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Article ; Online: Perfluorooctanoic acid (PFOA) exposure induces renal filtration and reabsorption disorders via down-regulation of aquaporins.

Wang, Li / Sun, Weiqiang / Ma, Xinzhuang / Griffin, Nathan / Liu, Hui

2023 Volume 392, Page(s) 22–35

Abstract: Perfluorooctanoic acid (PFOA) exposure is associated with kidney dysfunction, however the exact mechanisms by which PFOA induces nephrotoxicity and the specific involvement of aquaporins (AQPs) in kidney tissue remains unclear. In this study, adult male ... ...

Abstract	Perfluorooctanoic acid (PFOA) exposure is associated with kidney dysfunction, however the exact mechanisms by which PFOA induces nephrotoxicity and the specific involvement of aquaporins (AQPs) in kidney tissue remains unclear. In this study, adult male Sprague-Dawley (SD) rats were exposed to PFOA by oral gavage for 28 days and compared with controls. Body weight, water intake and urine volume were recorded daily. At the end of the experiment, blood and kidney samples were collected, and serum urea, creatine and uric acid levels were assessed. The renal expression levels of water channel proteins AQP1, AQP3, AQP2 and p-AQP2 (Ser256) were observed by immunohistochemical staining, and the corresponding transcription levels were detected by Western blot and qRT-PCR. The results showed that PFOA exposure inhibited weight gain and increased water intake, urine volume, kidney weight and renal visceral index. PASM staining and transmission electron microscopy revealed pathological thickening of the glomerular capsule and basement membrane. Serum urea levels were increased, while serum creatine levels were decreased compared to controls. Additionally, the expression levels of AQP1, AQP3, AQP2 and p-AQP2 in kidney tissues were decreased, and the phosphorylation of AQP2 at Ser256 was inhibited. In conclusion, we demonstrate that PFOA exposure can damage the renal filtration barrier and reduce the expression level of AQPs in renal tissues, leading to renal filtration and reabsorption disorders.
MeSH term(s)	Rats ; Animals ; Male ; Aquaporin 2/genetics ; Aquaporin 2/metabolism ; Down-Regulation ; Creatine/metabolism ; Rats, Sprague-Dawley ; Kidney/metabolism ; Urea/metabolism ; Caprylates ; Fluorocarbons
Chemical Substances	perfluorooctanoic acid (947VD76D3L) ; Aquaporin 2 ; Creatine (MU72812GK0) ; Urea (8W8T17847W) ; Caprylates ; Fluorocarbons
Language	English
Publishing date	2023-12-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	433788-8
ISSN	1879-3169 ; 0378-4274
ISSN (online)	1879-3169
ISSN	0378-4274
DOI	10.1016/j.toxlet.2023.12.003
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Book ; Online: Twotier -- A Layered Analysis of Backbone Members in a Moderate Sized Community Sports Organization

Wang, Qingran / Yu, Jia / Ding, Mengjun / Sun, Weiqiang

2023

Abstract: Backbone members are recognized as essential parts of an organization, yet their role and mechanisms of functioning in networks are not fully understood. In this paper, we propose a new framework called Twotier to analyze the evolution of community ... ...

Abstract	Backbone members are recognized as essential parts of an organization, yet their role and mechanisms of functioning in networks are not fully understood. In this paper, we propose a new framework called Twotier to analyze the evolution of community sports organizations (CSOs) and the role of backbone members. Tier-one establishes a dynamic user interaction network based on grouping relationships, and weighted k-shell decomposition is used to select backbone members. We perform community detection and capture the evolution of two separate sub-networks: one formed by backbone members and the other formed by other members. In Tier-two, the sub-networks are abstracted, revealing a core-periphery structure in the organization where backbone members serve as bridges connecting all parts of the network. Our findings suggest that relying on backbone members can keep newcomers actively involved in rewarding activities, while non-rewarding activities solidify relations between backbone members.
Keywords	Computer Science - Social and Information Networks
Publishing date	2023-07-09
Publishing country	us
Document type	Book ; Online
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Article ; Online: PFOA exposure induces aberrant glucose and lipid metabolism in the rat liver through the AMPK/mTOR pathway

Zhang, Xuemin / Ren, Xijuan / Sun, Weiqiang / Griffin, Nathan / Wang, Li / Liu, Hui

Toxicology. 2023 July, v. 493 p.153551-

2023

Abstract: Perfluorooctanoic acid (PFOA) is the most prominent member of a widely utilized family of compounds named Perfluoroalkyl substances (PFASs). Initially produced for use in both industrial and consumer applications, it has since been recognized that PFASs ... ...

Abstract	Perfluorooctanoic acid (PFOA) is the most prominent member of a widely utilized family of compounds named Perfluoroalkyl substances (PFASs). Initially produced for use in both industrial and consumer applications, it has since been recognized that PFASs are extremely persistent in the environment where they have been characterized as persistent organic pollutants (POPs). While previous studies have demonstrated that PFOA may induce disorders of lipid and carbohydrate metabolism, the precise mechanisms by which PFOA produces this phenotype and the involvement of downstream AMPK/mTOR pathways remains unclear. In this study, male rats were exposed to 1.25, 5 and 20 mg PFOA/kg body weight/day for 28 days by oral gavage. After 28 days, blood was collected and tested for serum biochemical indicators and livers were removed and weighed. To investigate aberrant metabolism in rats exposed to PFOA, livers were analyzed by performing LC-MS/MS untargeted metabolomics, quantitative real-time PCR, western blotting, immunohistochemical staining was also performed on exposed tissues. Our results showed that exposure to PFOA induced liver damage, increased the expression of glucose and lipid related biochemical indexes in liver and serum, and altered the expression levels of AMPK/mTOR pathway related genes and proteins. In summary, this study clarifies the mechanisms responsible for PFOA toxicity in the liver of exposed animals.
Keywords	blood serum ; body weight ; carbohydrate metabolism ; glucose ; immunohistochemistry ; lipid metabolism ; lipids ; liver ; males ; metabolomics ; perfluorocarbons ; perfluorooctanoic acid ; phenotype ; quantitative polymerase chain reaction ; rats ; toxicity ; toxicology ; Perfluorooctanoic acid (PFOA) ; Liver injury ; Metabolism ; AMPK/mTOR
Language	English
Dates of publication	2023-07
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	184557-3
ISSN	1879-3185 ; 0300-483X
ISSN (online)	1879-3185
ISSN	0300-483X
DOI	10.1016/j.tox.2023.153551
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Zs.A 888: Show issues

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Article: [Effect of perfluorooctanoic acid on lipid accumulation in rat liver cells BRL-3A and its possible mechanism].

Sun, Weiqiang / Ma, Xinzhuang / Zhou, Yongbing / Wang, Li / Liu, Hui

Wei sheng yan jiu = Journal of hygiene research

2022 Volume 51, Issue 1, Page(s) 107–112

Abstract: Objective: To investigate the effect of perfluorooctanoic acid on rat hepatocytes BRL-3 A cell viability and the expression of transcription factor nuclear factor erythroid 2-related factor 2(Nrf2) and arginosuccinate synthase(Ass1).: Methods: Rat ... ...

Abstract	Objective: To investigate the effect of perfluorooctanoic acid on rat hepatocytes BRL-3 A cell viability and the expression of transcription factor nuclear factor erythroid 2-related factor 2(Nrf2) and arginosuccinate synthase(Ass1). Methods: Rat hepatocytes BRL-3 A were cultured and divided into control group(0 μmol/L PFOA), low-dose group(6.25 μmol/L PFOA), and medium-dose group(25 μmol/L PFOA), high-dose group(100 μmol/L PFOA). After 48 hours, cell viability was detected by MTT, ROS content was detected by free radical indicator H_2DCFDA, enzyme activity related to oxidative stress was detected by the kit, Nrf2 and Ass1 protein expression level was detected by Western blot and immunocytochemistry(ICC). Results: Compared with the control group, with the increase of the PFOA concentration, the cell viability of the middle and high dose groups had a downward trend, but there was no statistical significance(P>0.05). The intracellular ROS content increased, among which in the middle and high dose groups significantly increased(P<0.05), and the average fluorescence intensity was(5417.66±161.09) and(5725.50±166.83), respectively. Compared with the control group, the content of intracellular TG, TC and MDA in the low and medium dose groups did not change significantly, and the content of TG, TC and MDA in the high dose group was significantly increased(P<0.05), which was(0.21±0.05) mmol/L, (14.5±6.07) mmol/L and(1.23±0.33) nmol/mL, respectively. According to the ICC and Western blot result, the expression level of Nrf2 protein increased significantly in the high-dose group(P<0.05), and the expression level of Ass1 protein increased significantly in the low-dose group(P<0.05). Conclusion: Exposure to a certain dose of PFOA can lead to the accumulation of ROS in BRL-3 A cells. Nrf2 and Ass1 can play a certain role in eliminating ROS and ammonia detoxification by increasing their expression under the oxidative damage of rat liver cells caused by PFOA.
MeSH term(s)	Animals ; Caprylates/toxicity ; Fluorocarbons/toxicity ; Hepatocytes ; Liver ; Rats
Chemical Substances	Caprylates ; Fluorocarbons ; perfluorooctanoic acid (947VD76D3L)
Language	Chinese
Publishing date	2022-01-10
Publishing country	China
Document type	Journal Article
ZDB-ID	1050909-4
ISSN	1000-8020
ISSN	1000-8020
DOI	10.19813/j.cnki.weishengyanjiu.2022.01.018
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Zs.A 4878: Show issues

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Article ; Online: PFOA exposure induces aberrant glucose and lipid metabolism in the rat liver through the AMPK/mTOR pathway.

Zhang, Xuemin / Ren, Xijuan / Sun, Weiqiang / Griffin, Nathan / Wang, Li / Liu, Hui

Toxicology

2023 Volume 493, Page(s) 153551

Abstract	Perfluorooctanoic acid (PFOA) is the most prominent member of a widely utilized family of compounds named Perfluoroalkyl substances (PFASs). Initially produced for use in both industrial and consumer applications, it has since been recognized that PFASs are extremely persistent in the environment where they have been characterized as persistent organic pollutants (POPs). While previous studies have demonstrated that PFOA may induce disorders of lipid and carbohydrate metabolism, the precise mechanisms by which PFOA produces this phenotype and the involvement of downstream AMPK/mTOR pathways remains unclear. In this study, male rats were exposed to 1.25, 5 and 20 mg PFOA/kg body weight/day for 28 days by oral gavage. After 28 days, blood was collected and tested for serum biochemical indicators and livers were removed and weighed. To investigate aberrant metabolism in rats exposed to PFOA, livers were analyzed by performing LC-MS/MS untargeted metabolomics, quantitative real-time PCR, western blotting, immunohistochemical staining was also performed on exposed tissues. Our results showed that exposure to PFOA induced liver damage, increased the expression of glucose and lipid related biochemical indexes in liver and serum, and altered the expression levels of AMPK/mTOR pathway related genes and proteins. In summary, this study clarifies the mechanisms responsible for PFOA toxicity in the liver of exposed animals.
MeSH term(s)	Male ; Rats ; Animals ; Lipid Metabolism ; AMP-Activated Protein Kinases/metabolism ; Glucose/metabolism ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Caprylates/toxicity ; Liver/metabolism ; Fluorocarbons/toxicity ; Fluorocarbons/metabolism ; TOR Serine-Threonine Kinases/metabolism
Chemical Substances	perfluorooctanoic acid (947VD76D3L) ; AMP-Activated Protein Kinases (EC 2.7.11.31) ; Glucose (IY9XDZ35W2) ; Caprylates ; Fluorocarbons ; TOR Serine-Threonine Kinases (EC 2.7.11.1) ; mTOR protein, rat (EC 2.7.1.1)
Language	English
Publishing date	2023-05-24
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	184557-3
ISSN	1879-3185 ; 0300-483X
ISSN (online)	1879-3185
ISSN	0300-483X
DOI	10.1016/j.tox.2023.153551
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Zs.A 888: Show issues

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Article ; Online: Comparison of the efficacy and safety of antibiotic treatment and appendectomy for acute uncomplicated appendicitis: a systematic review and meta-analysis.

Xu, Hongxia / Yang, Shaohui / Xing, Jiankun / Wang, Yan / Sun, Weiqiang / Rong, Lingyan / Liu, Huihui

BMC surgery

2023 Volume 23, Issue 1, Page(s) 208

Abstract: Objective: This meta-analysis aimed to compare the efficacy and safety of antibiotic treatment and appendectomy for acute uncomplicated appendicitis.: Methods: We searched the randomized controlled studies (RCTs) comparing appendectomy with ... ...

Abstract	Objective: This meta-analysis aimed to compare the efficacy and safety of antibiotic treatment and appendectomy for acute uncomplicated appendicitis. Methods: We searched the randomized controlled studies (RCTs) comparing appendectomy with antibiotic treatment for uncomplicated acute appendicitis in the electronic database including Pubmed, Embase, Cochrane, Web of Science, CNKI, VIP, and WanFang. The primary outcomes included complication-free treatment success at 1 year, complications, surgical complications, and the complicated appendicitis rates. Secondary outcomes included negative appendicitis, length of hospital stay, the quality of life at 1 month, and the impact of an appendicolith on antibiotic therapy. Results: Twelve randomized controlled studies were included. Compared with surgery group, the antibiotic group decreased the complication-free treatment success at 1 year (RR 0.81; 95% CI 0.73-0.91; z = 3.65; p = 0.000). Statistically significance was existed between antibiotic group and surgical group with both surgical types(open and laparoscopic) (RR 0.43; 95% CI 0.31-0.58; z = 5.36; p = 0.000), while no between the antibiotic treatment and laparoscopic surgery (RR 0.72; 95% CI 0.41-1.24; z = 1.19; p = 0.236). There was no statistically significant differences between two groups of surgical complications (RR 1.38; 95% CI 0.70-2.73; z = 0.93; p = 0.353), the complicated appendicitis rate (RR 0.71; 95% CI 0.36-1.42; z = 0.96; p = 0.338), negative appendectomy rate (RR 1.11; 95% CI 0.69-1.79; z = 0.43; p = 0.670), duration of hospital stay (SMD 0.08; 95%CI -0.11-0.27; z = 0.80; p = 0.422), and quality of life at 1 month (SMD 0.09; 95%CI -0.03-0.20; z = 1.53; p = 0.127). However, in the antibiotic treatment group, appendicolith rates were statistically higher in those whose symptoms did not improve (RR 2.94; 95% CI 1.28-6.74; z = 2.55; p = 0.011). Conclusions: Although the cure rate of antibiotics is lower than surgery, antibiotic treatment is still a reasonable option for patients with uncomplicated acute appendicitis who do not want surgery without having to worry about complications or complicating the original illness.
MeSH term(s)	Humans ; Appendectomy ; Appendicitis/drug therapy ; Appendicitis/surgery ; Anti-Bacterial Agents/therapeutic use ; Treatment Outcome ; Acute Disease ; Length of Stay
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2023-07-24
Publishing country	England
Document type	Meta-Analysis ; Systematic Review ; Journal Article
ZDB-ID	2050442-1
ISSN	1471-2482 ; 1471-2482
ISSN (online)	1471-2482
ISSN	1471-2482
DOI	10.1186/s12893-023-02108-1
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Article ; Online: Exposure to PFOA and its novel analogs disrupts lipid metabolism in zebrafish.

Sun, Weiqiang / Zhang, Xuemin / Qiao, Ying / Griffin, Nathan / Zhang, Hongxia / Wang, Li / Liu, Hui

Ecotoxicology and environmental safety

2023 Volume 259, Page(s) 115020

Abstract: Perfluorooctanoic acid (PFOA), a typical perfluoroalkyl group compound, has received worldwide attention due to its significant environmental toxicity. Following regulatory bans on the production and emission of PFOA, concerns have been raised about the ... ...

Abstract	Perfluorooctanoic acid (PFOA), a typical perfluoroalkyl group compound, has received worldwide attention due to its significant environmental toxicity. Following regulatory bans on the production and emission of PFOA, concerns have been raised about the potential health risks and the safety of novel perfluoroalkyl analogues. HFPO-DA (trade name Gen-X) and HFPO-TA are two perfluoroalkyl analogues known to be bioaccumulative, whose level of toxicity and whether they are safe alternatives to PFOA remain unclear. In the following study, the physiological and metabolic effects of exposure to PFOA and its novel analogues were explored in zebrafish using 1/3 LC
MeSH term(s)	Animals ; Zebrafish/genetics ; Lipid Metabolism ; Fluorocarbons/toxicity ; Caprylates/toxicity
Chemical Substances	perfluorooctanoic acid (947VD76D3L) ; ammonium 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy)-propanoate ; Fluorocarbons ; Caprylates
Language	English
Publishing date	2023-05-17
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	436536-7
ISSN	1090-2414 ; 0147-6513
ISSN (online)	1090-2414
ISSN	0147-6513
DOI	10.1016/j.ecoenv.2023.115020
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Zs.A 1418: Show issues

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Article: Perfluorooctanoic acid

Wang, Li / Zhou, Yongbing / Ma, Xinzhuang / Sun, Weiqiang / Liu, Hui

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences

2022 Volume 47, Issue 1, Page(s) 18–25

Abstract: Objectives: Perfluorooctanoic acid (PFOA) can cause lipid metabolism disorders in animal body and affect the lipolysis and synthesis of fatty acids. Peroxisome proliferators-activated receptor (PPAR) plays an extremely important role in this process. ... ...

Title translation	全氟辛酸对SD大鼠肝脂质代谢紊乱及脂肪酸代谢相关蛋白表达的影响.
Abstract	Objectives: Perfluorooctanoic acid (PFOA) can cause lipid metabolism disorders in animal body and affect the lipolysis and synthesis of fatty acids. Peroxisome proliferators-activated receptor (PPAR) plays an extremely important role in this process. This study aims to explore the effects of PFOA on liver lipid metabolism disorders in Sprague Dewley (SD) rats and the expression of PPAR. Methods: A total of 40 male SD rats were randomly divided into 4 groups ( Results: After 14 days of PFOA exposure, the PAS staining positive particles in the cytoplasm and nucleus of SD rats in the medium and high dose groups were significantly reduced compared with the control group. The serum levels of LDLC and TC in the low-dose and middle-dose groups were significantly reduced compared with the control group (all Conclusions: PFOA exposure can cause lipid metabolism disorder and glycogen reduction in SD rat livers, which may be related to the activation of Sirt1 and inhibition of Ppar γ expression, leading to affecting the normal metabolism of fatty acids and promoting glycolysis.
MeSH term(s)	Animals ; Caprylates ; Fatty Acids/metabolism ; Fatty Acids/pharmacology ; Fluorocarbons ; Lipid Metabolism ; Lipid Metabolism Disorders/chemically induced ; Lipid Metabolism Disorders/metabolism ; Liver/metabolism ; Male ; PPAR gamma ; Rats ; Rats, Sprague-Dawley ; Sirtuin 1/metabolism
Chemical Substances	Caprylates ; Fatty Acids ; Fluorocarbons ; PPAR gamma ; perfluorooctanoic acid (947VD76D3L) ; Sirtuin 1 (EC 3.5.1.-)
Language	Chinese
Publishing date	2022-05-09
Publishing country	China
Document type	Journal Article
ZDB-ID	2168533-2
ISSN	1672-7347
ISSN	1672-7347
DOI	10.11817/j.issn.1672-7347.2022.210320
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Book ; Online: Understanding the power-law nature of participation in community sports organizations

Yu, Jia / Ding, Mengjun / Sun, Weiqiang / Hu, Weisheng / Wang, Huiru

2023

Abstract: The improvement of living standards and awareness of chronic diseases have increased the importance of community sports organizations in promoting the physical activity levels of the public. However, limited understanding of human behavior in this ... ...

Abstract	The improvement of living standards and awareness of chronic diseases have increased the importance of community sports organizations in promoting the physical activity levels of the public. However, limited understanding of human behavior in this context often leads to suboptimal resource utilization. In this study, we analyzed the participation behavior of 2,956 members with a time span of 6 years in a community sports organization. Our study reveals that, at the population level, the participation frequency in activities adheres to a power-law distribution. To understand the underlying mechanisms driving crowd participation, we introduce a novel behavioral model called HFBI (Habit-Formation and Behavioral Inertia), demonstrating a robust fit to the observed power-law distribution. The habit formation mechanism indicates that individuals who are more engaged are more likely to maintain participation, while the behavioral inertia mechanism suggests that individuals' willingness to participate in activities diminishes with their absences from activities. At the individual level, our analysis reveals a burst-quiet participation pattern, with bursts often commencing with incentive activities. We also find a power-law distribution in the intervals between individual participations. Our research offers valuable insights into the complex dynamics of human participation in community sports activity and provides a theoretical foundation to inform intervention design. Furthermore, the flexibility of our model enables its application to other data exhibiting power-law properties, broadening its potential impact beyond the realm of community sports.
Keywords	Computer Science - Social and Information Networks ; Physics - Physics and Society
Subject code	612 ; 306
Publishing date	2023-07-08
Publishing country	us
Document type	Book ; Online
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Article ; Online: Effects of acupuncture on expression of VEGF, PLA2 and PGE2 and extracellular matrix collagen and metabolic enzymes in the intervertebral disc of rats with cervical disc degeneration

Sun Wei-Qiang / Zhang Guang-Zhi

Journal of Hainan Medical University, Vol 25, Iss 17, Pp 29-

2019 Volume 34

Abstract: Objective: The aim of this study was to investigate the effects of probe puncture on the expression of VEGF, PLA2 and PGE2 and extracellular matrix collagen and metabolic enzymes in the intervertebral disc of rats with cervical disc degeneration. Methods: ...

Abstract	Objective: The aim of this study was to investigate the effects of probe puncture on the expression of VEGF, PLA2 and PGE2 and extracellular matrix collagen and metabolic enzymes in the intervertebral disc of rats with cervical disc degeneration. Methods: Rats were randomly assigned to the following three groups (n = 25 per group): sham operation group (cut neck and then suture), model group (treated by modeling), and acupuncture treatment group (continuous daily) 30 minutes, complete course of treatment consisted of 14 days, 2 days between two courses); mRNA expression levels of VEGF, PLA2 and PGE2 were analyzed by qRT-PCR; protein levels of VEGF, PLA2 and PGE2 proteins were determined by Western blotting; Immunohistochemical staining was used to detect type I and type II collagen; TNF-α, IL-1β, MMP-1 and MMP-3 levels were detected by enzyme-linked immunosorbent assay; TUNEL assay was used to evaluate acupuncture treatment for cervical disc degeneration The effect of apoptosis. Results: The expression levels of VEGF, PLA2 and PGE2 mRNA and protein in the model group were higher than those in the sham operation group, while the acupuncture treatment group reduced the expression levels of VEGF, PLA2 and PGE2 mRNA and protein in the model group (P<0.05). The type I collagen was positively correlated with disc degeneration, and type II collagen was negatively correlated with disc degeneration. In the model group (0.18±0.05, 0.11±0.03), the expression level of type I collagen was higher than that of the sham operation group (0.12±0.03), the expression level of type II collagen was decreased (0.19±0.04), and the acupuncture treatment group was able to restore the model. Collagen levels of group I (0.14±0.03) and type II (0.17±0.03) were different between the three groups (P<0.05). Compared with the sham operation group, the model group was TNF-α, IL-1β, The expression levels of MMP-1 and MMP-3 were increased, while the acupuncture treatment group reduced the expression levels of TNF-α, IL-1β, MMP-1 and MMP-3 in ...
Keywords	acupuncture ; cervical disc degeneration ; extracellular matrix collagen ; matrix metalloproteinase ; Medicine ; R
Subject code	630
Language	English
Publishing date	2019-09-01T00:00:00Z
Publisher	Editorial Board of Journal of Hainan Medical University
Document type	Article ; Online
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