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  1. Article ; Online: Quarter-Century PET/Computed Tomography Transformation of Oncology: Hepatobiliary and Pancreatic Cancer.

    Kandathil, Asha / Subramaniam, Rathan

    PET clinics

    2024  Volume 19, Issue 2, Page(s) 163–175

    Abstract: 18F] Fluorodeoxyglucose ( ...

    Abstract [18F] Fluorodeoxyglucose (
    MeSH term(s) Male ; Humans ; Positron Emission Tomography Computed Tomography ; Fluorodeoxyglucose F18 ; Gallium Radioisotopes ; Tomography, X-Ray Computed ; Pancreatic Neoplasms/diagnostic imaging
    Chemical Substances Fluorodeoxyglucose F18 (0Z5B2CJX4D) ; Gallium Radioisotopes
    Language English
    Publishing date 2024-01-11
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2764575-7
    ISSN 1879-9809 ; 1556-8598
    ISSN (online) 1879-9809
    ISSN 1556-8598
    DOI 10.1016/j.cpet.2023.12.003
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  2. Article ; Online: Allele mining, amplicon sequencing and computational prediction of Solanum melongena L. FT/TFL1 gene homologs uncovers putative variants associated to seed dormancy and germination.

    Subramaniam, Ranjita / Kumar, Vijay Subbiah

    PloS one

    2023  Volume 18, Issue 5, Page(s) e0285119

    Abstract: The FT/TFL1 gene homolog family plays a crucial role in the regulation of floral induction, seed dormancy and germination in angiosperms. Despite its importance, the FT/TFL1 gene homologs in eggplant (Solanum melongena L.) have not been characterized to ... ...

    Abstract The FT/TFL1 gene homolog family plays a crucial role in the regulation of floral induction, seed dormancy and germination in angiosperms. Despite its importance, the FT/TFL1 gene homologs in eggplant (Solanum melongena L.) have not been characterized to date. In this study, we performed a genome-wide identification of FT/TFL1 genes in eggplant using in silico genome mining. The presence of these genes was validated in four economically important eggplant cultivars (Surya, EP-47 Annamalai, Pant Samrat and Arka Nidhi) through Pacbio RSII amplicon sequencing. Our results revealed the presence of 12 FT/TFL1 gene homologs in eggplant, with evidence of diversification among FT-like genes suggesting their possible adaptations towards various environmental stimuli. The amplicon sequencing also revealed the presence of two alleles for certain genes (SmCEN-1, SmCEN-2, SmMFT-1 and SmMFT-2) of which SmMFT-2 was associated with seed dormancy and germination. This association was further supported by the observation that seed dormancy is rarely reported in domesticated eggplant cultivars, but is commonly observed in wild species. A survey of the genetic regions in domesticated cultivars and a related wild species, S. incanum, showed that the alternative allele of S. incanum was present in some members of the Pant Samrat cultivar, but was absent in most other cultivars. This difference could contribute to the differences in seed traits between wild and domesticated eggplants.
    MeSH term(s) Solanum melongena/genetics ; Alleles ; Plant Dormancy/genetics ; Seeds/genetics ; Phenotype ; Gene Expression Regulation, Plant
    Language English
    Publishing date 2023-05-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0285119
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  3. Article ; Online: A Bioinformatic Guide to Identify Protein Effectors from Phytopathogens.

    Blackman, Christopher / Subramaniam, Rajagopal

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2659, Page(s) 95–101

    Abstract: Phytopathogenic fungi are a diverse and widespread group that has a significant detrimental impact on crops with an estimated annual average loss of 15% worldwide. Understanding the interaction between host plants and pathogenic fungi is critical to ... ...

    Abstract Phytopathogenic fungi are a diverse and widespread group that has a significant detrimental impact on crops with an estimated annual average loss of 15% worldwide. Understanding the interaction between host plants and pathogenic fungi is critical to delineate underlying mechanisms of plant defense to mitigate agricultural losses. Fungal pathogens utilize suites of secreted molecules, called effectors, to modulate plant metabolism and immune response to overcome host defenses and promote colonization. Effectors come in many flavors including proteinaceous products, small RNAs, and metabolites such as mycotoxins. This review will focus on methods for identifying protein effectors from fungi. Excellent reviews have been published to identify secondary metabolites and small RNAs from fungi and therefore will not be part of this review.
    MeSH term(s) Plant Diseases/microbiology ; Fungi/chemistry ; Fungi/classification ; Fungi/metabolism ; Secretome ; Computational Biology/methods ; Fungal Proteins/analysis ; Machine Learning ; Host Microbial Interactions
    Chemical Substances Fungal Proteins
    Language English
    Publishing date 2023-05-30
    Publishing country United States
    Document type Review ; Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3159-1_8
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  4. Article: Robotic Approach to Creation of Continent Catheterisable Channels-Technical Steps, Current Status, and Review of Outcomes.

    Subramaniam, Ramnath

    Frontiers in pediatrics

    2019  Volume 7, Page(s) 1

    Abstract: Purpose: ...

    Abstract Purpose:
    Language English
    Publishing date 2019-01-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2711999-3
    ISSN 2296-2360
    ISSN 2296-2360
    DOI 10.3389/fped.2019.00001
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  5. Article ; Online: Experience with detrusorotomy in children by open and robotic approach.

    Subramaniam, Ramnath

    World journal of urology

    2019  Volume 38, Issue 8, Page(s) 1869–1874

    Abstract: Purpose: To present the author's experience with detrusorotomy (DM) for refractory detrusor overactivity (RDO) with open and robotic approach.: Methods: Children who underwent DM for RDO in a single surgeon series since 2012 were identified from a ... ...

    Abstract Purpose: To present the author's experience with detrusorotomy (DM) for refractory detrusor overactivity (RDO) with open and robotic approach.
    Methods: Children who underwent DM for RDO in a single surgeon series since 2012 were identified from a prospectively maintained database. Those who completed the defined strict bladder cycling regime postoperatively were included in this report excluding those who failed the bladder cycling regime.
    Results: Ten children (M7: F3) were included in this report, six open and four robotic. All procedures were completed with no conversion to open in the robotic group. There were no intra or postoperative complications from the procedures. Duration of procedure was lower in robotic group (125 min) vs the open group at (208 min). Hospital stay was also lower in the robotic group (2.7 days) compared to the open group (5.6 days). All children in open group had concomitant Mitrofanoff channel created for bladder drainage. One child in robotic group had concomitant Mitrofanoff channel during DM. Median follow-up is longer at 54 months (31-82) in open group compared to 14 months (5-21). All children are clinically well with safe upper tracts on US scan in both series. Estimated % change in bladder capacity is similar in both groups at 140 (90-200) and 126 (80-200) for open and robotic groups, respectively.
    Conclusions: DM as an extension of medical treatment for RDO can be performed safely and is effective in children. Preliminary experience with robotic approach to DM is promising with reduced duration of procedure and hospital stay.
    MeSH term(s) Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Male ; Prospective Studies ; Robotic Surgical Procedures ; Urinary Bladder/surgery ; Urinary Bladder, Overactive/surgery ; Urologic Surgical Procedures/methods
    Language English
    Publishing date 2019-05-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 380333-8
    ISSN 1433-8726 ; 0724-4983
    ISSN (online) 1433-8726
    ISSN 0724-4983
    DOI 10.1007/s00345-019-02777-9
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  6. Article: Anaesthetic concerns in preterm and term neonates.

    Subramaniam, Rajeshwari

    Indian journal of anaesthesia

    2019  Volume 63, Issue 9, Page(s) 771–779

    Abstract: Anaesthesia for neonates is a composite of good knowledge of neonatal and transitional physiology combined with skill in airway maintenance and vascular access. When the newborn is a preterm, the complexities of management increase due to the small size ... ...

    Abstract Anaesthesia for neonates is a composite of good knowledge of neonatal and transitional physiology combined with skill in airway maintenance and vascular access. When the newborn is a preterm, the complexities of management increase due to the small size and accompanying issues such as bronchopulmonary dysplasia and apnoea. World over, the number of survivors of preterm birth is on the increase. We searched Pubmed for "Anesthesia, apnea, neonatal, neonates, physiology, preterm, spinal anesthesia", as well as cross references from review articles. These babies have a high incidence of conditions warranting surgery (e.g., tracheoesophaeal fistula, congenital diaphragmatic hernia, anorectal malformations, incarcerated hernia, necrotising enterocolitis). The possibility of neurodevelopmental harm by anaesthetics is currently the topic of active research. In parallel, advances in paediatric anaesthesia equipment, use of regional and neuraxial anaesthesia and availability of monitoring have steadily increased the safety of anaesthesia in these tiny patients.
    Language English
    Publishing date 2019-03-15
    Publishing country India
    Document type Journal Article ; Review
    ZDB-ID 412570-8
    ISSN 0019-5049
    ISSN 0019-5049
    DOI 10.4103/ija.IJA_591_19
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  7. Article ; Online: Duplex systems: Top-down or bottom-up approach?

    Keene, D J B / Subramaniam, R

    Journal of pediatric urology

    2020  Volume 16, Issue 3, Page(s) 387.e1–387.e8

    Abstract: Introduction: Duplex systems can be complicated by reflux, ureterocele, obstruction (most commonly PUJ in a lower moiety) and wetting secondary to an ectopic ureteric insertion in girls. The decision making algorithm for selection of surgical approach ... ...

    Abstract Introduction: Duplex systems can be complicated by reflux, ureterocele, obstruction (most commonly PUJ in a lower moiety) and wetting secondary to an ectopic ureteric insertion in girls. The decision making algorithm for selection of surgical approach is complex and there is no consensus. The authors described the outcomes following an upper urinary tract approach in 2011(1) and now compare these results in a similar group of patients managed using a lower approach.
    Objectives: To assess whether a top-down or bottom-up approach results in different likelihoods for further surgery.
    Study design: A prospectively database was maintained for consecutive patients undergoing surgery for duplex systems by a single surgeon between 2003 and 2015. Patients were classified into 2 groups; Group 1 initial intention for upper urinary tract approach (heminephroureterectomy-HN) or Group 2 lower urinary tract approach (bladder reconstructive surgery-BRS). The requirement for further surgery was recorded-endoscopic incision (EI), bladder reconstructive surgery (BRS), endoscopic correction of reflux (ECR), heminephroureterectomy (HN). Indications for initial and subsequent surgery included urinary tract infection, VUJ obstruction and incontinence. Endoscopic incision was not performed for patients with an asymptomatic ureterocele. Statistical analysis consisted of Fisher's exact test with a 2 tail p value < 0.05 being statistically significant.
    Results: 79 patients underwent surgery for duplex systems. 39 patients had HN initially (Group 1) and 40 patients had BRS initially (Group 2). Further surgery was performed in 21% of patients from Group 1 (8 BRS) vs 5% of patients from Group 2 (1 redo BRS, 1 ECR). Significantly less additional surgical procedures were performed after BRS compared to HN (p = 0.048). The presence of both reflux and ureterocele increases the chances of further surgery in those patients who had HN initially compared to BRS (p = 0.01). No patients developed urinary retention or required intermittent catheterisation to improve bladder emptying.
    Conclusions: Bladder reconstructive surgery (BRS) reduces the requirement for further surgery compared to heminephroureterectomy (HN) in symptomatic patients with a duplex kidney and either dilating vesicoureteric reflux or ureterocele.
    MeSH term(s) Female ; Humans ; Infant ; Kidney Diseases ; Retrospective Studies ; Ureterocele ; Urologic Surgical Procedures ; Vesico-Ureteral Reflux
    Language English
    Publishing date 2020-03-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 2237683-5
    ISSN 1873-4898 ; 1477-5131
    ISSN (online) 1873-4898
    ISSN 1477-5131
    DOI 10.1016/j.jpurol.2020.03.005
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  8. Article ; Online: Response to 'Duplex systems: Top down or bottom-up approach?

    Keene, D J B / Subramaniam, R

    Journal of pediatric urology

    2020  Volume 17, Issue 1, Page(s) 136–138

    MeSH term(s) Humans ; Ureter ; Ureterocele
    Language English
    Publishing date 2020-12-19
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 2237683-5
    ISSN 1873-4898 ; 1477-5131
    ISSN (online) 1873-4898
    ISSN 1477-5131
    DOI 10.1016/j.jpurol.2020.11.046
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  9. Article ; Online: Current Use of and Indications for Robot-assisted Surgery in Paediatric Urology.

    Subramaniam, Ramnath

    European urology focus

    2018  Volume 4, Issue 5, Page(s) 662–664

    Abstract: The use of robot-assisted surgery (RAS) by paediatric urologists is increasing. This mini review looks at the current status of RAS in paediatric urology. The challenges involved in RAS use in children are reviewed, as well as the indications for and ... ...

    Abstract The use of robot-assisted surgery (RAS) by paediatric urologists is increasing. This mini review looks at the current status of RAS in paediatric urology. The challenges involved in RAS use in children are reviewed, as well as the indications for and feasibility of procedures performed and, where possible, outcomes with RAS. PATIENT SUMMARY: The current status of robot-assisted surgery (RAS) in paediatric urology, together with the challenges in using RAS for children is outlined in this mini review. Innovative adaptation has pushed the boundaries as regards the feasibility of RAS procedures in children with good outcomes.
    MeSH term(s) Analgesia/statistics & numerical data ; Child ; Humans ; Laparoscopy/methods ; Length of Stay ; Meta-Analysis as Topic ; Outcome Assessment (Health Care) ; Robotic Surgical Procedures/standards ; Urologic Surgical Procedures/instrumentation ; Urologic Surgical Procedures/statistics & numerical data ; Urology/instrumentation ; Urology/statistics & numerical data
    Language English
    Publishing date 2018-09-05
    Publishing country Netherlands
    Document type Comparative Study ; Journal Article ; Review
    ISSN 2405-4569
    ISSN (online) 2405-4569
    DOI 10.1016/j.euf.2018.08.020
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  10. Article: Atypical Presentation of Herpes Stromal Keratitis in a Contact Lens Wearer.

    Subramaniam, Ruknesvary / Sonny Teo, Khairy Shamel / Muhammed, Julieana

    Cureus

    2023  Volume 15, Issue 5, Page(s) e38438

    Abstract: This article describes the case of a 21-year-old female habitual contact lens wearer who complained of left eye pain, redness, and decreased vision for one week. When a ring-shaped corneal infiltration indicative of an Acanthamoeba infection was ... ...

    Abstract This article describes the case of a 21-year-old female habitual contact lens wearer who complained of left eye pain, redness, and decreased vision for one week. When a ring-shaped corneal infiltration indicative of an Acanthamoeba infection was discovered, standard anti-amoebic topical therapy with polyhexamethylene biguanide and chlorhexidine was commenced. However, her keratitis worsened. At the same time, corneal scraping revealed no pathogens. An anterior chamber examination revealed a loss of corneal sensation, and a positive herpes simplex virus (HSV) immunoglobulin G serology test indicated HSV keratitis. She was eventually treated with oral anti-viral medication and recovered completely. Her case was unusual, as she had a history of contact lens use, painful corneal ulceration, and the development of Acanthamoeba keratitis-like corneal ring infiltration. This case also reinforces the various manifestations of HSV keratitis, which lead to delayed diagnosis and treatment.
    Language English
    Publishing date 2023-05-02
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.38438
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