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Article ; Online: GST-Perfringolysin O production for the localization and quantification of membrane cholesterol in human and mouse brain and liver

Leire Goicoechea / Fabian Arenas / Fernanda Castro / Susana Nuñez / Sandra Torres / Carmen Garcia-Ruiz / José C. Fernandez-Checa

STAR Protocols, Vol 3, Iss 1, Pp 101068- (2022)

2022

Abstract: Summary: Abnormal cholesterol metabolism is linked to many neurodegenerative disorders. Here, we present a protocol for the production of a recombinant protein consisting of a Glutathione-S-Transferase tag fused with the Perfringolysin O (PFO). The GST- ... ...

Abstract	Summary: Abnormal cholesterol metabolism is linked to many neurodegenerative disorders. Here, we present a protocol for the production of a recombinant protein consisting of a Glutathione-S-Transferase tag fused with the Perfringolysin O (PFO). The GST-PFO tag enables analysis of the localization of cholesterol in subcellular membranes of human and mice brain and liver tissues. We have used this approach for samples from Niemann-Pick type C disease and non-alcoholic steatohepatitis models. The construct may also have applications for the diagnosis of cholesterol-accumulating disorders.For complete details on the use and execution of this protocol, please refer to Kwiatkowska et al. (2014).
Keywords	Cell Biology ; Cell Membrane ; Health Sciences ; Metabolism ; Microscopy ; Model Organisms ; Science (General) ; Q1-390
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Dr. Enrique Suárez Hernández, eminent professor of the Institute of Basic and Pre-Clinical Sciences “Victoria de Girón”

Gabriela Alonso Bermúdez / Susana Núñez Martineaux / Bárbara Ivette Palmeiro Vivanco

Revista Habanera de Ciencias Médicas, Vol 18, Iss 5, Pp 697-

2019 Volume 703

Abstract: Dr. Enrique Alberto Suárez Hernández enrolled in the medical studies at the University of Havana in 1938 and graduated in 1945. He worked as Municipal Doctor in the East of Cuba, forensic doctor in the region of Banes, doctor in Guanabacoa, and Professor ...

Abstract	Dr. Enrique Alberto Suárez Hernández enrolled in the medical studies at the University of Havana in 1938 and graduated in 1945. He worked as Municipal Doctor in the East of Cuba, forensic doctor in the region of Banes, doctor in Guanabacoa, and Professor of Anatomy in the Medical School of the University of Havana. During the years of the clandestineness, he collaborated with combatants and farmers that went to see him with health problems. After the triumph of the Cuban Revolution, he became member of the National Revolutionary Militia. Dr. Enrique was one of the founders of the “Comités de Defensa de la Revolución” (CDR) and a member of the “Central de Trabajadores de Cuba” (CTC). He participated in mobilizations and voluntary works. Dr. Enrique Suárez Hernández was one of founders of the Institute of Basic and Pre-Clinical Sciences “Victoria de Girón”; full professor of Anatomy.
Keywords	médico ; enrique suárez hernández ; profesor ; egregio ; fundador ; instituto de ciencias básicas y preclínicas victoria de girón ; Medicine (General) ; R5-920 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2019-11-01T00:00:00Z
Publisher	Universidad de Ciencias Médicas de La Habana
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Dr. Eduardo Sabas Alomá, professor of the cuban school of medicine

Gabriela Alonso Bermúdez / Susana Núñez Martineaux / Sobeida Dreke Freire

Revista Habanera de Ciencias Médicas, Vol 18, Iss 6, Pp 855-

2019 Volume 862

Abstract: Dr. Eduardo Sabas Alomá studied Medicine in the University of Havana and carried out postgraduate studies at Harvard University, the United States of America and at the University of San Marcos in Peru. He participated in revolutionary and political ... ...

Abstract	Dr. Eduardo Sabas Alomá studied Medicine in the University of Havana and carried out postgraduate studies at Harvard University, the United States of America and at the University of San Marcos in Peru. He participated in revolutionary and political activities against the government ruled by the dictator Gerardo Machado that led him to the exile in the United States. He returned to Cuba in 1937. After Batista´s coup d´état in 1952, he was interrupted because of his activities against the regime. He was professor of Physiology in the Medical Faculty of the University of Havana and professor of Bacteriology in the Nursing School located in “Calixto García” University Hospital; he also worked as a doctor in this hospital and in “Maternidad Obrera” Hospital. After the triumph of the Revolution, he was member of the Medical School teaching staff while most of the doctors renounced their professional work and went to the United States of America encouraged by the counterrevolution. He was founder of the Higher Institute of Basic and Pre-Clinical Sciences “Victoria de Girón” and Full Professor of Physiology.
Keywords	médico ; profesor ; estudios de postgrado ; actividades revolucionarias ; escuela cubana de medicina ; fundador ; Medicine (General) ; R5-920 ; Public aspects of medicine ; RA1-1270
Subject code	900
Language	English
Publishing date	2019-10-01T00:00:00Z
Publisher	Universidad de Ciencias Médicas de La Habana
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Experiences in the training of specialist family and community nurses

Francisca Sánchez-Muñoz / Isabel María Fernández-Medina / José Granero-Molina / Iván Claudio Suazo-Galdames / Susana Nunez-Nagy / María Isabel Ventura-Miranda / María Dolores Ruíz-Fernández

Frontiers in Public Health, Vol

a qualitative study

2023 Volume 11

Abstract: IntroductionThe specialist Family and Community Nurse Practitioner (FCNP) is a professional who, after a period of training, is qualified to be part of multidisciplinary teams in primary care. The aim of this study was to describe and understand the ... ...

Abstract	IntroductionThe specialist Family and Community Nurse Practitioner (FCNP) is a professional who, after a period of training, is qualified to be part of multidisciplinary teams in primary care. The aim of this study was to describe and understand the experiences of nurses during their training process in the specialty of Family and Community Nursing in Spain.MethodsA descriptive qualitative study was carried out. Participants were recruited by means of convenience sampling from January to April 2022. Sixteen specialist nurses in Family and Community Nursing from different autonomous communities in Spain participated in the study. Twelve individual interviews and one focus group were conducted. Data were analyzed following a thematic analysis method in ATLAS.ti 9.ResultsThe results showed two themes and six subthemes: (1) Residency period, more than a training: (a) Training during the residency period; (b) Specializing through a constant struggle; (c) Moderate optimism about the future of the specialty; and (2) A journey from illusion to disappointment: (a) Beginning of the residency: feeling special; (b) During the residency: between satisfaction and misunderstanding; (c) At the end of the residency: power and frustration.ConclusionsThe residency period is important in the training and acquisition of competencies for the Family and Community Nurse Practitioner. Improvements are needed to ensure quality training during residency and to help give visibility to the specialty.
Keywords	primary care ; family and community nurse ; specialist nurse ; training ; experiences ; qualitative study ; Public aspects of medicine ; RA1-1270
Subject code	796
Language	English
Publishing date	2023-05-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Benefits of a Low-Cost Walking Device in Children with Cerebral Palsy

Isabel Rodríguez-Costa / Irene De la Cruz-López / Ignacio Fernández-Zárate / Saturnino Maldonado-Bascón / Sergio Lafuente-Arroyo / Susana Nunez-Nagy

International Journal of Environmental Research and Public Health, Vol 18, Iss 2808, p

A Qualitative Study

2021 Volume 2808

Abstract: Children with Cerebral Palsy (CP) participate less regularly in physical and social activities. Support walkers allow mobility for infants who need aid. The aim of this study is to explore the benefits of a low-cost walking device in children with CP. A ... ...

Abstract	Children with Cerebral Palsy (CP) participate less regularly in physical and social activities. Support walkers allow mobility for infants who need aid. The aim of this study is to explore the benefits of a low-cost walking device in children with CP. A qualitative study using semi-structured, face-to-face interviews was conducted. Eight participants (two parents, two educational professionals, and four physical therapists) who live or work with children with CP that use a low-cost walking device were questioned to examine the benefits of the practice. Thematic analysis denoted three key factors about the benefits: emotional welfare, physical wellbeing, and social enjoyment. To conclude, the use of a support walker in children with CP makes them feel happier, improves their self-confidence and autonomy, and promotes participation.
Keywords	low-cost technology ; self-help device ; cerebral palsy ; qualitative research ; Medicine ; R
Subject code	360
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Dry Needling of the Popliteus Muscle Validation by Ultrasound Imaging

José-Ignacio De-Arriba-Agre / Carmen García-Mulas / Sara Grigelmo-Hernández / Jose-Jesús Jiménez-Rejano / Samuel Fernández-Carnero / Fermin Naranjo-Cinto / Daniel Pecos-Martín / Susana Nunez-Nagy

Journal of Clinical Medicine, Vol 11, Iss 6409, p

A Cross-Sectional Observational Study

2022 Volume 6409

Abstract: Dry needling is a widely used technique for the treatment of painful syndromes in the musculature, however, its usefulness is of greater relevance in deep structures, such as the popliteus muscle, as it is more difficult to access. This muscle is heavily ...

Abstract	Dry needling is a widely used technique for the treatment of painful syndromes in the musculature, however, its usefulness is of greater relevance in deep structures, such as the popliteus muscle, as it is more difficult to access. This muscle is heavily involved in knee pathology, being a source of pain and functional impairment, especially secondary to underlying pathologies. The method selected for the observation and study of the soft tissues, by means of imaging tests that do not use ionising radiation, is ultrasound. A cross-sectional observational study is proposed. It will be carried out in a healthy population, during the years 2021 and 2022, observing, by ultrasound, the results of the popliteal puncture technique, recorded by Mayoral del Moral et al. A popliteus muscle needle reach of 92% was achieved with this technique, in 48 of 50 patients. The results of the present cross-sectional observational study in living subjects, support that the popliteal puncture, described by Mayoral et al. is a reliable and safe approach, when performed with a 0.30 × 50 mm needle, and no adverse reactions or punctures of the vascular-nerve structures have been reported during the interventions.
Keywords	dry puncture ; popliteal muscle ; ultrasound ; Medicine ; R
Subject code	616
Language	English
Publishing date	2022-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Myofascial Trigger Points and Central Sensitization Signs, but No Anxiety, Are Shown in Women with Dysmenorrhea

Yennyt-Tatiana Hoyos-Calderon / Patricia Martínez-Merinero / Susana Nunez-Nagy / Daniel Pecos-Martín / César Calvo-Lobo / Carlos Romero-Morales / Vanesa Abuín-Porras / Ana Serrano-Imedio

Biology, Vol 11, Iss 1550, p

A Case-Control Study

2022 Volume 1550

Abstract: Background primary dysmenorrhea (PD) is considered to be a cyclic chronic pelvic pain, with its onset in menstrual periods, often accompanied by the presence of myofascial trigger points (MTP). Most MTPs in subjects with chronic pelvic pain are in the ... ...

Abstract	Background primary dysmenorrhea (PD) is considered to be a cyclic chronic pelvic pain, with its onset in menstrual periods, often accompanied by the presence of myofascial trigger points (MTP). Most MTPs in subjects with chronic pelvic pain are in the inferior part of the abdomen, in the rectus abdominis (RA) area. Central sensitization is closely related to chronic pain processes. Previous studies in women with chronic pelvic pain reported central sensitization signs in their subjects, such as lower pain pressure threshold (PPT). Several authors agree that PPT in the tibialis anterior (TA) muscle, seems to be a reliable reference for signs of central sensitization. Amongst the factors that seem to accompany central sensitization, the presence of anxiety needs to be considered. The aim of the present study was to analyze the existence of hyperalgesic MTPs in RA, central sensitization signs and anxiety in women with PD, in comparison with a control group (CG). Methods: This study was designed following an observational, cross-sectional, case-control model. A total sample of 80 subjects was recruited trough social webs and advertising (PD n = 39) (CG n = 41). PPT in RA and AT was assessed bilaterally through algometry, and anxiety was evaluated through the State–Trait Anxiety Inventory. Results: Statistically significant differences ( p < 0.001) were shown for NRS average and maximum increase, as well as lower bilaterally RA and TA PPT in favor of PD group compared to CG. State or trait STAI did not show any statistically significant differences ( p > 0.05) between groups. Conclusions: In this study, women with PD reported symptoms of myofascial pain syndrome and central sensitization, when compared with healthy controls, without any sign of anxiety acting as a confounder for pain sensitivity.
Keywords	pelvic floor ; dysmenorrhea ; pain ; Biology (General) ; QH301-705.5
Subject code	150
Language	English
Publishing date	2022-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Architectural Ultrasound Pennation Angle Measurement of Lumbar Multifidus Muscles

Cristina Monsalve-Vicente / Daniel Muñoz-Zamarro / Nicolás Cuenca-Zaldívar / Samuel Fernández-Carnero / Francisco Selva-Sarzo / Susana Nunez-Nagy / Fermin Naranjo-Cinto / Tomás Gallego-Izquierdo

Journal of Clinical Medicine, Vol 11, Iss 5174, p

A Reliability Study

2022 Volume 5174

Abstract: The pennation angle has been shown to be a relevant parameter of muscle architecture. This parameter has not previously been measured in the lumbar multifidus musculature, and it is for this reason that it has been considered of great interest to ... ...

Abstract	The pennation angle has been shown to be a relevant parameter of muscle architecture. This parameter has not previously been measured in the lumbar multifidus musculature, and it is for this reason that it has been considered of great interest to establish an assessment protocol to generate new lines of research in the future. Objective: The objective of this study was to establish a protocol for measuring the pennation angle of the multifidus muscles, with a study of intra-rater and interrater reliability values. Design: This was a reliability study following the recommendations of the Guidelines for Reporting Reliability and Agreement Studies (GRRAS). Setting: The study was carried out at University of Alcalá, Department of Physiotherapy. Subjects: Twenty-seven subjects aged between 18 and 55 years were recruited for this study. Methods: Different ultrasound images of the lumbar multifidus musculature were captured. Subsequently, with the help of ImageJ software, the pennation angle of this musculature was measured. Finally, a complex statistical analysis determined the intra- and interrater reliability. Results: The intra-rater reliability of the pennation angle measurement protocol was excellent for observer 1 in the measurement of the left-sided superficial multifidus 0.851 (0.74, 0.923), and for observer 2 in the measurement of the right-sided superficial 0.711 (0.535, 0.843) and deep multifidus 0.886 (0.798, 0.942). Interrater reliability was moderate to poor, and correlation analysis results were high for thickness vs. pennation angle. Conclusions: The designed protocol for ultrasound measurement of the pennation angle of the lumbar multifidus musculature has excellent intra-rater reliability values, supporting the main conclusions and interpretations. Normative ranges of pennation angles are reported. High correlation between variables is described.
Keywords	ultrasound ; back muscles ; pennation angle ; reliability ; Medicine ; R
Subject code	600
Language	English
Publishing date	2022-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Caracterización de la problemática del uso terapéutico del Aceite de Cannabis en Córdoba, Argentina

Pablo Gabriel Manzo / Sandra Martín / Sonia Uema / Germán Charles / Felipe Montero Bruni / Susana Núñez Montoya / María Eugenia Bertotto / Martín Eynard / Pedro Armando / Carolina Bustos Fierro

Revista de la Facultad de Ciencias Médicas de Córdoba, Vol 79, Iss

2022 Volume 2

Abstract: El uso terapéutico del aceite de “cannabis” es una problemática social que pone en tensión aspectos legales, sanitarios, científicos y culturales. La dificultad en el acceso genera un vacío aprovechado por el mercado ilegal, al que recurren pacientes y ... ...

Abstract	El uso terapéutico del aceite de “cannabis” es una problemática social que pone en tensión aspectos legales, sanitarios, científicos y culturales. La dificultad en el acceso genera un vacío aprovechado por el mercado ilegal, al que recurren pacientes y familiares para mejorar su salud y calidad de vida. Estos aceites, de composición química desconocida, se emplean sin un seguimiento terapéutico. Un equipo interdisciplinario de la UNC se involucró en esta problemática con el objetivo de aportar elementos para su caracterización en nuestro medio. Metodología: Estudio observacional-descriptivo y transversal (aprobado por el CIEIS-HNC-UNC): entrevistas a pacientes/cuidadores mayores de edad que usaban el aceite (2019). Estudio experimental: análisis de muestras de aceites de los entrevistados para determinar su contenido de cannabinoides (THC y CBD), mediante HPLC. Resultados: Se realizaron 37 entrevistas y analizaron 48 muestras. El 73% fueron adultos y adultos-mayores. El 79% empiezan a usar el aceite por recomendación de parientes/amigos o por iniciativa propia, principalmente por falta de efectividad de otras terapias (54%) y por búsqueda de alternativas (33%). El 84% lo percibe efectivo (moderado-muy efectivo) y el 78% no manifestó eventos adversos. Usos principales: epilepsia refractaria 27% y artritis/artrosis 24%. El 15% de las muestras no presentaron contenidos cuantificables de CBD y THC, y el 67% presentó THC sin CBD. El contenido de cannabinoides cuantificables fue muy bajo. Conclusiones: Se obtuvo una aproximación sobre el uso terapéutico del aceite de “cannabis” en Córdoba y su calidad. Se observó discrepancia entre la efectividad percibida y el bajo contenido de cannabinoides detectados.
Keywords	dronabinol ; cannabidiol ; cannabinoides (uso terapéutico) ; Medicine ; R ; Medicine (General) ; R5-920
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	Universidad Nacional de Córdoba
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Real versus Sham Manual Therapy in Addition to Therapeutic Exercise in the Treatment of Non-Specific Shoulder Pain

Fermin Naranjo-Cinto / Adriana-Imelda Cerón-Cordero / Claudia Figueroa-Padilla / Dulce Galindo-Paz / Samuel Fernández-Carnero / Tomás Gallego-Izquierdo / Susana Nuñez-Nagy / Daniel Pecos-Martín

Journal of Clinical Medicine, Vol 11, Iss 4395, p

A Randomized Controlled Trial

2022 Volume 4395

Abstract: The aim of this study was to evaluate if manual therapy added to a therapeutic exercise program produced greater improvements than a sham manual therapy added to same exercise program in patients with non-specific shoulder pain. This was an evaluator- ... ...

Abstract	The aim of this study was to evaluate if manual therapy added to a therapeutic exercise program produced greater improvements than a sham manual therapy added to same exercise program in patients with non-specific shoulder pain. This was an evaluator-blinded randomized controlled trial. Forty-five subjects were randomly allocated into one of three groups: manual therapy (glenohumeral mobilization technique and rib-cage technique); thoracic sham manual therapy (glenohumeral mobilization technique and rib-cage sham technique); or sham manual therapy (sham glenohumeral mobilization technique and rib-cage sham technique). All groups also received a therapeutic exercise program. Pain intensity, disability and pain-free active shoulder range of motion were measured post treatment and at 4-week and 12-week follow-ups. Mixed-model analyses of variance and post hoc pairwise comparisons with Bonferroni corrections were constructed for the analysis of the outcome measures. All groups reported improved pain intensity, disability and pain-free active shoulder range of motion. However, there were no between-group differences in these outcome measures. The addition of the manual therapy techniques applied in the present study to a therapeutic exercise protocol did not seem to add benefits to the management of subjects with non-specific shoulder pain.
Keywords	shoulder ; rotator cuff ; musculoskeletal manipulations ; exercise ; Medicine ; R
Subject code	796
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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