LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 22

Search options

  1. Article ; Online: Metaproteomics of Colonic Microbiota Unveils Discrete Protein Functions among Colitic Mice and Control Groups.

    Moon, Clara / Stupp, Gregory S / Su, Andrew I / Wolan, Dennis W

    Proteomics

    2018  Volume 18, Issue 3-4

    Abstract: Metaproteomics can greatly assist established high-throughput sequencing methodologies to provide systems biological insights into the alterations of microbial protein functionalities correlated with disease-associated dysbiosis of the intestinal ... ...

    Abstract Metaproteomics can greatly assist established high-throughput sequencing methodologies to provide systems biological insights into the alterations of microbial protein functionalities correlated with disease-associated dysbiosis of the intestinal microbiota. Here, the authors utilize the well-characterized murine T cell transfer model of colitis to find specific changes within the intestinal luminal proteome associated with inflammation. MS proteomic analysis of colonic samples permitted the identification of ≈10 000-12 000 unique peptides that corresponded to 5610 protein clusters identified across three groups, including the colitic Rag1
    MeSH term(s) Animals ; Bacterial Proteins/metabolism ; Colitis/metabolism ; Colitis/microbiology ; Colon/metabolism ; Colon/microbiology ; Disease Models, Animal ; Inflammation/metabolism ; Inflammation/microbiology ; Mice ; Mice, Inbred C57BL ; Microbiota ; Proteome/analysis
    Chemical Substances Bacterial Proteins ; Proteome
    Language English
    Publishing date 2018-02-02
    Publishing country Germany
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2032093-0
    ISSN 1615-9861 ; 1615-9853
    ISSN (online) 1615-9861
    ISSN 1615-9853
    DOI 10.1002/pmic.201700391
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5386: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.A 1868: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Quantitative Metaproteomics and Activity-based Protein Profiling of Patient Fecal Microbiome Identifies Host and Microbial Serine-type Endopeptidase Activity Associated With Ulcerative Colitis.

    Thuy-Boun, Peter S / Wang, Ana Y / Crissien-Martinez, Ana / Xu, Janice H / Chatterjee, Sandip / Stupp, Gregory S / Su, Andrew I / Coyle, Walter J / Wolan, Dennis W

    Molecular & cellular proteomics : MCP

    2022  Volume 21, Issue 3, Page(s) 100197

    Abstract: The gut microbiota plays an important yet incompletely understood role in the induction and propagation of ulcerative colitis (UC). Organism-level efforts to identify UC-associated microbes have revealed the importance of community structure, but less is ...

    Abstract The gut microbiota plays an important yet incompletely understood role in the induction and propagation of ulcerative colitis (UC). Organism-level efforts to identify UC-associated microbes have revealed the importance of community structure, but less is known about the molecular effectors of disease. We performed 16S rRNA gene sequencing in parallel with label-free data-dependent LC-MS/MS proteomics to characterize the stool microbiomes of healthy (n = 8) and UC (n = 10) patients. Comparisons of taxonomic composition between techniques revealed major differences in community structure partially attributable to the additional detection of host, fungal, viral, and food peptides by metaproteomics. Differential expression analysis of metaproteomic data identified 176 significantly enriched protein groups between healthy and UC patients. Gene ontology analysis revealed several enriched functions with serine-type endopeptidase activity overrepresented in UC patients. Using a biotinylated fluorophosphonate probe and streptavidin-based enrichment, we show that serine endopeptidases are active in patient fecal samples and that additional putative serine hydrolases are detectable by this approach compared with unenriched profiling. Finally, as metaproteomic databases expand, they are expected to asymptotically approach completeness. Using ComPIL and de novo peptide sequencing, we estimate the size of the probable peptide space unidentified ("dark peptidome") by our large database approach to establish a rough benchmark for database sufficiency. Despite high variability inherent in patient samples, our analysis yielded a catalog of differentially enriched proteins between healthy and UC fecal proteomes. This catalog provides a clinically relevant jumping-off point for further molecular-level studies aimed at identifying the microbial underpinnings of UC.
    MeSH term(s) Chromatography, Liquid ; Colitis, Ulcerative/diagnosis ; Colitis, Ulcerative/microbiology ; Endopeptidases ; Feces/microbiology ; Humans ; Microbiota ; RNA, Ribosomal, 16S/genetics ; Serine ; Tandem Mass Spectrometry
    Chemical Substances RNA, Ribosomal, 16S ; Serine (452VLY9402) ; Endopeptidases (EC 3.4.-)
    Language English
    Publishing date 2022-01-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2075924-1
    ISSN 1535-9484 ; 1535-9476
    ISSN (online) 1535-9484
    ISSN 1535-9476
    DOI 10.1016/j.mcpro.2022.100197
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5599: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Structured reviews for data and knowledge-driven research.

    Queralt-Rosinach, Núria / Stupp, Gregory S / Li, Tong Shu / Mayers, Michael / Hoatlin, Maureen E / Might, Matthew / Good, Benjamin M / Su, Andrew I

    Database : the journal of biological databases and curation

    2020  Volume 2020

    Abstract: Hypothesis generation is a critical step in research and a cornerstone in the rare disease field. Research is most efficient when those hypotheses are based on the entirety of knowledge known to date. Systematic review articles are commonly used in ... ...

    Abstract Hypothesis generation is a critical step in research and a cornerstone in the rare disease field. Research is most efficient when those hypotheses are based on the entirety of knowledge known to date. Systematic review articles are commonly used in biomedicine to summarize existing knowledge and contextualize experimental data. But the information contained within review articles is typically only expressed as free-text, which is difficult to use computationally. Researchers struggle to navigate, collect and remix prior knowledge as it is scattered in several silos without seamless integration and access. This lack of a structured information framework hinders research by both experimental and computational scientists. To better organize knowledge and data, we built a structured review article that is specifically focused on NGLY1 Deficiency, an ultra-rare genetic disease first reported in 2012. We represented this structured review as a knowledge graph and then stored this knowledge graph in a Neo4j database to simplify dissemination, querying and visualization of the network. Relative to free-text, this structured review better promotes the principles of findability, accessibility, interoperability and reusability (FAIR). In collaboration with domain experts in NGLY1 Deficiency, we demonstrate how this resource can improve the efficiency and comprehensiveness of hypothesis generation. We also developed a read-write interface that allows domain experts to contribute FAIR structured knowledge to this community resource. In contrast to traditional free-text review articles, this structured review exists as a living knowledge graph that is curated by humans and accessible to computational analyses. Finally, we have generalized this workflow into modular and repurposable components that can be applied to other domain areas. This NGLY1 Deficiency-focused network is publicly available at http://ngly1graph.org/.
    Availability and implementation: Database URL: http://ngly1graph.org/. Network data files are at: https://github.com/SuLab/ngly1-graph and source code at: https://github.com/SuLab/bioknowledge-reviewer.
    Contact: asu@scripps.edu.
    MeSH term(s) Animals ; Biomedical Research/methods ; Biomedical Research/statistics & numerical data ; Computational Biology/methods ; Computational Biology/statistics & numerical data ; Congenital Disorders of Glycosylation/genetics ; Congenital Disorders of Glycosylation/metabolism ; Data Curation/methods ; Data Mining/methods ; Databases, Factual ; Humans ; Internet ; Knowledge Bases ; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase/deficiency ; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase/genetics ; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase/metabolism ; Systematic Reviews as Topic
    Chemical Substances Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase (EC 3.5.1.52)
    Language English
    Publishing date 2020-03-19
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2496706-3
    ISSN 1758-0463 ; 1758-0463
    ISSN (online) 1758-0463
    ISSN 1758-0463
    DOI 10.1093/database/baaa015
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Quantitative Metaproteomics and Activity-Based Probe Enrichment Reveals Significant Alterations in Protein Expression from a Mouse Model of Inflammatory Bowel Disease

    Mayers, MichaelD / Moon Clara / Stupp Gregory S / Su Andrew I / Wolan Dennis W

    Journal of Proteome Research. 2017 Feb. 03, v. 16, no. 2

    2017  

    Abstract: Tandem mass spectrometry based shotgun proteomics of distal gut microbiomes is exceedingly difficult due to the inherent complexity and taxonomic diversity of the samples. We introduce two new methodologies to improve metaproteomic studies of microbiome ... ...

    Abstract Tandem mass spectrometry based shotgun proteomics of distal gut microbiomes is exceedingly difficult due to the inherent complexity and taxonomic diversity of the samples. We introduce two new methodologies to improve metaproteomic studies of microbiome samples. These methods include the stable isotope labeling in mammals to permit protein quantitation across two mouse cohorts as well as the application of activity-based probes to enrich and analyze both host and microbial proteins with specific functionalities. We used these technologies to study the microbiota from the adoptive T cell transfer mouse model of inflammatory bowel disease (IBD) and compare these samples to an isogenic control, thereby limiting genetic and environmental variables that influence microbiome composition. The data generated highlight quantitative alterations in both host and microbial proteins due to intestinal inflammation and corroborates the observed phylogenetic changes in bacteria that accompany IBD in humans and mouse models. The combination of isotope labeling with shotgun proteomics resulted in the total identification of 4434 protein clusters expressed in the microbial proteomic environment, 276 of which demonstrated differential abundance between control and IBD mice. Notably, application of a novel cysteine-reactive probe uncovered several microbial proteases and hydrolases overrepresented in the IBD mice. Implementation of these methods demonstrated that substantial insights into the identity and dysregulation of host and microbial proteins altered in IBD can be accomplished and can be used in the interrogation of other microbiome-related diseases.
    Keywords T-lymphocytes ; animal models ; bacteria ; digestive system ; environmental factors ; humans ; inflammation ; inflammatory bowel disease ; isotope labeling ; mice ; microbial proteins ; microbiome ; phylogeny ; protein synthesis ; proteinases ; proteome ; proteomics ; species diversity ; stable isotopes ; tandem mass spectrometry
    Language English
    Dates of publication 2017-0203
    Size p. 1014-1026.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 2078618-9
    ISSN 1535-3907 ; 1535-3893
    ISSN (online) 1535-3907
    ISSN 1535-3893
    DOI 10.1021%2Facs.jproteome.6b00938
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6189: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Triflic Acid Treatment Enables LC-MS/MS Analysis of Insoluble Bacterial Biomass.

    Wang, Ana Y / Thuy-Boun, Peter S / Stupp, Gregory S / Su, Andrew I / Wolan, Dennis W

    Journal of proteome research

    2018  Volume 17, Issue 9, Page(s) 2978–2986

    Abstract: The lysis and extraction of soluble bacterial proteins from cells is a common practice for proteomics analyses, but insoluble bacterial biomasses are often left behind. Here, we show that with triflic acid treatment, the insoluble bacterial biomass of ... ...

    Abstract The lysis and extraction of soluble bacterial proteins from cells is a common practice for proteomics analyses, but insoluble bacterial biomasses are often left behind. Here, we show that with triflic acid treatment, the insoluble bacterial biomass of Gram
    MeSH term(s) Bacillus subtilis/chemistry ; Bacillus subtilis/genetics ; Bacillus subtilis/metabolism ; Bacterial Proteins/isolation & purification ; Chromatography, Liquid ; Complex Mixtures/chemistry ; Gastrointestinal Microbiome/genetics ; Humans ; Jurkat Cells ; Membrane Proteins/isolation & purification ; Mesylates/chemistry ; Metagenome ; Proteomics/methods ; Pseudomonas aeruginosa/chemistry ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/metabolism ; Sonication/methods ; Tandem Mass Spectrometry
    Chemical Substances Bacterial Proteins ; Complex Mixtures ; Membrane Proteins ; Mesylates ; trifluoromethanesulfonic acid (JE2SY203E8)
    Language English
    Publishing date 2018-08-08
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2078618-9
    ISSN 1535-3907 ; 1535-3893
    ISSN (online) 1535-3907
    ISSN 1535-3893
    DOI 10.1021/acs.jproteome.8b00166
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6189: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article: Triflic Acid Treatment Enables LC-MS/MS Analysis of Insoluble Bacterial Biomass

    Wang, Ana Y / Thuy-Boun, Peter S / Stupp, Gregory S / Su, Andrew I / Wolan, Dennis W

    Journal of proteome research. 2018 July 18, v. 17, no. 9

    2018  

    Abstract: The lysis and extraction of soluble bacterial proteins from cells is a common practice for proteomics analyses, but insoluble bacterial biomasses are often left behind. Here, we show that with triflic acid treatment, the insoluble bacterial biomass of ... ...

    Abstract The lysis and extraction of soluble bacterial proteins from cells is a common practice for proteomics analyses, but insoluble bacterial biomasses are often left behind. Here, we show that with triflic acid treatment, the insoluble bacterial biomass of Gram– and Gram+ bacteria can be rendered soluble. We use LC-MS/MS shotgun proteomics to show that bacterial proteins in the soluble and insoluble postlysis fractions differ significantly. Additionally, in the case of Gram– Pseudomonas aeruginosa, triflic acid treatment enables the enrichment of cell-envelope-associated proteins. Finally, we apply triflic acid to a human microbiome sample to show that this treatment is robust and enables the identification of a new, complementary subset of proteins from a complex microbial mixture.
    Keywords Pseudomonas aeruginosa ; acid treatment ; bacteria ; bacterial biomass ; bacterial proteins ; humans ; liquid chromatography ; microbiome ; proteome ; proteomics ; tandem mass spectrometry
    Language English
    Dates of publication 2018-0718
    Size p. 2978-2986.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 2078618-9
    ISSN 1535-3907 ; 1535-3893
    ISSN (online) 1535-3907
    ISSN 1535-3893
    DOI 10.1021/acs.jproteome.8b00166
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6189: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Quantitative Metaproteomics and Activity-Based Probe Enrichment Reveals Significant Alterations in Protein Expression from a Mouse Model of Inflammatory Bowel Disease.

    Mayers, Michael D / Moon, Clara / Stupp, Gregory S / Su, Andrew I / Wolan, Dennis W

    Journal of proteome research

    2017  Volume 16, Issue 2, Page(s) 1014–1026

    Abstract: Tandem mass spectrometry based shotgun proteomics of distal gut microbiomes is exceedingly difficult due to the inherent complexity and taxonomic diversity of the samples. We introduce two new methodologies to improve metaproteomic studies of microbiome ... ...

    Abstract Tandem mass spectrometry based shotgun proteomics of distal gut microbiomes is exceedingly difficult due to the inherent complexity and taxonomic diversity of the samples. We introduce two new methodologies to improve metaproteomic studies of microbiome samples. These methods include the stable isotope labeling in mammals to permit protein quantitation across two mouse cohorts as well as the application of activity-based probes to enrich and analyze both host and microbial proteins with specific functionalities. We used these technologies to study the microbiota from the adoptive T cell transfer mouse model of inflammatory bowel disease (IBD) and compare these samples to an isogenic control, thereby limiting genetic and environmental variables that influence microbiome composition. The data generated highlight quantitative alterations in both host and microbial proteins due to intestinal inflammation and corroborates the observed phylogenetic changes in bacteria that accompany IBD in humans and mouse models. The combination of isotope labeling with shotgun proteomics resulted in the total identification of 4434 protein clusters expressed in the microbial proteomic environment, 276 of which demonstrated differential abundance between control and IBD mice. Notably, application of a novel cysteine-reactive probe uncovered several microbial proteases and hydrolases overrepresented in the IBD mice. Implementation of these methods demonstrated that substantial insights into the identity and dysregulation of host and microbial proteins altered in IBD can be accomplished and can be used in the interrogation of other microbiome-related diseases.
    MeSH term(s) Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/isolation & purification ; Bacterial Proteins/metabolism ; Chromatography, Liquid ; Disease Models, Animal ; Feces/microbiology ; Female ; Gastrointestinal Microbiome/genetics ; Gene Deletion ; Gene Expression ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Humans ; Inflammatory Bowel Diseases/genetics ; Inflammatory Bowel Diseases/microbiology ; Inflammatory Bowel Diseases/pathology ; Intestines/microbiology ; Intestines/pathology ; Isotope Labeling ; Metagenome ; Mice ; Proteome/genetics ; Proteome/isolation & purification ; Proteome/metabolism ; Tandem Mass Spectrometry
    Chemical Substances Bacterial Proteins ; Homeodomain Proteins ; Proteome ; RAG-1 protein (128559-51-3)
    Language English
    Publishing date 2017-02-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2078618-9
    ISSN 1535-3907 ; 1535-3893
    ISSN (online) 1535-3907
    ISSN 1535-3893
    DOI 10.1021/acs.jproteome.6b00938
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6189: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Metabolomics and Natural-Products Strategies to Study Chemical Ecology in Nematodes.

    Edison, Arthur S / Clendinen, Chaevien S / Ajredini, Ramadan / Beecher, Chris / Ponce, Francesca V / Stupp, Gregory S

    Integrative and comparative biology

    2015  Volume 55, Issue 3, Page(s) 478–485

    Abstract: This review provides an overview of two complementary approaches to identify biologically active compounds for studies in chemical ecology. The first is activity-guided fractionation and the second is metabolomics, particularly focusing on a new liquid ... ...

    Abstract This review provides an overview of two complementary approaches to identify biologically active compounds for studies in chemical ecology. The first is activity-guided fractionation and the second is metabolomics, particularly focusing on a new liquid chromatography-mass spectrometry-based method called isotopic ratio outlier analysis. To illustrate examples using these approaches, we review recent experiments using Caenorhabditis elegans and related free-living nematodes.
    MeSH term(s) Animals ; Biological Products/metabolism ; Caenorhabditis elegans/physiology ; Chemical Fractionation/methods ; Chemotaxis ; Chromatography, Liquid ; Mass Spectrometry ; Metabolomics/methods ; Nematoda/physiology
    Chemical Substances Biological Products
    Language English
    Publishing date 2015-07-02
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2159110-6
    ISSN 1557-7023 ; 1540-7063
    ISSN (online) 1557-7023
    ISSN 1540-7063
    DOI 10.1093/icb/icv077
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article: 13

    Clendinen, Chaevien S / Stupp, Gregory S / Wang, Bing / Garrett, Timothy J / Edison, Arthur S

    Current Metabolomics

    2015  Volume 4, Issue 2, Page(s) 116–120

    Abstract: Abstract background: Isotopic Ratio Outlier Analysis (IROA) is an untargeted metabolomics method that uses stable isotopic labeling and LC-HRMS for identification and relative quantification of metabolites in a biological sample under varying ... ...

    Abstract Abstract background: Isotopic Ratio Outlier Analysis (IROA) is an untargeted metabolomics method that uses stable isotopic labeling and LC-HRMS for identification and relative quantification of metabolites in a biological sample under varying experimental conditions.
    Objective: We demonstrate a method using high-sensitivity
    Methods: IROA samples from the nematode Caenorhabditis elegans were fractionated using LC-HRMS using 5 repeated injections and collecting 30 sec fractions. These were concentrated and analyzed by
    Results: We isotopically labeled samples of C. elegans and collected 2 adjacent LC fractions. By HRMS, one contained at least 2 known metabolites, phenylalanine and inosine, and the other contained tryptophan and an unknown feature with a monoisotopic mass of m/z 380.0742 [M+H]
    Conclusion: We were able to dereplicate previously known metabolites and identify a metabolite that was not in databases by matching resonances to NMR databases and using chemical shift calculations to determine the correct isomer. This approach is efficient and can be used to identify unknown compounds of interest using the same material used for IROA.
    Language English
    Publishing date 2015-05-22
    Publishing country United Arab Emirates
    Document type Journal Article
    ISSN 2213-235X
    ISSN 2213-235X
    DOI 10.2174/2213235X04666160407212156
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article ; Online: ChlamBase: a curated model organism database for the Chlamydia research community.

    Putman, Tim / Hybiske, Kevin / Jow, Derek / Afrasiabi, Cyrus / Lelong, Sebastien / Cano, Marco Alvarado / Stupp, Gregory S / Waagmeester, Andra / Good, Benjamin M / Wu, Chunlei / Su, Andrew I

    Database : the journal of biological databases and curation

    2019  Volume 2019

    Language English
    Publishing date 2019-05-14
    Publishing country England
    Document type Journal Article ; Published Erratum
    ZDB-ID 2496706-3
    ISSN 1758-0463 ; 1758-0463
    ISSN (online) 1758-0463
    ISSN 1758-0463
    DOI 10.1093/database/baz091
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top