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Article ; Online: Proteome profiling of nasopharynx reveals pathophysiological signature of COVID-19 disease severity

Ooi, Amanda / Esau, Luke E. / Pugachev, Artyom / Groen, Arnoud / Mfarrej, Sara / Salunke, Rahul P. / Subudhi, Amit K. / Ben-Rached, Fathia / Alofi, Fadwa / Alsomali, Afrah / Alquthami, Khaled / Khogeer, Asim / Hashem, Anwar M. / Almontashiri, Naif / Magistretti, Pierre J. / Hala, Sharif / Pain, Arnab

bioRxiv

Abstract: An aberrant innate immune system caused by the beta coronavirus SARS-CoV-2 is a characteristic manifestation of severe coronavirus disease 2019 (COVID-19). Here, we performed proteome profiling of nasopharyngeal (NP) swabs from 273 hospitalized patients ... ...

Abstract	An aberrant innate immune system caused by the beta coronavirus SARS-CoV-2 is a characteristic manifestation of severe coronavirus disease 2019 (COVID-19). Here, we performed proteome profiling of nasopharyngeal (NP) swabs from 273 hospitalized patients with mild and severe COVID-19 symptoms, including non-survivors. We identified depletion in STAT1-mediated type I interferon response, retinol metabolism and NRF2 antioxidant system that are associated with disease severity in our patient demography. We found that the dysregulation of glucocorticoid signaling and renin-angiotensin-aldosterone system (RAAS) contribute to the pathophysiology of COVID-19 fatality. Hyperactivation of host innate immune system was observed in severe patients, marked by elevated proteins involved in neutrophil degranulation and platelet aggregation. Our study using high-throughput proteomics on the nasopharynx of COVID-19 patients provides additional evidence on the SARS-CoV-2-induced pathophysiological signatures of disease severity and fatality.
Keywords	covid19
Language	English
Publishing date	2023-07-10
Publisher	Cold Spring Harbor Laboratory
Document type	Article ; Online
DOI	10.1101/2023.07.09.548285
Database	COVID19

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Article ; Online: Malaria parasites regulate intra-erythrocytic development duration via serpentine receptor 10 to coordinate with host rhythms.

Subudhi, Amit K / O'Donnell, Aidan J / Ramaprasad, Abhinay / Abkallo, Hussein M / Kaushik, Abhinav / Ansari, Hifzur R / Abdel-Haleem, Alyaa M / Ben Rached, Fathia / Kaneko, Osamu / Culleton, Richard / Reece, Sarah E / Pain, Arnab

Nature communications

2020 Volume 11, Issue 1, Page(s) 2763

Abstract: Malaria parasites complete their intra-erythrocytic developmental cycle (IDC) in multiples of 24 h suggesting a circadian basis, but the mechanism controlling this periodicity is unknown. Combining in vivo and in vitro approaches utilizing rodent and ... ...

Abstract	Malaria parasites complete their intra-erythrocytic developmental cycle (IDC) in multiples of 24 h suggesting a circadian basis, but the mechanism controlling this periodicity is unknown. Combining in vivo and in vitro approaches utilizing rodent and human malaria parasites, we reveal that: (i) 57% of Plasmodium chabaudi genes exhibit daily rhythms in transcription; (ii) 58% of these genes lose transcriptional rhythmicity when the IDC is out-of-synchrony with host rhythms; (iii) 6% of Plasmodium falciparum genes show 24 h rhythms in expression under free-running conditions; (iv) Serpentine receptor 10 (SR10) has a 24 h transcriptional rhythm and disrupting it in rodent malaria parasites shortens the IDC by 2-3 h; (v) Multiple processes including DNA replication, and the ubiquitin and proteasome pathways, are affected by loss of coordination with host rhythms and by disruption of SR10. Our results reveal malaria parasites are at least partly responsible for scheduling the IDC and coordinating their development with host daily rhythms.
MeSH term(s)	Animals ; Caenorhabditis elegans Proteins ; Circadian Rhythm/physiology ; Disease Models, Animal ; Erythropoiesis/physiology ; Female ; Gene Expression ; Host-Parasite Interactions/genetics ; Host-Parasite Interactions/physiology ; Humans ; Malaria/metabolism ; Malaria/parasitology ; Mice ; Mice, Knockout ; Plasmodium chabaudi/genetics ; Plasmodium chabaudi/growth & development ; Plasmodium falciparum/genetics ; Plasmodium falciparum/growth & development ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism ; Rodentia ; Secologanin Tryptamine Alkaloids/metabolism ; Transcriptome
Chemical Substances	Caenorhabditis elegans Proteins ; Protozoan Proteins ; Receptors, G-Protein-Coupled ; SRD-1 protein, C elegans ; Secologanin Tryptamine Alkaloids ; serpentine (alkaloid) (B503RKE34F)
Language	English
Publishing date	2020-06-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-020-16593-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2

Ali, Zahir / Aman, Rashid / Mahas, Ahmed / Rao, Gundra Sivakrishna / Tehseen, Muhammad / Marsic, Tin / Salunke, Rahul / Subudhi, Amit K / Hala, Sharif M / Hamdan, Samir M / Pain, Arnab / Alofi, Fadwa S / Alsomali, Afrah / Hashem, Anwar M / Khogeer, Asim / Almontashiri, Naif A.M / Abedalthagafi, Malak / Hassan, Norhan / Mahfouz, Magdy M

Virus research. 2020 Oct. 15, v. 288

2020

Abstract: The COVID-19 pandemic caused by SARS-CoV-2 affects all aspects of human life. Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly are urgently needed to manage and control the spread of SARS-CoV-2. RT-qPCR ... ...

Abstract	The COVID-19 pandemic caused by SARS-CoV-2 affects all aspects of human life. Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly are urgently needed to manage and control the spread of SARS-CoV-2. RT-qPCR based methods are the gold standard for SARS-CoV-2 detection. However, these methods require trained personnel, sophisticated infrastructure, and a long turnaround time, thereby limiting their usefulness. Reverse transcription-loop-mediated isothermal amplification (RT-LAMP), a one-step nucleic acid amplification method conducted at a single temperature, has been used for colorimetric virus detection. CRISPR-Cas12 and CRISPR-Cas13 systems, which possess collateral activity against ssDNA and RNA, respectively, have also been harnessed for virus detection. Here, we built an efficient, rapid, specific, sensitive, user-friendly SARS-CoV-2 detection module that combines the robust virus amplification of RT-LAMP with the specific detection ability of SARS-CoV-2 by CRISPR-Cas12. Furthermore, we combined the RT-LAMP-CRISPR-Cas12 module with lateral flow cells to enable highly efficient point-of-care SARS-CoV-2 detection. Our iSCAN SARS-CoV-2 detection module, which exhibits the critical features of a robust molecular diagnostic device, should facilitate the effective management and control of COVID-19.
Keywords	COVID-19 infection ; RNA ; Severe acute respiratory syndrome coronavirus 2 ; colorimetry ; diagnostic techniques ; gene amplification ; human resources ; humans ; infrastructure ; point-of-care systems ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; reverse transcription loop-mediated isothermal amplification ; single-stranded DNA ; temperature ; viruses
Language	English
Dates of publication	2020-1015
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	605780-9
ISSN	1872-7492 ; 0168-1702
ISSN (online)	1872-7492
ISSN	0168-1702
DOI	10.1016/j.virusres.2020.198129
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Accelerating Early Antituberculosis Drug Discovery by Creating Mycobacterial Indicator Strains That Predict Mode of Action.

Boot, Maikel / Commandeur, Susanna / Subudhi, Amit K / Bahira, Meriem / Smith, Trever C / Abdallah, Abdallah M / van Gemert, Mae / Lelièvre, Joël / Ballell, Lluís / Aldridge, Bree B / Pain, Arnab / Speer, Alexander / Bitter, Wilbert

Antimicrobial agents and chemotherapy

2018 Volume 62, Issue 7

Abstract: Due to the rise of drug-resistant forms of tuberculosis, there is an urgent need for novel antibiotics to effectively combat these cases and shorten treatment regimens. Recently, drug screens using whole-cell analyses have been shown to be successful. ... ...

Abstract	Due to the rise of drug-resistant forms of tuberculosis, there is an urgent need for novel antibiotics to effectively combat these cases and shorten treatment regimens. Recently, drug screens using whole-cell analyses have been shown to be successful. However, current high-throughput screens focus mostly on
MeSH term(s)	Animals ; Antitubercular Agents/pharmacology ; Base Sequence ; Cell Line ; Ciprofloxacin/pharmacology ; Drug Discovery/methods ; Ethambutol/pharmacology ; Humans ; Isoniazid/pharmacology ; Macrophages/drug effects ; Mice ; Mycobacterium marinum/drug effects ; Mycobacterium marinum/genetics ; Mycobacterium tuberculosis/drug effects ; Mycobacterium tuberculosis/genetics ; RAW 264.7 Cells ; RNA, Bacterial/genetics ; Rifampin/pharmacology ; Sequence Analysis, RNA ; Streptomycin/pharmacology ; Transcription, Genetic/drug effects ; Transcription, Genetic/genetics ; Tuberculosis, Pulmonary/drug therapy ; Tuberculosis, Pulmonary/microbiology
Chemical Substances	Antitubercular Agents ; RNA, Bacterial ; Ciprofloxacin (5E8K9I0O4U) ; Ethambutol (8G167061QZ) ; Isoniazid (V83O1VOZ8L) ; Rifampin (VJT6J7R4TR) ; Streptomycin (Y45QSO73OB)
Language	English
Publishing date	2018-06-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	217602-6
ISSN	1098-6596 ; 0066-4804
ISSN (online)	1098-6596
ISSN	0066-4804
DOI	10.1128/AAC.00083-18
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Article ; Online: Creation and preclinical evaluation of genetically attenuated malaria parasites arresting growth late in the liver.

Franke-Fayard, Blandine / Marin-Mogollon, Catherin / Geurten, Fiona J A / Chevalley-Maurel, Séverine / Ramesar, Jai / Kroeze, Hans / Baalbergen, Els / Wessels, Els / Baron, Ludivine / Soulard, Valérie / Martinson, Thomas / Aleshnick, Maya / Huijs, Antonius T G / Subudhi, Amit K / Miyazaki, Yukiko / Othman, Ahmad Syibli / Kolli, Surendra Kumar / Lamers, Olivia A C / Roques, Magali /

Stanway, Rebecca R / Murphy, Sean C / Foquet, Lander / Moita, Diana / Mendes, António M / Prudêncio, Miguel / Dechering, Koen J / Heussler, Volker T / Pain, Arnab / Wilder, Brandon K / Roestenberg, Meta / Janse, Chris J

NPJ vaccines

2022 Volume 7, Issue 1, Page(s) 139

Abstract: Whole-sporozoite (WSp) malaria vaccines induce protective immune responses in animal malaria models and in humans. A recent clinical trial with a WSp vaccine comprising genetically attenuated parasites (GAP) which arrest growth early in the liver (PfSPZ- ... ...

Abstract	Whole-sporozoite (WSp) malaria vaccines induce protective immune responses in animal malaria models and in humans. A recent clinical trial with a WSp vaccine comprising genetically attenuated parasites (GAP) which arrest growth early in the liver (PfSPZ-GA1), showed that GAPs can be safely administered to humans and immunogenicity is comparable to radiation-attenuated PfSPZ Vaccine. GAPs that arrest late in the liver stage (LA-GAP) have potential for increased potency as shown in rodent malaria models. Here we describe the generation of four putative P. falciparum LA-GAPs, generated by CRISPR/Cas9-mediated gene deletion. One out of four gene-deletion mutants produced sporozoites in sufficient numbers for further preclinical evaluation. This mutant, PfΔmei2, lacking the mei2-like RNA gene, showed late liver growth arrest in human liver-chimeric mice with human erythrocytes, absence of unwanted genetic alterations and sensitivity to antimalarial drugs. These features of PfΔmei2 make it a promising vaccine candidate, supporting further clinical evaluation. PfΔmei2 (GA2) has passed regulatory approval for safety and efficacy testing in humans based on the findings reported in this study.
Language	English
Publishing date	2022-11-04
Publishing country	England
Document type	Journal Article
ISSN	2059-0105
ISSN (online)	2059-0105
DOI	10.1038/s41541-022-00558-x
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Article ; Online: iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2.

Ali, Zahir / Aman, Rashid / Mahas, Ahmed / Rao, Gundra Sivakrishna / Tehseen, Muhammad / Marsic, Tin / Salunke, Rahul / Subudhi, Amit K / Hala, Sharif M / Hamdan, Samir M / Pain, Arnab / Alofi, Fadwa S / Alsomali, Afrah / Hashem, Anwar M / Khogeer, Asim / Almontashiri, Naif A M / Abedalthagafi, Malak / Hassan, Norhan / Mahfouz, Magdy M

Virus research

2020 Volume 288, Page(s) 198129

Abstract	The COVID-19 pandemic caused by SARS-CoV-2 affects all aspects of human life. Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly are urgently needed to manage and control the spread of SARS-CoV-2. RT-qPCR based methods are the gold standard for SARS-CoV-2 detection. However, these methods require trained personnel, sophisticated infrastructure, and a long turnaround time, thereby limiting their usefulness. Reverse transcription-loop-mediated isothermal amplification (RT-LAMP), a one-step nucleic acid amplification method conducted at a single temperature, has been used for colorimetric virus detection. CRISPR-Cas12 and CRISPR-Cas13 systems, which possess collateral activity against ssDNA and RNA, respectively, have also been harnessed for virus detection. Here, we built an efficient, rapid, specific, sensitive, user-friendly SARS-CoV-2 detection module that combines the robust virus amplification of RT-LAMP with the specific detection ability of SARS-CoV-2 by CRISPR-Cas12. Furthermore, we combined the RT-LAMP-CRISPR-Cas12 module with lateral flow cells to enable highly efficient point-of-care SARS-CoV-2 detection. Our iSCAN SARS-CoV-2 detection module, which exhibits the critical features of a robust molecular diagnostic device, should facilitate the effective management and control of COVID-19.
MeSH term(s)	Betacoronavirus/genetics ; COVID-19 ; COVID-19 Testing ; CRISPR-Cas Systems ; Clinical Laboratory Techniques/instrumentation ; Clinical Laboratory Techniques/methods ; Colorimetry/instrumentation ; Colorimetry/methods ; Coronavirus Infections/diagnosis ; Coronavirus Infections/virology ; Endodeoxyribonucleases/chemistry ; Humans ; Molecular Diagnostic Techniques/instrumentation ; Molecular Diagnostic Techniques/methods ; Nucleic Acid Amplification Techniques/instrumentation ; Nucleic Acid Amplification Techniques/methods ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/virology ; Point-of-Care Systems ; Rheology ; SARS-CoV-2 ; Sensitivity and Specificity
Chemical Substances	Endodeoxyribonucleases (EC 3.1.-)
Keywords	covid19
Language	English
Publishing date	2020-08-18
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605780-9
ISSN	1872-7492 ; 0168-1702
ISSN (online)	1872-7492
ISSN	0168-1702
DOI	10.1016/j.virusres.2020.198129
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Article: iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2

Ali, Zahir / Aman, Rashid / Mahas, Ahmed / Rao, Gundra Sivakrishna / Tehseen, Muhammad / Marsic, Tin / Salunke, Rahul / Subudhi, Amit K / Hala, Sharif M / Hamdan, Samir M / Pain, Arnab / Alofi, Fadwa S / Alsomali, Afrah / Hashem, Anwar M / Khogeer, Asim / Almontashiri, Naif A M / Abedalthagafi, Malak / Hassan, Norhan / Mahfouz, Magdy M

Virus Res

Abstract	The COVID-19 pandemic caused by SARS-CoV-2 affects all aspects of human life. Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly are urgently needed to manage and control the spread of SARS-CoV-2. RT-qPCR based methods are the gold standard for SARS-CoV-2 detection. However, these methods require trained personnel, sophisticated infrastructure, and a long turnaround time, thereby limiting their usefulness. Reverse transcription-loop-mediated isothermal amplification (RT-LAMP), a one-step nucleic acid amplification method conducted at a single temperature, has been used for colorimetric virus detection. CRISPR-Cas12 and CRISPR-Cas13 systems, which possess collateral activity against ssDNA and RNA, respectively, have also been harnessed for virus detection. Here, we built an efficient, rapid, specific, sensitive, user-friendly SARS-CoV-2 detection module that combines the robust virus amplification of RT-LAMP with the specific detection ability of SARS-CoV-2 by CRISPR-Cas12. Furthermore, we combined the RT-LAMP-CRISPR-Cas12 module with lateral flow cells to enable highly efficient point-of-care SARS-CoV-2 detection. Our iSCAN SARS-CoV-2 detection module, which exhibits the critical features of a robust molecular diagnostic device, should facilitate the effective management and control of COVID-19.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #719033
Database	COVID19

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Article ; Online: iSCAN

Ali, Zahir / Aman, Rashid / Mahas, Ahmed / Rao, Gundra Sivakrishna / Tehseen, Muhammad / Marsic, Tin / Salunke, Rahul / Subudhi, Amit K. / Hala, Sharif M. / Hamdan, Samir M. / Pain, Arnab / Alofi, Fadwa S. / Alsomali, Afrah / Hashem, Anwar M. / Khogeer, Asim / Almontashiri, Naif A.M. / Abedalthagafi, Malak / Hassan, Norhan / Mahfouz, Magdy M.

Virus Research

An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2

2020 Volume 288, Page(s) 198129

Keywords	Cancer Research ; Virology ; Infectious Diseases ; covid19
Language	English
Publisher	Elsevier BV
Publishing country	us
Document type	Article ; Online
ZDB-ID	605780-9
ISSN	1872-7492 ; 0168-1702
ISSN (online)	1872-7492
ISSN	0168-1702
DOI	10.1016/j.virusres.2020.198129
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Development and evaluation of a 28S rRNA gene-based nested PCR assay for P. falciparum and P. vivax.

Pakalapati, Deepak / Garg, Shilpi / Middha, Sheetal / Acharya, Jyoti / Subudhi, Amit K / Boopathi, Arunachalam P / Saxena, Vishal / Kochar, Sanjay K / Kochar, Dhanpat K / Das, Ashis

Pathogens and global health

2013 Volume 107, Issue 4, Page(s) 180–188

Abstract: The 28S rRNA gene was amplified and sequenced from P. falciparum and P. vivax isolates collected from northwest India. Based upon the sequence diversity of the Plasmodium 28SrRNA gene in comparison with its human counterpart, various nested polymerase ... ...

Abstract	The 28S rRNA gene was amplified and sequenced from P. falciparum and P. vivax isolates collected from northwest India. Based upon the sequence diversity of the Plasmodium 28SrRNA gene in comparison with its human counterpart, various nested polymerase chain reaction (PCR) primers were designed from the 3R region of the 28SrRNA gene and evaluated on field isolates. This is the first report demonstrating the utility of this gene for species-specific diagnosis of malaria for these two species, prevalent in India. The initial evaluation on 363 clinical isolates indicated that, in comparison with microscopy, which showed sensitivity and specificity of 85·39% and 100% respectively, the sensitivity and specificity of the nested PCR assay was found to be 99·08% and 100% respectively. This assay was also successful in detecting mixed infections that are undetected by microscopy. Our results demonstrate the utility of the 28S rRNA gene as a diagnostic target for the detection of the major plasmodial species infecting humans.
MeSH term(s)	Coinfection/diagnosis ; Coinfection/parasitology ; DNA Primers/genetics ; DNA, Protozoan/chemistry ; DNA, Protozoan/genetics ; Genes, rRNA ; Humans ; India ; Malaria/diagnosis ; Malaria/parasitology ; Molecular Diagnostic Techniques/methods ; Parasitology/methods ; Plasmodium falciparum/classification ; Plasmodium falciparum/genetics ; Plasmodium vivax/classification ; Plasmodium vivax/genetics ; Polymerase Chain Reaction/methods ; RNA, Ribosomal, 28S/genetics ; Sensitivity and Specificity ; Sequence Analysis, DNA
Chemical Substances	DNA Primers ; DNA, Protozoan ; RNA, Ribosomal, 28S
Language	English
Publishing date	2013-06-29
Publishing country	England
Document type	Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2625162-0
ISSN	2047-7732 ; 2047-7724
ISSN (online)	2047-7732
ISSN	2047-7724
DOI	10.1179/2047773213Y.0000000090
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Article: Plasmodium vivax apicoplast genome: A comparative analysis of major genes from Indian field isolates

Saxena, Vishal / Garg, Shilpi / Tripathi, Jyotsna / Sharma, Sonal / Pakalapati, Deepak / Subudhi, Amit K / Boopathi, P.A / Saggu, Gagandeep S / Kochar, Sanjay K / Kochar, Dhanpat K / Das, Ashis

Acta tropica. 2012 Apr., v. 122, no. 1

2012

Abstract: The apicomplexan parasite Plasmodium vivax is responsible for causing more than 70% of human malaria cases in Central and South America, Southeastern Asia and the Indian subcontinent. The rising severity of the disease and the increasing incidences of ... ...

Abstract	The apicomplexan parasite Plasmodium vivax is responsible for causing more than 70% of human malaria cases in Central and South America, Southeastern Asia and the Indian subcontinent. The rising severity of the disease and the increasing incidences of resistance shown by this parasite towards usual therapeutic regimens have necessitated investigation of putative novel drug targets to combat this disease. The apicoplast, an organelle of procaryotic origin, and its circular genome carrying genes of possible functional importance, are being looked upon as potential drug targets. The genes on this circular genome are believed to be highly conserved among all Plasmodium species. Till date, the plastid genome of P. falciparum, P. berghei and P. chabaudi have been detailed while partial sequences of some genes from other parasites including P. vivax have been studied for identifying evolutionary positions of these parasites. The functional aspects and significance of most of these genes are still hypothetical. In one of our previous reports, we have detailed the complete sequence, as well as structural and functional characteristics of the Elongation factor encoding tufA gene from the plastid genome of P. vivax. We present here the sequences of large and small subunit rRNA (lsu and ssu rRNA) genes, sufB (ORF470) gene, RNA polymerase (rpo B, C) subunit genes and clpC (casienolytic protease) gene from the plastid genome of P. vivax. A comparative analysis of these genes between P. vivax and P. falciparum reveals approximately 5–16% differences. A codon usage analysis of major plastid genes has shown a high frequency of codons rich in A/T at any or all of the three positions in all the species. TTA, AAT, AAA, TAT, and ATA are the major preferred codons. The sequences, functional domains and structural analysis of respective proteins do not show any variations in the active sites. A comparative analysis of these Indian P. vivax plastid genome encoded genes has also been done to understand the evolutionary position of the Indian parasite in comparison to other Plasmodium species.
Keywords	DNA-directed RNA polymerase ; Plasmodium vivax ; active sites ; codons ; correlation ; disease severity ; drugs ; functional properties ; humans ; major genes ; malaria ; parasites ; plastid DNA ; proteinases ; proteins ; ribosomal RNA ; Asia ; South America
Language	English
Dates of publication	2012-04
Size	p. 138-149.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	210415-5
ISSN	1873-6254 ; 0001-706X
ISSN (online)	1873-6254
ISSN	0001-706X
DOI	10.1016/j.actatropica.2012.01.007
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