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  1. AU="Stucke, Emily"
  2. AU="Lan, Qilong"
  3. AU="Hamamoto, Shoichiro"
  4. AU="Jaques, Guillaume"
  5. AU="Pasini, Davide"
  6. AU="Barbieri, Magali"
  7. AU="Kanizsai, Péter"
  8. AU="Altahawi, Faysal" AU="Altahawi, Faysal"
  9. AU="Rai, Anurag"
  10. AU="Udrea, Ana Maria"
  11. AU=Lo Giudice Roberto

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  1. Artikel ; Online: ApoE: A new piece to the severe malaria puzzle.

    Stucke, Emily M / Lawton, Jonathan G / Travassos, Mark A

    Pediatric research

    2024  

    Sprache Englisch
    Erscheinungsdatum 2024-02-22
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 4411-8
    ISSN 1530-0447 ; 0031-3998
    ISSN (online) 1530-0447
    ISSN 0031-3998
    DOI 10.1038/s41390-024-03096-5
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  2. Artikel ; Online: Gene expression analyses reveal differences in children's response to malaria according to their age.

    Tebben, Kieran / Yirampo, Salif / Coulibaly, Drissa / Koné, Abdoulaye K / Laurens, Matthew B / Stucke, Emily M / Dembélé, Ahmadou / Tolo, Youssouf / Traoré, Karim / Niangaly, Amadou / Berry, Andrea A / Kouriba, Bourema / Plowe, Christopher V / Doumbo, Ogobara K / Lyke, Kirsten E / Takala-Harrison, Shannon / Thera, Mahamadou A / Travassos, Mark A / Serre, David

    Nature communications

    2024  Band 15, Heft 1, Seite(n) 2021

    Abstract: In Bandiagara, Mali, children experience on average two clinical malaria episodes per year. However, even in the same transmission area, the number of uncomplicated symptomatic infections, and their parasitemia, can vary dramatically among children. We ... ...

    Abstract In Bandiagara, Mali, children experience on average two clinical malaria episodes per year. However, even in the same transmission area, the number of uncomplicated symptomatic infections, and their parasitemia, can vary dramatically among children. We simultaneously characterize host and parasite gene expression profiles from 136 Malian children with symptomatic falciparum malaria and examine differences in the relative proportion of immune cells and parasite stages, as well as in gene expression, associated with infection and or patient characteristics. Parasitemia explains much of the variation in host and parasite gene expression, and infections with higher parasitemia display proportionally more neutrophils and fewer T cells, suggesting parasitemia-dependent neutrophil recruitment and/or T cell extravasation to secondary lymphoid organs. The child's age also strongly correlates with variations in gene expression: Plasmodium falciparum genes associated with age suggest that older children carry more male gametocytes, while variations in host gene expression indicate a stronger innate response in younger children and stronger adaptive response in older children. These analyses highlight the variability in host responses and parasite regulation during P. falciparum symptomatic infections and emphasize the importance of considering the children's age when studying and treating malaria infections.
    Mesh-Begriff(e) Child ; Humans ; Male ; Adolescent ; Parasitemia/genetics ; Malaria ; Gene Expression Profiling ; Malaria, Falciparum/genetics ; Cell Movement
    Sprache Englisch
    Erscheinungsdatum 2024-03-06
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-46416-3
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  3. Artikel: Gene expression analyses reveal differences in children's response to malaria according to their age.

    Tebben, Kieran / Yirampo, Salif / Coulibaly, Drissa / Koné, Abdoulaye / Laurens, Matthew / Stucke, Emily / Dembélé, Ahmadou / Tolo, Youssouf / Traoré, Karim / Niangaly, Ahmadou / Berry, Andrea / Kouriba, Bourema / Plowe, Christopher / Doumbo, Ogobara / Lyke, Kirsten / Takala-Harrison, Shannon / Thera, Mahamadou / Travassos, Mark / Serre, David

    Research square

    2023  

    Abstract: In Bandiagara, Mali, children experience on average two clinical malaria episodes per season. However, even in the same transmission area, the number of uncomplicated symptomatic infections, and their parasitemia, vary dramatically among children. To ... ...

    Abstract In Bandiagara, Mali, children experience on average two clinical malaria episodes per season. However, even in the same transmission area, the number of uncomplicated symptomatic infections, and their parasitemia, vary dramatically among children. To examine the factors contributing to these variations, we simultaneously characterized the host and parasite gene expression profiles from 136 children with symptomatic falciparum malaria and analyzed the expression of 9,205 human and 2,484 Plasmodium genes. We used gene expression deconvolution to estimate the relative proportion of immune cells and parasite stages in each sample and to adjust the differential gene expression analyses. Parasitemia explained much of the variation in both host and parasite gene expression and revealed that infections with higher parasitemia had more neutrophils and fewer T cells, suggesting parasitemia-dependent neutrophil recruitment and/or T cell extravasation to secondary lymphoid organs. The child's age was also strongly correlated with gene expression variations. Plasmodium falciparum genes associated with age suggested that older children carried more male gametocytes, while host genes associated with age indicated a stronger innate response (through TLR and NLR signaling) in younger children and stronger adaptive immunity (through TCR and BCR signaling) in older children. These analyses highlight the variability in host responses and parasite regulation during P. falciparum symptomatic infections and emphasize the importance of considering the children's age when studying and treating malaria infections.
    Sprache Englisch
    Erscheinungsdatum 2023-10-27
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.21203/rs.3.rs-3487114/v1
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  4. Artikel ; Online: Clostridium difficile Colonization of Nursing Home Residents.

    Roghmann, Mary-Claire / Andronescu, Liana R / Stucke, Emily M / Johnson, J Kristie

    Infection control and hospital epidemiology

    2017  Band 38, Heft 10, Seite(n) 1267–1268

    Mesh-Begriff(e) Clostridium Infections/epidemiology ; Clostridium difficile/isolation & purification ; Humans ; Nursing Homes/statistics & numerical data ; Skin/microbiology ; United States/epidemiology
    Sprache Englisch
    Erscheinungsdatum 2017-08-22
    Erscheinungsland United States
    Dokumenttyp Letter ; Multicenter Study ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 639378-0
    ISSN 1559-6834 ; 0195-9417 ; 0899-823X
    ISSN (online) 1559-6834
    ISSN 0195-9417 ; 0899-823X
    DOI 10.1017/ice.2017.172
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  5. Artikel ; Online: Protein Microarrays as a Tool to Analyze Antibody Responses to Variant Surface Antigens Expressed on the Surface of Plasmodium falciparum-Infected Erythrocytes.

    Zhou, Albert E / Jain, Aarti / Nakajima, Rie / Shrestha, Biraj / Stucke, Emily M / Joshi, Sudhaunshu / Strauss, Kathy A / Hedde, Per N / Berry, Andrea A / Felgner, Philip L / Travassos, Mark A

    Methods in molecular biology (Clifton, N.J.)

    2022  Band 2470, Seite(n) 343–358

    Abstract: Enzyme-linked immunosorbent assays (ELISAs) remain the gold standard for measuring antibodies, but are time-consuming and use significant amounts of precious sample and reagents. Protein microarrays represent an appealing alternative, particularly for ... ...

    Abstract Enzyme-linked immunosorbent assays (ELISAs) remain the gold standard for measuring antibodies, but are time-consuming and use significant amounts of precious sample and reagents. Protein microarrays represent an appealing alternative, particularly for studies focused on large gene families such as those encoding variant surface antigens in the malaria parasite Plasmodium falciparum. Such microarrays represent an ideal high-throughput platform to study antibody responses to hundreds of malaria parasite variant surface antigens at once, providing critical insights into the development of natural immunity to malaria. We describe the essential background and approach to run an assay using a P. falciparum microarray populated with variant surface antigens. This allows the user to define serologic profiles and identify serodominant antigens that represent promising targets for vaccine or therapeutic development.
    Mesh-Begriff(e) Antibodies, Protozoan ; Antibody Formation ; Antigens, Protozoan ; Antigens, Surface/metabolism ; Erythrocytes/metabolism ; Humans ; Malaria ; Malaria, Falciparum/parasitology ; Plasmodium falciparum/metabolism ; Protein Array Analysis ; Protozoan Proteins/metabolism
    Chemische Substanzen Antibodies, Protozoan ; Antigens, Protozoan ; Antigens, Surface ; Protozoan Proteins
    Sprache Englisch
    Erscheinungsdatum 2022-07-25
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2189-9_25
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  6. Artikel ; Online: Burden of perianal

    Kim, Justin J / Johnson, J Kristie / Stucke, Emily M / Sorkin, John D / Zhao, LiCheng / Lydecker, Alison / Mody, Lona / Roghmann, Mary-Claire

    Infection control and hospital epidemiology

    2020  Band 41, Heft 12, Seite(n) 1396–1401

    Abstract: Objective: To evaluate the effect of the burden of Staphylococcus aureus colonization of nursing home residents on the risk of S. aureus transmission to healthcare worker (HCW) gowns and gloves.: Design: Multicenter prospective cohort study.: ... ...

    Abstract Objective: To evaluate the effect of the burden of Staphylococcus aureus colonization of nursing home residents on the risk of S. aureus transmission to healthcare worker (HCW) gowns and gloves.
    Design: Multicenter prospective cohort study.
    Setting and participants: Residents and HCWs from 13 community-based nursing homes in Maryland and Michigan.
    Methods: Residents were cultured for S. aureus at the anterior nares and perianal skin. The S. aureus burden was estimated by quantitative polymerase chain reaction detecting the nuc gene. HCWs wore gowns and gloves during usual care activities; gowns and gloves were swabbed and then cultured for the presence of S. aureus.
    Results: In total, 403 residents were enrolled; 169 were colonized with methicillin-resistant S. aureus (MRSA) or methicillin-sensitive S. aureus (MSSA) and comprised the study population; 232 were not colonized and thus were excluded from this analysis; and 2 were withdrawn prior to being swabbed. After multivariable analysis, perianal colonization with S. aureus conferred the greatest odds for transmission to HCW gowns and gloves, and the odds increased with increasing burden of colonization: adjusted odds ratio (aOR), 2.1 (95% CI, 1.3-3.5) for low-level colonization and aOR 5.2 (95% CI, 3.1-8.7) for high level colonization.
    Conclusions: Among nursing home patients colonized with S. aureus, the risk of transmission to HCW gowns and gloves was greater from those colonized with greater quantities of S. aureus on the perianal skin. Our findings inform future infection control practices for both MRSA and MSSA in nursing homes.
    Mesh-Begriff(e) Cross Infection/epidemiology ; Health Personnel ; Humans ; Methicillin-Resistant Staphylococcus aureus ; Nursing Homes ; Prospective Studies ; Staphylococcal Infections/epidemiology ; Staphylococcus aureus
    Sprache Englisch
    Erscheinungsdatum 2020-08-07
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Multicenter Study ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 639378-0
    ISSN 1559-6834 ; 0195-9417 ; 0899-823X
    ISSN (online) 1559-6834
    ISSN 0195-9417 ; 0899-823X
    DOI 10.1017/ice.2020.336
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  7. Artikel ; Online: Malian children infected with Plasmodium ovale and Plasmodium falciparum display very similar gene expression profiles.

    Tebben, Kieran / Yirampo, Salif / Coulibaly, Drissa / Koné, Abdoulaye K / Laurens, Matthew B / Stucke, Emily M / Dembélé, Ahmadou / Tolo, Youssouf / Traoré, Karim / Niangaly, Amadou / Berry, Andrea A / Kouriba, Bourema / Plowe, Christopher V / Doumbo, Ogobara K / Lyke, Kirsten E / Takala-Harrison, Shannon / Thera, Mahamadou A / Travassos, Mark A / Serre, David

    PLoS neglected tropical diseases

    2023  Band 17, Heft 1, Seite(n) e0010802

    Abstract: Plasmodium parasites caused 241 million cases of malaria and over 600,000 deaths in 2020. Both P. falciparum and P. ovale are endemic to Mali and cause clinical malaria, with P. falciparum infections typically being more severe. Here, we sequenced RNA ... ...

    Abstract Plasmodium parasites caused 241 million cases of malaria and over 600,000 deaths in 2020. Both P. falciparum and P. ovale are endemic to Mali and cause clinical malaria, with P. falciparum infections typically being more severe. Here, we sequenced RNA from nine pediatric blood samples collected during infections with either P. falciparum or P. ovale, and characterized the host and parasite gene expression profiles. We found that human gene expression varies more between individuals than according to the parasite species causing the infection, while parasite gene expression profiles cluster by species. Additionally, we characterized DNA polymorphisms of the parasites directly from the RNA-seq reads and found comparable levels of genetic diversity in both species, despite dramatic differences in prevalence. Our results provide unique insights into host-pathogen interactions during malaria infections and their variations according to the infecting Plasmodium species, which will be critical to develop better elimination strategies against all human Plasmodium parasites.
    Mesh-Begriff(e) Child ; Humans ; Malaria/epidemiology ; Malaria/genetics ; Malaria, Falciparum/epidemiology ; Malaria, Falciparum/genetics ; Plasmodium falciparum ; Plasmodium ovale ; Transcriptome
    Sprache Englisch
    Erscheinungsdatum 2023-01-25
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2429704-5
    ISSN 1935-2735 ; 1935-2735
    ISSN (online) 1935-2735
    ISSN 1935-2735
    DOI 10.1371/journal.pntd.0010802
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  8. Artikel: Gene expression analyses reveal differences in children's response to malaria according to their age.

    Tebben, Kieran / Yirampo, Salif / Coulibaly, Drissa / Koné, Abdoulaye K / Laurens, Matthew B / Stucke, Emily M / Dembélé, Ahmadou / Tolo, Youssouf / Traoré, Karim / Niangaly, Amadou / Berry, Andrea A / Kouriba, Bourema / Plowe, Christopher V / Doumbo, Ogobara K / Lyke, Kirsten E / Takala-Harrison, Shannon / Thera, Mahamadou A / Travassos, Mark A / Serre, David

    bioRxiv : the preprint server for biology

    2023  

    Abstract: In Bandiagara, Mali, children experience on average two clinical malaria episodes per season. However, even in the same transmission area, the number of uncomplicated symptomatic infections, and their parasitemia, vary dramatically among children. To ... ...

    Abstract In Bandiagara, Mali, children experience on average two clinical malaria episodes per season. However, even in the same transmission area, the number of uncomplicated symptomatic infections, and their parasitemia, vary dramatically among children. To examine the factors contributing to these variations, we simultaneously characterized the host and parasite gene expression profiles from 136 children with symptomatic falciparum malaria and analyzed the expression of 9,205 human and 2,484
    Sprache Englisch
    Erscheinungsdatum 2023-10-26
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2023.10.24.563751
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  9. Artikel ; Online: Optimization of parasite DNA enrichment approaches to generate whole genome sequencing data for Plasmodium falciparum from low parasitaemia samples.

    Shah, Zalak / Adams, Matthew / Moser, Kara A / Shrestha, Biraj / Stucke, Emily M / Laufer, Miriam K / Serre, David / Silva, Joana C / Takala-Harrison, Shannon

    Malaria journal

    2020  Band 19, Heft 1, Seite(n) 135

    Abstract: Background: Owing to the large amount of host DNA in clinical samples, generation of high-quality Plasmodium falciparum whole genome sequencing (WGS) data requires enrichment for parasite DNA. Enrichment is often achieved by leukocyte depletion of ... ...

    Abstract Background: Owing to the large amount of host DNA in clinical samples, generation of high-quality Plasmodium falciparum whole genome sequencing (WGS) data requires enrichment for parasite DNA. Enrichment is often achieved by leukocyte depletion of infected blood prior to storage. However, leukocyte depletion is difficult in low-resource settings and limits analysis to prospectively-collected samples. As a result, approaches such as selective whole genome amplification (sWGA) are being used to enrich for parasite DNA. However, sWGA has had limited success in generating reliable sequencing data from low parasitaemia samples. In this study, enzymatic digestion with MspJI prior to sWGA and whole genome sequencing was evaluated to determine whether this approach improved genome coverage compared to sWGA alone. The potential of sWGA to cause amplification bias in polyclonal infections was also examined.
    Methods: DNA extracted from laboratory-created dried blood spots was treated with a modification-dependent restriction endonuclease, MspJI, and filtered via vacuum filtration. Samples were then selectively amplified using a previously reported sWGA protocol and subjected to WGS. Genome coverage statistics were compared between the optimized sWGA approach and the previously reported sWGA approach performed in parallel. Differential amplification by sWGA was assessed by comparing WGS data generated from lab-created mixtures of parasite isolates, from the same geographical region, generated with or without sWGA.
    Results: MspJI digestion did not enrich for parasite DNA. Samples that underwent vacuum filtration (without MspJI digestion) prior to sWGA had the highest parasite DNA concentration and displayed greater genome coverage compared to MspJI + sWGA and sWGA alone, particularly for low parasitaemia samples. The optimized sWGA (filtration + sWGA) approach was successfully used to generate WGS data from 218 non-leukocyte depleted field samples from Malawi. Sequences from lab-created mixtures of parasites did not show evidence of differential amplification of parasite strains compared to directly sequenced samples.
    Conclusion: This optimized sWGA approach is a reliable method to obtain WGS data from non-leukocyte depleted, low parasitaemia samples. The absence of amplification bias in data generated from mixtures of isolates from the same geographic region suggests that this approach can be appropriately used for molecular epidemiological studies.
    Mesh-Begriff(e) DNA, Protozoan/analysis ; Malawi ; Parasitemia/parasitology ; Plasmodium falciparum/genetics ; Whole Genome Sequencing/instrumentation ; Whole Genome Sequencing/methods
    Chemische Substanzen DNA, Protozoan
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-03-30
    Erscheinungsland England
    Dokumenttyp Journal Article
    ISSN 1475-2875
    ISSN (online) 1475-2875
    DOI 10.1186/s12936-020-03195-8
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  10. Artikel ; Online: Malian adults maintain serologic responses to virulent PfEMP1s amid seasonal patterns of fluctuation.

    Ventimiglia, Noah T / Stucke, Emily M / Coulibaly, Drissa / Berry, Andrea A / Lyke, Kirsten E / Laurens, Matthew B / Bailey, Jason A / Adams, Matthew / Niangaly, Amadou / Kone, Abdoulaye K / Takala-Harrison, Shannon / Kouriba, Bourema / Doumbo, Ogobara K / Felgner, Phillip L / Plowe, Christopher V / Thera, Mahamadou A / Travassos, Mark A

    Scientific reports

    2021  Band 11, Heft 1, Seite(n) 14401

    Abstract: Plasmodium falciparum erythrocyte membrane protein-1s (PfEMP1s), diverse malaria proteins expressed on the infected erythrocyte surface, play an important role in pathogenesis, mediating adhesion to host vascular endothelium. Antibodies to particular non- ...

    Abstract Plasmodium falciparum erythrocyte membrane protein-1s (PfEMP1s), diverse malaria proteins expressed on the infected erythrocyte surface, play an important role in pathogenesis, mediating adhesion to host vascular endothelium. Antibodies to particular non-CD36-binding PfEMP1s are associated with protection against severe disease. We hypothesized that given lifelong P. falciparum exposure, Malian adults would have broad PfEMP1 serorecognition and high seroreactivity levels during follow-up, particularly to non-CD36-binding PfEMP1s such as those that attach to endothelial protein C receptor (EPCR) and intercellular adhesion molecule-1 (ICAM-1). Using a protein microarray, we determined serologic responses to 166 reference PfEMP1 fragments during a dry and subsequent malaria transmission season in Malian adults. Malian adult sera had PfEMP1 serologic responses throughout the year, with decreased reactivity to a small subset of PfEMP1 fragments during the dry season and increases in reactivity to a different subset of PfEMP1 fragments during the subsequent peak malaria transmission season, especially for intracellular PfEMP1 domains. For some individuals, PfEMP1 serologic responses increased after the dry season, suggesting antigenic switching during asymptomatic infection. Adults were more likely to experience variable serorecognition of CD36-binding PfEMP1s than non-CD36-binding PfEMP1s that bind EPCR or ICAM-1, which remained serorecognized throughout the year. Sustained seroreactivity to non-CD36-binding PfEMP1s throughout adulthood amid seasonal fluctuation patterns may reflect underlying protective severe malaria immunity and merits further investigation.
    Mesh-Begriff(e) Adult ; Erythrocyte Membrane/metabolism ; Humans ; Plasmodium falciparum ; Protozoan Proteins/metabolism ; Seasons
    Chemische Substanzen Protozoan Proteins
    Sprache Englisch
    Erscheinungsdatum 2021-07-13
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-92974-7
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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