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Article ; Online: CSF N-Glycomics Using High-Throughput UPLC-ESI Techniques in Alzheimer's Disease.

Messina, Angela / Barone, Rita / Sturiale, Luisa / Zappia, Mario / Palmigiano, Angelo / Garozzo, Domenico

Methods in molecular biology (Clifton, N.J.)

2024 Volume 2785, Page(s) 37–48

Abstract: In this chapter, we will present a high-throughput method applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome. This methodology is based on a commercial equipment developed by WATERS™ to speed up N- ... ...

Abstract	In this chapter, we will present a high-throughput method applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome. This methodology is based on a commercial equipment developed by WATERS™ to speed up N-deglycosylation and N-glycan labeling of glycoproteins of pharmaceutical and biological interest such as monoclonal antibodies. This analytical kit is sold under the trade name of RapiFluor-MS (RFMS). We have slightly modified the methodology, increasing the glycosylation time and using a high-resolution mass analyzer for the analysis of CSF N-glycans, thus obtaining a high-throughput method (up to 96 samples simultaneously), mass accuracy better than 5 ppm, and the ability to separate and identify isomers.
MeSH term(s)	Humans ; Chromatography, High Pressure Liquid ; Glycomics/methods ; Alzheimer Disease/cerebrospinal fluid ; Glycosylation ; Glycoproteins/chemistry ; Polysaccharides/chemistry
Chemical Substances	Glycoproteins ; Polysaccharides
Language	English
Publishing date	2024-03-01
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3774-6_3
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: CSF N-Glycomics Using MALDI MS Techniques.

Messina, Angela / Romeo, Donata Agata / Barone, Rita / Sturiale, Luisa / Palmigiano, Angelo / Zappia, Mario / Garozzo, Domenico

Methods in molecular biology (Clifton, N.J.)

2024 Volume 2785, Page(s) 49–65

Abstract: In this chapter, we will present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome. N-glycans are released from denatured carboxymethylated glycoproteins by digestion with ... ...

Abstract	In this chapter, we will present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome. N-glycans are released from denatured carboxymethylated glycoproteins by digestion with peptide-N-glycosidase F (PNGase F) and purified using both C18 Sep-Pak
MeSH term(s)	Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Glycomics/methods ; Tandem Mass Spectrometry ; Glycoproteins/chemistry ; Glycosylation ; Polysaccharides/chemistry
Chemical Substances	Glycoproteins ; Polysaccharides
Language	English
Publishing date	2024-03-01
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3774-6_4
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Article: The Digital Applications of “Agriculture 4.0”: Strategic Opportunity for the Development of the Italian Citrus Chain

Scuderi, Alessandro / La Via, Giovanni / Timpanaro, Giuseppe / Sturiale, Luisa

Agriculture (Basel). 2022 Mar. 12, v. 12, no. 3

2022

Abstract: Contemporary agriculture is increasingly oriented toward the synergistic adoption of technologies such as the Internet of Things, Internet of Farming, big data analytics, and blockchain to combine resource protection and economic, social, and ... ...

Abstract	Contemporary agriculture is increasingly oriented toward the synergistic adoption of technologies such as the Internet of Things, Internet of Farming, big data analytics, and blockchain to combine resource protection and economic, social, and environmental sustainability. In Italy, the market growth potential of “Agriculture 4.0” and “Farming 4.0” solutions is very high, but the adoption of the related technological innovations is still low. Italian companies are increasingly aware of the opportunities offered by the 4.0 paradigm, but there are still cultural and technological limits to the full development of the phenomenon. This research aims to contribute to knowledge that will improve the propensity of agricultural operators to adopt the digital solutions of “Agriculture 4.0” by demonstrating its potential, along with its limits. To this end, an integrated methodological approach was adopted, built with focus groups and multicriteria analysis, to define and assess the possible future scenarios resulting from the implementation of digital transformation. The results show an increased focus on solutions that allow the integration of new tools to support those already used in the business organization and at a sustainable cost. To enable the development of “Agriculture 4.0”, we propose that it is necessary to invest in training operators in the supply chain, and above all, raising awareness among farmers, who it is essential fully appreciate the potential benefits of the 4.0 revolution.
Keywords	Citrus ; Internet ; agriculture ; blockchain ; business organization ; data analysis ; environmental sustainability ; markets ; supply chain ; Italy
Language	English
Dates of publication	2022-0312
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2651678-0
ISSN	2077-0472
ISSN	2077-0472
DOI	10.3390/agriculture12030400
Database	NAL-Catalogue (AGRICOLA)

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Article: The Evaluation of Green Investments in Urban Areas: A Proposal of an <i>eco-social-green</i> Model of the City

Sturiale, Luisa / Scuderi, Alessandro

Sustainability. 2018 Dec. 02, v. 10, no. 12

2018

Abstract: The new and more conscious sensibility towards the environmental sphere supports the idea of “green city”, promotes initiatives of structural integration of the green with the built environment and involves a considerable number of disciplines in a ... ...

Abstract	The new and more conscious sensibility towards the environmental sphere supports the idea of “green city”, promotes initiatives of structural integration of the green with the built environment and involves a considerable number of disciplines in a cultural and social debate. The literature reports different experiences of collaborative governance, between administrations and citizens, which tend to enhance the interaction between the different social actors involved in the investments of Green Infrastructures, to share objectives and management methods and to assess the extent of ecosystem services. The objective of this article is to propose a methodological approach to assessing green investments in the urban area, which is able to internalize the social perception of citizens regarding this important component for the urban landscape, with a view to guiding the city’s government towards a new urban eco-social-green planning and evaluation model. It presents a concise framework of the scientific debate on climate change and on the effects of urban planning issues; some relevant experiences of Green Infrastructures; and the proposed methodology, applied to the reality of the “urban green system” of Catania, based on an integrated approach between participatory planning and the method NAIADE (Novel Approach to Imprecise Assessment and Decision Environments).
Keywords	climate change ; ecosystem services ; governance ; green infrastructure ; landscapes ; models ; urban areas ; urban planning ; Italy
Language	English
Dates of publication	2018-1202
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2518383-7
ISSN	2071-1050
ISSN	2071-1050
DOI	10.3390/su10124541
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Higher frequency of TMEM199-CDG in the southern mediterranean area is associated with c.92G>C (p.Arg31Pro) mutation.

Fiumara, Agata / Sapuppo, Annamaria / Ferri, Lorenzo / Arena, Alessia / Prato, Adriana / Garozzo, Domenico / Sturiale, Luisa / Morrone, Amelia / Barone, Rita

European journal of medical genetics

2023 Volume 66, Issue 3, Page(s) 104709

Abstract: Congenital disorders of glycosylation (CDG) are genetic multisystem diseases, characterized by defective glycoconjugate synthesis. A small number of CDG with isolated liver damage have been described, such as TMEM199-CDG, a non-encephalopathic liver ... ...

Abstract	Congenital disorders of glycosylation (CDG) are genetic multisystem diseases, characterized by defective glycoconjugate synthesis. A small number of CDG with isolated liver damage have been described, such as TMEM199-CDG, a non-encephalopathic liver disorder with Wilson disease-like phenotype. Only eight patients with TMEM199-CDG have been described including seven Europeans (originating from Greece and Italy) and one Chinese. Three patients from southern Italy (Campania) shared the same known missense mutation pathogenetic variant NM_152464.3:c. 92G > C (p.Arg31Pro), also found in a Greek patient. Here we report a new patient from southern Italy (Sicily), with a homozygous c.92G > C p.(Arg31Pro) variant in TMEM199. The patient's phenotype is characterized by mild non-progressive hepatopathy with a normal hepatic echo structure. A persistent increase in serum transaminases, total and low-density lipoprotein cholesterol and low serum ceruloplasmin and copper levels and normal urinary copper excretion were observed. Matrix-assisted laser desorption/ionization mass spectrometry analyses showed abnormal N- and O- protein glycosylation, indicative of a Golgi processing defect and supporting the function of TMEM199 in maintaining Golgi homeostasis. TMEM199-CDG is an ultra-rare CDG relatively frequent in the southern Mediterranean area (7 in 9 patients, 77%). It is mainly associated with the c.92G > C (p.Arg31Pro) pathogenetic allele globally reported in 4 out of 7 families (57%), including one from Greece and three unrelated families from southern Italy. The almost uniform clinical phenotype described in patients with TMEM199-CDG appears to reflect a higher prevalence of the same variant in patients from the southern Mediterranean area.
MeSH term(s)	Humans ; Glycosylation ; Copper ; Mutation ; Hepatolenticular Degeneration ; Congenital Disorders of Glycosylation/pathology ; Membrane Proteins/genetics
Chemical Substances	Copper (789U1901C5) ; TMEM199 protein, human ; Membrane Proteins
Language	English
Publishing date	2023-01-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2184135-4
ISSN	1878-0849 ; 1769-7212
ISSN (online)	1878-0849
ISSN	1769-7212
DOI	10.1016/j.ejmg.2023.104709
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Article: The digital economy

Sturiale, Luisa / Scuderi, Alessandro

International journal of electronic marketing and retailing : IJEMR Vol. 7, No. 4 , p. 287-310

new e-business strategies for food Italian system

2016 Volume 7, Issue 4, Page(s) 287–310

Author's details	Luisa Sturiale, Alessandro Scuderi
Keywords	ICTs ; e-commerce ; web-consumer ; social media ; social commerce ; 8Ps marketing ; social network ; web-marketing ; social media marekting ; ICTs model ; electronic word of mouth ; e-WoM
Language	English
Publisher	Inderscience Enterprises Ltd.
Publishing place	Olney, Bucks.
Document type	Article
ZDB-ID	2193368-6
ISSN	1741-1025
Database	ECONomics Information System

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Article ; Online: N-Glycomics of Human Erythrocytes.

Bua, Rosaria Ornella / Messina, Angela / Sturiale, Luisa / Barone, Rita / Garozzo, Domenico / Palmigiano, Angelo

International journal of molecular sciences

2021 Volume 22, Issue 15

Abstract: Glycosylation is a complex post-translational modification that conveys functional diversity to glycoconjugates. Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen ... ...

Abstract	Glycosylation is a complex post-translational modification that conveys functional diversity to glycoconjugates. Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition. Red blood cell (RBC) membrane N-glycans determine blood type and influence cell lifespan. Although several proteomic studies have been carried out, the glycosylation of RBC membrane proteins has not been systematically investigated. This work aims at exploring the human RBC N-glycome by high-sensitivity MALDI-MS techniques to outline a fingerprint of RBC N-glycans. To this purpose, the MALDI-TOF spectra of healthy subjects harboring different blood groups were acquired. Results showed the predominant occurrence of neutral and sialylated complex N-glycans with bisected N-acetylglucosamine and core- and/or antennary fucosylation. In the higher mass region, these species presented with multiple N-acetyllactosamine repeating units. Amongst the detected glycoforms, the presence of glycans bearing ABO(H) antigens allowed us to define a distinctive spectrum for each blood group. For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.
MeSH term(s)	Blood Group Antigens/metabolism ; Erythrocytes/metabolism ; Glycomics ; Glycosylation ; Humans ; Polysaccharides/analysis ; Protein Processing, Post-Translational ; Proteomics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical Substances	Blood Group Antigens ; Polysaccharides
Language	English
Publishing date	2021-07-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22158063
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Article ; Online: Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.

Papazoglu, Gabriela Magali / Cubilla, Marisa / Pereyra, Marcela / de Kremer, Raquel Dodelson / Pérez, Belén / Sturiale, Luisa / Asteggiano, Carla Gabriela

Glycoconjugate journal

2021 Volume 38, Issue 2, Page(s) 191–200

Abstract: Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum. Variants in ALG2cause a congenital disorder of glycosylation (CDG) known as ALG2-CDG. Up to date, nine ALG2-CDG ... ...

Abstract	Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum. Variants in ALG2cause a congenital disorder of glycosylation (CDG) known as ALG2-CDG. Up to date, nine ALG2-CDG patients have been reported worldwide. ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression. In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing. Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans. Moreover, we have observed a slight oligomannose accumulation in total serum glyco-profiles. The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG. Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis. This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.
MeSH term(s)	Argentina ; Child ; Child, Preschool ; Congenital Disorders of Glycosylation/etiology ; Congenital Disorders of Glycosylation/genetics ; Female ; Glycosylation ; Homozygote ; Humans ; Isoelectric Focusing ; Male ; Mannosyltransferases/genetics ; Phenotype ; Polysaccharides/analysis ; Polysaccharides/blood ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Transferrin/metabolism ; Whole Exome Sequencing
Chemical Substances	Polysaccharides ; Transferrin ; Mannosyltransferases (EC 2.4.1.-)
Language	English
Publishing date	2021-03-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	283770-5
ISSN	1573-4986 ; 0282-0080
ISSN (online)	1573-4986
ISSN	0282-0080
DOI	10.1007/s10719-021-09976-w
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Zs.A 2171: Show issues

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Article ; Online: Tear N-glycomics in vernal and atopic keratoconjunctivitis.

Messina, Angela / Palmigiano, Angelo / Tosto, Claudia / Romeo, Donata Agata / Sturiale, Luisa / Garozzo, Domenico / Leonardi, Andrea

Allergy

2021 Volume 76, Issue 8, Page(s) 2500–2509

Abstract: Purpose: Tear fluid N-Glycome from patients affected with vernal (VKC) and atopic keratoconjunctivitis (AKC) was investigated to identify specific changes in tears and to recognize possible glyco-biomarkers.: Methods: The analysis of the N-glycans ... ...

Abstract	Purpose: Tear fluid N-Glycome from patients affected with vernal (VKC) and atopic keratoconjunctivitis (AKC) was investigated to identify specific changes in tears and to recognize possible glyco-biomarkers. Methods: The analysis of the N-glycans was performed using matrix-assisted laser desorption ionization mass spectrometry on single tear samples. Tears from control normal subjects (CTRL), VKC and AKC patients were processed and treated with peptide N-glycosidase F (PNGase F) to deglycosylate N-glycoproteins. Released N-glycans were purified, permethylated, and analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and tandem mass spectrometry (MALDI-TOF MS and MALDI-TOF MS/MS). Results: More than 150 complex N-glycans, including highly fucosylated biantennary, triantennary, tetra-antennary, and bisecting species, were observed in our spectra. Three distinct patterns for CTRL, VKC, and AKC patients were identified in terms of relative intensities for some N-glycans structures. Major variations involved bisecting and hyperfucosylated glycoforms. The most intense ions were associated with species at m/z 1907.0 (asialo, agalacto, bisected, biantennary structure-NGA2B) in CTRL MS profiles, at m/z 2605.3 and 2966.5 in VKC, and at m/z 2792.4 in AKC corresponding to a well-known biantennary, disialylated N-glycan. Several peaks were associated with structures bearing one or two Lewis X epitopes. Structures were confirmed by MS/MS analysis. Quantitative differences among the three groups were statistically significant. Conclusions: Tear MS profiles are rich in specific glycoforms, particularly those with a high fucosylation degree, indicating both core and peripheral decoration. Tear N-glycome analysis provided important information for a better comprehension of VKC and AKC alterations at the molecular level.
MeSH term(s)	Conjunctivitis, Allergic/diagnosis ; Glycomics ; Humans ; Keratoconjunctivitis ; Polysaccharides ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Tandem Mass Spectrometry ; Tears
Chemical Substances	Polysaccharides
Language	English
Publishing date	2021-03-15
Publishing country	Denmark
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	391933-x
ISSN	1398-9995 ; 0105-4538
ISSN (online)	1398-9995
ISSN	0105-4538
DOI	10.1111/all.14775
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Article: Secondary deficiency of neuraminidase 1 contributes to CNS pathology in neurological mucopolysaccharidoses via hypersialylation of brain glycoproteins.

bioRxiv : the preprint server for biology

2024

Language	English
Publishing date	2024-04-27
Publishing country	United States
Document type	Preprint
DOI	10.1101/2024.04.26.587986
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