirvell / Reinhold Mueller / Tania Nolan

    International Journal of Molecular Sciences, Vol 23, Iss 1303, p

    No Need for a Dedicated Reverse Transcription Step

    2022  Volume 1303

    Abstract: Reverse transcription of RNA coupled to amplification of the resulting cDNA by the polymerase chain reaction (RT-PCR) is one of the principal molecular technologies in use today, with applications across all areas of science and medicine. In its real- ... ...

    Abstract Reverse transcription of RNA coupled to amplification of the resulting cDNA by the polymerase chain reaction (RT-PCR) is one of the principal molecular technologies in use today, with applications across all areas of science and medicine. In its real-time, fluorescence-based usage (RT-qPCR), it has long been a core technology driving the accurate, rapid and sensitive laboratory diagnosis of infectious diseases. However, RT-qPCR protocols have changed little over the past 30 years, with the RT step constituting a significant percentage of the time taken to complete a typical RT-qPCR assay. When applied to research investigations, reverse transcription has been evaluated by criteria such as maximum yield, length of transcription, fidelity, and faithful representation of an RNA pool. Crucially, however, these are of less relevance in a diagnostic RT-PCR test, where speed and sensitivity are the prime RT imperatives, with specificity contributed by the PCR component. We propose a paradigm shift that omits the requirement for a separate high-temperature RT step at the beginning of an RT-qPCR assay. This is achieved by means of an innovative protocol that incorporates suitable reagents with a revised primer and amplicon design and we demonstrate a proof of principle that incorporates the RT step as part of the PCR assay setup at room temperature. Use of this modification as part of a diagnostic assay will of course require additional characterisation, validation and optimisation of the PCR step. Combining this revision with our previous development of fast qPCR protocols allows completion of a 40 cycle RT-qPCR run on a suitable commercial instrument in approximately 15 min. Even faster times, in combination with extreme PCR procedures, can be achieved.
    Keywords reverse transcription ; RNA ; molecular diagnostics ; RT-qPCR ; SARS-CoV-2 ; COVID-19 ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 621
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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  9. Article ; Online: The reproducibility of biomedical research

    Stephen A. Bustin

    Biomolecular Detection and Quantification, Vol 2, Iss C, Pp 35-

    Sleepers awake!

    2014  Volume 42

    Abstract: There is increasing concern about the reliability of biomedical research, with recent articles suggesting that up to 85% of research funding is wasted. This article argues that an important reason for this is the inappropriate use of molecular techniques, ...

    Abstract There is increasing concern about the reliability of biomedical research, with recent articles suggesting that up to 85% of research funding is wasted. This article argues that an important reason for this is the inappropriate use of molecular techniques, particularly in the field of RNA biomarkers, coupled with a tendency to exaggerate the importance of research findings.
    Keywords Reproducibility ; Biomedicine ; qPCR ; Microarrays ; Cancer ; Next generation sequencing ; Biology (General) ; QH301-705.5 ; Microbiology ; QR1-502
    Language English
    Publishing date 2014-12-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: International Journal of Molecular Science Best Paper Award 2014

    Stephen A. Bustin / Ian A. Nicholls / Michael Iba

    International Journal of Molecular Sciences, Vol 15, Iss 1, Pp 1683-

    2014  Volume 1685

    Abstract: International Journal of Molecular Science is instituting an annual award to recognize outstanding papers in the area of chemistry, molecular physics and molecular biology published in International Journal of Molecular Science. We are pleased to ... ...

    Abstract International Journal of Molecular Science is instituting an annual award to recognize outstanding papers in the area of chemistry, molecular physics and molecular biology published in International Journal of Molecular Science. We are pleased to announce the third “International Journal of Molecular Science Best Paper Award” for 2014 [1,2]. Nominations were made by the Section Editors-in-Chief of International Journal of Molecular Science from all papers published in 2010.
    Keywords n/a ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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