Article: Miniaturized, high numerical aperture confocal fluorescence detection enhanced with pyroelectric droplet accumulation for sub-attomole analyte diagnosis.
2023 Volume 14, Issue 12, Page(s) 6138–6150
Abstract: To meet the growing demand for early fatal disease screening among large populations, current fluorescence detection instruments aiming at point-of-care diagnosis have the tendency to be low cost and high sensitivity, with a high potential for the ... ...
| Abstract | To meet the growing demand for early fatal disease screening among large populations, current fluorescence detection instruments aiming at point-of-care diagnosis have the tendency to be low cost and high sensitivity, with a high potential for the analysis of low-volume, multiplex analytes with easy operation. In this work, we present the development of a miniaturized, high numerical aperture confocal fluorescence scanner for sub-micro-liter fluid diagnosis. It is enhanced with high-rate analyte accumulation using a pyroelectro-hydrodynamic dispensing system for generating tiny, stable sample droplets. The simplified confocal fluorescence scanner (numerical aperture 0.79, working distance 7.3 mm) uses merely off-the-shelf mass-production optical components. Experimental results show that it can achieve a high-sensitive, cost-efficient detection for sub-micro-liter, low-abundant (0.04 µL, 0.67 attomoles) fluid diagnosis, promising for point-of-care diagnosis. |
|---|---|
| Language | English |
| Publishing date | 2023-11-03 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2572216-5 |
| ISSN | 2156-7085 |
| ISSN | 2156-7085 |
| DOI | 10.1364/BOE.504757 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.