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Article ; Online: Transcriptomics and metabolomics reveal the adaptive mechanisms of Gracilariopsis lemaneiformis in response to blue light

Zhang, Bo / Sun, Deguang / Zhang, Xiaoqian / Sun, Xue / Xu, Nianjun

Algal Research. 2022 July, v. 66 p.102760-

2022

Abstract: The marine macroalgae Gracilariopsis lemaneiformis was cultured as the raw material of agar and abalone feed in China with the yield of 368,967 tons dry mass. As light quality is the most important environmental factor for the photosynthetic organism, ... ...

Abstract	The marine macroalgae Gracilariopsis lemaneiformis was cultured as the raw material of agar and abalone feed in China with the yield of 368,967 tons dry mass. As light quality is the most important environmental factor for the photosynthetic organism, and blue light is a main spectrum for photosynthesis and metabolite accumulation in plants, we test blue light for their effects on the G. lemaneiformis. Results showed that the relative growth rate of G. lemaneiformis decreased while the principal economical metabolites, including agar, polysaccharides were accumulated significantly under blue light. To further understand the response mechanism of G. lemaneiformis to blue light, the omics analysis was used, which revealed that blue light redirected metabolic pathways toward the synthesis of diverse primary and secondary metabolic products, divided into four categories, including photosynthesis, antioxidant system, energy metabolism and carbohydrate synthesis. Among them, the genes encoding photosynthetic pigments and phycobiliproteins increased significantly, but the carbon fixation pathway, the ASA-GSH cycle, and TCA cycle were down-regulated, and more carbon flowed into the synthesis pathway of agar and polysaccharides, which were consistent with the changes of physiological parameters. These findings helped us to understand the mechanism of G. lemaneiformis responding to blue light and provide a new perspective for the culture of high-agar content G. lemaneiformis.
Keywords	Gracilariopsis lemaneiformis ; abalone ; agar ; antioxidants ; blue light ; carbon ; carbon dioxide fixation ; environmental factors ; light quality ; macroalgae ; metabolites ; metabolomics ; photosynthesis ; phycobiliprotein ; raw materials ; research ; transcriptomics ; tricarboxylic acid cycle ; China ; Transcriptome ; Metabolome
Language	English
Dates of publication	2022-07
Publishing place	Elsevier B.V.
Document type	Article ; Online
ISSN	2211-9264
DOI	10.1016/j.algal.2022.102760
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Emodin Protects Against Acute Pancreatitis-Associated Lung Injury by Inhibiting NLPR3 Inflammasome Activation via Nrf2/HO-1 Signaling.

Gao, Zhenming / Sui, Jidong / Fan, Rong / Qu, Weikun / Dong, Xuepeng / Sun, Deguang

Drug design, development and therapy

2020 Volume 14, Page(s) 1971–1982

Abstract: Aim: Lung injury is a common complication of acute pancreatitis (AP), which leads to the development of acute respiratory distress syndrome and causes high mortality. In the present study, we investigated the therapeutic effect of emodin on AP-induced ... ...

Abstract	Aim: Lung injury is a common complication of acute pancreatitis (AP), which leads to the development of acute respiratory distress syndrome and causes high mortality. In the present study, we investigated the therapeutic effect of emodin on AP-induced lung injury and explored the molecular mechanisms involved. Materials and methods: Thirty male Sprague-Dawley rats were randomly divided into AP (n=24) and normal (n=6) groups. Rats in the AP group received a retrograde injection of 5% sodium taurocholate into the biliary-pancreatic duct and then randomly assigned to untreated, emodin, combined emodin and ML385, and dexamethasone (DEX) groups. Pancreatic and pulmonary injury was assessed using H&E staining. In in vitro study, rat alveolar epithelial cell line L2 cells were exposed to lipopolysaccharide and treated with emodin. Nrf2 siRNA pool was applied for the knockdown of Nrf2. The contents of the pro-inflammatory cytokines in the bronchoalveolar lavage fluid and lung were determined using enzyme-linked immunosorbent assay. The expressions of related mRNAs and proteins in the lung or L2 cells were detected using real-time polymerase chain reaction, Western blot, immunohistochemistry and immunofluorescence. Key findings: Emodin administration alleviated pancreatic and pulmonary injury of rats with AP. Emodin administration suppressed the production of proinflammatory cytokines, downregulated NLRP3, ASC and caspase-1 expressions and inhibited NF-κB nuclear accumulation in the lung. In addition, Emodin increased Nrf2 nuclear translocation and upregulated HO-1 expression. Moreover, the anti-inflammatory effect of emodin was blocked by Nrf2 inhibitor ML385. Conclusion: Emodin effectively protects rats against AP-associated lung injury by inhibiting NLRP3 inflammasome activation via Nrf2/HO-1 signaling.
MeSH term(s)	Acute Disease ; Acute Lung Injury/drug therapy ; Acute Lung Injury/metabolism ; Acute Lung Injury/pathology ; Administration, Oral ; Animals ; Cell Proliferation/drug effects ; Cells, Cultured ; Emodin/administration & dosage ; Emodin/pharmacology ; Heme Oxygenase-1/genetics ; Heme Oxygenase-1/metabolism ; Inflammasomes/drug effects ; Inflammasomes/metabolism ; Male ; NF-E2-Related Factor 2/metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein/antagonists & inhibitors ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Pancreatitis/drug therapy ; Pancreatitis/metabolism ; Pancreatitis/pathology ; Rats ; Rats, Sprague-Dawley
Chemical Substances	Inflammasomes ; NF-E2-Related Factor 2 ; NLR Family, Pyrin Domain-Containing 3 Protein ; Nlrp3 protein, rat ; Heme Oxygenase-1 (EC 1.14.14.18) ; Emodin (KA46RNI6HN)
Language	English
Publishing date	2020-05-21
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2451346-5
ISSN	1177-8881 ; 1177-8881
ISSN (online)	1177-8881
ISSN	1177-8881
DOI	10.2147/DDDT.S247103
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Case Report: An Undefined Liver Lesion in a Young Man With Severe Aplastic Anemia: A Teachable Moment.

Liu, Jin / Sui, Jidong / Sun, Deguang / Guo, Kun / Gao, Zhenming / Bian, Jie / Yan, Jinsong / Wang, Liming

Frontiers in surgery

2021 Volume 8, Page(s) 665367

Abstract: In this work, we reported a young man complaining of asthenia and intermittent fever for 10 days, and an ultrasound showed an undefined lesion on his liver. Facing the patient's situation with severe agranulocytosis, anemia, and thrombocytopenia, we ... ...

Abstract	In this work, we reported a young man complaining of asthenia and intermittent fever for 10 days, and an ultrasound showed an undefined lesion on his liver. Facing the patient's situation with severe agranulocytosis, anemia, and thrombocytopenia, we passed through a tough diagnostic process for choosing an appropriate treatment for him with an ambiguous result of pathological biopsy. The undefined liver lesion was successfully solved by withdrawing the androgen for observation, without lobectomy. The lesion gradually diminished over 2 years of follow-up.
Language	English
Publishing date	2021-07-14
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2773823-1
ISSN	2296-875X
ISSN	2296-875X
DOI	10.3389/fsurg.2021.665367
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Article ; Online: miR-186 affects the proliferation, invasion and migration of human gastric cancer by inhibition of Twist1.

Cao, Chunhong / Sun, Deguang / Zhang, Liang / Song, Lei

Oncotarget

2016 Volume 7, Issue 48, Page(s) 79956–79963

Abstract: Recent evidence shows that miRNAs are dysregulated in a variety of cancers including gastric cancer (GC), and emerging as key oncogenes or tumor suppressors. In this study, qRT-PCR was used to analyze the expression of miR-186 in GC tissues and adjacent ... ...

Abstract	Recent evidence shows that miRNAs are dysregulated in a variety of cancers including gastric cancer (GC), and emerging as key oncogenes or tumor suppressors. In this study, qRT-PCR was used to analyze the expression of miR-186 in GC tissues and adjacent non-cancerous tissues, and then more in-vitro experiments were used to investigate the role of miR-186 in GC cells. Here, we identified miR-186 was generally down-regulated in GC tissues; however, Twist1 was generally up-regulated in GC tissues. Moreover, miR-186 and Twist1 were associated with larger tumor size and advanced clinical stage of GC. In-vitro experiments demonstrated that ectopic overexpression of miR-186 inhibited GC cell proliferation, invasion and migration; however, inhibited expression of miR-186 enhanced cell proliferation, invasion and migration. Furthermore, the luciferase reporter assay demonstrated Twist1 as a direct target of miR-186. Finally, over-expression of Twist1 abrogated inhibitory impact of miR-186 on cell proliferation, invasion and migration. In conclusion, miR-186 affects the proliferation, invasion and migration of human gastric cancer by inhibition of Twist1, and could be a tumor suppressor in GC development. Thus, miR-186 may be served as a candidate prognostic biomarker and target for new therapies in human gastric cancer.
Language	English
Publishing date	2016-11-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	2560162-3
ISSN	1949-2553 ; 1949-2553
ISSN (online)	1949-2553
ISSN	1949-2553
DOI	10.18632/oncotarget.13182
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: MiR-3196, a p53-responsive microRNA, functions as a tumor suppressor in hepatocellular carcinoma by targeting FOXP4.

Qi, Wenjing / Gao, Chengshun / Zhang, Li / Gao, Zhenming / Sui, Jidong / Han, Chuanchun / Sun, Deguang

American journal of cancer research

2019 Volume 9, Issue 12, Page(s) 2665–2678

Abstract: Increasing evidences demonstrate that miRNAs play an important role in development and progression of hepatocellular carcinoma (HCC). Recent studies indicate that miR-3196 regulates tumorigenesis in breast and lung cancer. However, its role and ... ...

Abstract	Increasing evidences demonstrate that miRNAs play an important role in development and progression of hepatocellular carcinoma (HCC). Recent studies indicate that miR-3196 regulates tumorigenesis in breast and lung cancer. However, its role and regulatory mechanism remains unknown in hepatocellular carcinoma. Here, we found that miR-3196 was downregulated in HCC tissues and decreased miR-3196 was correlated with tumor size (
Language	English
Publishing date	2019-12-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2589522-9
ISSN	2156-6976
ISSN	2156-6976
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Deep sequencing and comprehensive expression analysis identifies several molecules potentially related to human poorly differentiated hepatocellular carcinoma.

Shao, Ping / Sun, Deguang / Wang, Liming / Fan, Rong / Gao, Zhenming

FEBS open bio

2017 Volume 7, Issue 11, Page(s) 1696–1706

Abstract: Hepatocellular carcinoma (HCC) that is graded histologically as poorly differentiated has a high recurrence, metastasis and poor prognosis. We sought to determine the regulatory mechanisms of HCC tumorigenesis and to identify molecules closely related to ...

Abstract	Hepatocellular carcinoma (HCC) that is graded histologically as poorly differentiated has a high recurrence, metastasis and poor prognosis. We sought to determine the regulatory mechanisms of HCC tumorigenesis and to identify molecules closely related to poorly differentiated HCC. High-throughput sequencing was used to construct microRNA (miRNA) and mRNA expression profiles for poorly differentiated HCC tissues and adjacent tissues. Network analysis was carried out to study miRNA-target interactions. Integrating the miRNA and mRNA data of HCC with four tumor grades from The Cancer Genome Atlas (TCGA) portal enabled the identification of potential closely related molecules for early diagnosis of poorly differentiated HCC. Electronic validation of RNA-seq data and survival analysis was also performed. In total, 1051 differentially expressed genes and 165 differentially expressed miRNAs were identified between HCC tumor and paired non-tumorous tissue. Based on 3718 miRNA-target interactions, we established an miRNA-target interaction network; the target genes were mainly involved in bile acid biosynthesis and bile secretion. Integrating expression data of HCC from TCGA indicated that two proteins, TM4SF1 and ANXA2, are convincing indicators for initial diagnosis of poorly differentiated HCC. According to the survival analysis, three proteins, ANXA2, C8orf33 and IGF2BP3, were identified as being associated with the survival time of HCC patients. Moreover, we suggest that hsa-miR-1180 may be an effective biomarker for poorly differentiated HCC. Three molecules, TM4SF1, ANXA2 and C8orf33, are potential biomarkers for distinguishing poorly differentiated from well-differentiated HCC.
Language	English
Publishing date	2017
Publishing country	England
Document type	Journal Article
ISSN	2211-5463
ISSN	2211-5463
DOI	10.1002/2211-5463.12310
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: [Corrigendum] Metformin reverses multidrug resistance in human hepatocellular carcinoma Bel‑7402/5‑fluorouracil cells.

Ling, Sunbin / Tian, Yu / Zhang, Haiquan / Jia, Kaiqi / Feng, Tingting / Sun, Deguang / Gao, Zhenming / Xu, Fei / Hou, Zhaoyuan / Li, Yan / Wang, Liming

Molecular medicine reports

2022 Volume 25, Issue 5

Abstract: Subsequently to the publication of the above paper, the authors have reviewed its content and the primary data, and have realized that the western blots selected to show the β‑actin experiments featured in Fig. 4A and Fig. 3C were the same blot, albeit ... ...

Abstract	Subsequently to the publication of the above paper, the authors have reviewed its content and the primary data, and have realized that the western blots selected to show the β‑actin experiments featured in Fig. 4A and Fig. 3C were the same blot, albeit with a different exposure time. The control blots correctly presented for Fig. 3C were inadvertently copied into Fig. 4A owing to an error made during the figure compilation process. The revised version of Fig. 4, containing the correct β‑actin blots for Fig. 4A, is shown below. Note that this error did not significantly affect the results or the conclusions reported in this paper, and all the authors agree to this Corrigendum. The authors thank the Editor of
Language	English
Publishing date	2022-03-22
Publishing country	Greece
Document type	Published Erratum
ZDB-ID	2469505-1
ISSN	1791-3004 ; 1791-2997
ISSN (online)	1791-3004
ISSN	1791-2997
DOI	10.3892/mmr.2022.12691
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Molecular cloning of the cpeT gene encoding a bilin lyase responsible for attachment of phycoerythrobilin to Cys-158 on the β-subunit of phycoerythrin in Gracilariopsis lemaneiformis

Cao, Xuexue / Guo, Yalin / Jin, Yuming / Lin, Jiaojiao / Liu, Zhu / Sun, Deguang / Wang, Zhendong / Zang, Xiaonan / Zhang, Feng

Journal of applied phycology. 2019 Oct., v. 31, no. 5

2019

Abstract: Phycobilin lyase plays an important role in the binding of phycobilin chromophores to phycobiliprotein. The bilin lyase cpeT gene was cloned from Gracilariopsis lemaneiformis to study the synthesis of fluorescent phycobiliprotein. Two recombinant ... ...

Abstract	Phycobilin lyase plays an important role in the binding of phycobilin chromophores to phycobiliprotein. The bilin lyase cpeT gene was cloned from Gracilariopsis lemaneiformis to study the synthesis of fluorescent phycobiliprotein. Two recombinant plasmids, one containing the genes (ho1 and pebA, pebB) that produce phycoerythrobilin and the other containing the two subunit genes (rpeB, rpeA) of phycoerythrin (R-PE), as well as the lyase gene cpeT, were constructed and transformed into Escherichia coli. The recombinant strain with lyase CpeT and the β-subunit of phycoerythrin (R-PeB) enhanced the optical activity of R-PE, indicating that lyase CpeT catalyzes the synthesis of the R-PeB with optical activity. Another two recombinant plasmids, one containing the genes (ho(314), pcyA(314)) that produce phycocyanobilin and the other containing the two subunit genes (rpcB, rpcA) of phycocyanin (R-PC), as well as the lyase gene cpeT, were constructed and transformed into Escherichia coli. However, there was no enhancement in fluorescence in this recombinant strain, revealing that lyase CpeT cannot catalyze the synthesis of optically active R-PC. Thereafter, Cys-82 and Cys-158 of R-PeB were individually mutated to identify the possible binding sites of apoproteins with phycobilins catalyzed by CpeT. We found that there was a significant decrease in the fluorescence of the mutated Cys-158 recombinant strain, suggesting that Cys-158 is the active site for the binding of the β-subunit with CpeT-catalyzed phycoerythrobilin in G. lemaneiformis. The results of this study lay the foundation for understanding the synthesis of fluorescent phycobiliprotein.
Keywords	active sites ; apoproteins ; binding sites ; catalytic activity ; Escherichia coli ; fluorescence ; genes ; Gracilariopsis lemaneiformis ; molecular cloning ; optical properties ; phycobilin ; phycocyanin ; phycoerythrin ; plasmids
Language	English
Dates of publication	2019-10
Size	p. 3331-3340.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	1002324-0
ISSN	1573-5176 ; 0921-8971
ISSN (online)	1573-5176
ISSN	0921-8971
DOI	10.1007/s10811-019-01778-w
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Article: One type of duplex appendix: horseshoe appendix.

Liu, Jin / Dong, Chengyong / Wang, Haibo / Sun, Deguang / Liang, Rui / Gao, Zhenming / Wang, Liming

Therapeutics and clinical risk management

2018 Volume 14, Page(s) 1987–1992

Abstract: A horseshoe appendix is a subtype of duplex appendix, in which the appendix is shaped like a circle that may lead to an internal hernia and result in intestinal necrosis. This subtype is extremely rare, with only 13 cases reported worldwide to date, and ... ...

Abstract	A horseshoe appendix is a subtype of duplex appendix, in which the appendix is shaped like a circle that may lead to an internal hernia and result in intestinal necrosis. This subtype is extremely rare, with only 13 cases reported worldwide to date, and easily triggers a series of medico-legal consequences due to the neglect of another infection base of the appendix. We describe a 22-year-old man who presented with a 3-day history of fever and was diagnosed with pneumonia. After receiving antibiotics for 3 days in the Department of Pneumology, he was found to have a periappendiceal abscess. He underwent appendectomy after 3 days of conservative treatment failure in the Department of General Surgery. During the operation, we found that he had a horseshoe appendix with the two bases forming a circle, each communicating with the cecum. We provide a review of 13 cases presented in the literature, with a discussion of the clinical features, diagnosis, and surgical approach of the horseshoe appendix to make the general surgeon get a clear concept of this type of appendicitis.
Language	English
Publishing date	2018-10-12
Publishing country	New Zealand
Document type	Case Reports
ZDB-ID	2186560-7
ISSN	1178-203X ; 1176-6336
ISSN (online)	1178-203X
ISSN	1176-6336
DOI	10.2147/TCRM.S179929
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Article: Cloning and expression of Allophycocyanin gene from Gracilariopsis lemaneiformis and studying the binding sites of phycocyanobilin on its α and β subunits

Guo, Yalin / Zang, Xiaonan / Cao, Xuexue / Zhang, Feng / Sun, Deguang / Shang, Menghui / Li, Rui / Yangzong, Zhaxi / Wei, Xuehong / Zhang, Xuecheng

Journal of applied phycology. 2020 Aug., v. 32, no. 4

2020

Abstract: Allophycocyanin (APC) is a pigment-protein with optical activity in the core of phycobilisomes of cyanobacteria and red algae. Its wide application prospects make it necessary to carry out the recombinant expression of allophycocyanin with optical ... ...

Abstract	Allophycocyanin (APC) is a pigment-protein with optical activity in the core of phycobilisomes of cyanobacteria and red algae. Its wide application prospects make it necessary to carry out the recombinant expression of allophycocyanin with optical activity. In this study, apcA and apcB genes encoding two subunits of allophycocyanin were cloned from Gracilariopsis lemaneiformis. Then apcA and apcB genes expressed in Escherichia coli to obtain allophycocyanin. In order to investigate the active sites of allophycocyanin to bind with phycocyanobilin, the Cys-81 and Cys-138 of α subunit and Cys-81 and Cys-157 of β subunit were mutated. SDS-PAGE and Western blotting results verified the expression of allophycocyanin. The fluorescence emission spectra showed the characteristic fluorescence peak of allophycocyanin, which indicated that the recombinant allophycocyanin had optical activity. The recombinant strains with chromophore lyases—CpcU and CpcS—had the higher fluorescence emission peak, indicating that chromophore lyases would catalyze the combination of phycocyanobilin and apo-allophycocyanin more effectively. The binding sites were Cys-81 and Cys138 of allophycocyanin α subunit, and Cys-81 and Cys-157 of allophycocyanin β subunit. The catalytic effect of CpeT was not obvious. This research provides an experimental foundation for understanding the synthesis mechanism of optically active allophycocyanin in G. lemaneiformis.
Keywords	Escherichia coli ; Gracilariopsis lemaneiformis ; algology ; fluorescence ; genes ; lyases ; optical properties ; phycobilisome ; polyacrylamide gel electrophoresis
Language	English
Dates of publication	2020-08
Size	p. 2657-2671.
Publishing place	Springer Netherlands
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	1002324-0
ISSN	1573-5176 ; 0921-8971
ISSN (online)	1573-5176
ISSN	0921-8971
DOI	10.1007/s10811-020-02102-7
Database	NAL-Catalogue (AGRICOLA)

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