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Article ; Online: Protective efficacy of

Sun, Hong-Chao / Deng, Pu-Ming / Fu, Yuan / Deng, Jin-Hua / Xie, Rong-Hui / Huang, Jing / Qi, Meng / Shi, Tuan-Yuan

Frontiers in cellular and infection microbiology

2023 Volume 13, Page(s) 1209755

Abstract: Background: Toxoplasma gondii is an apicomplexan parasite that affects the health of humans and livestock, and an effective vaccine is urgently required. Nanoparticles can modulate and improve cellular and humoral immune responses.: Methods: In the ... ...

Abstract	Background: Toxoplasma gondii is an apicomplexan parasite that affects the health of humans and livestock, and an effective vaccine is urgently required. Nanoparticles can modulate and improve cellular and humoral immune responses. Methods: In the current study, poly (D, L-lactic-co-glycolic acid) (PLGA) nanoparticles were used as a delivery system for the T. gondii dense granule antigens GRA12 and GRA7. BALB/c mice were injected with the vaccines and protective efficacy was evaluated. Results: Mice immunized with PLGA+GRA12 exhibited significantly higher IgG, and a noticeable predominance of IgG2a over IgG1 was also observed. There was a 1.5-fold higher level of lymphocyte proliferation in PLGA+GRA12-injected mice compared to Alum+GRA12-immunized mice. Higher levels of IFN-g and IL-10 and a lower level of IL-4 were detected, indicating that Th1 and Th2 immune responses were induced but the predominant response was Th1. There were no significant differences between Alum+GRA7-immunized and PLGA+GRA7-immunized groups. Immunization with these four vaccines resulted in significantly reduced parasite loads, but they were lowest in PLGA+GRA12-immunized mice. The survival times of mice immunized with PLGA+GRA12 were also significantly longer than those of mice in the other vaccinated groups. Conclusion: The current study indicated that T. gondii GRA12 recombinant protein encapsulated in PLGA nanoparticles is a promising vaccine against acute toxoplasmosis, but PLGA is almost useless for enhancing the immune response induced by T. gondii GRA7 recombinant protein.
MeSH term(s)	Humans ; Animals ; Mice ; Toxoplasma ; Protozoan Proteins/genetics ; Antigens, Protozoan/genetics ; Toxoplasmosis ; Recombinant Proteins ; Lactic Acid ; Nanoparticles ; Mice, Inbred BALB C ; Protozoan Vaccines ; Antibodies, Protozoan
Chemical Substances	aluminum sulfate (34S289N54E) ; Protozoan Proteins ; Antigens, Protozoan ; Recombinant Proteins ; Lactic Acid (33X04XA5AT) ; Protozoan Vaccines ; Antibodies, Protozoan
Language	English
Publishing date	2023-07-12
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2023.1209755
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Article ; Online: Molecular characterization and protective efficacy of vacuolar protein sorting 29 from

Shi, Tuan-Yuan / Li, Tian-En / Hao, Yun / Sun, Hong-Chao / Fu, Yuan / Yan, Wen-Chao / Hao, Li-Li

Frontiers in cellular and infection microbiology

2023 Volume 13, Page(s) 1205782

Abstract: Introduction: Vacuolar protein sorting 29 (VPS29) is a core component of the retromer-retriever complex and is essential for recycling numerous cell-surface cargoes from endosomes. However, there are no reports yet on VPS29 of : Methods: Here, we ... ...

Abstract	Introduction: Vacuolar protein sorting 29 (VPS29) is a core component of the retromer-retriever complex and is essential for recycling numerous cell-surface cargoes from endosomes. However, there are no reports yet on VPS29 of Methods: Here, we cloned and prokaryotically expressed a partial sequence of Results: An EtVPS29 gene fragment with an ORF reading frame of 549 bp was cloned. The band size of the expressed recombinant protein, rEtVPS29, was approximately 39 kDa and was recognized by the chicken anti- Discussion: These results suggest that rEtVPS29 can induce a specific immune response and is a potential candidate for the development of novel vaccines against
MeSH term(s)	Animals ; Eimeria tenella/genetics ; Chickens ; Recombinant Proteins/metabolism ; Immunization ; Vaccination/veterinary ; Oocysts/metabolism ; Sporozoites ; Poultry Diseases/prevention & control ; Protozoan Vaccines/genetics
Chemical Substances	Recombinant Proteins ; Protozoan Vaccines
Language	English
Publishing date	2023-07-04
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2023.1205782
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Article ; Online: Dominant infection of Cryptosporidium baileyi in broiler chickens in Zhejiang Province, China.

Feng, Xinwei / Deng, Jinhua / Zhang, Zhenjie / Yu, Fuchang / Zhang, Jianing / Shi, Tuanyuan / Sun, Hongchao / Qi, Meng / Liu, Xuehan

Parasitology research

2023 Volume 122, Issue 9, Page(s) 1993–2000

Abstract: Cryptosporidium is a common enteric parasite in chickens. A total of 812 fecal specimens were collected from 11 broiler farms in Zhejiang Province, China, and analyzed by nested PCR amplification based on the small subunit ribosomal RNA (SSU rRNA) gene. ... ...

Abstract	Cryptosporidium is a common enteric parasite in chickens. A total of 812 fecal specimens were collected from 11 broiler farms in Zhejiang Province, China, and analyzed by nested PCR amplification based on the small subunit ribosomal RNA (SSU rRNA) gene. The overall infection rate of Cryptosporidium was 6.3% (51/812), and five of 11 farms were Cryptosporidium positive. Broilers aged > 90 days accounted for the highest infection rate of 16.1% (6/56), followed by those aged 30-60 days (10.6%, 38/358) and 60-90 days (4/378, 1.1%). Two Cryptosporidium species were identified by sequence analysis, with the predominant species being C. baileyi (96.1%, 49/51) and the minor infection being C. meleagridis (3.9%, 2/51). Based on the 60-kDa glycoprotein (gp60) gene, two C. meleagridis-positive isolates were identified as one known subtype, IIIbA24G1R1. This study indicated the common occurrence of C. baileyi in broiler chickens in this region and low zoonotic transmission potential of Cryptosporidium to humans.
MeSH term(s)	Humans ; Animals ; Cryptosporidium ; Chickens/parasitology ; Cryptosporidiosis/parasitology ; Poultry Diseases/parasitology ; China/epidemiology ; Feces/parasitology ; Genotype
Language	English
Publishing date	2023-06-22
Publishing country	Germany
Document type	Journal Article
ZDB-ID	284966-5
ISSN	1432-1955 ; 0932-0113 ; 0044-3255
ISSN (online)	1432-1955
ISSN	0932-0113 ; 0044-3255
DOI	10.1007/s00436-023-07898-0
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Article ; Online: Pathogenicity and drug resistance of the Eimeria tenella isolate from Yiwu, Zhejiang province, eastern China.

Sun, Hongchao / Su, Xinyao / Fu, Yuan / Hao, Lili / Zhou, Wei / Zhou, Zhijin / Huang, Jing / Wang, Yimin / Shi, Tuanyuan

Poultry science

2023 Volume 102, Issue 8, Page(s) 102845

Abstract: Chicken coccidiosis can cause severe enteritis with high mortality, which causes serious economic losses to the global breeding industry each year. The most virulent species is Eimeria tenella (E. tenella), but the infectivity of different E. tenella ... ...

Abstract	Chicken coccidiosis can cause severe enteritis with high mortality, which causes serious economic losses to the global breeding industry each year. The most virulent species is Eimeria tenella (E. tenella), but the infectivity of different E. tenella varies among geographic strains. At present, there are no reports related to the pathogenicity and drug resistance of E. tenella in Yiwu, Zhejiang province, China. A total of 600 fecal samples were collected from 10 farms in Zhejiang province, the overall oocyst prevalence was 54.2% (325/600). The prevalence was significantly higher (P < 0.01) in chickens under 40 d (97.5%) than that in chickens between 60 and 85-days-old (40.5%) and chickens over 90-days-old (24.5%). E. tenella stain was isolated from fecal samples of chickens in Yiwu and the pathogenicity of this isolate was determined, and then we recorded the survival rate, bloody stool score, lesion score, average weight gain. The results showed that all of the chickens infected with 5 × 10
MeSH term(s)	Animals ; Eimeria tenella ; Clopidol ; Nicarbazin ; Virulence ; Chickens ; Coccidiosis/epidemiology ; Coccidiosis/veterinary ; Weight Gain ; Drug Resistance ; Poultry Diseases/epidemiology
Chemical Substances	sulfachlorpyrazine (96R84UZ2XA) ; Clopidol (8J763HFF5N) ; Nicarbazin (11P9NUA12U)
Language	English
Publishing date	2023-06-05
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2023.102845
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Article ; Online: Mimicking Bidirectional Inhibitory Synapse Using a Porous-Confined Ionic Memristor with Electrolyte/Tris(4-aminophenyl)amine Neurotransmitter.

Chen, Kang / Pan, Keyuan / He, Shang / Liu, Rui / Zhou, Zhe / Zhu, Duoyi / Liu, Zhengdong / He, Zixi / Sun, Hongchao / Wang, Min / Wang, Kaili / Tang, Minghua / Liu, Juqing

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

2024 , Page(s) e2400966

Abstract: Ionic memristors can emulate brain-like functions of biological synapses for neuromorphic technologies. Apart from the widely studied excitatory-excitatory and excitatory-inhibitory synapses, reports on memristors with the inhibitory-inhibitory synaptic ... ...

Abstract	Ionic memristors can emulate brain-like functions of biological synapses for neuromorphic technologies. Apart from the widely studied excitatory-excitatory and excitatory-inhibitory synapses, reports on memristors with the inhibitory-inhibitory synaptic behaviors remain a challenge. Here, the first biaxially inhibited artificial synapse is demonstrated, consisting of a solid electrolyte and conjugated microporous polymers bilayer as neurotransmitter, with the former serving as an ion reservoir and the latter acting as a confined transport. Due to the migration, trapping, and de-trapping of ions within the nanoslits, the device poses inhibitory synaptic plasticity under both positive and negative stimuli. Remarkably, the artificial synapse is able to maintain a low level of stable nonvolatile memory over a long period of time (≈60 min) after multiple stimuli, with feature-inferencing/-training capabilities of neural node in neuromorphic computing. This work paves a reliable strategy for constructing nanochannel ionic memristive materials toward fully inhibitory synaptic devices.
Language	English
Publishing date	2024-03-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2808093-2
ISSN	2198-3844 ; 2198-3844
ISSN (online)	2198-3844
ISSN	2198-3844
DOI	10.1002/advs.202400966
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Article ; Online: Enhancing Immune Responses to a DNA Vaccine Encoding

Sun, Hong-Chao / Huang, Jing / Fu, Yuan / Hao, Li-Li / Liu, Xin / Shi, Tuan-Yuan

Frontiers in cellular and infection microbiology

2021 Volume 11, Page(s) 787635

Abstract: Toxoplasma ... ...

Abstract	Toxoplasma gondii
MeSH term(s)	Animals ; Antibodies, Protozoan ; Antigens, Protozoan/genetics ; Calcium Phosphates ; Immunity, Humoral ; Mice ; Mice, Inbred BALB C ; Nanoparticles ; Protozoan Proteins/genetics ; Protozoan Vaccines ; Toxoplasma/genetics ; Vaccines, DNA
Chemical Substances	Antibodies, Protozoan ; Antigens, Protozoan ; Calcium Phosphates ; Protozoan Proteins ; Protozoan Vaccines ; Vaccines, DNA
Language	English
Publishing date	2021-12-16
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2021.787635
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Article: The relative importance of driving factors of wildfire occurrence across climatic gradients in the Inner Mongolia, China

Sun, Hongchao / Wang, Wen J. / Liu, Zhihua / Zou, Xianghua / Zhang, Zhengxiang / Ying, Hong / Dong, Yulin / Yang, Ran

Ecological indicators. 2021 Nov., v. 131

2021

Abstract: In recent decades, wildfire regimes have been changing in many parts of the world with significant differences among climatic regions. Thus, understanding the relationship between fires and driving factors in different climatic regions is important. We ... ...

Abstract	In recent decades, wildfire regimes have been changing in many parts of the world with significant differences among climatic regions. Thus, understanding the relationship between fires and driving factors in different climatic regions is important. We investigated the relative importance of wildfire drivers across climatic gradients using a Bayesian network model during 2001–2015 in the Inner Mongolia Autonomous Region (IMAR). Our results showed that regional-scale fire relative probability (FRP) on average increased in the IMAR, which was mainly attributed to increases in annual precipitation. However, changes in FRP and driving factors of wildfire occurrence varied among climatic regions. The FRP decreased at rates of −0.02/decade and −0.001/decade in the humid and semi-humid region, respectively, while increasing at an average rate of 0.05/ decade in the arid region. Temperature was recognized as the most influential driver of wildfires in the humid region, in which the relative importance of all driving factors remained relatively stable over time. In the semi-humid region, wildfires responded primarily to human activities, of which distance to railways was of greatest importance. The relative importance of distance to railways significantly increased over time, while the effects of temperature, humidity and elevation decreased considerably. Fractional vegetation cover (FVC) was the dominant factor controlling the wildfire occurrence in the arid region, in which the relative importance of precipitation significantly increased and the effect of settlements significantly decreased. Our study suggests that because of different driving factors of wildfires across climatic gradients, different fire prevention and management strategies are required. The optimal resource allocation for fire prevention should be adjusted according to the relative importance of wildfire drivers and spatiotemporal patterns of the FRP.
Keywords	Bayesian theory ; arid zones ; atmospheric precipitation ; fire prevention ; fractional vegetation cover ; humans ; humid zones ; humidity ; models ; probability ; resource allocation ; temperature ; wildfires ; China
Language	English
Dates of publication	2021-11
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2036774-0
ISSN	1872-7034 ; 1470-160X
ISSN (online)	1872-7034
ISSN	1470-160X
DOI	10.1016/j.ecolind.2021.108249
Database	NAL-Catalogue (AGRICOLA)

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Article: Molecular Detection and Genotyping of Toxoplasma gondii from Pigs for Human Consumption in Zhejiang and Jiangsu Provinces, Eastern China

Sun, Hong-Chao / Fu, Yuan / Yuan, Xiu-Fang / Li, Jun-Xing / Xu, Li-hua / Zhang, Jia-Ning / Yu, Bin / Huang, Jing / Qi, Meng / Shi, Tuan-Yuan

Foodborne pathogens & disease. 2022 Sept. 30,

2022

Abstract: Toxoplasma gondii infections are common in humans and animals worldwide. Ingestion of raw or undercooked meat containing tissue cysts of T. gondii is one major source of transmission of this parasite. It is important to guarantee the meat quality of ... ...

Abstract	Toxoplasma gondii infections are common in humans and animals worldwide. Ingestion of raw or undercooked meat containing tissue cysts of T. gondii is one major source of transmission of this parasite. It is important to guarantee the meat quality of China since our pork industry produces about half of the world's pork. In this study, a total of 746 pig samples were collected from Zhejiang and Jiangsu provinces in eastern China, and examined for T. gondii infection by PCR amplification targeting B1 gene. In this study, we found that 57 of 746 (7.6%) pigs were positive for B1 gene, with 8.5% (48/562) in Zhejiang province and 4.9% (9/184) in Jiangsu province, respectively. The positive DNA samples were further genotyped at 11 genetic markers, including SAG1, 5′-and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and an apicoplast locus Apico through PCR–restriction fragment length polymorphism (PCR-RFLP) technology. Two genotypes (ToxoDB 9 and ToxoDB 10) of T. gondii were identified by PCR-RFLP in Zhejiang province. However, both genotypes were not determined from Jiangsu province, which is speculated on the low DNA concentration and the small number of samples. These results indicate that T. gondii infection is endemic in pigs in eastern China and may raise public food safety concerns, suggesting more interventions for T. gondii-related risks are needed in the future.
Keywords	DNA ; Toxoplasma gondii ; food safety ; genes ; genotyping ; humans ; ingestion ; loci ; meat quality ; parasites ; pork ; pork industry ; swine ; China
Language	English
Dates of publication	2022-0930
Publishing place	Mary Ann Liebert, Inc.
Document type	Article
ZDB-ID	2148479-X
ISSN	1556-7125 ; 1535-3141
ISSN (online)	1556-7125
ISSN	1535-3141
DOI	10.1089/fpd.2022.0036
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Article ; Online: Influence of Air Volume and Temperature in the Air Inlet Tunnel on the Characteristics of Dust Movement.

Zhu, YeMing / Chen, Tao / Sun, ShuTang / Ji, Ying / Zhao, Dong / Zhuang, DaJie / Lian, YiRen / Rong, Yu / Yan, Jin / Sun, HongChao

ACS omega

2023 Volume 9, Issue 1, Page(s) 866–878

Abstract: In order to clarify the movement characteristics of dust particles in the intake tunnel and improve the underground intake tunnel environment, the main intake tunnel of Wulihou Coal Mine was taken as the engineering background, the COMSOL simulation ... ...

Abstract	In order to clarify the movement characteristics of dust particles in the intake tunnel and improve the underground intake tunnel environment, the main intake tunnel of Wulihou Coal Mine was taken as the engineering background, the COMSOL simulation software was adopted to establish a model, the influence of air volume and temperature on dust movement characteristics was studied, and the critical air volume and particle size of dust at room temperature were determined. The results show that with the ventilation air volume being the same, when the dust reaches the exit of the tunnel, its falling height is positively correlated with the particle size. When the dust particle size is the same, the height of the dust falling is negatively correlated with the ventilation air volume. As the particle size of dust increases, the impact of changes in air volume on its movement decreases and the trajectory of dust movement gradually becomes consistent. The height of dust falling is negatively correlated with the air flow temperature. Therefore, when other factors remain unchanged, dust pollution in the tunnel is relatively severe during the day and the dust concentration in the tunnel is higher in summer. The critical air volume for dust emission in the intake tunnel of Wulihou Coal Mine is 75.84 m
Language	English
Publishing date	2023-12-04
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.3c06816
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Article ; Online: PP2Acα-B'/PR61 Holoenzyme of Toxoplasma gondii Is Required for the Amylopectin Metabolism and Proliferation of Tachyzoites.

Zhao, Mingxiu / Yang, Yi / Shi, Yue / Chen, Xueqiu / Yang, Yimin / Pan, Lingtao / Du, Zhendong / Sun, Hongchao / Yao, Chaoqun / Ma, Guangxu / Du, Aifang

Microbiology spectrum

2023 Volume 11, Issue 3, Page(s) e0010423

Abstract: Here, we report that the inhibition of the PP2A subfamily by okadaic acid results in an accumulation of polysaccharides in the acute infection stage (tachyzoites) of Toxoplasma gondii, which is a protozoan of global zoonotic importance and a model for ... ...

Abstract	Here, we report that the inhibition of the PP2A subfamily by okadaic acid results in an accumulation of polysaccharides in the acute infection stage (tachyzoites) of Toxoplasma gondii, which is a protozoan of global zoonotic importance and a model for the apicomplexan parasites. The loss of the catalytic subunit α of PP2A (Δ
MeSH term(s)	Animals ; Toxoplasma/genetics ; Parasites ; Amylopectin ; Cell Proliferation ; Holoenzymes/metabolism ; Glucose/metabolism
Chemical Substances	Amylopectin (9037-22-3) ; Holoenzymes ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2023-05-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.00104-23
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