LIVIVO - Search results -

Search results

Result 1 - 10 of total 50

Search options

Article ; Online: The failure behavior of coal-rock combined body under compression-shear loading.

Li, Xiaopeng / Li, Xiangshang / Yuan, Honghui / Li, Haitao / Yang, Guanyu / Wang, Shuwen / Ding, Guoli / Su, Shijie

2024 Volume 107, Issue 1, Page(s) 368504231225860

Abstract: As shallow coal reserves continue to deplete rapidly, deep mining has become an unavoidable course of action. In the process of deep coal mining, affected by blasting, mining, and excavation, the coal-rock interface often encounters the action of ... ...

Abstract	As shallow coal reserves continue to deplete rapidly, deep mining has become an unavoidable course of action. In the process of deep coal mining, affected by blasting, mining, and excavation, the coal-rock interface often encounters the action of compression-shear composite load. The interface crack directly affects the stability of the coal-rock structure. Uniaxial compression experiments have been conducted on rock and coal-like material with pre-existing interfacial oblique cracks to study the crack propagation criterion of the rock and coal-like material interface. An image acquisition system is used to record the surface speckle field of the whole process of specimen failure. The strain fields and stress intensity factor of the sample at different times are obtained by digital image correlation. At the same time, the stress field near the crack tip is calculated. The results show that with the increase of loading, two strains of localization bands are formed on the surface of the specimen. One of the strain-localized bands starts at the lower tip of the prefabricated crack and propagates along the vertical interface. The other starts at the upper tip of the prefabricated damage and propagates along the interface direction. It can be seen that the lower tip of the prefabricated crack enters into the crack fracture process zone earlier than the upper tip. The strain localization band narrows gradually with the load increase, and then macro cracks appear. The initiation of two tips is suitable for different fracture criteria. The lower tip is dominated by the maximum circumferential tensile stress, and the upper tip is dominated by shear stress. The specimens eventually fracture along the interface. The evolution of the strain field during the failure process of rock and coal-like material can reflect the generation and propagation of the crack.
Language	English
Publishing date	2024-01-10
Publishing country	England
Document type	Journal Article
ZDB-ID	128412-5
ISSN	2047-7163 ; 0036-8504 ; 0302-1785
ISSN (online)	2047-7163
ISSN	0036-8504 ; 0302-1785
DOI	10.1177/00368504231225860
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 731: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Effects of sleep deprivation on anxiety-depressive-like behavior and neuroinflammation.

Cao, Dandan / Zhao, Yi / Wang, Yuting / Wei, Dongyun / Yan, Minhao / Su, Shijie / Pan, Huashan / Wang, Qi

Brain research

2024 Volume 1836, Page(s) 148916

Abstract: Background: Depression is defined by a persistent low mood and disruptions in sleep patterns, with the WHO forecasting that major depression will rank as the third most prevalent contributor to the global burden of disease by the year 2030. Sleep ... ...

Abstract	Background: Depression is defined by a persistent low mood and disruptions in sleep patterns, with the WHO forecasting that major depression will rank as the third most prevalent contributor to the global burden of disease by the year 2030. Sleep deprivation serves as a stressor that triggers inflammation within the central nervous system, a process known as neuroinflammation. This inflammatory response plays a crucial role in the development of depression by upregulating the expression of inflammatory mediators that contribute to symptoms such as anxiety, hopelessness, and loss of pleasure. Methods: In this study, sleep deprivation was utilized as a method to induce anxiety and depressive-like behaviors in mice. The behavioral changes in the mice were then evaluated using the EZM, EPM, TST, FST, and SPT. H&E staining and Nissl staining was used to detect morphological changes in the medial prefrontal cortical (mPFC) regions. Elisa to assess serum CORT levels. Detection of mRNA levels and protein expression of clock genes, high mobility genome box-1 (Hmgb1), silent message regulator 6 (Sirt6), and pro-inflammatory factors by RT-qPCR, Western blotting, and immunofluorescence techniques. Results: Sleep deprivation resulted in decreased exploration of unfamiliar territory, increased time spent in a state of despair, and lower sucrose water intake in mice. Additionally, sleep deprivation led to increased secretion of serum CORT and upregulation of clock genes, IL6, IL1β, TNFα, Cox-2, iNOS, Sirt6, and Hmgb1. Sleep. Conclusions: Sleep deprivation induces anxiety-depressive-like behaviors and neuroinflammation in the brain. Transcription of clock genes and activation of the Sirt6/Hmgb1 pathway may contribute to inflammatory responses in the mPFC.
Language	English
Publishing date	2024-04-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1200-2
ISSN	1872-6240 ; 0006-8993
ISSN (online)	1872-6240
ISSN	0006-8993
DOI	10.1016/j.brainres.2024.148916
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 161: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: iTRAQ-based proteomic analysis reveals invasion-related proteins among three developmental stages of Eimeria necatrix

Gao, Yang / Suding, Zeyang / Wang, Lele / Liu, Dandan / Su, Shijie / Xu, Jinjun / Hu, Junjie / Tao, Jianping

Journal of Proteomics. 2023 May 25, p.104939-

2023 , Page(s) 104939–

Abstract: Eimeria necatrix is an obligate intracellular parasite that has a complex life cycle and causes significant economic losses to the poultry industry. To better understand the cellular invasion mechanism of E. necatrix and develop new measures against its ... ...

Abstract	Eimeria necatrix is an obligate intracellular parasite that has a complex life cycle and causes significant economic losses to the poultry industry. To better understand the cellular invasion mechanism of E. necatrix and develop new measures against its infection, we conducted isobaric tags for relative and absolute quantitation (iTRAQ) proteomic analysis to investigate protein abundance across different life cycle stages, including unsporulated oocysts (UO), sporozoites (SZ) and second-generation merozoites (MZ-2). Our analysis identified a total of 3606 proteins, among which 1725, 1724, 2143 and 2386 were annotated by the Gene Ontology (GO), EuKaryotic Orthologous Groups (KOG), Kyoto Encyclopedia of Genes and Genomes (KEGG) and InterPro (IPR) databases, respectively. We also identified 388, 300 and 592 differentially abundant proteins in SZ vs UO, SZ vs MZ-2 and MZ-2 vs UO, respectively. Further analysis revealed that 118 differentially abundant proteins were involved in cellular invasion and could be categorized into eight groups. These findings provide valuable insights into protein abundance across the different life cycle stages of E. necatrix and offer candidate proteins for future studies on cellular invasion and other biological processes. Eimeria necatrix is an obligate intracellular parasite results in huge economic losses to the poultry industry. Understanding proteomic variations across the life cycle stages of E. necatrix may offer proteins related to cellular invasion of E. necatrix, and provide resources for the development of new treatment and prevention interventions against E. necatrix infection. The current data provide an overall summary of the protein abundance across the three life cycle stages of E. necatrix. We identified differentially abundant proteins potential related to cellular invasion. The candidate proteins we identified will form the basis of future studies for cellular invasion. This work also will help in the development of novel strategies for coccidiosis control.
Keywords	Eimeria necatrix ; coccidiosis ; gene ontology ; genome ; merozoites ; oocysts ; parasites ; poultry industry ; proteomics ; sporozoites ; Chicken ; Life cycle ; Differentially abundant proteins ; Functional annotation ; Cellular invasion
Language	English
Dates of publication	2023-0525
Publishing place	Elsevier B.V.
Document type	Article ; Online
Note	Pre-press version
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2023.104939
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Kai-Xin-San protects against mitochondrial dysfunction in Alzheimer's disease through SIRT3/NLRP3 pathway.

Su, ShiJie / Chen, Gongcan / Gao, Minghuang / Zhong, Guangcheng / Zhang, Zerong / Wei, Dongyun / Luo, Xue / Wang, Qi

Chinese medicine

2023 Volume 18, Issue 1, Page(s) 26

Abstract: Background: Kai-Xin-San (KXS) has been reported to have a good curative impact on dementia. The purpose of the study was to determine whether KXS might ameliorate cognitive deficits in APP/PS1 mice and to evaluate its neuroprotective mechanism.: ... ...

Abstract	Background: Kai-Xin-San (KXS) has been reported to have a good curative impact on dementia. The purpose of the study was to determine whether KXS might ameliorate cognitive deficits in APP/PS1 mice and to evaluate its neuroprotective mechanism. Methods: APP/PS1 mice were employed as an AD animal model; Aβ Results: The results indicated that KXS protected APP/PS1 mice against cognitive impairments. KXS suppressed neuronal apoptosis and oxidative stress among APP/PS1 mice. KXS and KXS-containing serum improved mitochondrial dysfunction and synaptic and neurotrophic factors regarding APP/PS1 mice. In addition, KXS and KXS-containing serum enhanced mitochondrial SIRT3 expression and reduced NLRP3 inflammasome expression in APP/PS1 mice. Conclusion: KXS improves cognitive dysfunction among APP/PS1 mice via regulating SIRT3-mediated neuronal cell apoptosis. These results suggested that KXS was proposed as a neuroprotective agent for AD progression.
Language	English
Publishing date	2023-03-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2260322-0
ISSN	1749-8546
ISSN	1749-8546
DOI	10.1186/s13020-023-00722-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Localization

Cai, Weimin / Feng, Qianqian / Wang, Liyue / Su, Shijie / Hou, Zhaofeng / Liu, Dandan / Kang, Xilong / Xu, Jinjun / Pan, Zhiming / Tao, Jianping

Frontiers in cellular and infection microbiology

2023 Volume 13, Page(s) 1305727

Abstract: Introduction: Apicomplexan AP2 family of proteins (ApiAP2) are transcription factors (TFs) that regulate parasite growth and development, but little is known about the ApiAP2 TFs in : Methods: The cDNAs encoding full-length and truncated EnApiAP2 ... ...

Abstract	Introduction: Apicomplexan AP2 family of proteins (ApiAP2) are transcription factors (TFs) that regulate parasite growth and development, but little is known about the ApiAP2 TFs in Methods: The cDNAs encoding full-length and truncated EnApiAP2 protein were cloned and sequenced, respectively. Then, the two cDNAs were cloned into the pET28a(+) expression vector and expressed expressed in Results: The En Discussion: EnApiAP2 protein encoded by
MeSH term(s)	Animals ; Adaptor Proteins, Signal Transducing/metabolism ; Chickens/genetics ; DNA, Complementary/genetics ; Eimeria/genetics ; Eimeria/metabolism ; Microfilament Proteins/metabolism ; Nuclear Proteins/metabolism ; Poultry Diseases/parasitology ; Sporozoites/metabolism
Chemical Substances	Adaptor Proteins, Signal Transducing ; DNA, Complementary ; ENH protein, mouse ; Microfilament Proteins ; Nuclear Proteins
Language	English
Publishing date	2023-12-05
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2023.1305727
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Characterization of the novel glucose-methanol-choline (GMC) oxidoreductase EnOXIO1 in Eimeria necatrix.

Wang, Feiyan / Ye, Zhuang / Liu, Yue / Wang, Lele / Su, Shijie / Hou, Zhaofeng / Xu, Jinjun / Tao, Jianping / Liu, Dandan

Veterinary parasitology

2023 Volume 321, Page(s) 110002

Abstract: Eimeria species are intracellular obligate parasites, among the most common pathogens affecting the intensive poultry industry. Oxidoreductases are members of a class of proteins with redox activity and are widely found in apicomplexan protozoans. ... ...

Abstract	Eimeria species are intracellular obligate parasites, among the most common pathogens affecting the intensive poultry industry. Oxidoreductases are members of a class of proteins with redox activity and are widely found in apicomplexan protozoans. However, there have been few reports related to Eimeria species. In this study, total RNA was extracted from the gametocytes of E. necatrix Yangzhou strain to amplify the EnOXIO1 gene using reverse-transcription polymerase chain reaction. After cloning and sequence analysis, the prokaryotic expression vector pET-28a(+)-EnOXIO1 was constructed and transformed into Escherichia coli BL21(DE3), and the recombinant protein rEnOXIO1 was expressed by induction with isopropyl ß-D-1-thiogalactopyranoside. The full length EnOXIO1 gene was 2535 bp encoding 844 amino acids, and the EnOXIO1 protein had a molecular weight of about 100 kDa and was mainly expressed in inclusion bodies. Western blot analysis indicated that the rEnOXIO1 protein had good antigenicity and cross-reactivity and was specifically recognized by a 6 ×HIS labeled monoclonal antibody, mouse anti-recombinant protein polyclonal antibody, and recovery serum from chickens infected with E. necatrix, E. acervulina, and E. tenella sporulated oocysts. The results of laser confocal immunofluorescence localization showed that the EnOXIO1 protein was mainly located on the wall-forming bodies in gametocytes and played an important role in the formation of the oocyst wall. Quantitative PCR analysis revealed that transcript levels of EnOXIO1 were highest in the gametocyte stage. Protein expression levels of EnOXIO1 were higher in the gametocyte stage than in other developmental stages according to western blot analysis. Vaccination of chickens against E. necatrix was achieved with recombinant protein rEnOXIO1, which triggered humoral immunity and antibody production, increased average body weight gain, reduced oocyst output and alleviated lesions after E. necatrix infection. The highest ACI value (172.36) was observed in chickens that received 200 μg rEnOXIO1 compared with other immunization groups.
MeSH term(s)	Animals ; Mice ; Eimeria/genetics ; Methanol/metabolism ; Coccidiosis/parasitology ; Coccidiosis/veterinary ; Protozoan Proteins/genetics ; Chickens/parasitology ; Recombinant Proteins ; Oocysts ; Oxidoreductases ; Glucose/metabolism ; Eimeria tenella ; Poultry Diseases/parasitology
Chemical Substances	Methanol (Y4S76JWI15) ; Protozoan Proteins ; Recombinant Proteins ; Oxidoreductases (EC 1.-) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2023-08-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	196831-2
ISSN	1873-2550 ; 0304-4017
ISSN (online)	1873-2550
ISSN	0304-4017
DOI	10.1016/j.vetpar.2023.110002
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4380: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Hesperetin attenuates cognitive dysfunction via SIRT6/NLRP3 pathway in scopolamine-induced mice.

Jing, Shangwen / Wang, Xinyue / Zhang, Zerong / Cao, Dandan / Huang, Kongli / Wang, Yuting / Liu, Zhuangzhuang / Su, Shijie / Wang, Qi

Metabolic brain disease

2023 Volume 38, Issue 7, Page(s) 2443–2456

Abstract: Neuroinflammation is a critical feature in the pathogenesis of neurodegenerative disorders such as Alzheimer's disease (AD). Hesperetin can exert anti-inflammatory, antioxidant and other neuroprotective effects. In this study, the scopolamine (SCOP)- ... ...

Abstract	Neuroinflammation is a critical feature in the pathogenesis of neurodegenerative disorders such as Alzheimer's disease (AD). Hesperetin can exert anti-inflammatory, antioxidant and other neuroprotective effects. In this study, the scopolamine (SCOP)-induced cognitive dysfunction in mice model was used to evaluate the neuroprotective effects of hesperetin. Behavioral tests (Morris water maze, open field, and novel object recognition tests) were conducted to evaluate the effect of hesperetin on cognitive dysfunction behaviors. Nissl staining and Immunofluorescence were used to evaluate hippocampal neuronal damage and microglial activation in mice. The levels of proinflammatory factors, oxidant stress, and the cholinergic neurotransmitter were detected by real-time quantitative fluorescence PCR (RT-qPCR) or biochemical reagent kits. Western blotting was used to detect the relative protein expression of the sirtuin 6 (SIRT6) / NOD-like receptor thermal protein domain associated protein 3 (NLRP3) pathway. Results showed that hesperetin could ameliorate SCOP-induced cognitive impairment and neuronal damage, and regulate the levels of cholinergic neurotransmitters in the hippocampal of AD mice. Hesperetin could also enhance antioxidant defense by regulating the levels of reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT). Hesperetin exerted anti-neuroinflammation effects through inhibiting of microglia activation and down-regulating the mRNA transcript levels of inflammatory cytokines, such as tumor necrosis factor α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS). Meanwhile, hesperetin could attenuate the expression of NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC), thioredoxin-interacting protein (TXNIP), and caspase-1 p20 and upregulate the expression of SIRT6 in SCOP-induced mice. Overall, our study suggested that hesperetin might ameliorate SCOP-induced cognitive dysfunction by improving cholinergic system dysfunction and suppressing oxidative stress and attenuating neuroinflammation via SIRT6/NLRP3 pathway in mice.
MeSH term(s)	Mice ; Animals ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Inflammasomes/metabolism ; Antioxidants ; Neuroprotective Agents/pharmacology ; Neuroprotective Agents/therapeutic use ; Scopolamine ; Cognitive Dysfunction/chemically induced ; Cognitive Dysfunction/drug therapy ; Sirtuins
Chemical Substances	NLR Family, Pyrin Domain-Containing 3 Protein ; hesperetin (Q9Q3D557F1) ; Inflammasomes ; Antioxidants ; Neuroprotective Agents ; Scopolamine (DL48G20X8X) ; Sirtuins (EC 3.5.1.-)
Language	English
Publishing date	2023-06-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	632824-6
ISSN	1573-7365 ; 0885-7490
ISSN (online)	1573-7365
ISSN	0885-7490
DOI	10.1007/s11011-023-01250-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2155: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: iTRAQ-based proteomic analysis reveals invasion-related proteins among three developmental stages of Eimeria necatrix.

Gao, Yang / Suding, Zeyang / Wang, Lele / Liu, Dandan / Su, Shijie / Xu, Jinjun / Hu, Junjie / Tao, Jianping

Journal of proteomics

2023 Volume 283-284, Page(s) 104939

Abstract	Eimeria necatrix is an obligate intracellular parasite that has a complex life cycle and causes significant economic losses to the poultry industry. To better understand the cellular invasion mechanism of E. necatrix and develop new measures against its infection, we conducted isobaric tags for relative and absolute quantitation (iTRAQ) proteomic analysis to investigate protein abundance across different life cycle stages, including unsporulated oocysts (UO), sporozoites (SZ) and second-generation merozoites (MZ-2). Our analysis identified a total of 3606 proteins, among which 1725, 1724, 2143 and 2386 were annotated by the Gene Ontology (GO), EuKaryotic Orthologous Groups (KOG), Kyoto Encyclopedia of Genes and Genomes (KEGG) and InterPro (IPR) databases, respectively. We also identified 388, 300 and 592 differentially abundant proteins in SZ vs UO, SZ vs MZ-2 and MZ-2 vs UO, respectively. Further analysis revealed that 118 differentially abundant proteins were involved in cellular invasion and could be categorized into eight groups. These findings provide valuable insights into protein abundance across the different life cycle stages of E. necatrix and offer candidate proteins for future studies on cellular invasion and other biological processes. SIGNIFICANCE: Eimeria necatrix is an obligate intracellular parasite results in huge economic losses to the poultry industry. Understanding proteomic variations across the life cycle stages of E. necatrix may offer proteins related to cellular invasion of E. necatrix, and provide resources for the development of new treatment and prevention interventions against E. necatrix infection. The current data provide an overall summary of the protein abundance across the three life cycle stages of E. necatrix. We identified differentially abundant proteins potential related to cellular invasion. The candidate proteins we identified will form the basis of future studies for cellular invasion. This work also will help in the development of novel strategies for coccidiosis control.
MeSH term(s)	Animals ; Eimeria/genetics ; Eimeria/metabolism ; Proteomics ; Coccidiosis ; Oocysts/genetics ; Life Cycle Stages ; Poultry ; Chickens
Language	English
Publishing date	2023-05-25
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2023.104939
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Network Proximity-based computational pipeline identifies drug candidates for different pathological stages of Alzheimer's disease

Wu, Qihui / Su, Shijie / Cai, Chuipu / Xu, Lina / Fan, Xiude / Ke, Hanzhong / Dai, Zhao / Fang, Shuhuan / Zhuo, Yue / Wang, Qi / Pan, Huafeng / Gu, Yong / Fang, Jiansong

Computational and Structural Biotechnology Journal. 2023, v. 21 p.1907-1920

2023

Abstract: Despite the massive investment in Alzheimer’s disease (AD), there are still no disease-modifying treatments (DMTs) for AD. One major reason is attributed to the limitation of clinical "one‐size‐fits‐all” approach, since the same AD treatment solely based ...

Abstract	Despite the massive investment in Alzheimer’s disease (AD), there are still no disease-modifying treatments (DMTs) for AD. One major reason is attributed to the limitation of clinical "one‐size‐fits‐all” approach, since the same AD treatment solely based on clinical diagnosis was unlikely to achieve good clinical efficacy. In recent years, computational approaches based on multiomics data have provided an unprecedented opportunity for drug discovery since they can substantially lower the costs and boost the efficiency. In this study, we intended to identify potential drug candidates for different pathological stages of AD by computationally repurposing Food and Drug Administration (FDA) approved drugs. First, we assembled gene expression data from three different AD pathological stages, which include mild cognitive impairment (MCI) and early and late stages of AD (EAD, LAD). We next quantified the network distances between drug target networks and AD modules by utilizing a network proximity approach, and identified 193 candidates that possessed significant associations with AD. After searching for previous literature evidence, 63 out of 193 (32.6%) predicted drugs were demonstrated to exert therapeutic effects on AD. We further explored the novel mechanism of action (MOA) for these drug candidates by determining the specific brain cells they might function on based on AD patient single cell transcriptomic data. Additionally, we selected several promising candidates that could cross the blood brain barrier together with confirmed neuroprotective effects, and subsequently determined the antioxidative activity of these compounds. Experimental results showed that azathioprine decreased the reactive oxygen species (ROS) and malondialdehyde (MDA) levels and improved the superoxide dismutase (SOD) activity in APP-SH-SY5Y cells. Finally, we deciphered the potential MOA of azathioprine against AD via network analysis and validated several apoptosis-related proteins (Caspase 3, Cleaved Caspase 3, Bax, Bcl2) through western blotting. In summary, this study presented an effective computational strategy utilizing omics data for AD drug repurposing, which provides a new perspective for drug discovery and development.
Keywords	Alzheimer disease ; Food and Drug Administration ; antioxidant activity ; biotechnology ; blood-brain barrier ; brain ; caspase-3 ; cognitive disorders ; drugs ; gene expression ; malondialdehyde ; mechanism of action ; multiomics ; patients ; reactive oxygen species ; superoxide dismutase ; therapeutics ; transcriptomics ; Drug repurposing ; Network proximity ; Alzheimer’s disease ; Omics ; in vitro experimental validation
Language	English
Size	p. 1907-1920.
Publishing place	Elsevier B.V.
Document type	Article ; Online
Note	Use and reproduction
ZDB-ID	2694435-2
ISSN	2001-0370
ISSN	2001-0370
DOI	10.1016/j.csbj.2023.02.041
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Preparation of aligned nanofibers using parallel inductive-plates assisted electrospinning.

Su, Shijie / Liang, Junsheng / Xu, Shuangchao / Li, Xiaojian / Xin, Wenwen / Wang, Zizhu / Wang, Dazhi

Nanotechnology

2021 Volume 32, Issue 26

Abstract: Electrospinning is a simple, cost-effective, and versatile technique for fabrication of nanofibers. However, nanofibers obtained from the conventional electrospinning are typically disordered, which seriously limits their application. In this work, we ... ...

Abstract	Electrospinning is a simple, cost-effective, and versatile technique for fabrication of nanofibers. However, nanofibers obtained from the conventional electrospinning are typically disordered, which seriously limits their application. In this work, we present a novel and facile technique to obtain aligned nanofibers with high efficiency by using parallel inductive-plates assisted electrospinning (PIES). In this new electrospinning setup, the electrostatic spinneret is contained in a pair of parallel inductive-plates, which can change the shape and direction of the electric field line during the electrospinning so as to control the flight trajectory and spatial alignment of the spinning nanofibers. This electrospinning setup can divide the electric field line into two parts which are respectively directed to the edge of the upper and lower inductive-plates. Then the nanofibers move along the electric field line, suspend and align between the parallel inductive-plates. Finally, the well aligned nanofibers could be easily transferred onto other substrates for further characterizations and applications. The aligned nanofibers with an average diameter of 469 ± 115 nm and a length as long as 140 mm were successfully achieved by using PIES technique. Moreover, nanofiber arrays with different cross angles and three-dimensional films formed by the aligned nanofibers were also facilely obtained. The novel PIES developed in this work has been proved to be a facile, cost-effective and promising approach to prepare aligned nanofibers for a wide range of applications.
Language	English
Publishing date	2021-04-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1362365-5
ISSN	1361-6528 ; 0957-4484
ISSN (online)	1361-6528
ISSN	0957-4484
DOI	10.1088/1361-6528/abf073
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito