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  1. Article ; Online: Method for highly selective, ultrasensitive fluorimetric detection of Cu

    Sharma, Shivani / Chayawan / Debnath, Joy / Sundar Ghosh, Kalyan

    Methods (San Diego, Calif.)

    2023  Volume 217, Page(s) 27–35

    Abstract: Schiff base probes (1 and 2) made from o-phenylenediamine and o-aminophenol were appeared as highly selective fluorimetric chemosensor of ... ...

    Abstract Schiff base probes (1 and 2) made from o-phenylenediamine and o-aminophenol were appeared as highly selective fluorimetric chemosensor of Cu
    MeSH term(s) Copper/chemistry ; Schiff Bases/chemistry ; Metals ; Ions ; Spectrometry, Fluorescence/methods ; Fluorescent Dyes/chemistry
    Chemical Substances Copper (789U1901C5) ; 1,2-diaminobenzene (8B713N8Q0F) ; 2-aminophenol (23RH73DZ65) ; Schiff Bases ; Metals ; Ions ; Fluorescent Dyes
    Language English
    Publishing date 2023-07-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1066584-5
    ISSN 1095-9130 ; 1046-2023
    ISSN (online) 1095-9130
    ISSN 1046-2023
    DOI 10.1016/j.ymeth.2023.06.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article ; Online: Enhancement in chaperone activity of human αA-crystallin by nanochaperone gold nanoparticles: Multispectroscopic studies on their molecular interactions.

    Sharma, Anchal / Rastogi, Harshita / Sundar Ghosh, Kalyan

    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

    2022  Volume 279, Page(s) 121344

    Abstract: The chaperone activity of human αA-crystallin (HAA) against aggregation of human γD-crystallin (HGD) was enhanced by gold nanoparticles (AuNPs). Chaperone activity of HAA was almost doubled in the presence of 5.5 nM gold nanoparticles (AuNPs). To ... ...

    Abstract The chaperone activity of human αA-crystallin (HAA) against aggregation of human γD-crystallin (HGD) was enhanced by gold nanoparticles (AuNPs). Chaperone activity of HAA was almost doubled in the presence of 5.5 nM gold nanoparticles (AuNPs). To decipher this effect at molecular level, interactions between HAA and AuNPs were studied using fluorescence and circular dichroism spectroscopic techniques. The nanoparticles were synthesized and characterized by using TEM and dynamic light scattering (DLS). TEM and DLS studies revealed that bioconjugation of AuNPs with HAA did not cause any significant change in the size of the nanoparticles. AuNPs had caused static quenching of tryptophan (Trp) fluorescence, which was confirmed through determination of excited state lifetime of Trp residue of HAA in absence and the presence of AuNPs. The association and quenching constant for HAA-AuNPs conjugation were ∼ 10
    MeSH term(s) Circular Dichroism ; Gold/chemistry ; Humans ; Metal Nanoparticles/chemistry ; Protein Folding ; Protein Structure, Secondary ; Spectrometry, Fluorescence ; Tryptophan
    Chemical Substances Gold (7440-57-5) ; Tryptophan (8DUH1N11BX)
    Language English
    Publishing date 2022-05-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 210413-1
    ISSN 1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
    ISSN (online) 1873-3557
    ISSN 0370-8322 ; 0584-8539 ; 1386-1425
    DOI 10.1016/j.saa.2022.121344
    Database MEDical Literature Analysis and Retrieval System OnLINE

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