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  1. Article: Effects of High-Forage Diets Containing Raw Flaxseeds or Soybean on In Vitro Ruminal Fermentation, Gas Emission, and Microbial Profile

    Sun, Xiao-Ge / Wang, Yue / Xie, Tian / Yang, Zhan-Tao / Wang, Ji-Dong / Zheng, Yu-Hui / Guo, Cheng / Zhang, Yan / Wang, Qian-Qian / Wang, Zhong-Han / Wang, Wei / Wang, Ya-Jing / Yang, Hong-Jian / Li, Sheng-Li

    Microorganisms. 2021 Nov. 05, v. 9, no. 11

    2021  

    Abstract: Lipid metabolism plays an important role in the energy economy of ruminants. However, its interactions of fat, rumen fermentation, gas emission, and microorganisms are not yet clear. This study evaluated the effect of adding raw oilseeds to high-forage ... ...

    Abstract Lipid metabolism plays an important role in the energy economy of ruminants. However, its interactions of fat, rumen fermentation, gas emission, and microorganisms are not yet clear. This study evaluated the effect of adding raw oilseeds to high-forage diets on in vitro ruminal fermentation, gas composition, and microbial profile. Three isoenergetic and isoproteic experimental diets were designed and used as fermentation substrate: control treatment (CON group) was the basal diet lacking oilseeds, the other two treatments were the basal diet supplemented by 100 g/kg dry matter (DM) raw whole soybean (S group) and 50 g/kg DM raw flaxseed (F group), respectively. Data showed that the acetate, butyrate, and total VFA concentration of culture fluids in the S group were lower (p < 0.05) than in the F group. There was a tendency to a higher level (p = 0.094) of propionate concentration in the F group compared with the other two groups. The gas production in the F group was higher (p < 0.05) than in the control group. There was a lower abundance of Sutterella (p < 0.05) and a greater abundance of Butyrivibrio (p < 0.05) in both of the two oilseed treatments. Methanobrevibacter (p = 0.078) in the F group was the lowest. Our results suggested that CH₄ emission could be inhibited with flaxseed supplementation by propionate production metabolism, biohydrogenation of unsaturated fatty acid (FA), and toxicity to Methanobrevibacter, while regarding soybean seed supplementation, the emission of CH₄ was more likely to be reduced through biohydrogenation of unsaturated FA modulated by Butyrivibrio.
    Keywords Butyrivibrio ; Methanobrevibacter ; Sutterella ; acetates ; biohydrogenation ; butyrates ; energy ; fermentation ; linseed ; propionic acid ; rumen fermentation ; soybeans ; toxicity ; unsaturated fatty acids
    Language English
    Dates of publication 2021-1105
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9112304
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Correction to: Expression profle of PU.1 in CD4

    Xiang, Nan / Fang, Xuan / Sun, Xiao-Ge / Zhou, Ying-Bo / Ma, Yan / Li, Xiang-Pei / Wang, Guo-Sheng / Tao, Jin-Hui / Li, Xiao-Mei

    Clinical and experimental medicine

    2021  Volume 22, Issue 2, Page(s) 325

    Language English
    Publishing date 2021-06-02
    Publishing country Italy
    Document type Published Erratum
    ZDB-ID 2053018-3
    ISSN 1591-9528 ; 1591-8890
    ISSN (online) 1591-9528
    ISSN 1591-8890
    DOI 10.1007/s10238-021-00731-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5481: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
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  3. Article ; Online: Expression profile of PU.1 in CD4

    Xiang, Nan / Fang, Xuan / Sun, Xiao-Ge / Zhou, Ying-Bo / Ma, Yan / Zhu, Chen / Li, Xiang-Pei / Wang, Guo-Sheng / Tao, Jin-Hui / Li, Xiao-Mei

    Clinical and experimental medicine

    2021  Volume 21, Issue 4, Page(s) 621–632

    Abstract: Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease with complex genetic predisposing factors involved. PU.1 is an important member of the ETS transcription factors family which has diverse functions such as regulating the ... ...

    Abstract Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease with complex genetic predisposing factors involved. PU.1 is an important member of the ETS transcription factors family which has diverse functions such as regulating the proliferation, differentiation of immune cells and multiple inflammatory cytokines. Previous studies preliminary explored the relation between PU.1 and SLE. To further explain the potential role of PU.1 in the pathogenesis of SLE, 40 SLE patients and 20 age-sex matched healthy controls (HC) were recruited in this study. Flow cytometry was used to test the percentages of CD4
    MeSH term(s) CD4-Positive T-Lymphocytes ; Cytokines ; Flow Cytometry ; Humans ; Leukocytes, Mononuclear ; Lupus Erythematosus, Systemic ; Proto-Oncogene Proteins ; T-Lymphocytes ; Trans-Activators
    Chemical Substances Cytokines ; Proto-Oncogene Proteins ; Trans-Activators ; proto-oncogene protein Spi-1
    Language English
    Publishing date 2021-05-08
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 2053018-3
    ISSN 1591-9528 ; 1591-8890
    ISSN (online) 1591-9528
    ISSN 1591-8890
    DOI 10.1007/s10238-021-00717-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5481: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Effects of High-Forage Diets Containing Raw Flaxseeds or Soybean on In Vitro Ruminal Fermentation, Gas Emission, and Microbial Profile.

    Sun, Xiao-Ge / Wang, Yue / Xie, Tian / Yang, Zhan-Tao / Wang, Ji-Dong / Zheng, Yu-Hui / Guo, Cheng / Zhang, Yan / Wang, Qian-Qian / Wang, Zhong-Han / Wang, Wei / Wang, Ya-Jing / Yang, Hong-Jian / Li, Sheng-Li

    Microorganisms

    2021  Volume 9, Issue 11

    Abstract: Lipid metabolism plays an important role in the energy economy of ruminants. However, its interactions of fat, rumen fermentation, gas emission, and microorganisms are not yet clear. This study evaluated the effect of adding raw oilseeds to high-forage ... ...

    Abstract Lipid metabolism plays an important role in the energy economy of ruminants. However, its interactions of fat, rumen fermentation, gas emission, and microorganisms are not yet clear. This study evaluated the effect of adding raw oilseeds to high-forage diets on in vitro ruminal fermentation, gas composition, and microbial profile. Three isoenergetic and isoproteic experimental diets were designed and used as fermentation substrate: control treatment (CON group) was the basal diet lacking oilseeds, the other two treatments were the basal diet supplemented by 100 g/kg dry matter (DM) raw whole soybean (S group) and 50 g/kg DM raw flaxseed (F group), respectively. Data showed that the acetate, butyrate, and total VFA concentration of culture fluids in the S group were lower (
    Language English
    Publishing date 2021-11-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9112304
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Effects of high-forage diets containing raw flaxseeds or soybean on in vitro ruminal fermentation, gas emission, and microbial profile

    Sun, Xiao Ge / Wang, Yue / Xie, Tian / Yang, Zhan Tao / Wang, Ji Dong / Zheng, Yu Hui / Guo, Cheng / Zhang, Yan / Wang, Qian Qian / Wang, Zhong Han / Wang, Wei / Wang, Ya Jing / Yang, Hong Jian / Li, Sheng Li

    Microorganisms

    2021  Volume 9, Issue 11

    Abstract: Lipid metabolism plays an important role in the energy economy of ruminants. However, its interactions of fat, rumen fermentation, gas emission, and microorganisms are not yet clear. This study evaluated the effect of adding raw oilseeds to high-forage ... ...

    Abstract Lipid metabolism plays an important role in the energy economy of ruminants. However, its interactions of fat, rumen fermentation, gas emission, and microorganisms are not yet clear. This study evaluated the effect of adding raw oilseeds to high-forage diets on in vitro ruminal fermentation, gas composition, and microbial profile. Three isoenergetic and isoproteic experimental diets were designed and used as fermentation substrate: control treatment (CON group) was the basal diet lacking oilseeds, the other two treatments were the basal diet supplemented by 100 g/kg dry matter (DM) raw whole soybean (S group) and 50 g/kg DM raw flaxseed (F group), respectively. Data showed that the acetate, butyrate, and total VFA concentration of culture fluids in the S group were lower (p < 0.05) than in the F group. There was a tendency to a higher level (p = 0.094) of propionate concentration in the F group compared with the other two groups. The gas production in the F group was higher (p < 0.05) than in the control group. There was a lower abundance of Sutterella (p < 0.05) and a greater abundance of Butyrivibrio (p < 0.05) in both of the two oilseed treatments. Methanobrevibacter (p = 0.078) in the F group was the lowest. Our results suggested that CH4 emission could be inhibited with flaxseed supplementation by propionate production metabolism, biohydrogenation of unsaturated fatty acid (FA), and toxicity to Methanobrevibacter, while regarding soybean seed supplementation, the emission of CH4 was more likely to be reduced through biohydrogenation of unsaturated FA modulated by Butyrivibrio.
    Keywords CH emission ; High-forage diets ; Microbial communities ; Oilseed ; Volatile acid
    Subject code 660
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Negative Correlation Between miR-326 and Ets-1 in Regulatory T Cells from new-Onset SLE Patients.

    Sun, Xiao-Ge / Tao, Jin-Hui / Xiang, Nan / Li, Xiao-Mei / Wang, Guo-Sheng / Fang, Xuan / Dai, Chao / Zhang, Min / Chen, Zhu / Li, Xiang-Pei

    Inflammation

    2016  Volume 39, Issue 2, Page(s) 822–829

    Abstract: To analyze the relationship between miR-326 and Ets-1 mRNA levels in Treg cells and clinical manifestations in patients with SLE and explore the role of miR-326 and Ets-1 in the pathogenesis and activity of SLE. Twenty-five new-onset SLE patients without ...

    Abstract To analyze the relationship between miR-326 and Ets-1 mRNA levels in Treg cells and clinical manifestations in patients with SLE and explore the role of miR-326 and Ets-1 in the pathogenesis and activity of SLE. Twenty-five new-onset SLE patients without treatment, twenty-eight inactive SLE patients (SLEDA ≤ 4) and twenty-two healthy controls were included in the present study. Clinical data of SLE patients were recorded. Treg cells were purified by MACS from 20 ml peripheral blood, in which the quantity of miR-326 and Ets-1 mRNA were assessed by real-time PCR. Data were analyzed using SPSS Version 17.0. The nonparametric Mann-Whitney U test was used to compare the groups, The Spearman test was used for correlation analyses. Two-tailed p values <0.05 were considered statistically significant. 1.The level of miR-326 was significantly higher in Treg cells from SLE patients [1.98(0.592,6.148)] than that in healthy controls [0.921(0.345, 1.879)] (p = 0.032). The difference between new-onset SLE patients [6.192(0.673, 15.298)] and healthy controls was significant (p = 0.019). Significant difference of the miR-326 expression was found between new-onset SLE patients with serous cavity effusion and new-onset SLE patients without it(P<0.05). Significant positive correlation was found between the expression of miR-326 mRNA in Treg cells with CRP and anti-C1q antibody from new-onset SLE patients. 2. The level of Ets-1 mRNA was decreased in SLE patients [0.382(0.232, 0.572)] compared to healthy controls(p = 0.013). The difference was also found in new-onset SLE patients [0.222(0.125, 0.296)] while compared to healthy controls. Also, the level in new-onset SLE patients was lower than that in inactive SLE patients [0.482(0.398, 0.512)] (p = 0.001). 3. Negative correlation was found between miR-326 and Ets-1 mRNA expression in Treg cells from new-onset SLE patients (r = -0.583 p = 0.01). 4. There was no correlation of miR-326 or Ets-1 mRNA expression with SLEDAI. The increase of miR-326 expression in Treg cells from SLE patients may inhibit the expression of Ets-1 to participate in the pathological process of SLE.
    MeSH term(s) Adult ; Aged ; Humans ; Lupus Erythematosus, Systemic/genetics ; Lupus Erythematosus, Systemic/immunology ; MicroRNAs/genetics ; Middle Aged ; Proto-Oncogene Protein c-ets-1/genetics ; RNA, Messenger/genetics ; Real-Time Polymerase Chain Reaction ; T-Lymphocytes, Regulatory/immunology ; Young Adult
    Chemical Substances ETS1 protein, human ; MIRN326 microRNA, human ; MicroRNAs ; Proto-Oncogene Protein c-ets-1 ; RNA, Messenger
    Language English
    Publishing date 2016-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 434408-x
    ISSN 1573-2576 ; 0360-3997
    ISSN (online) 1573-2576
    ISSN 0360-3997
    DOI 10.1007/s10753-016-0312-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1401: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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