LIVIVO - Search results -

Search results

Result 1 - 10 of total 213

Search options

Article ; Online: LncRNA CADM1-AS1 serves as a new prognostic biomarker for gastric cancer.

Shi, X-Y / Sun, Y-Z / Li, M / Li, H-Y

European review for medical and pharmacological sciences

2019 Volume 23, Issue 3 Suppl, Page(s) 232–238

Abstract: Objective: To investigate the expression of the long non-coding RNA CADM1-AS1 (lncRNA CADM1-AS1) in gastric cancer and its clinical significance.: Patients and methods: The real time fluorescence quantitative polymerase chain reaction (qRT-PCR) was ... ...

Abstract	Objective: To investigate the expression of the long non-coding RNA CADM1-AS1 (lncRNA CADM1-AS1) in gastric cancer and its clinical significance. Patients and methods: The real time fluorescence quantitative polymerase chain reaction (qRT-PCR) was performed to detect the expression difference of lncRNA CADM1-AS1 between gastric cancer and its adjacent normal tissues. Then, the correlation between the expression of CADM1-AS1 in gastric cancer and the clinicopathological characteristics was analyzed by the Chi-square test. Moreover, the potential of lncRNA CADM1-AS1 in predicting the prognosis of patients with GC after the operation was assessed by the Log-rank test and the Cox's proportional hazards regression model. Results: The expression of lncRNA CADM1-AS1 was significantly decreased in tumor tissues. According to the mean expression of lncRNA CADM1-AS1, the patients were divided into a high expression group and a low expression group, and the expression of lncRNA CADM1-AS1 in gastric cancer was significantly correlated with tumor differentiation, N stage, M stage, and TNM stage. Moreover, the gastric cancer patients with higher expression of lncRNA CADM1-AS1 had a statistically better overall survival (OS) time and progression-free survival (PFS) time. In univariate analyses and multivariate analyses, the expression of lncRNA CADM1-AS1 was an independent prognosis index of patients with gastric cancer. Conclusions: LncRNA CADM1-AS1 might be a new prognostic biomarker for gastric cancer.
MeSH term(s)	Biomarkers, Tumor/genetics ; Down-Regulation ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; Neoplasm Staging ; Prognosis ; RNA, Long Noncoding/genetics ; Regression Analysis ; Stomach Neoplasms/genetics ; Stomach Neoplasms/mortality ; Stomach Neoplasms/pathology ; Survival Analysis
Chemical Substances	Biomarkers, Tumor ; RNA, Long Noncoding
Language	English
Publishing date	2019-08-05
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_201908_18652
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1887: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: LncDBH-AS1 knockdown enhances proliferation of non-small cell lung cancer cells by activating the Wnt signaling pathway via the miR-155/AXIN1 axis.

Shi, X-Y / Tao, X-F / Wang, G-W / He, J-F / Wu, L-F / Sun, Y-Z / Sun, X-J

European review for medical and pharmacological sciences

2021 Volume 25, Issue 1, Page(s) 139–144

Abstract: Objective: Dys-regulated long noncoding RNAs (lncRNAs) are involved in the cell growth of several malignancies and their aggressive phenotypes. LncRNA DBH-AS1 plays an important role in the advancement of various malignant tumors, but its contribution ... ...

Abstract	Objective: Dys-regulated long noncoding RNAs (lncRNAs) are involved in the cell growth of several malignancies and their aggressive phenotypes. LncRNA DBH-AS1 plays an important role in the advancement of various malignant tumors, but its contribution to non-small cell lung cancer (NSCLC) is still unexplored. This study intends to elucidate the role of the regulatory network of lncRNA DBH-AS1 in NSCLC progression. Patients and methods: The LncDBH-AS1 expression in 32 paired NSCLC patients' tissue samples and NSCLC cell lines were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The role of LncDBH-AS1 in NSCLC was investigated through cell counting kit-8 (CCK-8) assay and colony formation assay in vitro. Besides, the interaction between LncDBH-AS1 and miR-155 was also analyzed. Results: The DBH-AS1 expression was significantly down-regulated in NSCLC cell lines and tissue samples. Decreased DBH-AS1 levels promoted the in vitro proliferation of the NSCLC cells. The mechanism was that DBH-AS1 regulated AXIN1 expression by sponging miR-155 in NSCLC cell lines. Importantly, LncDBH-AS1 might inhibit WNT/β-CATENIN activation in NSCLC cells. Conclusions: The progression of NSCLC is facilitated by DBH-AS1 via miR-155 interaction and up-regulation of AXIN1 expression.
MeSH term(s)	Axin Protein/genetics ; Axin Protein/metabolism ; Carcinoma, Non-Small-Cell Lung/metabolism ; Carcinoma, Non-Small-Cell Lung/pathology ; Cell Proliferation ; Humans ; Lung Neoplasms/metabolism ; Lung Neoplasms/pathology ; MicroRNAs/metabolism ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; Tumor Cells, Cultured ; Wnt Signaling Pathway
Chemical Substances	AXIN1 protein, human ; Axin Protein ; MIRN155 microRNA, human ; MicroRNAs ; RNA, Long Noncoding
Language	English
Publishing date	2021-01-27
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_202101_24377
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1887: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: [Efficacy analysis of Altemeier and Delorme procedures for the rectal prolapse].

Xu, Y J / Zhang, D / Zhang, H / Sun, Y Z / Liu, C / Ren, D L

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery

2019 Volume 22, Issue 12, Page(s) 1170–1176

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Adolescent ; Adult ; Aged ; Colectomy/methods ; Colon, Sigmoid/surgery ; Female ; Humans ; Intestinal Mucosa/surgery ; Male ; Middle Aged ; Proctectomy/methods ; Quality of Life ; Rectal Prolapse/surgery ; Rectum/surgery ; Retrospective Studies ; Treatment Outcome ; Young Adult
Language	Chinese
Publishing date	2019-12-10
Publishing country	China
Document type	Journal Article
ISSN	1671-0274
ISSN	1671-0274
DOI	10.3760/cma.j.issn.1671-0274.2019.12.013
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: [Comparison on the clinical characteristics of patients with Takotsubo syndrome from China and from Europe/North America].

Ran, Q / Zhou, X / Sun, Y Z / Zhao, X / Liu, Z C / Liu, X / Qu, C / Zhang, C / Liang, J J / Yang, B / Shi, Shaobo

Zhonghua xin xue guan bing za zhi

2022 Volume 50, Issue 4, Page(s) 386–394

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Abdominal Pain/complications ; Adult ; Aged ; Angiotensin Receptor Antagonists ; Angiotensin-Converting Enzyme Inhibitors ; Anticoagulants ; Arrhythmias, Cardiac/complications ; China/epidemiology ; Diuretics ; Dizziness/complications ; Dyskinesias/complications ; Electrocardiography ; Europe/epidemiology ; Female ; Humans ; Hypotension/complications ; Middle Aged ; Platelet Aggregation Inhibitors ; Stroke Volume ; Takotsubo Cardiomyopathy/epidemiology ; Takotsubo Cardiomyopathy/etiology ; Ventricular Function, Left
Chemical Substances	Angiotensin Receptor Antagonists ; Angiotensin-Converting Enzyme Inhibitors ; Anticoagulants ; Diuretics ; Platelet Aggregation Inhibitors
Language	Chinese
Publishing date	2022-05-06
Publishing country	China
Document type	Journal Article
ZDB-ID	603425-1
ISSN	0253-3758
ISSN	0253-3758
DOI	10.3760/cma.j.cn112148-20220304-00152
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2276: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: DNAJA4 deficiency augments hyperthermia-induced Clusterin and ERK activation: two critical protective factors of human keratinocytes from hyperthermia-induced injury.

Wei, Z-D / Sun, Y-Z / Tu, C-X / Qi, R-Q / Huo, W / Chen, H-D / Gao, X-H

Journal of the European Academy of Dermatology and Venereology : JEADV

2020 Volume 34, Issue 10, Page(s) 2308–2317

Abstract: Background: Hyperthermia upregulates DNAJA4, a member of heat shock proteins (HSPs) 40 family, in human keratinocytes and HPV-infected tissue. DNAJA4 deficiency enhances growth arrest induced by hyperthermia. Clusterin (CLU) and phosphorylated ERK (p- ... ...

Abstract	Background: Hyperthermia upregulates DNAJA4, a member of heat shock proteins (HSPs) 40 family, in human keratinocytes and HPV-infected tissue. DNAJA4 deficiency enhances growth arrest induced by hyperthermia. Clusterin (CLU) and phosphorylated ERK (p-ERK) play a role in regulating cell proliferation and apoptosis, under environmental stress. Objectives: To examine the downstream molecules and signalling pathways of DNAJA4 and assess their roles in cell cycle and apoptosis of keratinocytes in response to hyperthermia. Methods: Wild-type and DNAJA4-knockout (KO) HaCaT cells were exposed to either 44 °C (hyperthermia) or 37 °C (control) for 30 min. The expression levels of CLU and p-ERK were determined by RT-PCR and Western blotting. RNAi and PD98059 were used to inhibit the expression of CLU and p-ERK, respectively. Cell viability, cell cycle and apoptosis were analysed by MTS assay and flow cytometry. Fresh biopsy samples of human normal foreskin or condyloma acuminatum (CA) were utilized to examine the expression of CLU and p-ERK after ex vivo culture at 44 °C. Results: The expression of CLU and p-ERK was significantly increased by hyperthermia treatment at 44 °C in HaCaT cells, foreskin and HPV-infected tissues. In HaCaT cells subjected to hyperthermia, DNAJA4 deficiency further augmented the expression of CLU and p-ERK. CLU deficiency enhanced the p-ERK expression. Hyperthermia-induced CLU and p-ERK exerted protective roles mainly through inhibiting apoptosis and maintaining cell cycle, respectively. Conclusions: In keratinocytes, CLU and p-ERK are induced by hyperthermia, an effect which can be further enhanced by DNAJA4 deficiency. CLU deficiency also increases p-ERK expression. Both CLU and p-ERK are critical protective factors of human keratinocytes from hyperthermia-induced injury.
MeSH term(s)	Apoptosis ; Clusterin/genetics ; HSP40 Heat-Shock Proteins ; Humans ; Hyperthermia ; Hyperthermia, Induced ; Keratinocytes ; Protective Factors
Chemical Substances	Clusterin ; DNAJA4 protein, human ; HSP40 Heat-Shock Proteins
Language	English
Publishing date	2020-05-15
Publishing country	England
Document type	Journal Article
ZDB-ID	1128828-0
ISSN	1468-3083 ; 0926-9959
ISSN (online)	1468-3083
ISSN	0926-9959
DOI	10.1111/jdv.16432
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 3492: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 413: Show issues

Order via subito

Details ▾

Article: [Compound matrine injection reduces morphine tolerance of the mice with lung cancer by inhibiting expression of multidrug resistance gene 1 and P-glycoprotein].

Sun, Y Z / You, R L / Wang, L / Ren, J S / Wang, D Y / Su, S J / Xu, R F

Zhonghua zhong liu za zhi [Chinese journal of oncology

2020 Volume 42, Issue 3, Page(s) 216–221

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	ATP Binding Cassette Transporter, Subfamily B, Member 1/genetics ; ATP Binding Cassette Transporter, Subfamily B, Member 1/metabolism ; Alkaloids/administration & dosage ; Alkaloids/adverse effects ; Animals ; Drug Resistance, Neoplasm/drug effects ; Genes, MDR ; Lung Neoplasms/genetics ; Lung Neoplasms/physiopathology ; Mice ; Morphine/pharmacology ; Quinolizines/administration & dosage ; Quinolizines/adverse effects
Chemical Substances	ATP Binding Cassette Transporter, Subfamily B, Member 1 ; Alkaloids ; Quinolizines ; Morphine (76I7G6D29C) ; matrine (N390W430AC)
Language	Chinese
Publishing date	2020-04-06
Publishing country	China
Document type	Journal Article
ZDB-ID	603424-x
ISSN	0253-3766
ISSN	0253-3766
DOI	10.3760/cma.j.cn112152-20190923-00620
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2277: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MO 321: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Investigations on oil detachment from rough surfaces in an aqueous solution

Xie, W. K / Sun, Y. Z / Liu, H. T / Zhang, F. H

RSC advances. 2017 June 15, v. 7, no. 49

2017

Abstract: Herein, detachment of oil molecules from perfect and defective aluminum oxide surfaces in an aqueous solution was investigated using atomistic molecular dynamics simulations. On the basis of the α-Al2O3 (0001) plane, three types of surface defects, pits, ...

Abstract	Herein, detachment of oil molecules from perfect and defective aluminum oxide surfaces in an aqueous solution was investigated using atomistic molecular dynamics simulations. On the basis of the α-Al2O3 (0001) plane, three types of surface defects, pits, grooves, and protrusions, were created and applied to explore the effects of surface defects on the detachment of oil molecules in an aqueous solution. Our simulation results indicate that the surface defects with various topographies affected the oil detachment to different degrees. The adsorption conformation of oil molecules on each defective alumina surface evidently changed during the entire simulation. The effect of each type of surface defect on oil detachment follows the order grooves < pits < protrusions. After this, the oil–alumina and water–alumina interactions were investigated to determine the effects of the surface defects on the driving forces responsible for the conformational change of oil molecules on each type of defective alumina surface. The microscopic conformational changes and detachment of oil molecules from the defective alumina surface discussed herein are believed to be helpful to enrich the mechanism of oil detachment from a rough substrate surface.
Keywords	adsorption ; aluminum oxide ; aqueous solutions ; molecular dynamics ; oils ; simulation models
Language	English
Dates of publication	2017-0615
Size	p. 31049-31060.
Publishing place	The Royal Society of Chemistry
Document type	Article
ISSN	2046-2069
DOI	10.1039/c7ra04766e
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: [Clinical significance of acute gastrointestinal injury grades in inflammatory response of critically ill patients].

Wang, J / Gao, Y L / Yu, W W / Xia, Y H / Sun, Y Z

Zhonghua yi xue za zhi

2017 Volume 97, Issue 42, Page(s) 3312–3315

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Acute Disease ; Calcitonin ; Critical Illness/classification ; Cytokines/metabolism ; Gastrointestinal Tract/immunology ; Gastrointestinal Tract/pathology ; Humans ; Inflammation ; Interleukin-6 ; Multiple Organ Failure/metabolism ; Systemic Inflammatory Response Syndrome ; Tumor Necrosis Factor-alpha
Chemical Substances	Cytokines ; Interleukin-6 ; Tumor Necrosis Factor-alpha ; Calcitonin (9007-12-9)
Language	Chinese
Publishing date	2017-11-14
Publishing country	China
Document type	Journal Article
ZDB-ID	132513-9
ISSN	0376-2491
ISSN	0376-2491
DOI	10.3760/cma.j.issn.0376-2491.2017.42.008
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2011: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Rumen chemical and bacterial changes during stepwise adaptation to a high-concentrate diet in goats.

Sun, Y Z / Mao, S Y / Zhu, W Y

Animal : an international journal of animal bioscience

2010 Volume 4, Issue 2, Page(s) 210–217

Abstract: The correlation between rumen chemical and bacterial changes was investigated during a four periodical stepwise adaptation to a high-concentrate diet (concentrate level at 0%, 30%, 50% and 70% for diet I to IV, respectively) in goats. The results showed ... ...

Abstract	The correlation between rumen chemical and bacterial changes was investigated during a four periodical stepwise adaptation to a high-concentrate diet (concentrate level at 0%, 30%, 50% and 70% for diet I to IV, respectively) in goats. The results showed that ruminal pH decreased from 6.7 to 5.5 after switching from diet I to II, and was maintained at about 5.5 on diet III. Denaturing gradient gel electrophoresis results showed that the rumen bacterial community was relatively stable during the initial three feeding periods, except for the appearance of three bands when diet changed from I to II, suggesting that an appropriate concentrate level can promote the proliferation of some bacteria. After 12 days of feeding diet III, total volatile fatty acid (VFA) concentration and butyrate proportion decreased. At days 2 and 3 of feeding diet IV, ruminal pH declined sharply to 5.3 and 4.7, respectively, and total VFA concentration decreased further while lactic acid concentration increased markedly, suggesting a relation between lactic acid accumulation and ruminal pH decline. At the same time, many bacteria disappeared, including most fibrolytic-related bacteria while Streptococcus bovis and Prevotella-like species dominated. Interestingly, Succinivibrio dextrinosolvens-like species maintained throughout the experiment, suggesting its tolerance to low pH. In conclusion, rumen bacterial community was relatively stable feeding 0% to 50% concentrate diets, and it was observed that appropriate concentrate levels in the diet could increase the diversity of rumen bacteria. However, concentrate-rich diets caused lactic acid accumulation and low ruminal pH that caused the disappearance of most fibrolytic-related bacteria sensitive to low pH while S. bovis and genus Prevotella persisted.
Language	English
Publishing date	2010-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2257920-5
ISSN	1751-732X ; 1751-7311
ISSN (online)	1751-732X
ISSN	1751-7311
DOI	10.1017/S175173110999111X
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 7718: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: An ultrasensitive SERS sensor for simultaneous detection of multiple cancer-related miRNAs.

Song, C Y / Yang, Y J / Yang, B Y / Sun, Y Z / Zhao, Y P / Wang, L H

Nanoscale

2016 Volume 8, Issue 39, Page(s) 17365–17373

Abstract: Simultaneous detection of multiple trace cancer associated serum miRNA biomarkers is considered as a feasible method for early cancer screening and diagnosis. In the present work, an ultrasensitive SERS sensor was prepared based on an Ag nanorod array ... ...

Abstract	Simultaneous detection of multiple trace cancer associated serum miRNA biomarkers is considered as a feasible method for early cancer screening and diagnosis. In the present work, an ultrasensitive SERS sensor was prepared based on an Ag nanorod array SERS substrate by assembling special hairpin-shaped molecular beacons (MBs) for the detection of multiple lung cancer-related miRNA biomarkers. The portable SERS sensor exhibits excellent performance for the qualitative and quantitative detection of miRNAs, with advantages of ultra-sensitivity, good specificity, uniformity, reproducibility and stability, as well as remarkable reusability. By monitoring the SERS signal quenching of the MBs in the presence of target miRNA biomarkers, three lung cancer related-miRNAs (miRNA-21, miRNA-486, and miRNA-375) in buffer and human serum were simultaneously assayed using the SERS sensor array, and the limits of detection of the three miRNAs in human serum are 393 aM, 176 aM, and 144 aM, respectively. The reliable results demonstrate that the proposed SERS sensor array can be a promising candidate with great potential for the screening and clinical diagnosis of cancer in the early stage.
MeSH term(s)	Biosensing Techniques ; Humans ; Lung Neoplasms/diagnosis ; Lung Neoplasms/genetics ; MicroRNAs/analysis ; MicroRNAs/blood ; Nanotubes ; Reproducibility of Results ; Spectrum Analysis, Raman
Chemical Substances	MicroRNAs
Language	English
Publishing date	2016-10-03
Publishing country	England
Document type	Journal Article
ZDB-ID	2515664-0
ISSN	2040-3372 ; 2040-3364
ISSN (online)	2040-3372
ISSN	2040-3364
DOI	10.1039/c6nr05504d
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito