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Article: Radish seed oil: Ultrasound-assisted extraction using ethanol as solvent and assessment of its potential for ester production

Stevanato, Natália / Camila da Silva

Industrial crops and products. 2019 June, v. 132

2019

Abstract: In this study, the ultrasound-assisted extraction (UAE) of radish seed oil (RSO) using ethanol as the solvent was investigated with the aim of ester synthesis by in situ reaction. An experimental design was used to evaluate the effects of temperature, ... ...

Abstract	In this study, the ultrasound-assisted extraction (UAE) of radish seed oil (RSO) using ethanol as the solvent was investigated with the aim of ester synthesis by in situ reaction. An experimental design was used to evaluate the effects of temperature, solvent to seed ratio and time on the efficiency of the UAE method and to enable the determination of the conditions that maximize the oil removal. The results were compared to those obtained with the classical extraction method, using ethanol and n-hexane as solvents, in terms of yield and oil characterization. The in situ reaction using the oil obtained by UAE was conducted applying different reaction times and enzyme loadings. The effect of using ultrasonic irradiation in the oil extraction and the in situ reaction was evaluated. The results showed that, under the conditions evaluated, the temperature had the greatest influence on the oil extraction, the maximum yield (˜25%) being obtained at 60 °C, with a solvent to seed ratio of 12 mL g−1 and extraction time of 60 min. This corresponds to ˜89% of the yield obtained by the classical method using n-hexane. The RSO was predominantly composed of oleic and erucic acids. The UAE allowed the extraction of a greater amount of phytosterols and tocopherols and, consequently, this oil presented greater oxidative stability. In addition, it was observed that the greater availability of the catalyst in the reaction medium favored the conversion of the oil to esters. The application of ultrasonic irradiation provided higher yields of the oil and ethyl esters.
Keywords	catalysts ; erucic acid ; esters ; ethanol ; experimental design ; hexane ; oleic acid ; oxidative stability ; phytosterols ; radishes ; seed oils ; solvents ; temperature ; ultrasonic treatment
Language	English
Dates of publication	2019-06
Size	p. 283-291.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1132158-1
ISSN	1872-633X ; 0926-6690
ISSN (online)	1872-633X
ISSN	0926-6690
DOI	10.1016/j.indcrop.2019.02.032
Database	NAL-Catalogue (AGRICOLA)

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Article: Simultaneous Extraction of Bioactive Compounds from

Jaski, Jonas Marcelo / da Cruz, Rayane Monique Sete / Pimentel, Tatiana Colombo / Stevanato, Natalia / da Silva, Camila / Barão, Carlos Eduardo / Cardozo-Filho, Lucio

Foods (Basel, Switzerland)

2023 Volume 12, Issue 5

Abstract: Olive leaves (OL) are products of olive cultivation with a high commercial value because they contain valuable bioactive compounds. Chia and sesame seeds have a high functional value because of their attractive nutritional properties. When combined in ... ...

Abstract	Olive leaves (OL) are products of olive cultivation with a high commercial value because they contain valuable bioactive compounds. Chia and sesame seeds have a high functional value because of their attractive nutritional properties. When combined in the extraction process, the two products constitute a product of high quality. The use of pressurized propane in vegetable oil extraction is advantageous because it provides solvent-free oil. This study aimed to combine two high-quality products to obtain oils with a unique combination of attractive nutritional properties and high levels of bioactive compounds. The mass percentage yields of the OL extracts with chia and sesame oils were 23.4% and 24.8%, respectively. The fatty acid profiles of the pure oils and their respective OL-enriched oils were similar. There was an aggregation of the 35% and 32% (
Language	English
Publishing date	2023-02-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12050948
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Article ; Online: Simultaneous Extraction of Bioactive Compounds from Olea europaea L. Leaves and Healthy Seed Oils Using Pressurized Propane

Jaski, Jonas Marcelo / da Cruz, Rayane Monique Sete / Pimentel, Tatiana Colombo / Stevanato, Natalia / da Silva, Camila / Barão, Carlos Eduardo / Cardozo-Filho, Lucio

Foods. 2023 Feb. 23, v. 12, no. 5

2023

Abstract	Olive leaves (OL) are products of olive cultivation with a high commercial value because they contain valuable bioactive compounds. Chia and sesame seeds have a high functional value because of their attractive nutritional properties. When combined in the extraction process, the two products constitute a product of high quality. The use of pressurized propane in vegetable oil extraction is advantageous because it provides solvent-free oil. This study aimed to combine two high-quality products to obtain oils with a unique combination of attractive nutritional properties and high levels of bioactive compounds. The mass percentage yields of the OL extracts with chia and sesame oils were 23.4% and 24.8%, respectively. The fatty acid profiles of the pure oils and their respective OL-enriched oils were similar. There was an aggregation of the 35% and 32% (v/v) bioactive OL compounds in chia and sesame oils, respectively. OL oils exhibited superior antioxidant capacities. The induction times of the OL extracts with the sesame and chia oils increased by 73% and 4.4%, respectively. Incorporating OL active compounds in healthy edible vegetable oils using propane as a solvent promotes the reduction of lipid oxidation, improves the lipid profiles and health indices of the oils, and forms a product with attractive nutritional characteristics.
Keywords	Olea europaea ; Salvia hispanica ; antioxidants ; fatty acids ; lipid peroxidation ; olives ; propane ; solvents ; vegetable oil ; vegetables
Language	English
Dates of publication	2023-0223
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12050948
Database	NAL-Catalogue (AGRICOLA)

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Article: Babassu kernel oil: Enhanced extraction and chemical characterization

Rasmussen, Pamela Pinheiro / Stevanato, Natália / Raspe, Djéssica Tatiane / Garcia, Vitor Augusto dos Santos / da Silva, Camila

Journal of food processing and preservation. 2022 June, v. 46, no. 6

2022

Abstract: The ultrasound‐assisted extraction (UAE) was applied to enhance the extraction efficiency of oil from babassu kernel (BKO) using n‐hexane as solvent. For this purpose, the effects of temperature (30 to 60°C), solvent to kernel ratio (6 to 10 ml g⁻¹), and ...

Abstract	The ultrasound‐assisted extraction (UAE) was applied to enhance the extraction efficiency of oil from babassu kernel (BKO) using n‐hexane as solvent. For this purpose, the effects of temperature (30 to 60°C), solvent to kernel ratio (6 to 10 ml g⁻¹), and time (10 to 30 min) on oil yield (Yₒ) were determined, in addition to assessing the effect of these variables in the characteristics of the obtained oil. In the condition of maximum extraction, the chemical composition of the oil was determined and the results were compared with the Soxhlet extraction. In the evaluated experimental range, the amount of solvent used in the process influenced the Yₒ, while time and temperature had no effect on the response variable. With this, a high oil removal of kernels (89.5% obtained by Soxhlet) was obtained in low conditions of time (10 min) and temperature (30°C), in addition to using a smaller amount of solvent (10 ml g⁻¹) in relation to conventional technique (30 ml g⁻¹). The lowest evaluated temperature (30°C) was also indicated to obtain an oil with higher content of phenolic compounds and antioxidant activity. BKO showed a predominance of lauric (~49.9%) and myristic acids (~15.6%), and high oxidative stability. The quality of the oil extracted by the UAE was superior in terms of phytosterols, phenolic compounds, antioxidant activity, and color parameters. PRACTICAL APPLICATIONS: Considered an emerging technique, ultrasound‐assisted extraction presents simplicity and the possibility of operational automation, lower energy and solvent consumption, in addition to the reduced complexity of the steps subsequent to extraction, composing a viable and suitable alternative for a possible industrial application. The oil obtained by the aforementioned technique resulted from mild operating conditions, presenting quality parameters that stand out and contemplate promising technological criteria in the global market demand for compounds from natural sources.
Keywords	antioxidant activity ; automation ; chemical composition ; color ; energy ; hexane ; industrial applications ; oxidative stability ; phytosterols ; seed oils ; seeds ; solid phase extraction ; solvents ; supply balance ; temperature ; ultrasonic treatment ; world markets
Language	English
Dates of publication	2022-06
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	243448-9
ISSN	1745-4549 ; 0145-8892
ISSN (online)	1745-4549
ISSN	0145-8892
DOI	10.1111/jfpp.16559
Database	NAL-Catalogue (AGRICOLA)

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Article: Simultaneous extraction of sunflower oil and active compounds from olive leaves using pressurized propane

Jaski, Jonas Marcelo / Abrantes, Karen Keli Barbosa / Zanqui, Ana Beatriz / Stevanato, Natalia / da Silva, Camila / Barão, Carlos Eduardo / Bonfim-Rocha, Lucas / Cardozo-Filho, Lúcio

Current research in food science. 2022, v. 5

2022

Abstract: Sunflower is grown in different parts of the world and oil from the grain has many uses, including cosmetics and food. Olive leaves are rich in active compounds with potential for industrial use. The simultaneous extraction of raw materials is an ... ...

Abstract	Sunflower is grown in different parts of the world and oil from the grain has many uses, including cosmetics and food. Olive leaves are rich in active compounds with potential for industrial use. The simultaneous extraction of raw materials is an economical and sustainable way of using the same extraction process to obtain products with high added value. The aim of this work was to promote the incorporation of bioactive compounds from olive leaves in sunflower oil by two extraction techniques: pressurized propane (PRO) and Soxhlet (SOX) and to evaluate the increase in oxidative stability and antioxidant activity of oils. The techniques used were useful in producing sunflower oil incorporating olive leaf extract (SFO + OLE); 4.3% 1-octacosanol and 5.8% 1-triacontanol were incorporated, and β-sitosterol increased by at least 90%. Also, SFO + OLE showed an increase in the induction time of 2.7 and 3.7 h compared to SFO for the PRO and SOX methods, respectively. The profile of fatty acids was maintained, with the majority in all samples being oleic and linoleic acids. Consequently, with this procedure is possible to produce SFO + OLE with better antioxidant activity and better nutritional characteristics using PRO and SOX. The scaled-up of the simultaneous extraction process via pressurized propane is economically viable according to the process simulation and economic evaluation.
Keywords	Helianthus annuus ; antioxidant activity ; cosmetics ; economic evaluation ; economic sustainability ; leaf extracts ; olives ; oxidative stability ; propane ; research ; sunflower oil
Language	English
Size	p. 531-544.
Publishing place	Elsevier B.V.
Document type	Article
ISSN	2665-9271
DOI	10.1016/j.crfs.2022.03.002
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Production of HPMC-films for lactase administration.

Silva, HeloÍsa R P DA / Stevanato, NatÁlia / Garcia, Vitor A S / Silva, Camila DA

Anais da Academia Brasileira de Ciencias

2020 Volume 92, Issue 4, Page(s) e20200348

Abstract: The present work aimed to develop and characterize HPMC-films (HFs) based on hydroxypropylmethylcellulose (HPMC) with the addition of lactase for later application in the reduction of lactose in whole milk. The HFs were produced by the casting technique, ...

Abstract	The present work aimed to develop and characterize HPMC-films (HFs) based on hydroxypropylmethylcellulose (HPMC) with the addition of lactase for later application in the reduction of lactose in whole milk. The HFs were produced by the casting technique, with the addition of 1, 3, 5 and 7 g of lactase per 100 g of filmogenic solution. All the formulations presented a high capacity of film formation, but the thickness and time of disintegration increased according to the added lactase concentration. Regardless of the formulation developed, HFs in whole milk, a reduction in the lactose content (~ 80%) occurred, classifying the product as zero lactose. Thus, the HPMC-films with the addition of lactase can be considered as a good alternative for solubilization in foods which have a high concentration of lactose.
MeSH term(s)	Animals ; Hypromellose Derivatives ; Lactase ; Lactose ; Milk
Chemical Substances	Hypromellose Derivatives (3NXW29V3WO) ; Lactase (EC 3.2.1.108) ; Lactose (J2B2A4N98G)
Language	English
Publishing date	2020-12-14
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2046885-4
ISSN	1678-2690 ; 0001-3765
ISSN (online)	1678-2690
ISSN	0001-3765
DOI	10.1590/0001-3765202020200348
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Pumpkin (Cucurbita maxima) by‐products: Obtaining seed oil enriched with active compounds from the peel by ultrasonic‐assisted extraction

Massa, Thainara Bovo / Stevanato, Natália / Cardozo‐Filho, Lúcio / da Silva, Camila

Journal of food process engineering. 2019 Aug., v. 42, no. 5

2019

Abstract: Ultrasonic‐assisted extraction (UAE) of the oil from the seeds/peel mixture of pumpkin using ethanol as solvent was investigated. The effects of the ultrasound, solvent volume, time, and temperature on the oil yield were evaluated. Oil extraction from ... ...

Abstract	Ultrasonic‐assisted extraction (UAE) of the oil from the seeds/peel mixture of pumpkin using ethanol as solvent was investigated. The effects of the ultrasound, solvent volume, time, and temperature on the oil yield were evaluated. Oil extraction from the seeds by ultrasound and the mixture by Soxhlet extraction (SE) was also performed. The use of ultrasound and higher availability of ethanol (up to 6 mL g⁻¹ sample) favored the oil removal. In the extraction kinetics, the washing step was verified in the first 20 min, corresponding to 79–89% of the total oil yield. The diffusion coefficient increased from 30 to 75°C, as well as the spontaneity of the extraction, achieving 71% of the SE. Linoleic and oleic acids were found in higher concentrations in the oils. UAE made possible the greater extraction of β‐carotene, tocopherols, and fistosterois, and the peel addition provided higher contents of these compounds. PRACTICAL APPLICATION: The simultaneous extraction of compounds from the pumpkin by‐products arouses interest, for obtaining an oil of the seeds enriched with the active compounds present in the peel. Ultrasound‐assisted extraction was employed because it showed relevant benefits in relation to Soxhlet extraction, such as operations with reduced time, mild temperatures, and low amounts of solvent. Aiming at the use of solvents considered as “green,” in the present study we opted for the use of ethanol, since it is considered a safe solvent and comes from renewable sources. In addition, the determination of the diffusion coefficient and the thermodynamic properties of the process helped to understand the mechanisms that characterize the extraction.
Keywords	Cucurbita maxima ; active ingredients ; beta-carotene ; byproducts ; diffusivity ; ethanol ; linoleic acid ; oleic acid ; pumpkins ; seed oils ; seeds ; solid phase extraction ; solvents ; temperature ; thermodynamics ; tocopherols ; ultrasonic treatment ; ultrasonics ; washing
Language	English
Dates of publication	2019-08
Publishing place	John Wiley & Sons, Inc.
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	752358-0
ISSN	0145-8876
ISSN	0145-8876
DOI	10.1111/jfpe.13125
Database	NAL-Catalogue (AGRICOLA)

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Article: Sunflower oil from enzymatic aqueous extraction process: Maximization of free oil yield and oil characterization

de Aquino, Denise Silva / Fanhani, Anastassia / Stevanato, Natália / da Silva, Camila

Journal of food process engineering. 2019 Oct., v. 42, no. 6

2019

Abstract: In this article, the enzymatic aqueous extraction (EAE) of sunflower seed oil was investigated. The effects of the operational variables (temperature, water seed mass ratio, and enzyme concentration) were evaluated in order to define the conditions that ... ...

Abstract	In this article, the enzymatic aqueous extraction (EAE) of sunflower seed oil was investigated. The effects of the operational variables (temperature, water seed mass ratio, and enzyme concentration) were evaluated in order to define the conditions that maximize the free oil yield (FOY). It was observed that increasing the amounts of water and enzyme in the extraction medium was disadvantageous to the removal of the oil, and that an increase in temperature provided a higher FOY. Thus, a temperature of 60°C, seed:water ratio of 1:5 (g/g), and enzyme concentration of 1% (v/v) were defined as the conditions for maximum FOY (17.76%). The use of a buffered medium under these conditions increased the FOY to 20.34%. The composition of the oils obtained by the EAE under different experimental conditions was determined and compared with the oil obtained from Soxhlet extraction. The oils under study presented high levels of oleic and linoleic acids, which corresponded to ~90% of the composition of fatty acids. It was also noted that, depending on the extraction conditions, the oils obtained by EAE presented phytosterol and tocopherol contents similar or superior to those of the oil produced by Soxhlet extraction. PRACTICAL APPLICATIONS: The enzymatic aqueous extraction of sunflower oil has the potential to replace the solvent extraction method. The results reported herein show that the composition of the sunflower oil obtained by the enzymatic method was similar to that of the oil extracted by the solvent method. In addition, the enzymatic method uses mild conditions that reduce the energy required for the process and the residues are free of organic solvent.
Keywords	energy ; enzymatic treatment ; fatty acid composition ; linoleic acid ; oleic acid ; phytosterols ; seed weight ; solid phase extraction ; solvents ; sunflower oil ; temperature
Language	English
Dates of publication	2019-10
Publishing place	John Wiley & Sons, Inc.
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	752358-0
ISSN	0145-8876
ISSN	0145-8876
DOI	10.1111/jfpe.13169
Database	NAL-Catalogue (AGRICOLA)

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Article: Obtaining fermentable sugars and fiber concentrate from asparagus by‐product

de Paula Laidens, Carolina / Iwassa, Isabela Julio / Stevanato, Natália / Zampar, Isabella Carolina / Bolanho Barros, Beatriz Cervejeira / da Silva, Camila

Journal of food processing and preservation. 2021 July, v. 45, no. 7

2021

Abstract: In this study, different solid–liquid extraction conditions to obtain fermentable sugars from an asparagus by‐product were investigated to produce a fiber concentrate. To evaluate the effects of agitation, solvent:sample ratio and temperature, an ... ...

Abstract	In this study, different solid–liquid extraction conditions to obtain fermentable sugars from an asparagus by‐product were investigated to produce a fiber concentrate. To evaluate the effects of agitation, solvent:sample ratio and temperature, an experimental design was used and the maximum extraction conditions of reducing sugars (RS) were determined. The diffusion coefficient of sugars in water at different temperatures was obtained, as well as the thermodynamic parameters of the process. The materials obtained before and after the extraction were characterized as composition, technological properties, and functional groups. The results showed that, under the conditions evaluated, agitation had the highest influence on the RS extraction, followed by the solvent:sample ratio and temperature. The maximum RS content (~30 g 100 g⁻¹ dry asparagus by‐product) was obtained at 125 rpm, 60°C, 30 min, and solvent:sample ratio of 20 ml/g. The values for the diffusion coefficient were directly proportional to the temperature increase, and the extraction was characterized as endothermic, irreversible, and spontaneous. The infrared spectrum showed that RS extraction did not alter the functional groups present in the sample, but this treatment promoted the concentration of soluble and insoluble dietary fiber, improving the balance of these fractions and the technological properties. PRACTICAL APPLICATIONS: The asparagus by‐product used in this study is of interest because it has a high dietary fiber content. Maximizing the reducing sugars extraction is necessary since the high amount of these compounds makes it difficult to employ the material in the development of new functional products. In addition, the extract rich in fermentable sugars obtained has potential use in the alcoholic fermentation. Therefore, the use of eco‐friendly solubilization techniques is of interest to improve the composition and properties of the fiber concentrate obtained after this process. In addition, the determination of the thermodynamic parameters of the process and the diffusion coefficient allowed a better understanding of the mechanisms that characterize the extraction.
Keywords	agitation ; alcoholic fermentation ; byproducts ; dietary fiber ; diffusivity ; endothermy ; experimental design ; fiber content ; solubilization ; temperature ; thermodynamics
Language	English
Dates of publication	2021-07
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	243448-9
ISSN	1745-4549 ; 0145-8892
ISSN (online)	1745-4549
ISSN	0145-8892
DOI	10.1111/jfpp.15640
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Simultaneous extraction of sunflower oil and active compounds from olive leaves using pressurized propane.

Jaski, Jonas Marcelo / Abrantes, Karen Keli Barbosa / Zanqui, Ana Beatriz / Stevanato, Natalia / da Silva, Camila / Barão, Carlos Eduardo / Bonfim-Rocha, Lucas / Cardozo-Filho, Lúcio

Current research in food science

2022 Volume 5, Page(s) 531–544

Abstract	Sunflower is grown in different parts of the world and oil from the grain has many uses, including cosmetics and food. Olive leaves are rich in active compounds with potential for industrial use. The simultaneous extraction of raw materials is an economical and sustainable way of using the same extraction process to obtain products with high added value. The aim of this work was to promote the incorporation of bioactive compounds from olive leaves in sunflower oil by two extraction techniques: pressurized propane (PRO) and Soxhlet (SOX) and to evaluate the increase in oxidative stability and antioxidant activity of oils. The techniques used were useful in producing sunflower oil incorporating olive leaf extract (SFO + OLE); 4.3% 1-octacosanol and 5.8% 1-triacontanol were incorporated, and β-sitosterol increased by at least 90%. Also, SFO + OLE showed an increase in the induction time of 2.7 and 3.7 h compared to SFO for the PRO and SOX methods, respectively. The profile of fatty acids was maintained, with the majority in all samples being oleic and linoleic acids. Consequently, with this procedure is possible to produce SFO + OLE with better antioxidant activity and better nutritional characteristics using PRO and SOX. The scaled-up of the simultaneous extraction process via pressurized propane is economically viable according to the process simulation and economic evaluation.
Language	English
Publishing date	2022-03-10
Publishing country	Netherlands
Document type	Journal Article
ISSN	2665-9271
ISSN (online)	2665-9271
DOI	10.1016/j.crfs.2022.03.002
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