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  1. Article ; Online: Video feedback and e-Learning enhances laboratory skills and engagement in medical laboratory science students.

    Donkin, Rebecca / Askew, Elizabeth / Stevenson, Hollie

    BMC medical education

    2019  Volume 19, Issue 1, Page(s) 310

    Abstract: Background: Traditionally, the training of medical laboratory science students has taken place in the laboratory and has been led by academic and pathology experts in a face-to-face context. In recent years, budgetary pressures, increasing student ... ...

    Abstract Background: Traditionally, the training of medical laboratory science students has taken place in the laboratory and has been led by academic and pathology experts in a face-to-face context. In recent years, budgetary pressures, increasing student enrolments and limited access to laboratory equipment have resulted in reduced staff-student contact hours in medical laboratory science education. While this restructure in resources has been challenging, it has encouraged innovation in online blended learning.
    Methods: Blended learning histology lessons were implemented in a face-to-face and e-Learning format in a medical laboratory science program to teach tissue morphology and technical procedures outside of the traditional laboratory classroom. Participating students were randomly allocated to either the 'video' group (n = 14) or the 'control' group (n = 14). After all students attempted the e-Learning lessons and viewed expert-led video recordings online, students demonstrated their hands-on practical skills in the laboratory. Technical skills, demonstration of safety awareness, and use of histology equipment was captured by video through first person 'point of view' recordings for the 'video' group only. The 'control' group performed the same activities but were not recorded. Prior to summative assessment, the 'video' group students had a digital resource portfolio that enabled them to review their skills, receive captured feedback and retain a visual copy of their recorded procedure.
    Results: Results showed that students who participated in the online video format had statistically better practical examination scores and final grades compared to the control group.
    Conclusion: Findings from this study suggest that students are engaged and motivated when being taught in a blended learning format and respond positively to the use of video recordings with expert feedback for the initial learning of hands-on techniques. For the academic, developing a blended learning medical laboratory science program, which includes annotated virtual microscopy, video demonstrations, and online interactive e-Learning activities, provides an effective and economic approach to learning and teaching.
    MeSH term(s) Clinical Clerkship ; Clinical Competence ; Competency-Based Education/statistics & numerical data ; Computer-Assisted Instruction/statistics & numerical data ; Education, Distance/statistics & numerical data ; Feedback ; Humans ; Medical Laboratory Science/education ; Motivation ; Program Evaluation ; Students, Medical ; Video Recording
    Language English
    Publishing date 2019-08-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2044473-4
    ISSN 1472-6920 ; 1472-6920
    ISSN (online) 1472-6920
    ISSN 1472-6920
    DOI 10.1186/s12909-019-1745-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Population Dynamics of Vibrio and Pseudomonas Species Isolated from Farmed Tasmanian Atlantic Salmon (Salmo salar L.): A Seasonal Study

    Hatje, Eva / Neuman, Christina / Stevenson, Hollie / Bowman, John P / Katouli, Mohammad

    Microbial ecology. 2014 Nov., v. 68, no. 4

    2014  

    Abstract: Vibrio and Pseudomonas species have been shown to be part of the normal microbiota of Atlantic salmon (Salmo salar L.), with some strains causing disease in fish. The factors affecting their prevalence and persistence in the salmon gut, however, have not ...

    Abstract Vibrio and Pseudomonas species have been shown to be part of the normal microbiota of Atlantic salmon (Salmo salar L.), with some strains causing disease in fish. The factors affecting their prevalence and persistence in the salmon gut, however, have not been well studied. In this study, we collected 340 Vibrio and 150 Pseudomonas isolates from the hindgut of farmed Tasmanian Atlantic salmon, fed with two commercially available diets. Samples were collected every 6–8 weeks between July 2011 and May 2012. Isolates from selective agar were initially identified using biochemical tests and confirmed using genus-specific primers and 16S ribosomal RNA (16S rRNA) sequencing. Random amplified polymorphic DNA (RAPD) PCR was used to type both Pseudomonas and Vibrio; the latter was further typed using a biochemical fingerprinting method (PhP-RV plates). We observed low species diversity with strains comprising Vibrio ichthyoenteri/Vibrio scophthalmi, Vibrio crassostreae/Vibrio splendidus, Aliivibrio finisterrensis, Photobacterium phosphoreum and Pseudomonas fragi. Out of 340 Vibrio isolates, 238 (70 %) belonged to 21 clonal types and were found predominantly during summer when water temperatures reached 15 to 21 °C. Of these, the four major clonal types were found in multiple samples (70 %). P. fragi, on the other hand, was only found during the colder water temperatures and belonged to 18 clonal types. The presence of both groups of bacteria and their clonal types were independent of the fish diets used, suggesting that the water temperature was the main factor of the prevalence and persistence of these bacteria in the gut of Atlantic salmon.
    Keywords DNA ; Photobacterium phosphoreum ; Pseudomonas fragi ; Salmo salar ; Vibrio ichthyoenteri ; Vibrio scophthalmi ; Vibrio splendidus ; agar ; bacteria ; diet ; hindgut ; intestinal microorganisms ; population dynamics ; random amplified polymorphic DNA technique ; ribosomal RNA ; salmon ; species diversity ; summer ; water temperature
    Language English
    Dates of publication 2014-11
    Size p. 679-687.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 1462065-0
    ISSN 1432-184X ; 0095-3628
    ISSN (online) 1432-184X
    ISSN 0095-3628
    DOI 10.1007/s00248-014-0462-x
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Population dynamics of Vibrio and Pseudomonas species isolated from farmed Tasmanian Atlantic salmon (Salmo salar L.): a seasonal study.

    Hatje, Eva / Neuman, Christina / Stevenson, Hollie / Bowman, John P / Katouli, Mohammad

    Microbial ecology

    2014  Volume 68, Issue 4, Page(s) 679–687

    Abstract: Vibrio and Pseudomonas species have been shown to be part of the normal microbiota of Atlantic salmon (Salmo salar L.), with some strains causing disease in fish. The factors affecting their prevalence and persistence in the salmon gut, however, have not ...

    Abstract Vibrio and Pseudomonas species have been shown to be part of the normal microbiota of Atlantic salmon (Salmo salar L.), with some strains causing disease in fish. The factors affecting their prevalence and persistence in the salmon gut, however, have not been well studied. In this study, we collected 340 Vibrio and 150 Pseudomonas isolates from the hindgut of farmed Tasmanian Atlantic salmon, fed with two commercially available diets. Samples were collected every 6-8 weeks between July 2011 and May 2012. Isolates from selective agar were initially identified using biochemical tests and confirmed using genus-specific primers and 16S ribosomal RNA (16S rRNA) sequencing. Random amplified polymorphic DNA (RAPD) PCR was used to type both Pseudomonas and Vibrio; the latter was further typed using a biochemical fingerprinting method (PhP-RV plates). We observed low species diversity with strains comprising Vibrio ichthyoenteri/Vibrio scophthalmi, Vibrio crassostreae/Vibrio splendidus, Aliivibrio finisterrensis, Photobacterium phosphoreum and Pseudomonas fragi. Out of 340 Vibrio isolates, 238 (70 %) belonged to 21 clonal types and were found predominantly during summer when water temperatures reached 15 to 21 °C. Of these, the four major clonal types were found in multiple samples (70 %). P. fragi, on the other hand, was only found during the colder water temperatures and belonged to 18 clonal types. The presence of both groups of bacteria and their clonal types were independent of the fish diets used, suggesting that the water temperature was the main factor of the prevalence and persistence of these bacteria in the gut of Atlantic salmon.
    MeSH term(s) Animals ; Aquaculture ; Fish Diseases/epidemiology ; Fish Diseases/microbiology ; Molecular Sequence Data ; Polymerase Chain Reaction/veterinary ; Population Dynamics ; Pseudomonas/classification ; Pseudomonas/isolation & purification ; Pseudomonas Infections/epidemiology ; Pseudomonas Infections/microbiology ; Pseudomonas Infections/veterinary ; RNA, Ribosomal, 16S/genetics ; Random Amplified Polymorphic DNA Technique/veterinary ; Salmo salar ; Seasons ; Sequence Analysis, DNA/veterinary ; Tasmania/epidemiology ; Vibrio/classification ; Vibrio/isolation & purification ; Vibrio Infections/epidemiology ; Vibrio Infections/microbiology ; Vibrio Infections/veterinary
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2014-07-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1462065-0
    ISSN 1432-184X ; 0095-3628
    ISSN (online) 1432-184X
    ISSN 0095-3628
    DOI 10.1007/s00248-014-0462-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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  4. Article: Population Dynamics of Vibrio and Pseudomonas Species Isolated from Farmed Tasmanian Atlantic Salmon (Salmo salar L.): A Seasonal Study

    Hatje, Eva / Neuman, Christina / Stevenson, Hollie / Bowman, John P. / Katouli, Mohammad

    Microbial ecology

    Volume v. 68,, Issue no. 4

    Abstract: Vibrio and Pseudomonas species have been shown to be part of the normal microbiota of Atlantic salmon (Salmo salar L.), with some strains causing disease in fish. The factors affecting their prevalence and persistence in the salmon gut, however, have not ...

    Abstract Vibrio and Pseudomonas species have been shown to be part of the normal microbiota of Atlantic salmon (Salmo salar L.), with some strains causing disease in fish. The factors affecting their prevalence and persistence in the salmon gut, however, have not been well studied. In this study, we collected 340 Vibrio and 150 Pseudomonas isolates from the hindgut of farmed Tasmanian Atlantic salmon, fed with two commercially available diets. Samples were collected every 6–8 weeks between July 2011 and May 2012. Isolates from selective agar were initially identified using biochemical tests and confirmed using genus-specific primers and 16S ribosomal RNA (16S rRNA) sequencing. Random amplified polymorphic DNA (RAPD) PCR was used to type both Pseudomonas and Vibrio; the latter was further typed using a biochemical fingerprinting method (PhP-RV plates). We observed low species diversity with strains comprising Vibrio ichthyoenteri/Vibrio scophthalmi, Vibrio crassostreae/Vibrio splendidus, Aliivibrio finisterrensis, Photobacterium phosphoreum and Pseudomonas fragi. Out of 340 Vibrio isolates, 238 (70 %) belonged to 21 clonal types and were found predominantly during summer when water temperatures reached 15 to 21 °C. Of these, the four major clonal types were found in multiple samples (70 %). P. fragi, on the other hand, was only found during the colder water temperatures and belonged to 18 clonal types. The presence of both groups of bacteria and their clonal types were independent of the fish diets used, suggesting that the water temperature was the main factor of the prevalence and persistence of these bacteria in the gut of Atlantic salmon.
    Keywords random amplified polymorphic DNA technique ; Photobacterium phosphoreum ; Vibrio splendidus ; diet ; agar ; ribosomal RNA ; Pseudomonas fragi ; Salmo salar ; Vibrio scophthalmi ; bacteria ; DNA ; summer ; salmon ; intestinal microorganisms ; hindgut ; water temperature ; Vibrio ichthyoenteri ; species diversity ; population dynamics
    Language English
    Document type Article
    ISSN 0095-3628
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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