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Article: Determination of Bile Acids in Canine Biological Samples: Diagnostic Significance.

Németh, Krisztián / Sterczer, Ágnes / Kiss, Dávid Sándor / Lányi, Réka Katalin / Hemző, Vivien / Vámos, Kriszta / Bartha, Tibor / Buzás, Anna / Lányi, Katalin

Metabolites

2024 Volume 14, Issue 4

Abstract: The comprehensive examination of bile acids is of paramount importance across various fields of health sciences, influencing physiology, microbiology, internal medicine, and pharmacology. While enzymatic reaction-based photometric methods remain ... ...

Abstract	The comprehensive examination of bile acids is of paramount importance across various fields of health sciences, influencing physiology, microbiology, internal medicine, and pharmacology. While enzymatic reaction-based photometric methods remain fundamental for total BA measurements, there is a burgeoning demand for more sophisticated techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) for comprehensive BA profiling. This evolution reflects a need for nuanced diagnostic assessments in clinical practice. In canines, a BA assessment involves considering factors, such as food composition, transit times, and breed-specific variations. Multiple matrices, including blood, feces, urine, liver tissue, and gallbladder bile, offer insights into BA profiles, yet interpretations remain complex, particularly in fecal analysis due to sampling challenges and breed-specific differences. Despite ongoing efforts, a consensus regarding optimal matrices and diagnostic thresholds remains elusive, highlighting the need for further research. Emphasizing the scarcity of systematic animal studies and underscoring the importance of ap-propriate sampling methodologies, our review advocates for targeted investigations into BA alterations in canine pathology, promising insights into pathomechanisms, early disease detection, and therapeutic avenues.
Language	English
Publishing date	2024-03-22
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662251-8
ISSN	2218-1989
ISSN	2218-1989
DOI	10.3390/metabo14040178
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Investigation of the environmental presence of multidrug-resistant bacteria at small animal hospitals in Hungary.

Kerek, Ádám / Sterczer, Ágnes / Somogyi, Zoltán / Kovács, Dóra / Jerzsele, Ákos

Acta veterinaria Hungarica

2021 Volume 68, Issue 4, Page(s) 387–392

Abstract: Multidrug-resistant bacteria can cause severe nosocomial infections in both human and veterinary clinics. The aim of this study was to investigate the presence and antibiotic susceptibility of Enterococcus, Staphylococcus and Pseudomonas strains at four ... ...

Abstract	Multidrug-resistant bacteria can cause severe nosocomial infections in both human and veterinary clinics. The aim of this study was to investigate the presence and antibiotic susceptibility of Enterococcus, Staphylococcus and Pseudomonas strains at four small animal clinics of Hungary in 2018, as these bacteria can reliably represent the level of antimicrobial resistance in the investigated environment. A total of 177 Staphylococcus colonies were found, including 22 Staphylococcus pseudintermedius and 13 Staphylococcus aureus. As regards enterococci, 9 Enterococcus faecium, 2 E. faecalis and further 286 Enterococcus strains were isolated. The number of Pseudomonas aeruginosa isolates (n = 34) was considered too low for relevant susceptibility testing. Among staphylococci, the highest resistance was found to sulphamethoxazole (82.9%), penicillin (65.7%) and erythromycin (54.3%), while in the case of enterococci, resistance to norfloxacin and rifampicin was the most common, with 25.5% of the strains being resistant to both antibiotics. Ten methicillin-resistant S. pseudintermedius (MRSP) and six vancomycin-resistant Enterococcus (VRE) strains could be identified. Only 5.7% of the Staphylococcus isolates were susceptible to all tested agents, while this ratio was 36.2% among enterococci. The results of this study have revealed a high prevalence of antibiotic-resistant bacteria in Hungarian small animal clinics, which highlights the importance of regular disinfection processes and stringent hygiene measures in veterinary clinics.
MeSH term(s)	Animals ; Anti-Bacterial Agents/pharmacology ; Hospitals, Animal ; Hungary ; Microbial Sensitivity Tests/veterinary ; Staphylococcus ; Vancomycin-Resistant Enterococci
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2021-01-05
Publishing country	Hungary
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605742-1
ISSN	1588-2705 ; 0236-6290 ; 0365-8198
ISSN (online)	1588-2705
ISSN	0236-6290 ; 0365-8198
DOI	10.1556/004.2020.00055
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Article ; Online: Altered Intestinal Production of Volatile Fatty Acids in Dogs Triggered by Lactulose and Psyllium Treatment.

Mackei, Máté / Talabér, Rebeka / Müller, Linda / Sterczer, Ágnes / Fébel, Hedvig / Neogrády, Zsuzsanna / Mátis, Gábor

Veterinary sciences

2022 Volume 9, Issue 5

Abstract: The intestinal microbiome of dogs can be influenced by a number of factors such as non-starch polysaccharides as well as some non-digestible oligo- and disaccharides. These molecules are only decomposed by intestinal anaerobic microbial fermentation, ... ...

Abstract	The intestinal microbiome of dogs can be influenced by a number of factors such as non-starch polysaccharides as well as some non-digestible oligo- and disaccharides. These molecules are only decomposed by intestinal anaerobic microbial fermentation, resulting in the formation of volatile fatty acids (VFAs), which play a central role in maintaining the balance of the intestinal flora and affecting the health status of the host organism. In the present study, the effects of lactulose and psyllium husk (Plantago ovata) were investigated regarding their influence on concentrations of various VFAs produced by the canine intestinal microbiome. Thirty dogs were kept on a standard diet for 15 days, during which time half of the animals received oral lactulose once a day, while the other group was given a psyllium-supplemented diet (in 0.67 and in 0.2 g/kg body weight concentrations, respectively). On days 0, 5, 10 and 15 of the experiment, feces were sampled from the rectum, and the concentration of each VFA was determined by GC-MS (gas chromatography−mass spectrometry). Lactulose administration caused a significant increase in the total VFA concentration of the feces on days 10 and 15 of the experiment (p = 0.035 and p < 0.001, respectively); however, in the case of psyllium supplementation, the concentration of VFAs showed a significant elevation only on day 15 (p = 0.003). Concentrations of acetate and propionate increased significantly on days 5, 10 and 15 after lactulose treatment (p = 0.044, p = 0.048 and p < 0.001, respectively). Following psyllium administration, intestinal acetate, propionate and n-butyrate production were stimulated on day 15, as indicated by the fecal VFA levels (p = 0.002, p = 0.035 and p = 0.02, respectively). It can be concluded that both lactulose and psyllium are suitable for enhancing the synthesis of VFAs in the intestines of dogs. Increased acetate and propionate concentrations were observed following the administration of both supplements; however, elevated n-butyrate production was found only after psyllium treatment, suggesting that the applied prebiotics may exert slightly different effects in the hindgut of dogs. These findings can be also of great importance regarding the treatment and management of patients suffering from intestinal disorders as well as hepatic encephalopathy due to portosystemic shunt.
Language	English
Publishing date	2022-04-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2768971-2
ISSN	2306-7381 ; 2306-7381
ISSN (online)	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci9050206
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Article ; Online: Serum hepcidin measurements in healthy dogs using liquid chromatography/tandem mass spectrometry.

Vizi, Zsuzsanna / Lányi, Katalin / Bagi, Melinda / Laczay, Péter / Balogh, Nándor / Sterczer, Ágnes

Veterinary clinical pathology

2020 Volume 49, Issue 2, Page(s) 292–298

Abstract: Background: Hepcidin is a key regulator of iron homeostasis. The measurement of this hormone is essential for the diagnosis of iron deficiency anemia and might be useful as a prognostic factor in many diseases. Serum hepcidin levels have been ... ...

Abstract	Background: Hepcidin is a key regulator of iron homeostasis. The measurement of this hormone is essential for the diagnosis of iron deficiency anemia and might be useful as a prognostic factor in many diseases. Serum hepcidin levels have been infrequently evaluated in dogs. Objectives: The aim of this study was to measure serum hepcidin in a population of healthy dogs using liquid chromatography/tandem mass spectrometry (LC-MS/MS), considered the gold standard assay for measuring hepcidin like in human medicine. Methods: Blood samples from 86 healthy dogs of 25 different breeds were measured with the LC-MS/MS method. Synthetic canine hepcidin was used as the standard reagent. Reference values were calculated based on the results. Results: The mean hepcidin concentration of the study population was 16.6 ± 7.7 ng/mL. There reference interval (RI) was defined as 5.3-36.4 ng/mL. No significant difference was found between male and female dogs, or between different age and body weight groups. Hepcidin concentrations did not correlate with red blood cell counts, hemoglobin concentrations, iron levels, iron-binding capacities, and C-reactive protein concentrations in this healthy population. A weak negative correlation was found between hepcidin and the mean corpuscular volume. Conclusion: LC-MS/MS proved to be a reliable and time-effective method for the detection of canine hepcidin. The RI was similar but narrower compared with that of human studies.
MeSH term(s)	Animals ; Chromatography, Liquid/veterinary ; Dogs/blood ; Female ; Hepcidins/blood ; Male ; Reference Values ; Tandem Mass Spectrometry/veterinary
Chemical Substances	Hepcidins
Language	English
Publishing date	2020-06-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2114702-4
ISSN	1939-165X ; 0275-6382
ISSN (online)	1939-165X
ISSN	0275-6382
DOI	10.1111/vcp.12872
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Article ; Online: Occurrence of Spirocerca lupi Infection in Hungarian Dogs Referred for Gastroscopy.

Psáder, Roland / Balogh, Márton / Pápa, Kinga / Sterczer, Ágnes / Lukács, Zoltán / Harnos, Andrea

Parasitology research

2017 Volume 116, Issue Suppl 1, Page(s) 99–108

Language	English
Publishing date	2017
Publishing country	Germany
Document type	Journal Article
ZDB-ID	284966-5
ISSN	1432-1955 ; 0932-0113 ; 0044-3255
ISSN (online)	1432-1955
ISSN	0932-0113 ; 0044-3255
DOI	10.1007/s00436-017-5496-9
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Article: Expression of claudins in the normal canine gastric mucosa.

Psáder, Roland / Jakab, Csaba / Máthé, Akos / Balka, Gyula / Pápa, Kinga / Sterczer, Agnes

Acta veterinaria Hungarica

2014 Volume 62, Issue 1, Page(s) 13–21

Abstract: The aim of the present study was to investigate the expression pattern of claudin-1, -2, -3, -4, -5, -7, -8, -10 and -18 in the intact fundic and pyloric gastric mucosa of dogs. Intense, linear, membranous claudin-18 positivity was detected in the ... ...

Abstract	The aim of the present study was to investigate the expression pattern of claudin-1, -2, -3, -4, -5, -7, -8, -10 and -18 in the intact fundic and pyloric gastric mucosa of dogs. Intense, linear, membranous claudin-18 positivity was detected in the surface gastric cells and in the epithelial cells of the gastric glands both in the fundic and pyloric stomach regions. The mucous neck cells in the apical part of the glands, furthermore the parietal cells and chief cells of the basal part of the gland were all positive for claudin-18, in the same way as the enteroendocrine cells. Cells of the basal part of the pyloric glands showed intense, linear, membranous claudin-2 positivity, but cells of the superficial portion of these glands and the surface gastric cells in this region were claudin-2 negative. Fibroblasts, endothelial cells, lymphocytes of the propria layer, smooth muscle cells and vegetative neurons were all negative for claudin-2. All gastric epithelial cells were negative for claudin-1, -3, -4, -5, -6, -7, -8 and -10. The endothelial cells of the propria layer had intense claudin-5 positivity. We assume that claudin-18 forms a paracellular barrier against gastric acid in the healthy canine stomach, in the same way as in mice.
Language	English
Publishing date	2014-03
Publishing country	Hungary
Document type	Journal Article
ZDB-ID	605742-1
ISSN	1588-2705 ; 0236-6290 ; 0365-8198
ISSN (online)	1588-2705
ISSN	0236-6290 ; 0365-8198
DOI	10.1556/AVet.2013.053
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Article: A case of synchronous hepatocellular carcinoma and aortic body chemodectoma in a dog - pathological case report.

Sterczer, Agnes / Németh, Tibor / Mándoki, Míra / Gálfi, Péter / Jakab, Csaba

Acta veterinaria Hungarica

2011 Volume 59, Issue 1, Page(s) 113–121

Abstract: The authors describe a case of synchronously occurring (double) tumours, i.e. primary hepatocellular carcinoma and aortic body chemodectoma in a 14-year-old mixed-breed male dog. The tumours were identified during necropsy, following euthanasia. In the ... ...

Abstract	The authors describe a case of synchronously occurring (double) tumours, i.e. primary hepatocellular carcinoma and aortic body chemodectoma in a 14-year-old mixed-breed male dog. The tumours were identified during necropsy, following euthanasia. In the last months of its life, the dog showed signs of weakness, anorexia, apathy, inactivity, and abdominal palpation elicited a painful reaction. The primary liver cancer emerged in the left lateral lobe without evidence of any distant metastases. Histopathological and immunohistochemical investigations revealed a well-differentiated, trabecular, claudin-7-, claudin-5- and pancytokeratin-negative hepatocellular carcinoma. The Ki-67 proliferation index was 33%. During necropsy, a synchronously occurring benign, grade I type aortic body chemodectoma was also detected in the dog. This neuroendocrine tumour showed chromogranin-, synaptophysin-, neuron-specific enolase- and S100 protein-positivity, and the Ki-67 proliferation index was 2%. The authors believe that this is the first description of synchronously occurring hepatocellular carcinoma and aortic body chemodectoma in a dog.
MeSH term(s)	Animals ; Aortic Bodies/pathology ; Carcinoma, Hepatocellular/pathology ; Carcinoma, Hepatocellular/veterinary ; Dog Diseases/pathology ; Dogs ; Liver Neoplasms/pathology ; Liver Neoplasms/veterinary ; Male ; Paraganglioma, Extra-Adrenal/pathology ; Paraganglioma, Extra-Adrenal/veterinary
Language	English
Publishing date	2011-03
Publishing country	Hungary
Document type	Case Reports ; Journal Article
ZDB-ID	605742-1
ISSN	1588-2705 ; 0236-6290 ; 0365-8198
ISSN (online)	1588-2705
ISSN	0236-6290 ; 0365-8198
DOI	10.1556/AVet.59.2011.1.10
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Article: Preparation of purified perikaryal and synaptosomal mitochondrial fractions from relatively small hypothalamic brain samples.

Kiss, David S / Toth, Istvan / Jocsak, Gergely / Sterczer, Agnes / Bartha, Tibor / Frenyo, Laszlo V / Zsarnovszky, Attila

MethodsX

2016 Volume 3, Page(s) 417–429

Abstract: In order to measure the activity of neuronal mitochondria, a representative proof of neuronal processes, physiologically relevant mitochondrial samples need to be gained as simply as possible. Existing methods are, however, either for tissue samples of ... ...

Abstract	In order to measure the activity of neuronal mitochondria, a representative proof of neuronal processes, physiologically relevant mitochondrial samples need to be gained as simply as possible. Existing methods are, however, either for tissue samples of large size and/or homogenous microstructures only, or are not tested for mitochondrial function measurements. In the present article we describe a gradient fractionation method to isolate viable and well-coupled mitochondria from relatively heterogeneous histological microstructures such as the hypothalamus. With this new method, we are able to isolate a sufficient amount of functional mitochondria for determination of respiratory activity, in a short period of time, using affordable equipment. •Verified by electron microscopy, our method separates highly enriched and well-preserved perikaryal and synaptosomal mitochondria. Both fractions contain minimal cell debris and no myelin. Respiratory measurements (carried out by Clark-type electrode) confirmed undisturbed mitochondrial function providing well-evaluable records. The demonstrated protocol yields highly viable mitochondrial subfractions within 3 h from small brain areas for high-precision examinations. Using this procedure, brain regions with relatively heterogeneous histological microstructure (hypothalamus) can also be efficiently sampled.•Up to our present knowledge, our method is the shortest available procedure with the lowest sample size to gain debris-free, fully-viable mitochondria.
Language	English
Publishing date	2016-05-19
Publishing country	Netherlands
Document type	Journal Article
ISSN	2215-0161
ISSN	2215-0161
DOI	10.1016/j.mex.2016.05.004
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Article: Effect of enteral feeding on gallbladder function in dogs.

Psáder, Roland / Sterczer, Agnes / Pápa, Kinga / Harnos, Andrea / Szilvási, Viktória / Pap, Akos

Acta veterinaria Hungarica

2012 Volume 60, Issue 2, Page(s) 211–222

Abstract: Nutritional support in critically ill patients is a fundamental principle of patient care. Little is known about gallbladder motility during the interdigestive phase and in response to enteral feeding. The objective of this study was to investigate the ... ...

Abstract	Nutritional support in critically ill patients is a fundamental principle of patient care. Little is known about gallbladder motility during the interdigestive phase and in response to enteral feeding. The objective of this study was to investigate the effect of enteral feeding on gallbladder function in dogs. The cholagogue meal (Lipofundin infusion) was applied in four anatomical positions (jejunum, duodenojejunal junction, descending duodenum, stomach) in five healthy Beagle dogs. Gallbladder volume (GBV) was monitored by ultrasonography. Lipofundin infusion given through the feeding tube caused a maximal gallbladder contraction of 9.2% (range 3.7-13.9%) in the jejunum, 16.5% (9.1-22.1%) at the duodenojejunal junction and 26.3% (22.8-29.5%) in the descending duodenum. When the cholagogue meal was given through the mouth, it caused a mean 33.8% (28.6-46.5%) maximum gallbladder contraction in the same animals. In conclusion, we can establish that the ultrasound-guided gallbladder emptying method proved to be a useful technique for monitoring the cholagogue effect of Lipofundin meal applied in different anatomical positions of the intestine. The deeper the position of application, the smaller and shorter gallbladder contraction was evoked.
MeSH term(s)	Animals ; Dogs ; Duodenum ; Enteral Nutrition ; Gallbladder ; Muscle Contraction
Language	English
Publishing date	2012-06
Publishing country	Hungary
Document type	Journal Article
ZDB-ID	605742-1
ISSN	1588-2705 ; 0236-6290 ; 0365-8198
ISSN (online)	1588-2705
ISSN	0236-6290 ; 0365-8198
DOI	10.1556/AVet.2012.018
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Article: Cholagogue-induced gallbladder emptying in the diagnosis of canine biliary obstruction.

Sterczer, Agnes / Reiczigel, Jenő / Psáder, Roland / Pápa, Kinga / Vörös, Károly / Pap, Akos

Acta veterinaria Hungarica

2012 Volume 60, Issue 2, Page(s) 199–209

Abstract: The efficacy of ultrasound-guided cholagogue-induced gallbladder emptying for differentiating obstructive from non-obstructive hepatobiliary diseases was studied in icteric dogs. In 7 healthy Beagle dogs, Lipofundin 20% infusion (2 ml/kg orally) evoked a ...

Abstract	The efficacy of ultrasound-guided cholagogue-induced gallbladder emptying for differentiating obstructive from non-obstructive hepatobiliary diseases was studied in icteric dogs. In 7 healthy Beagle dogs, Lipofundin 20% infusion (2 ml/kg orally) evoked a vigorous gallbladder contraction of 44.2% (range: 35.3-57.6%) and proved to be a useful, well-tolerable meal for routine use. In 24 icteric dogs, gallbladder contraction was evoked by different cholagogues: the maximum reduction in gallbladder volume (%) for the three non-obstructive icteric dogs was 43.9% (range, 39.0-46.5%). The average gallbladder contraction of the 21 dogs with biliary obstruction was less than 5%. In conclusion, the stimulation of gallbladder contraction with orally applied magnesium sulphate (MgSO4) or Lipofundin can be well demonstrated by ultrasound in dogs. The examination of cholagogue-induced gallbladder emptying is a valuable technique in icteric patients to indicate surgical intervention.
MeSH term(s)	Animals ; Cholagogues and Choleretics ; Cholestasis ; Dogs ; Gallbladder ; Gallbladder Emptying
Chemical Substances	Cholagogues and Choleretics
Language	English
Publishing date	2012-06
Publishing country	Hungary
Document type	Journal Article
ZDB-ID	605742-1
ISSN	1588-2705 ; 0236-6290 ; 0365-8198
ISSN (online)	1588-2705
ISSN	0236-6290 ; 0365-8198
DOI	10.1556/AVet.2012.017
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