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Article ; Online: 4D-printed needle panel meters coupled with enzymatic derivatization for reading urea and glucose concentrations in biological samples.

Wu, Chun-Yi / Su, Yi-Ting / Su, Cheng-Kuan

2023 Volume 237, Page(s) 115500

Abstract: On-site analytical techniques continue being developed with advances in modern technology. To demonstrate the applicability of four-dimensional printing (4DP) technologies in the direct fabrication of stimuli-responsive analytical devices for on-site ... ...

Abstract	On-site analytical techniques continue being developed with advances in modern technology. To demonstrate the applicability of four-dimensional printing (4DP) technologies in the direct fabrication of stimuli-responsive analytical devices for on-site determination of urea and glucose, we used digital light processing three-dimensional printing (3DP) and 2-carboxyethyl acrylate (CEA)-incorporated photocurable resins to fabricate all-in-one needle panel meters. When adding a sample having a value of pH above the pK
MeSH term(s)	Animals ; Humans ; Rats ; Biosensing Techniques/methods ; Glucose/analysis ; Printing, Three-Dimensional ; Reproducibility of Results ; Urea
Chemical Substances	Glucose (IY9XDZ35W2) ; Urea (8W8T17847W)
Language	English
Publishing date	2023-06-26
Publishing country	England
Document type	Journal Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2023.115500
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Article ; Online: 4D-printed needle panel meters coupled with enzymatic derivatization for reading urea and glucose concentrations in biological samples

Wu, Chun-Yi / Su, Yi-Ting / Su, Cheng-Kuan

Biosensors and Bioelectronics. 2023 Oct., v. 237 p.115500-

2023

Abstract	On-site analytical techniques continue being developed with advances in modern technology. To demonstrate the applicability of four-dimensional printing (4DP) technologies in the direct fabrication of stimuli-responsive analytical devices for on-site determination of urea and glucose, we used digital light processing three-dimensional printing (3DP) and 2-carboxyethyl acrylate (CEA)–incorporated photocurable resins to fabricate all-in-one needle panel meters. When adding a sample having a value of pH above the pKₐ of CEA (ca. 4.6–5.0) into the fabricated needle panel meter, the [H⁺]-responsive layer of the needle, printed using the CEA-incorporated photocurable resins, swelled as a result of electrostatic repulsion among the dissociated carboxyl groups of the copolymer, leading to [H⁺]-dependent bending of the needle. When coupled with a derivatization reaction (urease-mediated hydrolysis of urea to decrease [H⁺]; glucose oxidase-mediated oxidization of glucose to increase [H⁺]), the bending of the needle allowed reliable quantification of urea or glucose when referencing pre-calibrated concentration scales. After method optimization, the method's detection limits for urea and glucose were 4.9 and 7.0 μM, respectively, within a working concentration range from 0.1 to 10 mM. We verified the reliability of this analytical method by determining the concentrations of urea and glucose in samples of human urine, fetal bovine serum, and rat plasma with spike analyses and comparing the results with those obtained using commercial assay kits. Our results confirm that 4DP technologies can allow the direct fabrication of stimuli-responsive devices for quantitative chemical analysis, and that they can advance the development and applicability of 3DP-enabling analytical methods.
Keywords	biosensors ; chemical analysis ; composite polymers ; derivatization ; electrostatic interactions ; fetal bovine serum ; glucose ; humans ; hydrolysis ; pH ; rats ; urea ; urine ; Enzymatic derivatization ; Four-dimensional printing ; Stimuli-responsive materials ; Three-dimensional printing
Language	English
Dates of publication	2023-10
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2023.115500
Database	NAL-Catalogue (AGRICOLA)

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Article: Acute Appearance of a Neck Mass in an 11-Year-Old Girl.

Chiang, Ming-Hsiu / Su, Yi-Ting / Huang, Liang-Ti

Pediatric reports

2020 Volume 12, Issue 3, Page(s) 98–102

Abstract: Pustular abscess formation in the parotid gland is a rare complication following mumps virus infection. This is the second case report of pediatric parotid pustular abscess accompanied with mumps virus infection. Continuous antibiotics prescription ... ...

Abstract	Pustular abscess formation in the parotid gland is a rare complication following mumps virus infection. This is the second case report of pediatric parotid pustular abscess accompanied with mumps virus infection. Continuous antibiotics prescription without surgery is an eligible treatment for this patient.
Language	English
Publishing date	2020-10-27
Publishing country	Switzerland
Document type	Case Reports
ISSN	2036-749X
ISSN	2036-749X
DOI	10.3390/pediatric12030022
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Article ; Online: Comparison of add-on medications for persistent storage symptoms after α-blocker treatment in BPH patients - a network meta-analysis.

Su, Yi-Ting / Chen, Hsiao-Ling / Teoh, Jeremy Yuen-Chun / Chan, Vinson Wai-Shun / Wu, Wen-Jeng / Lee, Hsiang-Ying

BMC urology

2023 Volume 23, Issue 1, Page(s) 154

Abstract: Background: Patients with benign prostatic hyperplasia (BPH) receive α-blockers as first-line therapy to treat lower urinary tract symptoms; however, some individuals still experience residual storage symptoms. Antimuscarinics, β3-agonists, and ... ...

Abstract	Background: Patients with benign prostatic hyperplasia (BPH) receive α-blockers as first-line therapy to treat lower urinary tract symptoms; however, some individuals still experience residual storage symptoms. Antimuscarinics, β3-agonists, and desmopressin are effective add-on medications. Nevertheless, there is currently no evidence for the appropriate choice of the first add-on medication. This systematic review aimed to investigate the clinical benefits of antimuscarinics, β3-agonists, and desmopressin, in addition to α-blockers, for persistent storage symptoms in BPH patients. Methods: A comprehensive literature search of randomized controlled trials (RCTs) comparing the efficacy of different add-on medications in BPH patients with persistent storage symptoms despite α-blocker treatment was conducted. Clinical outcomes included the International Prostate Symptom Score (IPSS), IPSS storage subscore, nocturia, micturition, and urgency. A network meta-analysis was performed to estimate the effect size. Surface under cumulative ranking curves (SUCRAs) were used to rank the included treatments for each outcome. Results: A total of 15 RCTs were identified. Add-on imidafenacin and mirabegron resulted in significant improvement in all outcomes assessed. Other add-on medications such as desmopressin, tolterodine, solifenacin, fesoterodine, and propiverine showed positive benefits for most, but not all, outcomes. Based on the SUCRA rankings, add-on desmopressin was the best-ranked treatment for IPSS and nocturia, and add-on imidafenacin was the best for the IPSS storage subscore and micturition. Conclusions: BPH patients presenting with persistent storage symptoms despite α-blocker administration are recommended to include additional treatment. Desmopressin and imidafenacin may be considered high-priority add-on treatments because of their superior efficacy compared with other medications.
MeSH term(s)	Male ; Humans ; Muscarinic Antagonists/therapeutic use ; Prostatic Hyperplasia/complications ; Prostatic Hyperplasia/drug therapy ; Nocturia ; Network Meta-Analysis ; Deamino Arginine Vasopressin/therapeutic use ; Treatment Outcome ; Drug Therapy, Combination ; Lower Urinary Tract Symptoms/drug therapy ; Lower Urinary Tract Symptoms/etiology ; Adrenergic alpha-Antagonists/therapeutic use
Chemical Substances	Muscarinic Antagonists ; Deamino Arginine Vasopressin (ENR1LLB0FP) ; Adrenergic alpha-Antagonists
Language	English
Publishing date	2023-10-03
Publishing country	England
Document type	Systematic Review ; Meta-Analysis ; Journal Article
ZDB-ID	2059857-9
ISSN	1471-2490 ; 1471-2490
ISSN (online)	1471-2490
ISSN	1471-2490
DOI	10.1186/s12894-023-01327-1
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Article ; Online: A global database of bird nest traits.

Chia, Stephanie Yuan / Fang, Yi-Ting / Su, Yi-Ting / Tsai, Pei-Yu / Hsieh, Chia / Tsao, Shu-Han / Juang, Jia-Yang / Hung, Chih-Ming / Tuanmu, Mao-Ning

Scientific data

2023 Volume 10, Issue 1, Page(s) 923

Abstract: The reproductive success of birds is closely tied to the characteristics of their nests. It is crucial to understand the distribution of nest traits across phylogenetic and geographic dimensions to gain insight into bird evolution and adaptation. Despite ...

Abstract	The reproductive success of birds is closely tied to the characteristics of their nests. It is crucial to understand the distribution of nest traits across phylogenetic and geographic dimensions to gain insight into bird evolution and adaptation. Despite the extensive historical documentation on breeding behavior, a structured dataset describing bird nest characteristics has been lacking. To address this gap, we have compiled a comprehensive dataset that characterizes three ecologically and evolutionarily significant nest traits-site, structure, and attachment-for 9,248 bird species, representing all 36 orders and 241 out of the 244 families. By defining seven sites, seven structures, and four attachment types, we have systematically classified the nests of each species using information from text descriptions, photos, and videos sourced from online databases and literature. This nest traits dataset serves as a valuable addition to the existing body of morphological and ecological trait data for bird species, providing a useful resource for a wide range of avian macroecological and macroevolutionary research.
MeSH term(s)	Animals ; Birds ; Breeding ; Nesting Behavior ; Phylogeny ; Reproduction
Language	English
Publishing date	2023-12-21
Publishing country	England
Document type	Dataset
ZDB-ID	2775191-0
ISSN	2052-4463 ; 2052-4463
ISSN (online)	2052-4463
ISSN	2052-4463
DOI	10.1038/s41597-023-02837-1
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Article ; Online: Transcriptional regulation of the fidaxomicin gene cluster and cellular development in

Xie, Huang / Ruan, Jing-Yi / Bu, Qing-Ting / Li, Yue-Ping / Su, Yi-Ting / Zhao, Qing-Wei / Du, Yi-Ling / Li, Yong-Quan

Microbiology spectrum

2023 Volume 11, Issue 6, Page(s) e0270223

Abstract: Importance: Cascade regulation networks are almost present in various kinds of microorganisms, but locating and systematically elucidating specific pleiotropic regulators related to a certain gene cluster can be a tricky problem. Here, based on the ... ...

Abstract	Importance: Cascade regulation networks are almost present in various kinds of microorganisms, but locating and systematically elucidating specific pleiotropic regulators related to a certain gene cluster can be a tricky problem. Here, based on the promoter of the fidaxomicin pathway-specific regulator FadR1, we utilized a "DNA to Proteins" affinity purification method and captured a global regulator MtrA, which positively regulates fidaxomicin biosynthesis. In the
MeSH term(s)	Fidaxomicin ; ATP-Binding Cassette Transporters/genetics ; ATP-Binding Cassette Transporters/metabolism ; Gene Expression Regulation, Bacterial ; Multigene Family ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
Chemical Substances	Fidaxomicin (Z5N076G8YQ) ; ATP-Binding Cassette Transporters ; Bacterial Proteins
Language	English
Publishing date	2023-11-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.02702-23
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Article ; Online: Prediction of 5-Year Weight Loss and Weight Regain According to Early Weight Loss after Sleeve Gastrectomy.

Su, Yi-Ting / Su, Yen-Hao / Tam, Ka-Wai / Yen, Yu-Chun / Wang, Weu / Huang, Ming-Te / Wang, Shih-Yun / Pai, Fang-Yi / Kuo, Chih-Ying / Shen, Shih-Chiang

Obesity surgery

2023 Volume 33, Issue 5, Page(s) 1366–1372

Abstract: Background: Patients with morbid obesity exhibit sustained weight loss after sleeve gastrectomy (SG), but some individuals exhibit subsequent weight regain in the following years. Early weight loss was proven as a predictor of short- and mid-term weight ...

Abstract	Background: Patients with morbid obesity exhibit sustained weight loss after sleeve gastrectomy (SG), but some individuals exhibit subsequent weight regain in the following years. Early weight loss was proven as a predictor of short- and mid-term weight loss and regain. However, the long-term effects of early weight loss have yet to be fully investigated. This study investigated the predictive effects of early weight loss on long-term weight loss and regain after SG. Methods: Data of patients who underwent SG from November 2011 to July 2016 and followed through July 2021 were collected retrospectively. Weight regain was defined by weight increase more than 25% of their lost weight at the first postoperative year. Linear regression analysis and Cox proportional hazards analysis were performed to evaluate the correlations among early weight loss, weight loss, and weight regain. Results: Data of 408 patients were included. The percentages of total weight loss (%TWL) at postoperative months 1, 3, 12, and 60 were 10.6%, 18.1%, 29.3%, and 26.6%, respectively. The %TWL at months 1 and 3 were significantly correlated with %TWL after 5 years (P < .01). The weight regain rate was 29.8% at 5 years. The %TWL at months 1 and 3 significantly influenced weight regain (hazard ratio: 0.87 and 0.89, P = .017 and .008). Conclusion: Early weight loss may be used to predict weight loss and regain 5 years after SG. Patients with poor early weight loss are recommended to receive early interventions to achieve long-term weight loss and prevent weight regain.
MeSH term(s)	Humans ; Obesity, Morbid/surgery ; Retrospective Studies ; Gastrectomy ; Weight Loss ; Weight Gain ; Treatment Outcome ; Laparoscopy ; Gastric Bypass
Language	English
Publishing date	2023-03-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	1070827-3
ISSN	1708-0428 ; 0960-8923
ISSN (online)	1708-0428
ISSN	0960-8923
DOI	10.1007/s11695-023-06527-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Rational engineering strategies for achieving high-yield, high-quality and high-stability of natural product production in actinomycetes.

Bu, Qing-Ting / Li, Yue-Ping / Xie, Huang / Li, Ji-Feng / Lv, Zhong-Yuan / Su, Yi-Ting / Li, Yong-Quan

Metabolic engineering

2021 Volume 67, Page(s) 198–215

Abstract: Actinomycetes are recognized as excellent producers of microbial natural products, which have a wide range of applications, especially in medicine, agriculture and stockbreeding. The three main indexes of industrialization (titer, purity and stability) ... ...

Abstract	Actinomycetes are recognized as excellent producers of microbial natural products, which have a wide range of applications, especially in medicine, agriculture and stockbreeding. The three main indexes of industrialization (titer, purity and stability) must be taken into overall consideration in the manufacturing process of natural products. Over the past decades, synthetic biology techniques have expedited the development of industrially competitive strains with excellent performances. Here, we summarize various rational engineering strategies for upgrading the performance of industrial actinomycetes, which include enhancing the yield of natural products, eliminating the by-products and improving the genetic stability of engineered strains. Furthermore, the current challenges and future perspectives for optimizing the industrial strains more systematically through combinatorial engineering strategies are also discussed.
MeSH term(s)	Actinobacteria/genetics ; Actinomyces ; Biological Products ; Metabolic Engineering ; Synthetic Biology
Chemical Substances	Biological Products
Language	English
Publishing date	2021-06-22
Publishing country	Belgium
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1470383-x
ISSN	1096-7184 ; 1096-7176
ISSN (online)	1096-7184
ISSN	1096-7176
DOI	10.1016/j.ymben.2021.06.003
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Article ; Online: Genome-based rational engineering of Actinoplanes deccanensis for improving fidaxomicin production and genetic stability.

Li, Yue-Ping / Bu, Qing-Ting / Li, Ji-Feng / Xie, Huang / Su, Yi-Ting / Du, Yi-Ling / Li, Yong-Quan

Bioresource technology

2021 Volume 330, Page(s) 124982

Abstract: Microbial fermentation is currently still the major way to produce structural complicated clinical drugs. Yet, the low productivity and genetic instability of producing strains remain the bottlenecks in microbial pharmaceutical industry. Fidaxomicin is a ...

Abstract	Microbial fermentation is currently still the major way to produce structural complicated clinical drugs. Yet, the low productivity and genetic instability of producing strains remain the bottlenecks in microbial pharmaceutical industry. Fidaxomicin is a microbial drug against the Clostridium difficile infection. Here, a genome-based combinatorial engineering strategy was established to improve both fidaxomicin production and the genetic stability of Actinoplanes deccanensis YP-1. Guided by genomic analysis, several genetic instability-associated elements were cumulatively deleted, generating a more genetically stable mutant. Further rational engineering approaches including elimination of a pigment pathway, duplication of the fidaxomicin gene cluster, overexpression of a positive regulator and optimization of the fermentation medium, led to an overall 27-folds improvement in fidaxomicin production. Taken together, the genome-based rational combinatorial engineering strategy was efficient to enhance the fidaxomicin production and ameliorate the genetic stability of YP-1, it can also be widely used in other industrial actinomycetes for strain improvement.
MeSH term(s)	Actinoplanes ; Aminoglycosides ; Anti-Bacterial Agents ; Clostridioides difficile ; Fidaxomicin
Chemical Substances	Aminoglycosides ; Anti-Bacterial Agents ; Fidaxomicin (Z5N076G8YQ)
Language	English
Publishing date	2021-03-13
Publishing country	England
Document type	Journal Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2021.124982
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Article: Rational engineering strategies for achieving high-yield, high-quality and high-stability of natural product production in actinomycetes

Bu, Qing-Ting / Li, Yue-Ping / Xie, Huang / Li, Ji-Feng / Lv, Zhong-Yuan / Su, Yi-Ting / Li, Yong-Quan

International Metabolic Engineering Society Metabolic engineering. 2021 Sept., v. 67

2021

Abstract	Actinomycetes are recognized as excellent producers of microbial natural products, which have a wide range of applications, especially in medicine, agriculture and stockbreeding. The three main indexes of industrialization (titer, purity and stability) must be taken into overall consideration in the manufacturing process of natural products. Over the past decades, synthetic biology techniques have expedited the development of industrially competitive strains with excellent performances. Here, we summarize various rational engineering strategies for upgrading the performance of industrial actinomycetes, which include enhancing the yield of natural products, eliminating the by-products and improving the genetic stability of engineered strains. Furthermore, the current challenges and future perspectives for optimizing the industrial strains more systematically through combinatorial engineering strategies are also discussed.
Keywords	Actinobacteria ; genetic stability ; industrialization ; medicine ; synthetic biology
Language	English
Dates of publication	2021-09
Size	p. 198-215.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	1470383-x
ISSN	1096-7184 ; 1096-7176
ISSN (online)	1096-7184
ISSN	1096-7176
DOI	10.1016/j.ymben.2021.06.003
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