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  1. Article ; Online: L. reuteri

    Bai, Jin / Wang, Shuyang / Xu, Fuqiang / Dong, Miaoyin / Wang, Junkai / Sun, Xisi / Xiao, Guoqing

    International journal of radiation biology

    2023  Volume 99, Issue 5, Page(s) 779–790

    Abstract: Background: Probiotics such as : Methods: We investigated the therapeutic efficacy of : Results: Anti-proliferation effect of live probiotic combined with inactivated probiotic JMR-01 (LP + IP) on CT-26 reached a maximum of 39.55% among other ... ...

    Abstract Background: Probiotics such as
    Methods: We investigated the therapeutic efficacy of
    Results: Anti-proliferation effect of live probiotic combined with inactivated probiotic JMR-01 (LP + IP) on CT-26 reached a maximum of 39.55% among other experiment groups at 24 h when the ratio of cell to CFU was 1:1 in vitro. These activities have been fully validated in vivo, tumor-bearing mice treated by
    Conclusions: JMR-01 LP + IP adjuvant
    MeSH term(s) Animals ; Mice ; Limosilactobacillus reuteri ; Gastrointestinal Microbiome ; Lactobacillus ; Colonic Neoplasms/radiotherapy ; Carcinoma
    Language English
    Publishing date 2023-02-02
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3065-x
    ISSN 1362-3095 ; 0020-7616 ; 0955-3002
    ISSN (online) 1362-3095
    ISSN 0020-7616 ; 0955-3002
    DOI 10.1080/09553002.2023.2142979
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Integrative transcriptome and proteome analyses of Trichoderma longibrachiatum LC and its cellulase hyper-producing mutants generated by heavy ion mutagenesis reveal the key genes involved in cellulolytic enzymes regulation

    Dong, Miaoyin / Wang, Shuyang / Xu, Fuqiang / Xiao, Guoqing / Bai, Jin / Wang, Junkai / Sun, Xisi

    Biotechnology for biofuels and bioproducts. 2022 Dec., v. 15, no. 1

    2022  

    Abstract: BACKGROUND: The major challenge of facing the efficient utilization of biomass is the high cost of cellulolytic enzyme, while the Trichoderma longibrachiatum plays an essential role in the production of industrial enzymes and biomass recycling. RESULTS: ... ...

    Abstract BACKGROUND: The major challenge of facing the efficient utilization of biomass is the high cost of cellulolytic enzyme, while the Trichoderma longibrachiatum plays an essential role in the production of industrial enzymes and biomass recycling. RESULTS: The cellulase hyper‑producing mutants of LC-M4 and LC-M16 derived from the wild type T. longibrachiatum LC strain through heavy ion mutagenesis exhibited the high-efficiency secretion ability of cellulase and hemicellulose. The FPase activities of LC-M4 (4.51 IU/mL) and LC-M16 (4.16 IU/mL) mutants increased by 46.91% and 35.5% when compared to the LC strain, respectively. Moreover, these two cellulase hyper-producing mutants showed faster growth rate on the cellulosic substrates (Avicel and CMC-Na) plate than that of LC strain. Therefore, an integrative transcriptome and proteome profiling analysis of T. longibrachiatum LC and its cellulase hyper‑producing mutant LC-M4 and LC-M16 were employed to reveal the key genes involved in cellulolytic enzymes regulation. It was showed that the transcriptome and proteome profiles changed dramatically between the wild strain and mutant strains. Notably, the overlapped genes obtained from integrative analysis identified that the protein processing in ER involved in protein secretory pathway, starch and sucrose metabolism pathway and N-glycan biosynthesis pathway were significantly changed both in cellulase hyper-producing mutants and thereby improving the enzyme secretion efficiency, which maybe the main reason of cellulase hyper-production in LC-M4 and LC-M16 mutants. In addition, the three DEGs/DEPs (PDI, Sec61, VIP36) related with protein secretion in ER and two DEGs/DEPs (OST, MOGS) related with N-glycan biosynthesis were identified as key candidate genes participating in enzyme protein biosynthesis and secretion. CONCLUSIONS: In this study, a hypothetical secretory model of cellulase protein in filamentous fungi was established on the basis of DEGs/DEPs and key genes identified from the omics analysis, which were of great guidance on the rational genetic engineering and/or breeding of filamentous fungi for improving cellulase production.
    Keywords Trichoderma longibrachiatum ; biofuels ; biomass ; biosynthesis ; biotechnology ; endo-1,4-beta-glucanase ; hemicellulose ; models ; mutagenesis ; mutants ; protein secretion ; protein synthesis ; proteome ; starch ; sucrose ; transcriptome
    Language English
    Dates of publication 2022-12
    Size p. 63.
    Publishing place BioMed Central
    Document type Article
    ISSN 2731-3654
    DOI 10.1186/s13068-022-02161-7
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Integrative transcriptome and proteome analyses of Trichoderma longibrachiatum LC and its cellulase hyper-producing mutants generated by heavy ion mutagenesis reveal the key genes involved in cellulolytic enzymes regulation.

    Dong, Miaoyin / Wang, Shuyang / Xu, Fuqiang / Xiao, Guoqing / Bai, Jin / Wang, Junkai / Sun, Xisi

    Biotechnology for biofuels and bioproducts

    2022  Volume 15, Issue 1, Page(s) 63

    Abstract: Background: The major challenge of facing the efficient utilization of biomass is the high cost of cellulolytic enzyme, while the Trichoderma longibrachiatum plays an essential role in the production of industrial enzymes and biomass recycling.: ... ...

    Abstract Background: The major challenge of facing the efficient utilization of biomass is the high cost of cellulolytic enzyme, while the Trichoderma longibrachiatum plays an essential role in the production of industrial enzymes and biomass recycling.
    Results: The cellulase hyper‑producing mutants of LC-M4 and LC-M16 derived from the wild type T. longibrachiatum LC strain through heavy ion mutagenesis exhibited the high-efficiency secretion ability of cellulase and hemicellulose. The FPase activities of LC-M4 (4.51 IU/mL) and LC-M16 (4.16 IU/mL) mutants increased by 46.91% and 35.5% when compared to the LC strain, respectively. Moreover, these two cellulase hyper-producing mutants showed faster growth rate on the cellulosic substrates (Avicel and CMC-Na) plate than that of LC strain. Therefore, an integrative transcriptome and proteome profiling analysis of T. longibrachiatum LC and its cellulase hyper‑producing mutant LC-M4 and LC-M16 were employed to reveal the key genes involved in cellulolytic enzymes regulation. It was showed that the transcriptome and proteome profiles changed dramatically between the wild strain and mutant strains. Notably, the overlapped genes obtained from integrative analysis identified that the protein processing in ER involved in protein secretory pathway, starch and sucrose metabolism pathway and N-glycan biosynthesis pathway were significantly changed both in cellulase hyper-producing mutants and thereby improving the enzyme secretion efficiency, which maybe the main reason of cellulase hyper-production in LC-M4 and LC-M16 mutants. In addition, the three DEGs/DEPs (PDI, Sec61, VIP36) related with protein secretion in ER and two DEGs/DEPs (OST, MOGS) related with N-glycan biosynthesis were identified as key candidate genes participating in enzyme protein biosynthesis and secretion.
    Conclusions: In this study, a hypothetical secretory model of cellulase protein in filamentous fungi was established on the basis of DEGs/DEPs and key genes identified from the omics analysis, which were of great guidance on the rational genetic engineering and/or breeding of filamentous fungi for improving cellulase production.
    Language English
    Publishing date 2022-06-03
    Publishing country England
    Document type Journal Article
    ISSN 2731-3654
    ISSN (online) 2731-3654
    DOI 10.1186/s13068-022-02161-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The efficacy of prevention for colon cancer based on the microbiota therapy and the antitumor mechanisms with intervention of dietary

    Xu, Fuqiang / Li, Qiaoqiao / Wang, Shuyang / Dong, Miaoyin / Xiao, Guoqing / Bai, Jin / Wang, Junkai / Sun, Xisi

    Microbiology spectrum

    2023  , Page(s) e0018923

    Abstract: Gut microbiota and their secreted metabolites have an influence on the initiation and progression of colon cancer. Probiotics are extensively perceived as a potential microbiota-modulation strategy to promote the health of the host, while the ... ...

    Abstract Gut microbiota and their secreted metabolites have an influence on the initiation and progression of colon cancer. Probiotics are extensively perceived as a potential microbiota-modulation strategy to promote the health of the host, while the effectiveness of preventing colon cancer based on microbiota therapy has not been confirmed, and antitumor mechanisms influenced by microbiota and their metabolites with the intervention of probiotics remain to be further investigated.
    Language English
    Publishing date 2023-09-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.00189-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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