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  1. Article ; Online: A

    Suo, Zehui / Cummings, Dale A / Puri, Aaron W / Schaefer, Amy L / Greenberg, E Peter

    mBio

    2023  Volume 14, Issue 4, Page(s) e0101023

    Abstract: Members of the ... ...

    Abstract Members of the genus
    MeSH term(s) Quorum Sensing/genetics ; Acyl-Butyrolactones/metabolism ; Mesorhizobium/genetics ; Mesorhizobium/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Trans-Activators/genetics ; Coenzyme A
    Chemical Substances Acyl-Butyrolactones ; Bacterial Proteins ; Trans-Activators ; Coenzyme A (SAA04E81UX)
    Language English
    Publishing date 2023-05-25
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.01010-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The Wsp system of

    O'Neal, Lindsey / Baraquet, Claudine / Suo, Zehui / Dreifus, Julia E / Peng, Yun / Raivio, Tracy L / Wozniak, Daniel J / Harwood, Caroline S / Parsek, Matthew R

    Proceedings of the National Academy of Sciences of the United States of America

    2022  Volume 119, Issue 18, Page(s) e2117633119

    Abstract: Surface sensing is a critical process that promotes the transition to a biofilm lifestyle. Several surface-sensing mechanisms have been described for a range of species, most involving surface appendages, such as flagella and pili. Pseudomonas aeruginosa ...

    Abstract Surface sensing is a critical process that promotes the transition to a biofilm lifestyle. Several surface-sensing mechanisms have been described for a range of species, most involving surface appendages, such as flagella and pili. Pseudomonas aeruginosa uses the Wsp chemosensory-like signal transduction pathway to sense surfaces and promote biofilm formation. The methyl-accepting chemotaxis protein WspA recognizes an unknown surface-associated signal and initiates a phosphorylation cascade that activates the diguanylate cyclase WspR. We conducted a screen for Wsp-activating compounds and found that chemicals that impact the cell envelope induce Wsp signaling, increase intracellular c-di-GMP levels, and can promote surface attachment. To isolate the Wsp system from other P. aeruginosa surface-sensing systems, we heterologously expressed it in Escherichia coli and found it sufficient for sensing surfaces and the chemicals identified in our screen. Using well-characterized reporters for different E. coli cell envelope stress responses, we then determined that Wsp sensitivity overlapped with multiple E. coli cell envelope stress-response systems. Using mutational and CRISPRi analysis, we found that misfolded proteins in the periplasm appear to be a major stimulus of the Wsp system. Finally, we show that surface attachment appears to have an immediate, observable effect on cell envelope integrity. Collectively, our results provide experimental evidence that cell envelope stress represents an important feature of surface sensing in P. aeruginosa.
    MeSH term(s) Biofilms ; Cell Membrane/metabolism ; Cell Wall ; Periplasm ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/metabolism
    Language English
    Publishing date 2022-04-27
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2117633119
    Database MEDical Literature Analysis and Retrieval System OnLINE

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