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Article: [Construction of yeast cell factories for production of azadirachtin precursor tirucalla-7,24-dien-3β-ol].

Liu, Jia-Rou / Su, Xin-Yao / Liu, Jin-Long / Wang, Cai-Xia

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2021 Volume 46, Issue 19, Page(s) 4959–4968

Abstract: Azadirachtin, as a botanical insecticide, is a highly oxidized limonoid triterpenoid existing in the seeds of Azadirachta indica. However, due to the low content in the seeds, the production of azadirachtin by seed extraction has low yield. Chemical ... ...

Abstract	Azadirachtin, as a botanical insecticide, is a highly oxidized limonoid triterpenoid existing in the seeds of Azadirachta indica. However, due to the low content in the seeds, the production of azadirachtin by seed extraction has low yield. Chemical synthesis of azadirachtin is characterized by complex process and low yield. Synthetic biology provides an alternative for the supply of azadirach-tin. In this study, two oxidosqualene cyclases AiOSC1 and MaOSC1 respectively derived from A. indica and Melia azedarach were identified in yeast. A yeast strain producing tirucalla-7,24-dien-3β-ol was constructed by integration of AiOSC1, Arabidopsis thaliana-derived squalene synthase gene(AtAQS2), and Saccharomyces cerevisiae-derived truncated 3-hydroxy-3-methyl-glutaryl coenzyme A reductase gene(PgtHMGR) into the delta site of yeast. Then, the function of MaCYP71BQ5 was successfully verified in yeast after this gene was introduced into the constructed yeast strain. This study not only laid a foundation for the biosynthesis of tirucalla-7,24-dien-3β-ol, but also provided a chassis cell for the functional identification of cytochrome oxidases(CYP450 s) in azadirachtin biosynthesis pathway.
MeSH term(s)	Azadirachta ; Limonins ; Saccharomyces cerevisiae/genetics ; Triterpenes
Chemical Substances	Limonins ; Triterpenes ; azadirachtin (O4U1SAF85H)
Language	Chinese
Publishing date	2021-11-04
Publishing country	China
Document type	Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.19540/j.cnki.cjcmm.20210720.101
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Pathogenicity and drug resistance of the Eimeria tenella isolate from Yiwu, Zhejiang province, eastern China.

Sun, Hongchao / Su, Xinyao / Fu, Yuan / Hao, Lili / Zhou, Wei / Zhou, Zhijin / Huang, Jing / Wang, Yimin / Shi, Tuanyuan

Poultry science

2023 Volume 102, Issue 8, Page(s) 102845

Abstract: Chicken coccidiosis can cause severe enteritis with high mortality, which causes serious economic losses to the global breeding industry each year. The most virulent species is Eimeria tenella (E. tenella), but the infectivity of different E. tenella ... ...

Abstract	Chicken coccidiosis can cause severe enteritis with high mortality, which causes serious economic losses to the global breeding industry each year. The most virulent species is Eimeria tenella (E. tenella), but the infectivity of different E. tenella varies among geographic strains. At present, there are no reports related to the pathogenicity and drug resistance of E. tenella in Yiwu, Zhejiang province, China. A total of 600 fecal samples were collected from 10 farms in Zhejiang province, the overall oocyst prevalence was 54.2% (325/600). The prevalence was significantly higher (P < 0.01) in chickens under 40 d (97.5%) than that in chickens between 60 and 85-days-old (40.5%) and chickens over 90-days-old (24.5%). E. tenella stain was isolated from fecal samples of chickens in Yiwu and the pathogenicity of this isolate was determined, and then we recorded the survival rate, bloody stool score, lesion score, average weight gain. The results showed that all of the chickens infected with 5 × 10
MeSH term(s)	Animals ; Eimeria tenella ; Clopidol ; Nicarbazin ; Virulence ; Chickens ; Coccidiosis/epidemiology ; Coccidiosis/veterinary ; Weight Gain ; Drug Resistance ; Poultry Diseases/epidemiology
Chemical Substances	sulfachlorpyrazine (96R84UZ2XA) ; Clopidol (8J763HFF5N) ; Nicarbazin (11P9NUA12U)
Language	English
Publishing date	2023-06-05
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2023.102845
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Article ; Online: Production of 11-Oxo-β-Amyrin in

Sun, Mengchu / Xin, Qi / Hou, Kangxin / Qiu, Jie / Wang, Linmei / Chao, Erkun / Su, Xinyao / Zhang, Xiuxin / Chen, Shilin / Wang, Caixia

Journal of agricultural and food chemistry

2023 Volume 71, Issue 8, Page(s) 3766–3776

Abstract: The production of glycyrrhetinic acid (GA) and 11-oxo-β-amyrin, the major bioactive components in liquorice, was typically inhibited by P450 oxidation ... ...

Abstract	The production of glycyrrhetinic acid (GA) and 11-oxo-β-amyrin, the major bioactive components in liquorice, was typically inhibited by P450 oxidation in
MeSH term(s)	Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Cytochrome P-450 Enzyme System/genetics ; Cytochrome P-450 Enzyme System/metabolism ; Oleanolic Acid ; Oxidation-Reduction
Chemical Substances	beta-amyrin (KM8353IPSO) ; Cytochrome P-450 Enzyme System (9035-51-2) ; Oleanolic Acid (6SMK8R7TGJ)
Language	English
Publishing date	2023-02-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.2c08261
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Article: [Cloning and expression analysis of UDP-rhamnose synthase from Citrus sinensis].

Yu, Wan-Tong / Hou, Kang-Xin / Su, Xin-Yao / Zhang, Xiao-Li / Qiu, Jie / Wang, Cai-Xia / Xue, Qiang / Liu, Jin-Long

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2022 Volume 47, Issue 12, Page(s) 3208–3214

Abstract: Uridine diphosphate rhamnose(UDP-Rha), a glycoside donor synthesized with the catalysis of rhamnose synthase(RHM), is one of the important elements in the synthesis of rhamnosides. In this study, we cloned a RHM gene from Citrus sinensis(CsRHM) and ... ...

Abstract	Uridine diphosphate rhamnose(UDP-Rha), a glycoside donor synthesized with the catalysis of rhamnose synthase(RHM), is one of the important elements in the synthesis of rhamnosides. In this study, we cloned a RHM gene from Citrus sinensis(CsRHM) and analyzed its bioinformatic information and functions in vitro. The results showed the gene consisted of an open reading frame of 2 007 bp encoding 668 amino acid residues. The deduced protein had a presumed molecular weight of 75.27 kDa, a theoretical isoelectric point of 6.97, and the characteristic signal sequences(GxxxGxxG/A and YxxxK) of the RHM family. Multiple sequence alignments and the phylogenetic tree demonstrated that CsRHM shared homology with other RHMs. The results of enzymatic reactions in vitro showed that the recombinant protein CsRHM catalyzed the conversion of UDP-Glu to UDP-Rha, with the kinetic parameters V_(max), K_m, K_(cat), and K_(cat)/K_m of 0.373 7 μmol·L~(-1)·min~(-1), 21.29 μmol·L~(-1), 0.24 s~(-1), and 1.13×10~4 s~(-1)·L·mol~(-1), respectively. This study is the first report about CsRHM with validated catalytic function in vitro, which provides a foundation for further research on the biosynthesis of UDP-Rha.
MeSH term(s)	Citrus sinensis/genetics ; Citrus sinensis/metabolism ; Cloning, Molecular ; Phylogeny ; Rhamnose/chemistry ; Rhamnose/metabolism ; Uridine Diphosphate Sugars
Chemical Substances	Uridine Diphosphate Sugars ; UDP-rhamnose (1955-26-6) ; Rhamnose (QN34XC755A)
Language	Chinese
Publishing date	2022-07-18
Publishing country	China
Document type	Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.19540/j.cnki.cjcmm.20220216.101
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Article ; Online: Metabolic Engineering of

Hou, Kangxin / Yu, Wantong / Wang, Xiaojiao / Liu, Jiarou / Liu, Yan / Liu, Jia / Su, Xinyao / Zhang, Xiaoli / Xue, Qiang / Wang, Caixia

Journal of agricultural and food chemistry

2022 Volume 70, Issue 11, Page(s) 3467–3476

Abstract: Azadirachtin, a limonoid isolated from the neem tree, has attracted considerable interest due to its excellent performance in pest control. Studies have also reported pharmaceutical activities of dihydroniloticin, an intermediate in azadirachtin ... ...

Abstract	Azadirachtin, a limonoid isolated from the neem tree, has attracted considerable interest due to its excellent performance in pest control. Studies have also reported pharmaceutical activities of dihydroniloticin, an intermediate in azadirachtin biosynthesis, but these pharmaceutical activities could not be validated due to the limited supply. In this study,
MeSH term(s)	Azadirachta/chemistry ; Azadirachta/genetics ; Melia azedarach ; Metabolic Engineering ; Molecular Docking Simulation ; Saccharomyces cerevisiae/genetics
Language	English
Publishing date	2022-03-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.1c07869
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Article: Metabolic Engineering of Saccharomyces cerevisiae for de Novo Dihydroniloticin Production Using Novel CYP450 from Neem (Azadirachta indica)

Hou, Kangxin / Yu, Wantong / Wang, Xiaojiao / Liu, Jiarou / Liu, Yan / Liu, Jia / Su, Xinyao / Zhang, Xiaoli / Xue, Qiang / Wang, Caixia

Journal of agricultural and food chemistry. 2022 Mar. 08, v. 70, no. 11

2022

Abstract	Azadirachtin, a limonoid isolated from the neem tree, has attracted considerable interest due to its excellent performance in pest control. Studies have also reported pharmaceutical activities of dihydroniloticin, an intermediate in azadirachtin biosynthesis, but these pharmaceutical activities could not be validated due to the limited supply. In this study, AiCYP71CD2 was first identified as involved in azadirachtin biosynthesis in neem by expressing it in Nicotiana benthamiana and yeast (Saccharomyces cerevisiae). Homology modeling and molecular docking analysis revealed that AiCYP71CD2 may exhibit a higher ability in catalyzing tirucalla-7,24-dien-3β-ol into dihydroniloticin compared with MaCYP71CD2 from Melia azedarach L. G310 was identified as the critical residue responsible for the higher catalytic ability of AiCYP71CD2. Condon-Optimized AiCYP71CD2 greatly improved the catalytic efficiency in yeast. De novo dihydroniloticin production using the novel AiCYP71CD2 was achieved by constructing the S. cerevisiae DI-3 strain, and the titer could reach up to 405 mg/L in a fermentor, which was an alternative source for dihydroniloticin.
Keywords	Azadirachta indica ; Melia azedarach ; Nicotiana benthamiana ; Saccharomyces cerevisiae ; azadirachtin ; biosynthesis ; catalytic activity ; fermenters ; food chemistry ; pest control ; trees ; yeasts
Language	English
Dates of publication	2022-0308
Size	p. 3467-3476.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.1c07869
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Corrigendum to "Co-production of polysaccharides, ginsenosides and succinic acid from panax ginseng residue: A typical industrial herbal waste" [Bioresource Technology 331 (2021) 125073].

Su, Xinyao / Xue, Qiang / Sun, Mengchu / Liu, Jiarou / Hung Wong, Ming / Wang, Caixia / Chen, Shilin

Bioresource technology

2021 Volume 341, Page(s) 125889

Language	English
Publishing date	2021-09-09
Publishing country	England
Document type	Published Erratum
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2021.125889
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Article ; Online: Co-production of polysaccharides, ginsenosides and succinic acid from Panax ginseng residue: A typical industrial herbal waste.

Su, Xinyao / Xue, Qiang / Sun, Mengchu / Liu, Jiarou / Wong, Ming Hung / Wang, Caixia / Chen, Shilin

Bioresource technology

2021 Volume 331, Page(s) 125073

Abstract: Co-production of polysaccharides, ginsenosides and succinic acid was achieved from Panax ginseng residue (PGR) in this study. Physico-chemical separation was first applied to recover the released polysaccharides and ginsenoside. Enzymatic hydrolysis was ... ...

Abstract	Co-production of polysaccharides, ginsenosides and succinic acid was achieved from Panax ginseng residue (PGR) in this study. Physico-chemical separation was first applied to recover the released polysaccharides and ginsenoside. Enzymatic hydrolysis was then conducted to covert the left PGR into mono-sugars which was following transformed into succinic acid by constructing a succinic acid-producing strain of Escherichia coli-ZW333. Results indicated that the yields of polysaccharides and ginsenosides increased according to the increase of deconstruction content of PGR. A total sugar yield reached 52 g/L at 10% PGR loading and increased to 94.33 g/L following fed-batch enzymatic hydrolysis. Finally, 56.28 g/L succinic acid was produced. In total, 18 g ginseng polysaccharides, 230 mg ginsenosides and 39 g succinic acid were produced from 100 g PGR. Accordingly, the total economic output could reach RMB 80,149 from 1 t PGR, illustrating the great value increasement of PGR by this industrially possible process.
MeSH term(s)	Ginsenosides ; Industrial Waste ; Panax ; Polysaccharides ; Succinic Acid
Chemical Substances	Ginsenosides ; Industrial Waste ; Polysaccharides ; Succinic Acid (AB6MNQ6J6L)
Language	English
Publishing date	2021-03-30
Publishing country	England
Document type	Journal Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2021.125073
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Article: [Recent advances of synthetic biology for production of functional ingredients in Chinese materia medica].

Su, Xin-Yao / Xue, Jian-Ping / Wang, Cai-Xia

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2016 Volume 41, Issue 22, Page(s) 4150–4157

Abstract: The functional ingredients in Chinese materia medica are the main active substance for traditional Chinese medicine and most of them are secondary metabolites derivatives. Until now,the main method to obtain those functional ingredients is through direct ...

Abstract	The functional ingredients in Chinese materia medica are the main active substance for traditional Chinese medicine and most of them are secondary metabolites derivatives. Until now,the main method to obtain those functional ingredients is through direct extraction from the Chinese materia medica. However, the income is very low because of the high extraction costs and the decreased medicinal plants. Synthetic biology technology, as a new and microbial approach, can be able to carry out large-scale production of functional ingredients and greatly ease the shortage of traditional Chinese medicine ingredients. This review mainly focused on the recent advances in synthetic biology for the functional ingredients production.
Language	Chinese
Publishing date	2016-11
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.4268/cjcmm20162211
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Article: [Regulation of β-mercuryl alcohol metabolic flow in Saccharomyces cerevisiae cells].

Chao, Er-Kun / Qian, Guang-Tao / Sun, Meng-Chu / Su, Xin-Yao / Zhu, Zhi-Hui / Sheng, Wei / Wang, Cai-Xia / Xue, Jian-Ping

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2020 Volume 45, Issue 16, Page(s) 3819–3825

Abstract: In this study, citrate synthase gene(CIT2), and malate synthase gene(MLS1) were successfully knocked out in β-amyrin-producing yeast cells by using CRISPR/CAS9. The promoter of phosphoglucose isomerase gene(PGI1) was replaced by that of cytochrome c ... ...

Abstract	In this study, citrate synthase gene(CIT2), and malate synthase gene(MLS1) were successfully knocked out in β-amyrin-producing yeast cells by using CRISPR/CAS9. The promoter of phosphoglucose isomerase gene(PGI1) was replaced by that of cytochrome c oxidase subunit Ⅶa(Cox9)to weaken its expression, aiming to channel more carbon flux into the NADPH-producing pathway. The fermentation results showed that CIT2 deletion had no effect on the β-amyrin production. Compared with the control strain, the production of β-amyrin was increased by 1.85 times after deleting MLS1, reaching into 3.3 mg·L~(-1). By replacing the promoter of PGI1, the β-amyrin yield was 3.75 times higher than that of the control strain, reaching up to 6.7 mg·L~(-1). This study successfully knocked out the CITT2 and MLS1 genes and weakened the PGI1 gene by using CRISPR/CAS9, which directly influenced the production of β-amyrin and provided some reference for the the metabolic engineering of triterpernoid producing strain.
MeSH term(s)	Ethanol ; Fermentation ; Metabolic Engineering ; Saccharomyces cerevisiae/genetics
Chemical Substances	Ethanol (3K9958V90M)
Language	Chinese
Publishing date	2020-09-07
Publishing country	China
Document type	Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.19540/j.cnki.cjcmm.20200506.112
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