LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 18

Search options

  1. Article ; Online: Long stem revision versus short stem revision with plate osteosynthesis for Vancouver type B2 periprosthetic femoral fracture: a comparative study of eighty five cases.

    Chen, Jian-Jiun / Hung, Shih-Hsin / Liou, Jia-You / Chang, Wen-Chieh / Hsu, Kuei-Hsiang / Su, Yu-Pin / Chiu, Fang-Yao / Cheng, Ming-Fai

    International orthopaedics

    2024  

    Abstract: Purpose: Periprosthetic femoral fractures (PPFs) around the hip are challenging complications in orthopaedic surgery, particularly Vancouver type B2 (VTB2) fractures. The surgical management of these fractures is crucial and depends on various factors. ... ...

    Abstract Purpose: Periprosthetic femoral fractures (PPFs) around the hip are challenging complications in orthopaedic surgery, particularly Vancouver type B2 (VTB2) fractures. The surgical management of these fractures is crucial and depends on various factors. Cementless short taper stem with plate osteosynthesis is an alternative surgical technique. This study aims to compare the outcomes of this surgical technique with revision arthroplasty (RA) with long stem in the treatment of VTB2 PPFs.
    Methods: This retrospective study was conducted in a single medical institute from February 2010 to May 2019. Patients who had received either total hip arthroplasty or bipolar hemiarthroplasty and subsequently developed a VTB2 PPF were included; patients who sustained intra-operative fractures or received a cemented stem previously were excluded from the analysis. The patients were divided into two groups: group I received RA with cementless long stem, while group II underwent RA with cementless short taper stem with plate osteosynthesis. Demographic data, radiographic and functional outcomes, and complications were analyzed between the two groups.
    Results: A total of 85 patients diagnosed with VTB2 PPFs were included in the study. There were no significant differences between the two groups in terms of demographic data, including age, gender, mean follow-up times, estimated blood loss, and operative times. The radiographic results showed that there was no significant difference in the incidence of subsidence and implant stability between the two groups. However, group II tended to have less subsidence and periprosthetic osteolysis. Patients in group II had significantly better functional scores (mean Harris hip score: post-operative: 60.2 in group I and 66.7 in group ii; last follow-up: 77.4 in group 1 and 83.2 in group II (both p < 0.05)). There were no significant differences in the overall complication rate, including infection, dislocation, re-fracture, and revision surgery, between the two groups.
    Conclusions: Both surgical techniques, cementless long stem and cementless short taper stem with plate osteosynthesis, are effective in the treatment of Vancouver B2 PPFs, with no significant differences in outcomes or complications. However, patients in cementless short taper stem with plate osteosynthesis had better functional scores at both post-operative and the last follow-up.
    Language English
    Publishing date 2024-04-23
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 80384-4
    ISSN 1432-5195 ; 0341-2695
    ISSN (online) 1432-5195
    ISSN 0341-2695
    DOI 10.1007/s00264-024-06181-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  2. Article ; Online: Harnessing the Noncanonical Keap1-Nrf2 Pathway for Human Cytomegalovirus Control.

    Ghosh, Ayan K / Su, Yu-Pin / Forman, Michael / Keyes, Robert F / Smith, Brian C / Hu, Xin / Ferrer, Marc / Arav-Boger, Ravit

    Journal of virology

    2023  Volume 97, Issue 4, Page(s) e0016023

    Abstract: Host-derived cellular pathways can provide an unfavorable environment for virus replication. These pathways have been a subject of interest for herpesviruses, including the betaherpesvirus human cytomegalovirus (HCMV). Here, we demonstrate that a ... ...

    Abstract Host-derived cellular pathways can provide an unfavorable environment for virus replication. These pathways have been a subject of interest for herpesviruses, including the betaherpesvirus human cytomegalovirus (HCMV). Here, we demonstrate that a compound, ARP101, induces the noncanonical sequestosome 1 (SQSTM1)/p62-Keap1-Nrf2 pathway for HCMV suppression. ARP101 increased the levels of both LC3 II and SQSTM1/p62 and induced phosphorylation of p62 at the C-terminal domain, resulting in its increased affinity for Keap1. ARP101 treatment resulted in Nrf2 stabilization and translocation into the nucleus, binding to specific promoter sites and transcription of antioxidant enzymes under the antioxidant response element (ARE), and HCMV suppression. Knockdown of Nrf2 recovered HCMV replication following ARP101 treatment, indicating the role of the Keap1-Nrf2 axis in HCMV inhibition by ARP101. SQSTM1/p62 phosphorylation was not modulated by the mTOR kinase or casein kinase 1 or 2, indicating ARP101 engages other kinases. Together, the data uncover a novel antiviral strategy for SQSTM1/p62 through the noncanonical Keap1-Nrf2 axis. This pathway could be further exploited, including the identification of the responsible kinases, to define the biological events during HCMV replication.
    MeSH term(s) Cytomegalovirus/drug effects ; Cytomegalovirus/physiology ; Cytomegalovirus Infections/prevention & control ; Cytomegalovirus Infections/virology ; Antiviral Agents/pharmacology ; Virus Replication ; Transcription, Genetic/drug effects ; Phosphorylation/drug effects ; Antioxidant Response Elements/drug effects ; Cell Line ; Humans
    Chemical Substances ARP101 compound ; Antiviral Agents ; KEAP1 protein, human ; NFE2L2 protein, human ; SQSTM1 protein, human ; P62 protein, human
    Language English
    Publishing date 2023-03-20
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/jvi.00160-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  3. Article ; Online: Evaluating a Cobalt-Tetraphenylporphyrin Complex, Functionalized with a Reduced Graphene Oxide Nanocomposite, for Improved Tooth Whitening.

    Su, I-Hsuan / Lee, Chen-Fu / Su, Yu-Pin / Wang, Lai-Hao

    Journal of esthetic and restorative dentistry : official publication of the American Academy of Esthetic Dentistry ... [et al.

    2016  Volume 28, Issue 5, Page(s) 321–329

    Abstract: Objectives: To study using cobalt-tetraphenylporphyrin/reduced graphene oxide (CoTPP/RGO), a nanocomposite highly catalytic both for oxidizing and for reducing hydrogen peroxide (H: Methods: Dyes (D&C Red 34, Orange No. 4), tea, and betel nuts (Areca ...

    Abstract Objectives: To study using cobalt-tetraphenylporphyrin/reduced graphene oxide (CoTPP/RGO), a nanocomposite highly catalytic both for oxidizing and for reducing hydrogen peroxide (H
    Methods: Dyes (D&C Red 34, Orange No. 4), tea, and betel nuts (Areca catechu) were used to stain the tooth specimens for three days and subsequently bleached using H
    Results: CoTPP/RGO increased the tooth-whitening efficacy of H
    Clinical significance: First, the Co/TPP/RGO complex allows improved tooth bleaching and shorter treatment times. Second, because the Co/TPP/RGO complex bleached D&C Red 34 at a similar rate and to a similar degree as tea and other natural agents stain teeth, it is a convenient staining agent for studying many vital aspects of tooth bleaching. (J Esthet Restor Dent 28:321-329, 2016).
    MeSH term(s) Cobalt ; Color ; Graphite ; Hydrogen Peroxide ; Nanocomposites ; Porphyrins ; Tea ; Tooth ; Tooth Bleaching ; Tooth Bleaching Agents ; Tooth Discoloration
    Chemical Substances Porphyrins ; Tea ; Tooth Bleaching Agents ; tetraphenylporphyrin ; Cobalt (3G0H8C9362) ; Graphite (7782-42-5) ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2016
    Publishing country England
    Document type Journal Article
    ZDB-ID 2043146-6
    ISSN 1708-8240 ; 1496-4155
    ISSN (online) 1708-8240
    ISSN 1496-4155
    DOI 10.1111/jerd.12240
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  4. Article ; Online: Dependence of coronavirus RNA replication on an NH2-terminal partial nonstructural protein 1 in cis.

    Su, Yu-Pin / Fan, Yi-Hsin / Brian, David A

    Journal of virology

    2014  Volume 88, Issue 16, Page(s) 8868–8882

    Abstract: Unlabelled: Genomes of positive (+)-strand RNA viruses use cis-acting signals to direct both translation and replication. Here we examine two 5'-proximal cis-replication signals of different character in a defective interfering (DI) RNA of the bovine ... ...

    Abstract Unlabelled: Genomes of positive (+)-strand RNA viruses use cis-acting signals to direct both translation and replication. Here we examine two 5'-proximal cis-replication signals of different character in a defective interfering (DI) RNA of the bovine coronavirus (BCoV) that map within a 322-nucleotide (nt) sequence (136 nt from the genomic 5' untranslated region and 186 nt from the nonstructural protein 1 [nsp1]-coding region) not found in the otherwise-identical nonreplicating subgenomic mRNA7 (sgmRNA7). The natural DI RNA is structurally a fusion of the two ends of the BCoV genome that results in a single open reading frame between a partial nsp1-coding region and the entire N gene. (i) In the first examination, mutation analyses of a recently discovered long-range RNA-RNA base-paired structure between the 5' untranslated region and the partial nsp1-coding region showed that it, possibly in concert with adjacent stem-loops, is a cis-acting replication signal in the (+) strand. We postulate that the higher-order structure promotes (+)-strand synthesis. (ii) In the second examination, analyses of multiple frame shifts, truncations, and point mutations within the partial nsp1-coding region showed that synthesis of a PEFP core amino acid sequence within a group A lineage betacoronavirus-conserved NH2-proximal WAPEFPWM domain is required in cis for DI RNA replication. We postulate that the nascent protein, as part of an RNA-associated translating complex, acts to direct the DI RNA to a critical site, enabling RNA replication. We suggest that these results have implications for viral genome replication and explain, in part, why coronavirus sgmRNAs fail to replicate.
    Importance: cis-Acting RNA and protein structures that regulate (+)-strand RNA virus genome synthesis are potential sites for blocking virus replication. Here we describe two: a previously suspected 5'-proximal long-range higher-order RNA structure and a novel nascent NH2-terminal protein component of nsp1 that are common among betacoronaviruses of group A lineage.
    MeSH term(s) 5' Untranslated Regions/genetics ; Amino Acid Sequence/genetics ; Base Pairing/genetics ; Base Sequence/genetics ; Cell Line, Tumor ; Coronavirus Infections/genetics ; Coronavirus Infections/virology ; Coronavirus, Bovine/genetics ; Genome, Viral/genetics ; Humans ; Molecular Sequence Data ; Open Reading Frames/genetics ; RNA, Viral/genetics ; Viral Nonstructural Proteins/genetics ; Virus Replication/genetics
    Chemical Substances 5' Untranslated Regions ; RNA, Viral ; Viral Nonstructural Proteins
    Keywords covid19
    Language English
    Publishing date 2014-05-28
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.00738-14
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  5. Article ; Online: Protein detection in blood with single-molecule imaging.

    Mao, Chih-Ping / Wang, Shih-Chin / Su, Yu-Pin / Tseng, Ssu-Hsueh / He, Liangmei / Wu, Annie A / Roden, Richard B S / Xiao, Jie / Hung, Chien-Fu

    Science advances

    2021  Volume 7, Issue 33

    Abstract: The ability to characterize individual biomarker protein molecules in patient blood samples could enable diagnosis of diseases at an earlier stage, when treatment is typically more effective. Single-molecule imaging offers a promising approach to ... ...

    Abstract The ability to characterize individual biomarker protein molecules in patient blood samples could enable diagnosis of diseases at an earlier stage, when treatment is typically more effective. Single-molecule imaging offers a promising approach to accomplish this goal. However, thus far, single-molecule imaging methods have not been translated into the clinical setting. The detection limit of these methods has been confined to the picomolar (10
    MeSH term(s) Biomarkers/analysis ; Humans ; Nanotechnology ; Proteins/analysis ; Single Molecule Imaging/methods
    Chemical Substances Biomarkers ; Proteins
    Language English
    Publishing date 2021-08-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.abg6522
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  6. Article ; Online: Displaced isolated greater tuberosity fractures of elder adults treated with plate osteosynthesis.

    Cheng, Ming Fai / Hung, Shih-Hsin / Su, Yu-Pin / Chiang, Chao-Ching / Chang, Ming-Chau / Chiu, Fang-Yao

    Journal of the Chinese Medical Association : JCMA

    2019  Volume 82, Issue 4, Page(s) 318–321

    Abstract: Background: We elucidated the effect of open reduction and internal fixation with locking plate for acute isolated displaced greater tuberosity fractures of humerus in elder adults (aged >60 years).: Methods: From 2009 to 2015, data from 32 patients, ...

    Abstract Background: We elucidated the effect of open reduction and internal fixation with locking plate for acute isolated displaced greater tuberosity fractures of humerus in elder adults (aged >60 years).
    Methods: From 2009 to 2015, data from 32 patients, aged between 60 and 88 years, who had acute unilaterally displaced greater tuberosity fractures of humerus were collected and evaluated retrospectively. All the fractures were managed with open reduction and internal fixation with locking plate. The follow-up period was 50.8 months on an average (range 22-80 months). Finally, 25 patients were available for final evaluation of radiographic and functional results.
    Results: All the 32 fractures had union with the average union time of 14 weeks (range 10-18 weeks) and with no complications. The average of preoperative Visual Analogue Scale (VAS) was 6.2 (range 4-8), ASES was 30.4 (range 13-45), and Constant score was 30.4 (range 20-45). At the last follow-up, the mean VAS was 1.3 (range 0-2.5), the mean ASES score was 90.1 (range 72-100), and the mean Constant score was 90.3 (range 80-100). There were statistically significant differences between preoperative and final follow-up in VAS, ASES, and Constant score. Hundred percent of patients had good or excellent results by Constant score, with excellent results (86-100) in 17 (68%) patients and good result (71-85) in 8 (32%).
    Conclusion: In conclusion, open reduction and internal fixation with locking plate is an effective treatment for acute displaced greater tuberosity fractures of humerus in elder adults.
    MeSH term(s) Aged ; Aged, 80 and over ; Bone Plates ; Female ; Fracture Fixation, Internal/methods ; Humans ; Humeral Head ; Male ; Middle Aged ; Retrospective Studies ; Shoulder Fractures/surgery ; Visual Analog Scale
    Language English
    Publishing date 2019-04-04
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2107283-8
    ISSN 1728-7731 ; 1726-4901
    ISSN (online) 1728-7731
    ISSN 1726-4901
    DOI 10.1097/JCMA.0000000000000049
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  7. Article ; Online: Staged protocol for the treatment of chronic femoral shaft osteomyelitis with Ilizarov's technique followed by the use of intramedullary locked nail.

    Chou, Po-Hsin / Lin, Hsi-Hsien / Su, Yu-Pin / Chiang, Chao-Ching / Chang, Ming-Chau / Chen, Chuan-Mu

    Journal of the Chinese Medical Association : JCMA

    2017  Volume 80, Issue 6, Page(s) 376–382

    Abstract: Background: Infected nonunion of the femoral shaft is uncommon, and usually presents with challenging therapeutic and reconstructive problems. There are still controversies over treating infected nonunion of the femoral shaft. The purposes of this ... ...

    Abstract Background: Infected nonunion of the femoral shaft is uncommon, and usually presents with challenging therapeutic and reconstructive problems. There are still controversies over treating infected nonunion of the femoral shaft. The purposes of this retrospective study were to review the treatment outcomes and describe a staged protocol for spontaneous wound healing.
    Methods: Six patients with chronic femoral shaft infected-nonunion from October 2002 to September 2010 were included in this retrospective study. Serial plain films and triple films of lower legs were performed to evaluate the alignment of the treated femoral shaft and bony union following our staged protocol of Ilizarov distraction osteogenesis and intramedullary nailing.
    Results: An average bone defect of 7 cm was noted after staged osteotomy. Mean follow-up was 87.5 (range, 38-133) months. Union was achieved in all six patients, with an average external fixation time of 6.8 (range, 5-11) months. There was no reinfection. One complication of a 4-cm leg discrepancy was noted, with an initial shortening of 15 cm. The mean knee ranges of motion (ROM) before staged protocols and at final follow-up were 64.2±8.6 (range, 60-75)° and 53.3±9.3 (range, 40-65)°, respectively. The ROM at the knee joint statistically decreased following staged protocols.
    Conclusion: In the treatment of chronic femur osteomyelitis, the staged protocol of Ilizarov distraction osteogenesis followed by intramedullary nailing was safe and successful, and allowed for union, realignment, reorientation, and leg-length restoration. With regard to the soft tissue, this technique provides a unique type of reconstructive closure for infected wounds. It is suggested that the staged protocol is reliable in providing successful simultaneous reconstruction for bone and soft tissue defects without flap coverage.
    MeSH term(s) Adult ; Chronic Disease ; Clinical Protocols ; Female ; Femoral Fractures/complications ; Femoral Fractures/surgery ; Fracture Fixation, Intramedullary/methods ; Fractures, Ununited/complications ; Fractures, Ununited/surgery ; Humans ; Ilizarov Technique ; Male ; Middle Aged ; Osteomyelitis/surgery ; Retrospective Studies
    Language English
    Publishing date 2017-02-24
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2107283-8
    ISSN 1728-7731 ; 1726-4901
    ISSN (online) 1728-7731
    ISSN 1726-4901
    DOI 10.1016/j.jcma.2017.01.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  8. Article ; Online: Genetic evidence of a long-range RNA-RNA interaction between the genomic 5' untranslated region and the nonstructural protein 1 coding region in murine and bovine coronaviruses.

    Guan, Bo-Jhih / Su, Yu-Pin / Wu, Hung-Yi / Brian, David A

    Journal of virology

    2012  Volume 86, Issue 8, Page(s) 4631–4643

    Abstract: Higher-order RNA structures in the 5' untranslated regions (UTRs) of the mouse hepatitis coronavirus (MHV) and bovine coronavirus (BCoV), separate species in the betacoronavirus genus, appear to be largely conserved despite an ∼36% nucleotide sequence ... ...

    Abstract Higher-order RNA structures in the 5' untranslated regions (UTRs) of the mouse hepatitis coronavirus (MHV) and bovine coronavirus (BCoV), separate species in the betacoronavirus genus, appear to be largely conserved despite an ∼36% nucleotide sequence divergence. In a previous study, each of three 5'-end-proximal cis-acting stem-loop domains in the BCoV genome, I/II, III, and IV, yielded near-wild-type (wt) MHV phenotypes when used by reverse genetics to replace its counterpart in the MHV genome. Replacement with the BCoV 32-nucleotide (nt) inter-stem-loop fourth domain between stem-loops III and IV, however, required blind cell passaging for virus recovery. Here, we describe suppressor mutations within the transplanted BCoV 32-nt domain that along with appearance of potential base pairings identify an RNA-RNA interaction between this domain and a 32-nt region ∼200 nt downstream within the nonstructural protein 1 (Nsp1)-coding region. Mfold and phylogenetic covariation patterns among similarly grouped betacoronaviruses support this interaction, as does cotransplantation of the BCoV 5' UTR and its downstream base-pairing domain. Interestingly, cotransplantation of the BCoV 5' UTR and BCoV Nsp1 coding region directly yielded an MHV wt-like phenotype, which demonstrates a cognate interaction between these two BCoV regions, which in the MHV genome act in a fully interspecies-compliant manner. Surprisingly, the 30-nt inter-stem-loop domain in the MHV genome can be deleted and viral progeny, although debilitated, are still produced. These results together identify a previously undescribed long-range RNA-RNA interaction between the 5' UTR and Nsp1 coding region in MHV-like and BCoV-like betacoronaviruses that is cis acting for viral fitness but is not absolutely required for viral replication in cell culture.
    MeSH term(s) 5' Untranslated Regions ; Animals ; Base Pairing ; Base Sequence ; Cattle ; Cell Line ; Coronavirus/genetics ; Coronavirus, Bovine/genetics ; Cricetinae ; Genome, Viral ; Genotype ; Humans ; Inverted Repeat Sequences ; Mice ; Molecular Sequence Data ; Murine hepatitis virus/genetics ; Mutagenesis, Site-Directed ; Mutation ; Nucleic Acid Conformation ; Nucleotide Motifs ; Open Reading Frames ; Phenotype ; RNA, Viral/chemistry ; RNA, Viral/metabolism ; Sequence Alignment ; Viral Nonstructural Proteins/genetics ; Virus Replication
    Chemical Substances 5' Untranslated Regions ; RNA, Viral ; Viral Nonstructural Proteins
    Keywords covid19
    Language English
    Publishing date 2012-02-15
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.06265-11
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  9. Article ; Online: Reselection of a genomic upstream open reading frame in mouse hepatitis coronavirus 5'-untranslated-region mutants.

    Wu, Hung-Yi / Guan, Bo-Jhih / Su, Yu-Pin / Fan, Yi-Hsin / Brian, David A

    Journal of virology

    2013  Volume 88, Issue 2, Page(s) 846–858

    Abstract: An AUG-initiated upstream open reading frame (uORF) encoding a potential polypeptide of 3 to 13 amino acids (aa) is found within the 5' untranslated region (UTR) of >75% of coronavirus genomes based on 38 reference strains. Potential CUG-initiated uORFs ... ...

    Abstract An AUG-initiated upstream open reading frame (uORF) encoding a potential polypeptide of 3 to 13 amino acids (aa) is found within the 5' untranslated region (UTR) of >75% of coronavirus genomes based on 38 reference strains. Potential CUG-initiated uORFs are also found in many strains. The AUG-initiated uORF is presumably translated following genomic 5'-end cap-dependent ribosomal scanning, but its function is unknown. Here, in a reverse-genetics study with mouse hepatitis coronavirus, the following were observed. (i) When the uORF AUG-initiating codon was replaced with a UAG stop codon along with a U112A mutation to maintain a uORF-harboring stem-loop 4 structure, an unimpaired virus with wild-type (WT) growth kinetics was recovered. However, reversion was found at all mutated sites within five virus passages. (ii) When the uORF was fused with genomic (main) ORF1 by converting three in-frame stop codons to nonstop codons, a uORF-ORF1 fusion protein was made, and virus replicated at WT levels. However, a frameshifting G insertion at virus passage 7 established a slightly 5'-extended original uORF. (iii) When uAUG-eliminating deletions of 20, 30, or 51 nucleotides (nt) were made within stem-loop 4, viable but debilitated virus was recovered. However, a C80U mutation in the first mutant and an A77G mutation in the second appeared by passage 10, which generated alternate uORFs that correlated with restored WT growth kinetics. In vitro, the uORF-disrupting nondeletion mutants showed enhanced translation of the downstream ORF1 compared with the WT. These results together suggest that the uORF represses ORF1 translation yet plays a beneficial but nonessential role in coronavirus replication in cell culture.
    MeSH term(s) 5' Untranslated Regions ; Animals ; Cell Line ; Codon, Initiator ; Coronavirus/genetics ; Coronavirus/metabolism ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Genome, Viral ; Mice ; Mutation ; Open Reading Frames ; Point Mutation ; Protein Biosynthesis ; Rodent Diseases/virology
    Chemical Substances 5' Untranslated Regions ; Codon, Initiator
    Keywords covid19
    Language English
    Publishing date 2013-10-30
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.02831-13
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  10. Article ; Online: Inhibition of Cytomegalovirus Replication with Extended-Half-Life Synthetic Ozonides.

    Wang, Yiping / Mukhopadhyay, Rupkatha / Roy, Sujayita / Kapoor, Arun / Su, Yu-Pin / Charman, Susan A / Chen, Gong / Wu, Jianbo / Wang, Xiaofang / Vennerstrom, Jonathan L / Arav-Boger, Ravit

    Antimicrobial agents and chemotherapy

    2018  Volume 63, Issue 1

    Abstract: Artesunate (AS), a semisynthetic artemisinin approved for malaria therapy, inhibits human cytomegalovirus (HCMV) ... ...

    Abstract Artesunate (AS), a semisynthetic artemisinin approved for malaria therapy, inhibits human cytomegalovirus (HCMV) replication
    MeSH term(s) Animals ; Antiviral Agents/blood ; Antiviral Agents/chemistry ; Antiviral Agents/pharmacokinetics ; Antiviral Agents/pharmacology ; CDC2 Protein Kinase/genetics ; CDC2 Protein Kinase/metabolism ; Cell Line ; Cytomegalovirus/drug effects ; Cytomegalovirus/genetics ; Cytomegalovirus/metabolism ; Cytomegalovirus Infections/drug therapy ; Cytomegalovirus Infections/virology ; Drug Administration Schedule ; Drug Evaluation, Preclinical ; Drug Resistance, Viral/drug effects ; Drug Resistance, Viral/genetics ; E2F1 Transcription Factor/genetics ; E2F1 Transcription Factor/metabolism ; Female ; Fibroblasts/drug effects ; Fibroblasts/pathology ; Fibroblasts/virology ; Ganciclovir/pharmacology ; Gene Expression Regulation ; Heterocyclic Compounds, 1-Ring/blood ; Heterocyclic Compounds, 1-Ring/chemistry ; Heterocyclic Compounds, 1-Ring/pharmacokinetics ; Heterocyclic Compounds, 1-Ring/pharmacology ; Host-Pathogen Interactions/drug effects ; Humans ; Isoenzymes/genetics ; Isoenzymes/metabolism ; Male ; Mice ; Mice, Inbred BALB C ; Microbial Sensitivity Tests ; Retinoblastoma Protein/genetics ; Retinoblastoma Protein/metabolism ; Signal Transduction ; Spiro Compounds/blood ; Spiro Compounds/chemistry ; Spiro Compounds/pharmacokinetics ; Spiro Compounds/pharmacology ; Virus Replication/drug effects
    Chemical Substances Antiviral Agents ; E2F1 Transcription Factor ; E2F1 protein, human ; Heterocyclic Compounds, 1-Ring ; Isoenzymes ; OZ418 compound ; Retinoblastoma Protein ; Spiro Compounds ; CDC2 Protein Kinase (EC 2.7.11.22) ; CDK1 protein, human (EC 2.7.11.22) ; Ganciclovir (P9G3CKZ4P5)
    Language English
    Publishing date 2018-12-21
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 217602-6
    ISSN 1098-6596 ; 0066-4804
    ISSN (online) 1098-6596
    ISSN 0066-4804
    DOI 10.1128/AAC.01735-18
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

To top