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Article ; Online: Sodium and risk assessment of osmotic demyelination syndrome: the method matters!

Stove, Veronique V / Oyaert, Matthijs / Delanghe, Joris R

Clinical chemistry and laboratory medicine

2023 Volume 62, Issue 1, Page(s) e22–e23

MeSH term(s)	Humans ; Sodium ; Demyelinating Diseases/diagnosis ; Hyponatremia/diagnosis ; Risk Assessment
Chemical Substances	Sodium (9NEZ333N27)
Language	English
Publishing date	2023-07-27
Publishing country	Germany
Document type	Letter ; Comment
ZDB-ID	1418007-8
ISSN	1437-4331 ; 1434-6621 ; 1437-8523
ISSN (online)	1437-4331
ISSN	1434-6621 ; 1437-8523
DOI	10.1515/cclm-2023-0667
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Article ; Online: Pancreatic lipase assays: time for a change towards immunoassays?

Delanghe, Joris R / Oyaert, Matthijs N / Stove, Veronique V

Clinical chemistry and laboratory medicine

2022 Volume 60, Issue 3, Page(s) 75–76

MeSH term(s)	Humans ; Immunoassay ; Lipase
Chemical Substances	Lipase (EC 3.1.1.3)
Language	English
Publishing date	2022-01-07
Publishing country	Germany
Document type	Letter ; Comment
ZDB-ID	1418007-8
ISSN	1437-4331 ; 1434-6621 ; 1437-8523
ISSN (online)	1437-4331
ISSN	1434-6621 ; 1437-8523
DOI	10.1515/cclm-2021-1245
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Article ; Online: Quantitative urine test strip reading for leukocyte esterase and hemoglobin peroxidase.

Oyaert, Matthijs N / Himpe, Jonas / Speeckaert, Marijn M / Stove, Veronique V / Delanghe, Joris R

Clinical chemistry and laboratory medicine

2018 Volume 56, Issue 7, Page(s) 1126–1132

Abstract: Background: Recently, urine test strip readers have become available for automated test strip analysis. We explored the possibilities of the Sysmex UC-3500 automated urine chemistry analyzer based on complementary metal oxide semiconductor (CMOS) sensor ...

Abstract	Background: Recently, urine test strip readers have become available for automated test strip analysis. We explored the possibilities of the Sysmex UC-3500 automated urine chemistry analyzer based on complementary metal oxide semiconductor (CMOS) sensor technology with regard to accuracy of leukocyte esterase and hemoglobin peroxidase results. We studied the influence of possible confounders on these measurements. Methods: Reflectance data of leukocyte esterase and hemoglobin peroxidase were measured using CMOS technology on the Sysmex UC-3500 automated urine chemistry analyzer. Analytical performance (imprecision, LOQ) as well as the correlation with white blood cell (WBC) and red blood cell (RBC) counts (Sysmex UF-5000) were studied. Furthermore, the influence of urinary dilution, haptoglobin, pH and ascorbic acid as confounders was determined. Results: Within- and between-run imprecision (reflectance signal) ranged from 1.1% to 3.6% and 0.9% to 4.2% for peroxidase and 0.4% to 2.5% and 0.4% to 3.3% for leukocyte esterase. Good agreement was obtained between the UF-5000 for RBCs and peroxidase reflectance (r=0.843) and for WBCs and leukocyte esterase (r=0.821). Specific esterase activity decreased for WBC counts exceeding 100 cells/μL. Haptoglobin influenced the peroxidase activity, whereas leukocyte esterase and peroxidase activities showed a pH optimum between 5.0 and 6.5. A sigmoidal correlation was observed between urinary osmolality and peroxidase activity. Conclusions: CMOS technology allows to obtain high quality test strip results for assessing WBC and RBC in urine. Quantitative peroxidase and leukocyte esterase are complementary with flow cytometry and have an added value in urinalysis, which may form a basis for expert system development.
MeSH term(s)	Carboxylic Ester Hydrolases/chemistry ; Carboxylic Ester Hydrolases/urine ; Erythrocyte Count/methods ; Haptoglobins/chemistry ; Hemoglobins/chemistry ; Hemoglobinuria/urine ; Humans ; Hydrogen-Ion Concentration ; Leukocyte Count/methods ; Peroxidases/chemistry ; Peroxidases/urine ; Urinalysis/instrumentation ; Urinalysis/methods
Chemical Substances	Haptoglobins ; Hemoglobins ; Peroxidases (EC 1.11.1.-) ; leukocyte esterase (EC 3.1.-) ; Carboxylic Ester Hydrolases (EC 3.1.1.-)
Language	English
Publishing date	2018-02-09
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1418007-8
ISSN	1437-4331 ; 1434-6621 ; 1437-8523
ISSN (online)	1437-4331
ISSN	1434-6621 ; 1437-8523
DOI	10.1515/cclm-2017-1159
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Article ; Online: Prediction of the hematocrit of dried blood spots via potassium measurement on a routine clinical chemistry analyzer.

Capiau, Sara / Stove, Veronique V / Lambert, Willy E / Stove, Christophe P

Analytical chemistry

2013 Volume 85, Issue 1, Page(s) 404–410

Abstract: The potential of dried blood spot (DBS) sampling as an alternative for classical venous sampling is increasingly recognized, with multiple applications in, e.g., therapeutic drug monitoring and toxicology. Although DBS sampling has many advantages, it is ...

Abstract	The potential of dried blood spot (DBS) sampling as an alternative for classical venous sampling is increasingly recognized, with multiple applications in, e.g., therapeutic drug monitoring and toxicology. Although DBS sampling has many advantages, it is associated with several issues, the hematocrit (Hct) issue being the most widely discussed challenge, given its possible strong impact on DBS-based quantitation. Hitherto, no approaches allow Hct prediction from nonvolumetrically applied DBS. Following a simple and rapid extraction protocol, K(+) levels from 3 mm DBS punches were measured via indirect potentiometry, using the Roche Cobas 8000 routine chemistry analyzer. The extracts' K(+) concentrations were used to calculate the approximate Hct of the blood used to generate DBS. A linear calibration line was established, with a Hct range of 0.19 to 0.63 (lower limit of quantification, LLOQ, to upper limit of quantification, ULOQ). The procedure was fully validated; the bias and imprecision of quality controls (QCs) at three Hct levels and at the LLOQ and ULOQ was less than 5 and 12%, respectively. In addition, the influence of storage (pre- and postextraction), volume spotted, and punch homogeneity was evaluated. Application on DBS from patient samples (n = 111), followed by Bland and Altman, Passing and Bablok, and Deming regression analysis, demonstrated a good correlation between the "predicted Hct" and the "actual Hct". After correcting for the observed bias, limits of agreement of ±0.049 were established. Incurred sample reanalysis demonstrated assay reproducibility. In conclusion, potassium levels in extracts from 3 mm DBS punches can be used to get a good prediction of the Hct, one of the most important "unknowns" in DBS analysis.
MeSH term(s)	Blood Chemical Analysis ; Dried Blood Spot Testing ; Electrochemical Techniques ; Hematocrit ; Humans ; Potassium/blood ; Regression Analysis
Chemical Substances	Potassium (RWP5GA015D)
Language	English
Publishing date	2013-01-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/ac303014b
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Article ; Online: Potassium-based algorithm allows correction for the hematocrit bias in quantitative analysis of caffeine and its major metabolite in dried blood spots.

De Kesel, Pieter M M / Capiau, Sara / Stove, Veronique V / Lambert, Willy E / Stove, Christophe P

Analytical and bioanalytical chemistry

2014 Volume 406, Issue 26, Page(s) 6749–6755

Abstract: Although dried blood spot (DBS) sampling is increasingly receiving interest as a potential alternative to traditional blood sampling, the impact of hematocrit (Hct) on DBS results is limiting its final breakthrough in routine bioanalysis. To predict the ... ...

Abstract	Although dried blood spot (DBS) sampling is increasingly receiving interest as a potential alternative to traditional blood sampling, the impact of hematocrit (Hct) on DBS results is limiting its final breakthrough in routine bioanalysis. To predict the Hct of a given DBS, potassium (K(+)) proved to be a reliable marker. The aim of this study was to evaluate whether application of an algorithm, based upon predicted Hct or K(+) concentrations as such, allowed correction for the Hct bias. Using validated LC-MS/MS methods, caffeine, chosen as a model compound, was determined in whole blood and corresponding DBS samples with a broad Hct range (0.18-0.47). A reference subset (n = 50) was used to generate an algorithm based on K(+) concentrations in DBS. Application of the developed algorithm on an independent test set (n = 50) alleviated the assay bias, especially at lower Hct values. Before correction, differences between DBS and whole blood concentrations ranged from -29.1 to 21.1%. The mean difference, as obtained by Bland-Altman comparison, was -6.6% (95% confidence interval (CI), -9.7 to -3.4%). After application of the algorithm, differences between corrected and whole blood concentrations lay between -19.9 and 13.9% with a mean difference of -2.1% (95% CI, -4.5 to 0.3%). The same algorithm was applied to a separate compound, paraxanthine, which was determined in 103 samples (Hct range, 0.17-0.47), yielding similar results. In conclusion, a K(+)-based algorithm allows correction for the Hct bias in the quantitative analysis of caffeine and its metabolite paraxanthine.
MeSH term(s)	Algorithms ; Caffeine/blood ; Dried Blood Spot Testing/methods ; Hematocrit ; Humans ; Limit of Detection ; Potassium/blood ; Theophylline/blood
Chemical Substances	Caffeine (3G6A5W338E) ; Theophylline (C137DTR5RG) ; 1,7-dimethylxanthine (Q3565Y41V7) ; Potassium (RWP5GA015D)
Language	English
Publishing date	2014-10
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-014-8114-z
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Article: Prediction of the Hematocrit of Dried Blood Spots via Potassium Measurement on a Routine Clinical Chemistry Analyzer

Capiau, Sara / Lambert Willy E / Stove Christophe P / Stove Veronique V

Analytical chemistry. 2013 Jan. 02, v. 85, no. 1

2013

Abstract	The potential of dried blood spot (DBS) sampling as an alternative for classical venous sampling is increasingly recognized, with multiple applications in, e.g., therapeutic drug monitoring and toxicology. Although DBS sampling has many advantages, it is associated with several issues, the hematocrit (Hct) issue being the most widely discussed challenge, given its possible strong impact on DBS-based quantitation. Hitherto, no approaches allow Hct prediction from nonvolumetrically applied DBS. Following a simple and rapid extraction protocol, Kâº levels from 3 mm DBS punches were measured via indirect potentiometry, using the Roche Cobas 8000 routine chemistry analyzer. The extractsâ Kâº concentrations were used to calculate the approximate Hct of the blood used to generate DBS. A linear calibration line was established, with a Hct range of 0.19 to 0.63 (lower limit of quantification, LLOQ, to upper limit of quantification, ULOQ). The procedure was fully validated; the bias and imprecision of quality controls (QCs) at three Hct levels and at the LLOQ and ULOQ was less than 5 and 12%, respectively. In addition, the influence of storage (pre- and postextraction), volume spotted, and punch homogeneity was evaluated. Application on DBS from patient samples (n = 111), followed by Bland and Altman, Passing and Bablok, and Deming regression analysis, demonstrated a good correlation between the âpredicted Hctâ and the âactual Hctâ. After correcting for the observed bias, limits of agreement of Â±0.049 were established. Incurred sample reanalysis demonstrated assay reproducibility. In conclusion, potassium levels in extracts from 3 mm DBS punches can be used to get a good prediction of the Hct, one of the most important âunknownsâ in DBS analysis.
Keywords	blood ; chemistry ; drugs ; hematocrit ; monitoring ; patients ; potassium ; prediction ; regression analysis ; toxicology
Language	English
Dates of publication	2013-0102
Size	p. 404-410.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/ac303014b
Database	NAL-Catalogue (AGRICOLA)

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Article: Potassium-based algorithm allows correction for the hematocrit bias in quantitative analysis of caffeine and its major metabolite in dried blood spots

De Kesel, Pieter M. M / Capiau, Sara / Stove, Veronique V / Lambert, Willy E / Stove, Christophe P

Analytical and bioanalytical chemistry. 2014 Oct., v. 406, no. 26

2014

Abstract	Although dried blood spot (DBS) sampling is increasingly receiving interest as a potential alternative to traditional blood sampling, the impact of hematocrit (Hct) on DBS results is limiting its final breakthrough in routine bioanalysis. To predict the Hct of a given DBS, potassium (K⁺) proved to be a reliable marker. The aim of this study was to evaluate whether application of an algorithm, based upon predicted Hct or K⁺concentrations as such, allowed correction for the Hct bias. Using validated LC-MS/MS methods, caffeine, chosen as a model compound, was determined in whole blood and corresponding DBS samples with a broad Hct range (0.18–0.47). A reference subset (n = 50) was used to generate an algorithm based on K⁺concentrations in DBS. Application of the developed algorithm on an independent test set (n = 50) alleviated the assay bias, especially at lower Hct values. Before correction, differences between DBS and whole blood concentrations ranged from −29.1 to 21.1 %. The mean difference, as obtained by Bland-Altman comparison, was −6.6 % (95 % confidence interval (CI), −9.7 to −3.4 %). After application of the algorithm, differences between corrected and whole blood concentrations lay between −19.9 and 13.9 % with a mean difference of −2.1 % (95 % CI, −4.5 to 0.3 %). The same algorithm was applied to a separate compound, paraxanthine, which was determined in 103 samples (Hct range, 0.17–0.47), yielding similar results. In conclusion, a K⁺-based algorithm allows correction for the Hct bias in the quantitative analysis of caffeine and its metabolite paraxanthine.
Keywords	algorithms ; blood ; blood sampling ; caffeine ; confidence interval ; hematocrit ; metabolites ; potassium ; quantitative analysis
Language	English
Dates of publication	2014-10
Size	p. 6749-6755.
Publishing place	Springer-Verlag
Document type	Article
ISSN	1618-2642
DOI	10.1007/s00216-014-8114-z
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Folates from metabolically engineered rice: a long-term study in rats.

Kiekens, Filip / Blancquaert, Dieter / Devisscher, Lindsey / Van Daele, Jeroen / Stove, Veronique V / Delanghe, Joris R / Van Der Straeten, Dominique / Lambert, Willy E / Stove, Christophe P

Molecular nutrition & food research

2015 Volume 59, Issue 3, Page(s) 490–500

Abstract: Scope: The biological impact of folates from folate rice, a metabolically engineered (biofortified) rice line, rich in folates, was investigated. Its consumption may be helpful to fight folate deficiency. Our objective was to investigate the potential ... ...

Abstract	Scope: The biological impact of folates from folate rice, a metabolically engineered (biofortified) rice line, rich in folates, was investigated. Its consumption may be helpful to fight folate deficiency. Our objective was to investigate the potential of folate rice to supply the organism with folates and evaluate its biological effectiveness using a rat model. Methods and results: Five groups of 12 Wistar rats were monitored during a 7/12-wk depletion/repletion trial. Animals receiving folate-free diet (0 μg/rat/day) and those additionally receiving wild-type rice (on average 0.11 μg/rat/day) suffered from decreased hematocrit and lower folate concentrations in both plasma and RBCs. This resulted in serious morbidity and even lethality during the trial. In contrast, all animals receiving a daily supplement of folate rice or folic acid fortified rice (on average 3.00 μg/rat/day and 3.12 μg/rat/day, respectively) and those receiving a positive control diet (11.4 to 25.0 μg/rat/day), survived. In these groups, the hematocrit normalized, plasma and RBC folate concentrations increased and pronounced hyperhomocysteinemia was countered. Conclusion: Using an animal model, we demonstrated that biofortified folate rice is a valuable source of dietary folate, as evidenced by folate determination in plasma and RBCs, the alleviation of anemia and counteraction of pronounced hyperhomocysteinemia.
MeSH term(s)	Animals ; Body Weight/drug effects ; Erythrocytes/drug effects ; Erythrocytes/metabolism ; Female ; Folic Acid/blood ; Folic Acid/pharmacology ; Food, Fortified ; Hematocrit ; Homocysteine/blood ; Longitudinal Studies ; Oryza/chemistry ; Oryza/metabolism ; Plants, Genetically Modified/chemistry ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/metabolism ; Rats, Wistar ; Vitamin B 12/blood
Chemical Substances	Homocysteine (0LVT1QZ0BA) ; Folic Acid (935E97BOY8) ; Vitamin B 12 (P6YC3EG204)
Language	English
Publishing date	2015-03
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2160372-8
ISSN	1613-4133 ; 1613-4125
ISSN (online)	1613-4133
ISSN	1613-4125
DOI	10.1002/mnfr.201400590
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Article: Folates from metabolically engineered rice: A long‐term study in rats

Kiekens, Filip / Blancquaert, Dieter / Devisscher, Lindsey / Daele, Jeroen / Stove, Veronique V. / Delanghe, Joris R. / Straeten, Dominique / Lambert, Willy E. / Stove, Christophe P.

Molecular nutrition & food research

Volume v. 59,, Issue no. 3

Abstract: SCOPE: The biological impact of folates from folate rice, a metabolically engineered (biofortified) rice line, rich in folates, was investigated. Its consumption may be helpful to fight folate deficiency. Our objective was to investigate the potential of ...

Abstract	SCOPE: The biological impact of folates from folate rice, a metabolically engineered (biofortified) rice line, rich in folates, was investigated. Its consumption may be helpful to fight folate deficiency. Our objective was to investigate the potential of folate rice to supply the organism with folates and evaluate its biological effectiveness using a rat model. METHODS AND RESULTS: Five groups of 12 Wistar rats were monitored during a 7/12‐wk depletion/repletion trial. Animals receiving folate‐free diet (0 μg/rat/day) and those additionally receiving wild‐type rice (on average 0.11 μg/rat/day) suffered from decreased hematocrit and lower folate concentrations in both plasma and RBCs. This resulted in serious morbidity and even lethality during the trial. In contrast, all animals receiving a daily supplement of folate rice or folic acid fortified rice (on average 3.00 μg/rat/day and 3.12 μg/rat/day, respectively) and those receiving a positive control diet (11.4 to 25.0 μg/rat/day), survived. In these groups, the hematocrit normalized, plasma and RBC folate concentrations increased and pronounced hyperhomocysteinemia was countered. CONCLUSION: Using an animal model, we demonstrated that biofortified folate rice is a valuable source of dietary folate, as evidenced by folate determination in plasma and RBCs, the alleviation of anemia and counteraction of pronounced hyperhomocysteinemia.
Keywords	folic acid ; repletion ; erythrocytes ; rice ; anemia ; diet ; metabolic engineering ; biofortification ; animal models ; hematocrit ; rats ; morbidity
Language	English
Document type	Article
ISSN	1613-4125
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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