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  1. Article ; Online: Laparoscopic cholecystectomy in double gallbladder with dual pathology

    Sumanta Kumar Ghosh

    Journal of Minimal Access Surgery, Vol 10, Iss 2, Pp 93-

    2014  Volume 96

    Abstract: Double gallbladder is a rare embryological anomaly of clinical significance. Despite availability of modern imaging, only 50% of recently reported cases had preoperative diagnosis, which is desirable in every case to avoid serious operative complications. ...

    Abstract Double gallbladder is a rare embryological anomaly of clinical significance. Despite availability of modern imaging, only 50% of recently reported cases had preoperative diagnosis, which is desirable in every case to avoid serious operative complications. Double pathology in double gallbladder is extremely rare with only 3 reporting′s available till date to the best of author′s knowledge. With a preoperative diagnosis of double gallbladder, laparoscopic cholecystectomy can be safely and successfully performed with meticulous dissection, aided by operative cholangiogram. However in all such attempts a lower threshold should be kept for conversion to open surgery. Awareness about this anomaly amongst radiologists and surgeons is of crucial importance. Double gallbladder does not present with any specific symptom, neither it increases disease possibility in either lobe. Prophylactic cholecystectomy has no role in asymptomatic cases diagnosed accidentally. Author reports a case of a symptomatic young male with double gallbladder who presented with short history of dyspepsia, abdominal pain and fever. Definite preoperative diagnosis was reached with ultrasound scan and magnetic resonance cholangio pancreatography and subsequently dealt with laparoscopically. Calculous cholecystitis affected one lobe and acalculous empyema the other. While the 1st lobe drained though a cystic duct into common bile duct (CBD), the 2nd was without any communication with either CBD or its counterpart, thus remained as a blind vesicle.
    Keywords Double ; gallbladder anomaly ; laparoscopic cholecystectomy ; pathology ; Surgery ; RD1-811 ; Medicine ; R ; Diseases of the genitourinary system. Urology ; RC870-923 ; Specialties of internal medicine ; RC581-951 ; Internal medicine ; RC31-1245
    Subject code 610
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher Medknow Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: One Stage Emergency Pancreatoduodenectomy for Isolated Injury to Pancreatic Head Following Blunt Abdominal Trauma

    Sumanta Kumar Ghosh

    Bulletin of Emergency And Trauma, Vol 1, Iss 3, Pp 127-

    Case Report and Review of Literature

    2013  Volume 129

    Abstract: Major pancreatic injury following blunt abdominal trauma by itself is a relatively rare occurrence, and in vast majority of cases (95%) it is associated with injury to adjacent major vessels and organs; thus making isolated major pancreatic injury even ... ...

    Abstract Major pancreatic injury following blunt abdominal trauma by itself is a relatively rare occurrence, and in vast majority of cases (95%) it is associated with injury to adjacent major vessels and organs; thus making isolated major pancreatic injury even rarer. While most pancreatic injuries are managed by simple measures like debridement and drainage, complex proximal injury poses surgical challenge regarding surgical skill and judgement. Disproportionate approach at any stage of management can contribute to high mortality and morbidity. Emergency pancreatoduodenectomy plays a limited but important role in managing serious trauma to proximal pancreas and duodenum. Author presents a case where isolated injury to head of pancreas required emergency pancreatoduodenectomy. After a bizarre road accident, a middle aged male underwent emergency laparotomy for intraperitoneal bleeding and during exploration a deep transverse laceration with ampullary disruption was found in the head of the organ. Duodenum in all its part was intact and there was no other injury. The nature and site of injury made emergency pancreatoduodenectomy the only viable option. Leaking pancreatojejunostomy enhances infective complications that lead to late mortality. To circumvent this problem there is enthusiasm for staged surgery with resection and tube pancreatostomy in first stage, leaving the difficult anastomosis for a later date, However, if the patient is haemodynamically stable and operated reasonably early, one stage pancreatoduodenectomy gives good result and avoids repeating surgery with inherent problems and reduces hospital stay. For successful management of pancreatic trauma it is essential to make early diagnosis of duct disruption, with sound application of operative skill and judgement by treating surgeon.
    Keywords Isolated pancreatic injury ; Emergency pancreatoduodenectomy ; Major duct disruption ; Staged surgery ; Associ ated injury ; Surgery ; RD1-811 ; Medicine ; R ; DOAJ:Surgery ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Subject code 610
    Language English
    Publishing date 2013-07-01T00:00:00Z
    Publisher Shiraz University of Medical Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: An experimental modeling of trinomial bioengineering- crp, rDNA, and transporter engineering within single cell factory for maximizing two-phase bioreduction

    Basak, Souvik / Ashish N. Aphale / Nanda Gopal Sahoo / Prabir K. Patra / Sumanta Kumar Ghosh / Vinay Deep Punetha

    International journal of biological macromolecules. 2017 Feb., v. 95

    2017  

    Abstract: A carbonyl reductase (cr) gene from Candida glabrata CBS138 has been heterologously expressed in cofactor regenerating E. coli host to convert Ethyl-4-chloro-3-oxobutanoate (COBE) into Ethyl-4-chloro-3-hydroxybutanoate (CHBE). The CR enzyme exhibited ... ...

    Abstract A carbonyl reductase (cr) gene from Candida glabrata CBS138 has been heterologously expressed in cofactor regenerating E. coli host to convert Ethyl-4-chloro-3-oxobutanoate (COBE) into Ethyl-4-chloro-3-hydroxybutanoate (CHBE). The CR enzyme exhibited marked velocity at substrate concentration as high as 363mM with highest turnover number (112.77±3.95s−1). Solitary recombineering of such catalytic cell reproduced CHBE 161.04g/L per g of dry cell weight (DCW). Introduction of combinatorially engineered crp (crp*, F136I) into this heterologous E. coli host yielded CHBE 477.54g/L/gDCW. Furthermore, using nerolidol as exogenous cell transporter, the CHBE productivity has been towered to 710.88g/L/gDCW. The CHBE production has thus been upscaled to 8–12 times than those reported so far. qRT-PCR studies revealed that both membrane efflux channels such as acrAB as well as ROS scavenger genes such as ahpCF have been activated by engineering crp. Moreover, membrane protecting genes such as manXYZ together with solvent extrusion associated genes such as glpC have been upregulated inside mutant host. Although numerous proteins have been investigated to convert COBE to CHBE; this is the first approach to use engineering triad involving crp engineering, recombinant DNA engineering and transporter engineering together for improving cell performance during two-phase biocatalysis.
    Keywords biocatalysis ; Candida glabrata ; engineering ; Escherichia coli ; extrusion ; gene expression regulation ; genes ; genetic engineering ; models ; mutants ; nerolidol ; proteins ; quantitative polymerase chain reaction ; recombinant DNA ; reverse transcriptase polymerase chain reaction ; ribosomal DNA ; solvents
    Language English
    Dates of publication 2017-02
    Size p. 818-825.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2016.12.001
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Cationic guar gum orchestrated environmental synthesis for silver nano-bio-composite films

    Abdullah, Md Farooque / Arup Mukherjee / Sreyasree Basu / Sumanta Kumar Ghosh

    Carbohydrate polymers. 2015 Dec. 10, v. 134

    2015  

    Abstract: This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA). Assembly of molecules in lower size range (∼10nm) was attained in a biopolymer entrapped bottom–up ... ...

    Abstract This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA). Assembly of molecules in lower size range (∼10nm) was attained in a biopolymer entrapped bottom–up synthesis. Guar gum is a filming biopolymer. Nanoparticles encaged in cationic guar (GGAgnC) were preserved as films for months without any significant effect on particle size, distribution or plasmonic intensity. The new nano-bio-composite and films were characterized fully in FTIR, XRD, SEM and TEM studies. Silver nanoparticles induced surface water repellency remarkably and lowered moisture permeability. GGAgnC film water contact angle was recorded as 115° while, that in case of GGAA was 59°. GGAgnC expressed intense antimicrobial activity when tested against a range of microorganisms. Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
    Keywords anti-infective properties ; biopolymers ; contact angle ; fabrics ; Fourier transform infrared spectroscopy ; guar gum ; medical equipment ; microorganisms ; nanoparticles ; nanosilver ; packaging ; particle size ; permeability ; scanning electron microscopy ; silver ; surface water ; transmission electron microscopy ; X-ray diffraction
    Language English
    Dates of publication 2015-1210
    Size p. 30-37.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1501516-6
    ISSN 1879-1344 ; 0144-8617
    ISSN (online) 1879-1344
    ISSN 0144-8617
    DOI 10.1016/j.carbpol.2015.06.029
    Database NAL-Catalogue (AGRICOLA)

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