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  1. Article ; Online: Type 1-type 2 interferon imbalance in dry eye disease.

    Panigrahi, Trailokyanath / D'Souza, Sharon / Suresh Babu, Vishnu / Dickman, Mor M / Nuijts, Rudy M M A / Sethu, Swaminathan / Shetty, Rohit

    Indian journal of ophthalmology

    2023  Volume 71, Issue 4, Page(s) 1526–1532

    Abstract: Purpose: Dry eye disease (DED) is characterized by altered ocular surface proinflammatory and antiinflammatory factors. Interferons (IFNs) are a class of pleiotropic cytokines well known for their antimicrobial, inflammatory, and immunomodulatory roles. ...

    Abstract Purpose: Dry eye disease (DED) is characterized by altered ocular surface proinflammatory and antiinflammatory factors. Interferons (IFNs) are a class of pleiotropic cytokines well known for their antimicrobial, inflammatory, and immunomodulatory roles. Hence, this study investigates the ocular surface expression of different types of IFNs in patients with DED.
    Methods: The cross-sectional, observational study included patients with DED and normal subjects. Conjunctival impression cytology (CIC) samples were obtained from the study subjects (controls, n = 7; DED, n = 8). The mRNA expression levels of type 1 IFN (IFNα, IFNβ), type 2 IFN (IFNγ), and type 3 IFN (IFNλ1, IFNλ2, IFNλ3) were measured by quantitative PCR (polymerase chain reaction) in CIC samples. IFNα and IFNγ expression under hyperosmotic stress was also studied in human corneal epithelial cells (HCECs) in vitro.
    Results: The mRNA expression levels of IFNα and IFNβ were significantly lower and that of IFNγ was significantly higher in DED patients compared to healthy controls. The mRNA levels of IFNα, IFNβ, and IFNλ were significantly lower compared to IFNγ in DED patients. An inverse association between tonicity-responsive enhancer-binding protein (TonEBP; hyperosmotic stress maker) and IFNα or IFNβ expression and a positive association between TonEBP and IFNγ expression was observed in CIC samples. The expression of IFNα was lower than IFNγ in HCECs undergoing hyperosmotic stress compared to HCECs without the stress.
    Conclusion: The presence of an imbalance between type 1 and type 2 IFNs in DED patients suggests newer pathogenic processes in DED, plausible ocular surface infection susceptibility in DED patients, and potential therapeutic targets in the management of DED.
    MeSH term(s) Humans ; Cross-Sectional Studies ; Cytokines/metabolism ; Interferon-alpha ; Dry Eye Syndromes/diagnosis ; Dry Eye Syndromes/genetics ; Dry Eye Syndromes/metabolism ; Epithelial Cells/metabolism
    Chemical Substances Cytokines ; Interferon-alpha
    Language English
    Publishing date 2023-03-09
    Publishing country India
    Document type Observational Study ; Journal Article
    ZDB-ID 187392-1
    ISSN 1998-3689 ; 0301-4738
    ISSN (online) 1998-3689
    ISSN 0301-4738
    DOI 10.4103/IJO.IJO_2842_22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 598: Show issues

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  2. Article ; Online: Lack of Retinoblastoma Protein Shifts Tumor Metabolism from Glycolysis to OXPHOS and Allows the Use of Alternate Fuels.

    Suresh Babu, Vishnu / Dudeja, Gagan / Sa, Deepak / Bisht, Anadi / Shetty, Rohit / Heymans, Stephane / Guha, Nilanjan / Ghosh, Arkasubhra

    Cells

    2022  Volume 11, Issue 20

    Abstract: Mutations in ... ...

    Abstract Mutations in the
    MeSH term(s) Child ; Humans ; Retinoblastoma Protein/genetics ; NAD/metabolism ; Hexokinase/metabolism ; Glutamine/metabolism ; Retinoblastoma ; Glycolysis/genetics ; Glucose/metabolism ; Fatty Acids/metabolism ; Retinal Neoplasms ; Adenosine Triphosphate/metabolism ; Transcription Factors/metabolism ; Carnitine ; Tricarboxylic Acids ; Pyruvates
    Chemical Substances Retinoblastoma Protein ; NAD (0U46U6E8UK) ; Hexokinase (EC 2.7.1.1) ; Glutamine (0RH81L854J) ; Glucose (IY9XDZ35W2) ; Fatty Acids ; Adenosine Triphosphate (8L70Q75FXE) ; Transcription Factors ; Carnitine (S7UI8SM58A) ; Tricarboxylic Acids ; Pyruvates
    Language English
    Publishing date 2022-10-11
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11203182
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Selective Induction of Intrinsic Apoptosis in Retinoblastoma Cells by Novel Cationic Antimicrobial Dodecapeptides.

    Suresh Babu, Vishnu / Kizhakeyil, Atish / Dudeja, Gagan / Chaurasia, Shyam S / Barathi, Veluchami Amutha / Heymans, Stephane / Verma, Navin Kumar / Lakshminarayanan, Rajamani / Ghosh, Arkasubhra

    Pharmaceutics

    2022  Volume 14, Issue 11

    Abstract: Host defense peptides represent an important component of innate immunity. In this work, we report the anticancer properties of a panel of hyper-charged wholly cationic antimicrobial dodecapeptides (CAPs) containing multiple canonical forms of lysine and ...

    Abstract Host defense peptides represent an important component of innate immunity. In this work, we report the anticancer properties of a panel of hyper-charged wholly cationic antimicrobial dodecapeptides (CAPs) containing multiple canonical forms of lysine and arginine residues. These CAPs displayed excellent bactericidal activities against a broad range of pathogenic bacteria by dissipating the cytoplasmic membrane potential. Specifically, we identified two CAPs, named HC3 and HC5, that effectively killed a significant number of retinoblastoma (WERI-Rb1) cells (
    Language English
    Publishing date 2022-11-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527217-2
    ISSN 1999-4923
    ISSN 1999-4923
    DOI 10.3390/pharmaceutics14112507
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  4. Article: Enhanced Epithelial-to-Mesenchymal Transition and Chemoresistance in Advanced Retinoblastoma Tumors Is Driven by miR-181a.

    Suresh Babu, Vishnu / Bisht, Anadi / Mallipatna, Ashwin / Sa, Deepak / Dudeja, Gagan / Kannan, Ramaraj / Shetty, Rohit / Guha, Nilanjan / Heymans, Stephane / Ghosh, Arkasubhra

    Cancers

    2022  Volume 14, Issue 20

    Abstract: Advanced retinoblastoma (Rb) tumors display high metastatic spread to distant tissues, causing a potent threat to vision and life. Through transcriptomic profiling, we discovered key upregulated genes that belonged to the epithelial-mesenchymal ... ...

    Abstract Advanced retinoblastoma (Rb) tumors display high metastatic spread to distant tissues, causing a potent threat to vision and life. Through transcriptomic profiling, we discovered key upregulated genes that belonged to the epithelial-mesenchymal transition (EMT) and chemotherapy resistance pathways in advanced Rb tumors. Through in vitro models, we further showed that Rb null tumor cells under prolonged chemo drug exposure, acquires a metastasis-like phenotype through the EMT program mediated by ZEB1 and SNAI2 and these cells further acquires chemotherapeutic resistance through cathepsin-L- and MDR1-mediated drug efflux mechanisms. Using a miRNA microarray, we identified miR-181a-5p as being significantly reduced in advanced Rb tumors, which was associated with an altered EMT and drug-resistance genes. We showed that enhancing miR-181a-5p levels in Rb null chemo-resistant sublines reduced the ZEB1 and SNAI2 levels and halted the mesenchymal transition switch, further reducing the drug resistance. We thus identified miR-181a-5p as a therapeutically exploitable target for EMT-triggered drug-resistant cancers that halted their invasion and migration and sensitized them to low-dose chemotherapy drugs.
    Language English
    Publishing date 2022-10-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers14205124
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Retinoblastoma genetics screening and clinical management.

    Gupta, Himika / Malaichamy, Sivasankar / Mallipatna, Ashwin / Murugan, Sakthivel / Jeyabalan, Nallathambi / Suresh Babu, Vishnu / Ghosh, Anuprita / Ghosh, Arkasubhra / Santhosh, Sam / Seshagiri, Somasekar / Ramprasad, Vedam L / Kumaramanickavel, Govindasamy

    BMC medical genomics

    2021  Volume 14, Issue 1, Page(s) 188

    Abstract: Background: India accounts for 20% of the global retinoblastoma (RB) burden. However, the existing data on RB1 gene germline mutations and its influence on clinical decisions is minimally explored.: Methods: Fifty children with RB underwent complete ... ...

    Abstract Background: India accounts for 20% of the global retinoblastoma (RB) burden. However, the existing data on RB1 gene germline mutations and its influence on clinical decisions is minimally explored.
    Methods: Fifty children with RB underwent complete clinical examination and appropriate multidisciplinary management. Screening of germline RB1 gene mutations was performed through next-generation sequencing and Multiplex Ligation-dependent Probe Amplification (MLPA) analysis. The mutation and non-mutation groups were compared for clinical parameters especially severity, progression and recurrence.
    Results: Twenty-nine patients had bilateral RB (BLRB) and 21 had unilateral RB (ULRB). The genetic analysis revealed 20 RB1 variations in 29 probands, inclusive of 3 novel mutations, known 16 mutations and heterozygous whole gene deletions. The mutation detection rate (MDR) was 86.2% in BLRB and 19% in ULRB. Associations of disease recurrence (p = 0.021), progression (p = 0.000) and higher percentage of optic nerve invasion, subretinal seeds and high-risk pathological factors were observed in the mutation group. Clinical management was influenced by the presence of germline mutations, particularly while deciding on enucleation, frequency of periodic follow up and radiotherapy.
    Conclusions: We identified novel RB1 mutations, and our mutation detection rate was on par with the previous global studies. In our study, genetic results influenced clinical management and we suggest that it should be an essential and integral component of RB-care in India and elsewhere.
    MeSH term(s) Retinoblastoma
    Language English
    Publishing date 2021-07-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1755-8794
    ISSN (online) 1755-8794
    DOI 10.1186/s12920-021-01034-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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