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Article ; Online: Colorimetric sensor array for the rapid distinction and detection of various antibiotic-resistant psychrophilic bacteria in raw milk based-on machine learning.

Qin, Yanan / Sun, Jingshuai / Huang, Wanting / Yue, Haitao / Meng, Fanxing / Zhang, Minwei

Food chemistry: X

2024 Volume 22, Page(s) 101281

Abstract: In this study, a rapid, inexpensive, and accurate colorimetric sensor for detecting psychrophilic bacteria was designed, comprising gold (Au) nanoparticles (NPs) modified by d-amino acid (D-AA) as color-metric probes. Based on the aggregation of Au NPs ... ...

Abstract	In this study, a rapid, inexpensive, and accurate colorimetric sensor for detecting psychrophilic bacteria was designed, comprising gold (Au) nanoparticles (NPs) modified by d-amino acid (D-AA) as color-metric probes. Based on the aggregation of Au NPs induced by psychrophilic bacteria, a noticeable color shift occurred within 6 h. Depending on the various metabolic behaviors of bacteria to different D-AA, four primary psychrophilic bacteria in raw milk were successfully distinguished by learning the response patterns. Furthermore, the quantification of single bacteria and the practical application in milk samples could be realized. Notably, a rapid colorimetric method was constructed by combining Au/D-AA with antibiotics for the minimum inhibitory concentration of psychrophilic bacteria, which relied on differences in bacteria metabolic activity in response to diverse antibiotic treatments. Therefore, the method enables the rapid detection and susceptibility evaluation of psychrophilic bacteria, promoting clinical practicability and antibiotic management.
Language	English
Publishing date	2024-03-15
Publishing country	Netherlands
Document type	Journal Article
ISSN	2590-1575
ISSN (online)	2590-1575
DOI	10.1016/j.fochx.2024.101281
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Article: A Recombinant Oncolytic Pseudorabies Virus Expressing

Han, Xiaohui / Sun, Jingshuai / Lv, Xiaocheng / Tang, Xiaoyu / Zheng, Yubin / Ma, Jinyun / Sun, Yuan

Microorganisms

2023 Volume 11, Issue 7

Abstract: Pseudorabies virus (PRV) is considered to be a promising oncolytic virus that has potential as a cancer gene therapy drug. In this study, PRV-DCD-1-70 was used as a vector to carry exogenous ... ...

Abstract	Pseudorabies virus (PRV) is considered to be a promising oncolytic virus that has potential as a cancer gene therapy drug. In this study, PRV-DCD-1-70 was used as a vector to carry exogenous genes
Language	English
Publishing date	2023-07-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms11071850
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Article ; Online: Motility-related microRNAs identified in pig seminal plasma exosomes by high-throughput small RNA sequencing.

Zhao, Yunxiang / Qin, Jiali / Sun, Jingshuai / He, Jian / Sun, Yanmei / Yuan, Renqiang / Li, Zhili

Theriogenology

2023 Volume 215, Page(s) 351–360

Abstract: Boar fertility is a key determinant of the production efficiency of the whole pig breeding industry and boar sperm motility is the seminal parameter with the greatest impact on the fecundity of a sow. Exosomes are small, extracellular vesicles found in ... ...

Abstract	Boar fertility is a key determinant of the production efficiency of the whole pig breeding industry and boar sperm motility is the seminal parameter with the greatest impact on the fecundity of a sow. Exosomes are small, extracellular vesicles found in many body fluids. Seminal plasma exosomes, which are secreted by the epididymis, prostate, seminal vesicles, and testes, contain a large number of miRNAs, the types and levels of which can reflect the physiological state of source cells. It has been shown that the expression profile of seminal plasma exosomal miRNA differs between low-motility semen and normal semen. The aim of this study was to investigate the relationship between semen motility and exosomal miRNA profiles to obtain information that would allow to predict boar fertility, as well as contribute to the understanding of the mechanisms by which exosomal miRNAs regulate semen motility. Three high-motility (semen motility >90 %) and three low-motility (semen motility <80 %) semen samples were collected from Landrace and Yorkshire boars, respectively, and seminal plasma exosomes were extracted by ultracentrifugation. Exosome characterization was performed using transmission electron microscopy, NTA, and Western blot. The expression profiles of exosomal miRNAs associated with semen motility in the two boar breeds were subsequently determined by small RNA sequencing. The results showed that 297 known miRNAs and 295 novel RNAs were co-expressed in the four groups. Notably, six miRNAs (ssc-miR-122-5p, ssc-miR-486, ssc-miR-451, ssc-miR-345-3p, ssc-miR-362, and ssc-miR-500-5p) were found to be differentially expressed in both boar breeds. Enrichment analysis of the target genes of the differentially expressed miRNAs showed that they were mainly involved in biological processes such as regulation of transcription from RNA polymerase II promoter, regulation of gene expression, and intracellular signal transduction and signaling pathways such as the PI3K-Akt, MAPK, and Ras signaling pathways. The six differentially expressed miRNAs identified in this study have significant potential as noninvasive markers of boar semen motility. Meanwhile, the results of the enrichment analysis provide novel insights into the mechanisms underlying the regulation of semen motility.
MeSH term(s)	Swine ; Male ; Animals ; Female ; Semen/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Exosomes/genetics ; Exosomes/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Sperm Motility ; Sequence Analysis, RNA/veterinary
Chemical Substances	MicroRNAs ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-)
Language	English
Publishing date	2023-12-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2023.11.028
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Article ; Online: Metabolomics-Based Analyses of Dynamic Changes in Flavonoid Profiles in the Black Mulberry Winemaking Process

Qin, Yanan / Xu, Haotian / Chen, Ya / Lei, Jing / Sun, Jingshuai / Zhao, Yan / Lian, Weijia / Zhang, Minwei

Foods. 2023 May 31, v. 12, no. 11

2023

Abstract: To overcome the fruit’s perishability, mulberry wine has been developed as a method of preservation. However, dynamic changes in metabolites during mulberry wine fermentation have not been reported yet. In the present investigation, UHPLC-QE-MS/MS ... ...

Abstract	To overcome the fruit’s perishability, mulberry wine has been developed as a method of preservation. However, dynamic changes in metabolites during mulberry wine fermentation have not been reported yet. In the present investigation, UHPLC-QE-MS/MS coupled with multivariate statistical analyses was employed to scrutinize the metabolic profiles, particularly the flavonoid profiles, throughout the process of vinification. In general, the major differential metabolites encompassed organic heterocyclic compounds, amino acids, phenylpropanoids, aromatic compounds, and carbohydrates. The contents of total sugar and alcohol play a primary role that drove the composition of amino acids, polyphenol, aromatic compound, and organic acid metabolites based on the Mantel test. Importantly, among the flavonoids, abundant in mulberry fruit, luteolin, luteolin-7-O-glucoside, (−)-epiafzelechin, eriodictyol, kaempferol, and quercetin were identified as the differential metabolic markers during blackberry wine fermentation and ripening. Flavonoid, flavone and flavonol biosynthesis were also identified to be the major metabolic pathways of flavonoids in 96 metabolic pathways. These results will provide new information on the dynamic changes in flavonoid profiles during black mulberry winemaking.
Keywords	Morus nigra ; biosynthesis ; blackberries ; fermentation ; fruits ; kaempferol ; luteolin ; metabolites ; mulberries ; phenylpropanoids ; polyphenols ; quercetin ; sugars ; winemaking ; wines
Language	English
Dates of publication	2023-0531
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12112221
Database	NAL-Catalogue (AGRICOLA)

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Article: Metabolomic analysis reveals spermatozoa and seminal plasma differences between Duroc and Liang guang Small-spotted pig.

Li, Zhili / Sun, Jingshuai / Li, Kebiao / Qin, Jiali / Sun, Yanmei / Zeng, Jianhua / El-Ashram, Saeed / Zhao, Yunxiang

Frontiers in veterinary science

2023 Volume 9, Page(s) 1078928

Abstract: The Liang guang Small-spotted pig is a well-known Chinese indigenous pig that is valued for its exceptional meat quality. However, the Liang guang Small-spotted pig has a lower semen storage capacity, shorter storage time and worse semen quality compared ...

Abstract	The Liang guang Small-spotted pig is a well-known Chinese indigenous pig that is valued for its exceptional meat quality. However, the Liang guang Small-spotted pig has a lower semen storage capacity, shorter storage time and worse semen quality compared to Duroc. Pig sperm used for artificial insemination (AI) loses part of vitality and quality when being stored in commercial solutions. Serious vitality losses and short shelf life of the semen are particularly prominent in Liang guang Small-spotted pig. In this study, the metabolites in seminal plasma and spermatozoa of Duroc and Liang guang Small-spotted pigs were identified using UHPLC-Q-TOF/MS technology. The findings indicated forty distinct metabolites concentrating on energy metabolic substrates and antioxidant capacity in Liang guang Small-spotted pig and Duroc seminal plasma, including D-Fructose, succinate, 2-dehydro-3-deoxy-d-gluconate, alanine betaine, citrate, carnitine, acetylcarnitine and so on. Seventeen different metabolites were explored, with a focus on glycerophospholipid metabolism in Liang guang Small-spotted pig and Duroc spermatozoa, primarily including glycerol 3-phosphate, acetylcarnitine, phosphatidylcholine (PC) 16:0/16:0, palmitoyl sphingomyelin, acetylcholine, choline, glycerophosphocholine, betaine, L-carnitine, creatinine and others. This study reveals the metabolite profile of spermatozoa and seminal plasma among different pig breeds and might be valuable for understanding the mechanisms that lead to sperm storage capacity. Metabolites involved in energy metabolism, antioxidant capacity and glycerophospholipid metabolism might be key to the poor sperm storage capacity in Liang guang Small-spotted pig.
Language	English
Publishing date	2023-01-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2834243-4
ISSN	2297-1769
ISSN	2297-1769
DOI	10.3389/fvets.2022.1078928
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Article: Metabolomics-Based Analyses of Dynamic Changes in Flavonoid Profiles in the Black Mulberry Winemaking Process.

Qin, Yanan / Xu, Haotian / Chen, Ya / Lei, Jing / Sun, Jingshuai / Zhao, Yan / Lian, Weijia / Zhang, Minwei

Foods (Basel, Switzerland)

2023 Volume 12, Issue 11

Abstract: To overcome the fruit's perishability, mulberry wine has been developed as a method of preservation. However, dynamic changes in metabolites during mulberry wine fermentation have not been reported yet. In the present investigation, UHPLC-QE-MS/MS ... ...

Abstract	To overcome the fruit's perishability, mulberry wine has been developed as a method of preservation. However, dynamic changes in metabolites during mulberry wine fermentation have not been reported yet. In the present investigation, UHPLC-QE-MS/MS coupled with multivariate statistical analyses was employed to scrutinize the metabolic profiles, particularly the flavonoid profiles, throughout the process of vinification. In general, the major differential metabolites encompassed organic heterocyclic compounds, amino acids, phenylpropanoids, aromatic compounds, and carbohydrates. The contents of total sugar and alcohol play a primary role that drove the composition of amino acids, polyphenol, aromatic compound, and organic acid metabolites based on the Mantel test. Importantly, among the flavonoids, abundant in mulberry fruit, luteolin, luteolin-7-O-glucoside, (-)-epiafzelechin, eriodictyol, kaempferol, and quercetin were identified as the differential metabolic markers during blackberry wine fermentation and ripening. Flavonoid, flavone and flavonol biosynthesis were also identified to be the major metabolic pathways of flavonoids in 96 metabolic pathways. These results will provide new information on the dynamic changes in flavonoid profiles during black mulberry winemaking.
Language	English
Publishing date	2023-05-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12112221
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Article: Small RNA expression patterns in seminal plasma exosomes isolated from semen containing spermatozoa with cytoplasmic droplets versus regular exosomes in boar semen

Sun, Jingshuai / Zhao, Yunxiang / He, Jian / Zhou, Qingbin / El-Ashram, Saeed / Yuan, Sheng / Chi, Shihong / Qin, Jiali / Huang, Zongyang / Ye, Manqing / Huang, Shujian / Li, Zhili

Theriogenology. 2021 Dec., v. 176

2021

Abstract: Multiple physiological pathways are controlled by exosomes. Exosomes may be found in seminal plasma where they carry functional molecules to the sperm, such as microRNAs (miRNAs). Sperm cytoplasmic droplets (CDs) are remnants of cytoplasm, and their ... ...

Abstract	Multiple physiological pathways are controlled by exosomes. Exosomes may be found in seminal plasma where they carry functional molecules to the sperm, such as microRNAs (miRNAs). Sperm cytoplasmic droplets (CDs) are remnants of cytoplasm, and their migration is a morphological characteristic of epididymal maturation. However, miRNA expression patterns in seminal plasma exosomes found in semen containing spermatozoa with CDs versus regular exosomes in boar semen have not been examined. In this study, seminal plasma exosomes were isolated from semen containing spermatozoa with CDs and miRNA expression profiles were analyzed. A total of 348 known and 206 new miRNAs were identified. Sixteen miRNAs were significantly differentially expressed. Of these, 13 miRNAs (ssc-miR-101, ssc-miR-148a-5p, ssc-miR-184, ssc-miR-202–3p, ssc-miR-221–5p, ssc-miR-2483, ssc-miR-29a-3p, ssc-miR-29c, ssc-miR-31, ssc-miR-362, ssc-miR-500–5p, ssc-miR-542–3p, and ssc-miR-769–5p) were significantly upregulated, whereas three miRNAs (ssc-miR-1249, ssc-miR-155–5p, and ssc-miR-296–5p) were significantly downregulated. GO and KEGG pathway analyses showed that these targeted genes were enriched for functions such as metabolic process, reproductive process, proteasome, ubiquitin mediated proteolysis, and oxidative phosphorylation. Therefore, seminal plasma exosomes are predicted to play a key role in the regulation of sperm CDs.
Keywords	animal reproduction ; boars ; epididymis ; exosomes ; microRNA ; oxidative phosphorylation ; proteasome endopeptidase complex ; proteolysis ; seminal plasma ; spermatozoa ; ubiquitin
Language	English
Dates of publication	2021-12
Size	p. 233-243.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2021.09.031
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Genetic variation analysis of the sigma B protein gene of novel duck reovirus in southeastern China from 2011 to 2020

Zhang, Wandi / Wang, Meihong / Yuan, Sheng / Li, Wenfeng / Huang, Jun / Sun, Jingshuai / Huang, Zongyang / Su, Langju / Yee, Manqing / Zhang, Xuelian / Huang, Shujian / El-Ashram, Saeed / Li, Zhili

Journal of Virological Methods. 2022 May, v. 303 p.114479-

2022

Abstract: The novel duck reovirus (NDRV) disease first appeared in China in 2011. Infected ducks may be of various ages and breeds. Sigma B protein, which is the key component of NDRV's outer capsid, may trigger group-specific neutralizing antibodies linked to ... ...

Abstract	The novel duck reovirus (NDRV) disease first appeared in China in 2011. Infected ducks may be of various ages and breeds. Sigma B protein, which is the key component of NDRV's outer capsid, may trigger group-specific neutralizing antibodies linked to NDRV infection, pathogenicity, and immune defense. The sigma B protein gene of fourteen NDRV field strains was amplified by RT-PCR and cloned into the pMD-18 T vector for sequencing to examine the genetic variation in sigma B proteins of NDRVs in southeastern China between 2011 and 2020. The sigma B protein gene of the fourteen NDRV southeastern strains included in this analysis had 96.3 %–99.8 % nucleotide, and 96.2 %–99.7 % deduced amino acid sequence homology. Phylogenetic analysis revealed that the fourteen southeastern strains belonged to a well-supported lineage that included NDRV and Muscovy duck reovirus (MDRV) strains. However, Avian reovirus (ARV) formed a distinct genetic lineage in the gene tree. The sigma B protein gene sequences of NDRV strains found in southeastern China are substantially conserved, according to these findings. There is no significant geographical difference between NDRV southeastern strains and DRV strains in other regions of China. Our findings will add to the molecular epidemiological picture of NDRV strain spread in southeastern China between 2011 and 2020, laying the groundwork for potential in-depth research on vaccine collection and comprehensive prevention.
Keywords	Avian orthoreovirus ; Cairina moschata ; amino acid sequences ; capsid ; ducks ; genes ; genetic variation ; geographical variation ; immune response ; pathogenicity ; phylogeny ; sequence homology ; vaccines ; China ; Novel duck reovirus (NDRV) ; Phylogenetic analysis ; Sigma B protein gene
Language	English
Dates of publication	2022-05
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2022.114479
Database	NAL-Catalogue (AGRICOLA)

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Article: Difference analysis of intestinal microbiota and metabolites in piglets of different breeds exposed to porcine epidemic diarrhea virus infection.

Li, Zhili / Zhang, Wandi / Su, Langju / Huang, Zongyang / Zhang, Weichao / Ma, Liangliang / Sun, Jingshuai / Guo, Jinyue / Wen, Feng / Mei, Kun / El-Ashram, Saeed / Huang, Shujian / Zhao, Yunxiang

Frontiers in microbiology

2022 Volume 13, Page(s) 990642

Abstract: The gut microbial composition of the Luchuan (LC) piglet, one of China's native breeds, has rarely been studied, especially when compared to other breeds. This study developed a porcine epidemic diarrhea virus (PEDV) infection model in LC and Largewhite ( ...

Abstract	The gut microbial composition of the Luchuan (LC) piglet, one of China's native breeds, has rarely been studied, especially when compared to other breeds. This study developed a porcine epidemic diarrhea virus (PEDV) infection model in LC and Largewhite (LW) piglets, and analyzed the patterns and differences of intestinal microbial communities and metabolites in piglets of these two breeds after infection. The diarrhea score, survival time, and distribution of viral antigens in the intestine of piglets infected with PEDV differed among breeds, with the jejunal immunohistochemistry score of LW piglets being significantly higher than that of LC piglets (
Language	English
Publishing date	2022-11-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2022.990642
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Article ; Online: Genetic variation analysis of the sigma B protein gene of novel duck reovirus in southeastern China from 2011 to 2020.

Zhang, Wandi / Wang, Meihong / Yuan, Sheng / Li, Wenfeng / Huang, Jun / Sun, Jingshuai / Huang, Zongyang / Su, Langju / Yee, Manqing / Zhang, Xuelian / Huang, Shujian / El-Ashram, Saeed / Li, Zhili

Journal of virological methods

2022 Volume 303, Page(s) 114479

Abstract	The novel duck reovirus (NDRV) disease first appeared in China in 2011. Infected ducks may be of various ages and breeds. Sigma B protein, which is the key component of NDRV's outer capsid, may trigger group-specific neutralizing antibodies linked to NDRV infection, pathogenicity, and immune defense. The sigma B protein gene of fourteen NDRV field strains was amplified by RT-PCR and cloned into the pMD-18 T vector for sequencing to examine the genetic variation in sigma B proteins of NDRVs in southeastern China between 2011 and 2020. The sigma B protein gene of the fourteen NDRV southeastern strains included in this analysis had 96.3 %-99.8 % nucleotide, and 96.2 %-99.7 % deduced amino acid sequence homology. Phylogenetic analysis revealed that the fourteen southeastern strains belonged to a well-supported lineage that included NDRV and Muscovy duck reovirus (MDRV) strains. However, Avian reovirus (ARV) formed a distinct genetic lineage in the gene tree. The sigma B protein gene sequences of NDRV strains found in southeastern China are substantially conserved, according to these findings. There is no significant geographical difference between NDRV southeastern strains and DRV strains in other regions of China. Our findings will add to the molecular epidemiological picture of NDRV strain spread in southeastern China between 2011 and 2020, laying the groundwork for potential in-depth research on vaccine collection and comprehensive prevention.
MeSH term(s)	Animals ; China/epidemiology ; Genetic Variation ; Orthoreovirus, Avian/genetics ; Phylogeny ; Poultry Diseases ; Reoviridae Infections/epidemiology ; Reoviridae Infections/veterinary
Language	English
Publishing date	2022-01-31
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2022.114479
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