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  1. Article ; Online: Elevated Expression of ADAM10 in Skeletal Muscle of Patients with Idiopathic Inflammatory Myopathies Could Be Responsible for FNDC5/Irisin Unbalance.

    Zerlotin, Roberta / Fornaro, Marco / Errede, Mariella / Pignataro, Patrizia / Suriano, Clelia / Ruggieri, Maddalena / Colucci, Silvia / Iannone, Florenzo / Grano, Maria / Colaianni, Graziana

    International journal of molecular sciences

    2023  Volume 24, Issue 3

    Abstract: Dermatomyositis (DM) and immune-mediated necrotizing myopathy (IMNM) are two rare diseases belonging to the group of idiopathic inflammatory myopathies (IIM). Muscle involvement in DM is characterized by perifascicular atrophy and poor myofiber necrosis, ...

    Abstract Dermatomyositis (DM) and immune-mediated necrotizing myopathy (IMNM) are two rare diseases belonging to the group of idiopathic inflammatory myopathies (IIM). Muscle involvement in DM is characterized by perifascicular atrophy and poor myofiber necrosis, while IMNM is characterized by myofiber necrosis with scarce inflammatory infiltrates. Muscle biopsies and laboratory tests are helpful in diagnosis, but currently, few biomarkers of disease activity and progression are available. In this context, we conducted a cohort study of forty-one DM and IMNM patients, aged 40-70 years. In comparison with control subjects, in the muscle biopsies of these patients, there was a lower expression of FNDC5, the precursor of irisin, a myokine playing a key role in musculoskeletal metabolism. Expectedly, the muscle cross-sectional areas of these patients were reduced, while, surprisingly, serum irisin levels were higher than in CTRL, as were mRNA levels of ADAM10, a metalloproteinase recently shown to be the cleavage agent for FNDC5. We hypothesize that elevated expression of ADAM10 in the skeletal muscle of DM and IMNM patients might be responsible for the discrepancy between irisin levels and FNDC5 expression. Future studies will be needed to understand the mechanisms underlying exacerbated FNDC5 cleavage and muscle irisin resistance in these inflammatory myopathies.
    MeSH term(s) Humans ; Fibronectins/metabolism ; Cohort Studies ; Muscle, Skeletal/metabolism ; Myositis/metabolism ; Transcription Factors/metabolism ; Autoimmune Diseases/metabolism ; Necrosis/metabolism ; ADAM10 Protein/genetics ; ADAM10 Protein/metabolism ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Amyloid Precursor Protein Secretases/metabolism
    Chemical Substances Fibronectins ; Transcription Factors ; ADAM10 protein, human (EC 3.4.24.81) ; ADAM10 Protein (EC 3.4.24.81) ; Membrane Proteins ; Amyloid Precursor Protein Secretases (EC 3.4.-) ; FNDC5 protein, human
    Language English
    Publishing date 2023-01-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24032469
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: RTG Signaling Sustains Mitochondrial Respiratory Capacity in HOG1-Dependent Osmoadaptation

    Guaragnella, Nicoletta / Agrimi, Gennaro / Scarcia, Pasquale / Suriano, Clelia / Pisano, Isabella / Bobba, Antonella / Mazzoni, Cristina / Palmieri, Luigi / Giannattasio, Sergio

    Microorganisms. 2021 Sept. 06, v. 9, no. 9

    2021  

    Abstract: Mitochondrial RTG-dependent retrograde signaling, whose regulators have been characterized in Saccharomyces cerevisiae, plays a recognized role under various environmental stresses. Of special significance, the activity of the transcriptional complex ... ...

    Abstract Mitochondrial RTG-dependent retrograde signaling, whose regulators have been characterized in Saccharomyces cerevisiae, plays a recognized role under various environmental stresses. Of special significance, the activity of the transcriptional complex Rtg1/3 has been shown to be modulated by Hog1, the master regulator of the high osmolarity glycerol pathway, in response to osmotic stress. The present work focuses on the role of RTG signaling in salt-induced osmotic stress and its interaction with HOG1. Wild-type and mutant cells, lacking HOG1 and/or RTG genes, are compared with respect to cell growth features, retrograde signaling activation and mitochondrial function in the presence and in the absence of high osmostress. We show that RTG2, the main upstream regulator of the RTG pathway, contributes to osmoadaptation in an HOG1-dependent manner and that, with RTG3, it is notably involved in a late phase of growth. Our data demonstrate that impairment of RTG signaling causes a decrease in mitochondrial respiratory capacity exclusively under osmostress. Overall, these results suggest that HOG1 and the RTG pathway may interact sequentially in the stress signaling cascade and that the RTG pathway may play a role in inter-organellar metabolic communication for osmoadaptation.
    Keywords Saccharomyces cerevisiae ; cell growth ; glycerol ; mitochondria ; mutants ; osmolarity ; osmotic stress ; osmotolerance ; transcription (genetics)
    Language English
    Dates of publication 2021-0906
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9091894
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Short-Term Irisin Treatment Enhanced Neurotrophin Expression Differently in the Hippocampus and the Prefrontal Cortex of Young Mice.

    Dicarlo, Manuela / Pignataro, Patrizia / Zerlotin, Roberta / Suriano, Clelia / Zecca, Chiara / Dell'Abate, Maria Teresa / Storlino, Giuseppina / Oranger, Angela / Sanesi, Lorenzo / Mori, Giorgio / Grano, Maria / Colaianni, Graziana / Colucci, Silvia

    International journal of molecular sciences

    2023  Volume 24, Issue 11

    Abstract: As a result of physical exercise, muscle releases multiple exerkines, such as "irisin", which is thought to induce pro-cognitive and antidepressant effects. We recently demonstrated in young healthy mice the mitigation of depressive behaviors induced by ... ...

    Abstract As a result of physical exercise, muscle releases multiple exerkines, such as "irisin", which is thought to induce pro-cognitive and antidepressant effects. We recently demonstrated in young healthy mice the mitigation of depressive behaviors induced by consecutive 5 day irisin administration. To understand which molecular mechanisms might be involved in such effect, we here studied, in a group of mice previously submitted to a behavioral test of depression, the gene expression of neurotrophins and cytokines in the hippocampus and prefrontal cortex (PFC), two brain areas frequently investigated in the depression pathogenesis. We found significantly increased mRNA levels of nerve growth factor (NGF) and fibroblast growth factor 2 (FGF-2) in the hippocampus and brain-derived growth factor (BDNF) in the PFC. We did not detect a difference in the mRNA levels of interleukin 6 (IL-6) and IL-1β in both brain regions. Except for BDNF in the PFC, two-way ANOVA analysis did not reveal sex differences in the expression of the tested genes. Overall, our data evidenced a site-specific cerebral modulation of neurotrophins induced by irisin treatment in the hippocampus and the PFC, contributing to the search for new antidepressant treatments targeted at single depressive events with short-term protocols.
    MeSH term(s) Mice ; Female ; Male ; Animals ; Brain-Derived Neurotrophic Factor/metabolism ; Antidepressive Agents/pharmacology ; Hippocampus/metabolism ; Prefrontal Cortex/metabolism ; RNA, Messenger/metabolism
    Chemical Substances Brain-Derived Neurotrophic Factor ; Antidepressive Agents ; RNA, Messenger
    Language English
    Publishing date 2023-05-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24119111
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Once-Daily Subcutaneous Irisin Administration Mitigates Depression- and Anxiety-like Behavior in Young Mice.

    Pignataro, Patrizia / Dicarlo, Manuela / Suriano, Clelia / Sanesi, Lorenzo / Zerlotin, Roberta / Storlino, Giuseppina / Oranger, Angela / Zecca, Chiara / Dell'Abate, Maria Teresa / Mori, Giorgio / Grano, Maria / Colucci, Silvia / Colaianni, Graziana

    International journal of molecular sciences

    2023  Volume 24, Issue 7

    Abstract: Major depression is one of the most common psychiatric disorders worldwide, usually associated with anxiety. The multi-etiological nature of depression has increased the search for new antidepressant molecules, including irisin, for which, in a previous ... ...

    Abstract Major depression is one of the most common psychiatric disorders worldwide, usually associated with anxiety. The multi-etiological nature of depression has increased the search for new antidepressant molecules, including irisin, for which, in a previous study, we tested its effect in young mice when administered intraperitoneally in a long-term intermittent manner. Here, we evaluated the effect of subcutaneous short-term irisin administration (100 µg/Kg/day/5 days) in male and female mice subjected to behavioral paradigms: Tail Suspension Test (TST), Forced Swim Test (FST), Elevated Plus Maze (EPM), and Y Maze (YM). Moreover, a qRT-PCR assay was performed to analyze the impact of irisin treatment on Pgc-1α/FNDC5 expression in the brain. A significant reduction in immobility time in TST and FST was observed in irisin-treated mice. Furthermore, irisin treatment significantly increased the number of entries and time spent in open arms, demonstrating its anxiolytic effect. Memory-enhancing effects were not reported in YM. Interestingly, no gender differences were observed in all behavioral tests. Overall, these results suggest that short-term subcutaneous irisin administration can exert an antidepressant and anxiolytic role, probably due to the activation of the Pgc-1α/FNDC5 system in the brain. Further investigation could lead to the identification of irisin as a new agent for the treatment of psychiatric disorders.
    MeSH term(s) Mice ; Male ; Female ; Animals ; Depression/drug therapy ; Depression/metabolism ; Fibronectins/metabolism ; Anxiety/drug therapy ; Antidepressive Agents/pharmacology ; Anti-Anxiety Agents/pharmacology ; Behavior, Animal
    Chemical Substances Fibronectins ; Antidepressive Agents ; Anti-Anxiety Agents ; FNDC5 protein, mouse
    Language English
    Publishing date 2023-04-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24076715
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Irisin Protects against Loss of Trabecular Bone Mass and Strength in Adult Ovariectomized Mice by Stimulating Osteoblast Activity.

    Storlino, Giuseppina / Dicarlo, Manuela / Zerlotin, Roberta / Pignataro, Patrizia / Sanesi, Lorenzo / Suriano, Clelia / Oranger, Angela / Mori, Giorgio / Passeri, Giovanni / Colucci, Silvia / Grano, Maria / Colaianni, Graziana

    International journal of molecular sciences

    2023  Volume 24, Issue 12

    Abstract: Irisin is a peptide secreted by skeletal muscle that plays a major role in bone metabolism. Experiments in mouse models have shown that administration of recombinant irisin prevents disuse-induced bone loss. In this study, we aimed to evaluate the ... ...

    Abstract Irisin is a peptide secreted by skeletal muscle that plays a major role in bone metabolism. Experiments in mouse models have shown that administration of recombinant irisin prevents disuse-induced bone loss. In this study, we aimed to evaluate the effects of irisin treatment for the prevention of bone loss in the ovariectomized (Ovx) mouse, the animal model commonly used to investigate osteoporosis caused by estrogen deficiency. Micro-Ct analysis conducted on Sham mice (Sham-veh) and Ovx mice treated with vehicle (Ovx-veh) or recombinant irisin (Ovx-irisn) showed bone volume fraction (BV/TV) decreases in femurs (Ovx-veh 1.39± 0.71 vs. Sham-veh 2.84 ± 1.23;
    MeSH term(s) Mice ; Animals ; Female ; Humans ; Fibronectins/pharmacology ; Cancellous Bone/pathology ; Osteoporosis/pathology ; Bone Diseases, Metabolic ; Disease Models, Animal ; Osteoblasts/pathology ; Ovariectomy/adverse effects ; Bone Density
    Chemical Substances Fibronectins
    Language English
    Publishing date 2023-06-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24129896
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Time-dependent unloading effects on muscle and bone and involvement of FNDC5/irisin axis.

    Sanesi, Lorenzo / Storlino, Giuseppina / Dicarlo, Manuela / Oranger, Angela / Zerlotin, Roberta / Pignataro, Patrizia / Suriano, Clelia / Guida, Gabriella / Grano, Maria / Colaianni, Graziana / Colucci, Silvia Concetta

    NPJ microgravity

    2023  Volume 9, Issue 1, Page(s) 4

    Abstract: The identification of biomarkers and countermeasures to prevent the adverse effects on the musculoskeletal system caused by the absence of mechanical loading is the main goal of space biomedical research studies. In this study, we analyzed over 4 weeks ... ...

    Abstract The identification of biomarkers and countermeasures to prevent the adverse effects on the musculoskeletal system caused by the absence of mechanical loading is the main goal of space biomedical research studies. In this study, we analyzed over 4 weeks of unloading, the modulation in the expression of key proteins in Vastus lateralis, Gastrocnemius and cortical bone in parallel with the modulation of irisin serum levels and its precursor FNDC5 in skeletal muscle of hind limb unloaded (HU) mice. Here we report that Atrogin-1 was up-regulated as early as 1- and 2-week of unloading, whereas Murf-1 at 2- and 3-weeks, along with a marked modulation in the expression of myosin heavy chain isoforms during unloading. Since HU mice showed reduced irisin serum levels at 4-weeks, as well as FNDC5 decrease at 3- and 4-weeks, we treated HU mice with recombinant irisin for 4 weeks, showing that unloading-dependent decline of myosin heavy chain isoforms, MyHCIIα and MyHCIIx, and the anti-apoptotic factor Bcl2, were prevented. In parallel, irisin treatment inhibited the increase of the senescence marker p53, and the pro-apoptotic factor Bax. Overall, these results suggest that the myokine irisin could be a possible therapy to counteract the musculoskeletal impairment caused by unloading.
    Language English
    Publishing date 2023-01-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2823626-9
    ISSN 2373-8065
    ISSN 2373-8065
    DOI 10.1038/s41526-023-00251-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: RTG

    Guaragnella, Nicoletta / Agrimi, Gennaro / Scarcia, Pasquale / Suriano, Clelia / Pisano, Isabella / Bobba, Antonella / Mazzoni, Cristina / Palmieri, Luigi / Giannattasio, Sergio

    Microorganisms

    2021  Volume 9, Issue 9

    Abstract: ... ...

    Abstract Mitochondrial
    Language English
    Publishing date 2021-09-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9091894
    Database MEDical Literature Analysis and Retrieval System OnLINE

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