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  1. Article ; Online: Seasonal variation in essential oil content and chemical profile of mint in southeast of Brazil

    Joyce Pereira Alvarenga / Andreísa Flores Braga / Fernanda Ventorim Pacheco / Alexandre Alves de Carvalho / José Eduardo Brasil Pereira Pinto / Suzan Kelly Vilela Bertolucci

    Ciência Rural, Vol 51, Iss

    2021  Volume 11

    Abstract: ABSTRACT: This study evaluated the influence of seasonality in essential oil (EO) chemical composition of Mentha x piperita L. and Mentha viridis L. grown in southeast of Brazil. Leaves were collected from November 2011 until August 2013, in the middle ... ...

    Abstract ABSTRACT: This study evaluated the influence of seasonality in essential oil (EO) chemical composition of Mentha x piperita L. and Mentha viridis L. grown in southeast of Brazil. Leaves were collected from November 2011 until August 2013, in the middle of the following seasons: Spring (November), Summer (February), Autumn (May) and Winter (August). EO extraction was carried out by hydrodistillation in Clevenger apparatus for 1 hour. Chemical composition of EO was analyzed by GC-FID and GC-MS. The EO content and its chemical composition were influenced by seasonality in both species. The highest EO content was obtained for M. x piperita during spring (4.26%) and for M. viridis during spring (3.30%) and summer (3.70%). Mentha x piperita increased menthol (16.31 to 41.26%), neomenthol (3.02 to 6.39%) and menthone (5.56 to 41.58%) contents during spring and summer, respectively. Whereas, M. viridis EO content did not show a quality response pattern in relation to seasonality. Therefore, harvest time for M. x piperita is recommended during spring, and for M. viridis is recommended during spring and summer.
    Keywords medicinal plant ; Mentha x piperita ; Mentha viridis ; menthol ; linalool ; Agriculture ; S ; Agriculture (General) ; S1-972
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher Universidade Federal de Santa Maria
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Light intensities alter growth and essential oil of patchouli under shade nets

    Aurislaine Santos Ribeiro / Suzan Kelly Vilela Bertolucci / Alexandre Alves de Carvalho / Wesley Naves Tostes / Adriane Duarte Coelho / José Eduardo Brasil Pereira Pinto

    Ciência Rural, Vol 52, Iss

    2021  Volume 5

    Abstract: ABSTRACT: Pogostemon cablin (Blanco) Benth. is an aromatic species popularly known as patchouli. The essential oil rich in patchoulol extracted from leaves is used by the pharmaceutical industries. The objective was to investigate the effects of shade ... ...

    Abstract ABSTRACT: Pogostemon cablin (Blanco) Benth. is an aromatic species popularly known as patchouli. The essential oil rich in patchoulol extracted from leaves is used by the pharmaceutical industries. The objective was to investigate the effects of shade nets and shading intensities on P. cablin growth, anatomy, chemical composition and essential oil content. The experiment was conducted with two types of shade nets (black and Aluminet), three light intensities (30, 50, and 70% shading) and full sun. The different light intensities influenced the growth and the essential oil yield, and chemical composition. Patchouli plants grown in an environment with shading showed higher values for the leaf, stem, root ant total dry weights compared to full sun. At 50% of shading, Aluminet provided gain in stem and total dry weights. Aluminet and black net at 50 and 70% of shading enhance essential oil yield. Patchoulol and pogostol contents were higher under full sun, and, black net and Aluminet at 50% shading. The environment for patchouli cultivation can be improved by use shade nets, especially with Aluminet at 50% of shading.
    Keywords anatomy ; light radiation ; Pogostemon cablin ; patchoulol ; volatile compounds ; Agriculture ; S ; Agriculture (General) ; S1-972
    Subject code 580
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Universidade Federal de Santa Maria
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Determination of the phenolic, antioxidant and antimicrobial potential of leaf extracts of Pereskia grandifolia Haw

    Nelma Ferreira de Paula Vicente / Heloísa Helena de Abreu Martins / Marina Leopoldina Lamounier Campidelli / Diogo Mendes da Silva / Smail Aazza / Ellen Cristina de Souza / Suzan Kelly Vilela Bertolucci / Roberta Hilsdorf Piccoli

    Research, Society and Development, Vol 9, Iss

    2020  Volume 10

    Abstract: Pesquisas em Plantas Alimentícias Não Convencionais (PANC) quanto à presença de compostos bioativos e antimicrobianos são escassas e a ampliação do conhecimento a respeito de suas propriedades torna-se necessária. Neste estudo, quatro tipos diferentes de ...

    Abstract Pesquisas em Plantas Alimentícias Não Convencionais (PANC) quanto à presença de compostos bioativos e antimicrobianos são escassas e a ampliação do conhecimento a respeito de suas propriedades torna-se necessária. Neste estudo, quatro tipos diferentes de extratos (aquosos e hidroalcoólicos obtidos por refluxo e sonicação) de ora-pro-nóbis (Pereskia grandifolia Haw) foram investigados quanto à presença de compostos fenólicos, antioxidantes e antibacterianos. Os extratos de Pereksia grandifolia Haw obtidos por refluxo foram mais eficientes do que a sonicação, pois extraíram uma quantidade maior de compostos fenólicos, antioxidantes, flavonas e flavonóis. Em relação ao solvente, detectou-se que o hidroalcoólico permitiu uma maior quantificação de flavonóides totais (flavonas e flavonóis, flavanonas e di-hidroflavonóis) e antioxidantes (medidos pelos métodos de DPPH, molibdato e poder quelante). Quando a água foi usada como solvente extrator, maiores quantidades de compostos fenólicos totais e antioxidantes (medidos pelo DPPH e poder quelante) foram observados. Todos os extratos apresentaram atividade inibitória contra Staphylococcus aureus e Pseudomonas aeruginosa, sendo os extratos aquosos obtidos por refluxo e sonicação os que apresentaram maiores halos de inibição. Porém, quando a concentração minima bactericida foi determinada, apenas o extrato hidroalcoólico obtido por refluxo apresentou resultado efetivo nas concentrações testadas. A caracterização e quantificação dos compostos fenólicos, antioxidantes e antibacterianos de Pereskia grandifolia Haw fornecem informações importantes para aumentar o uso dessa planta, como fonte alternativa de compostos benéficos à saúde, e com potencial antimicrobiano.
    Keywords UFP ; Ora-pro-nóbis ; Bioactive compounds ; Spectrophotometry ; Pathogens. ; Special aspects of education ; LC8-6691 ; Science (General) ; Q1-390 ; Social Sciences ; H
    Language English
    Publishing date 2020-09-01T00:00:00Z
    Publisher Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Solvent Mixture Optimization in the Extraction of Bioactive Compounds and Antioxidant Activities from Garlic ( Allium sativum L.)

    Vytória Piscitelli Cavalcanti / Smail Aazza / Suzan Kelly Vilela Bertolucci / João Pedro Miranda Rocha / Adriane Duarte Coelho / Altino Júnior Mendes Oliveira / Laís Campelo Mendes / Maysa Mathias Alves Pereira / Ludmila Caproni Morais / Moacir Rossi Forim / Moacir Pasqual / Joyce Dória

    Molecules, Vol 26, Iss 6026, p

    2021  Volume 6026

    Abstract: Garlic is a health promoter that has important bioactive compounds. The bioactive extraction is an important step in the analysis of constituents present in plant preparations. The purpose of this study is to optimize the extraction with the best ... ...

    Abstract Garlic is a health promoter that has important bioactive compounds. The bioactive extraction is an important step in the analysis of constituents present in plant preparations. The purpose of this study is to optimize the extraction with the best proportion of solvents to obtain total phenolic compounds (TPC) and thiosulfinates (TS) from dried garlic powder, and evaluate the antioxidant activities of the optimized extracts. A statistical mixture simplex axial design was used to evaluate the effect of solvents (water, ethanol, and acetone), as well as mixtures of these solvents, after two ultrasound extraction cycles of 15 min. Results showed that solvent mixtures with a high portion of water and pure water were efficient for TPC and TS recovery through this extraction procedure. According to the regression model computed, the most significant solvent mixtures to obtain high TPC and TS recovery from dried garlic powder are, respectively, the binary mixture with 75% water and 25% acetone and pure water. These optimized extracts presented oxygen radical absorbance capacity. Pure water was better for total antioxidant capacity, and the binary mixture of water–acetone (75:25) was better for DPPH scavenging activity. These optimized extracts can be used for industrial and research applications.
    Keywords extraction optimization ; solvent mixtures ; response surface methodology ; phenols ; thiosulfinates ; antioxidants ; Organic chemistry ; QD241-441
    Subject code 660
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Quality and intensity of light affect Lippia gracilis Schauer plant growth and volatile compounds in vitro

    Lazzarini, Luiz Eduardo Santos / Alexandre Alves de Carvalho / Fernanda Ventorim Pacheco / Jaqueline dos Santos / José Eduardo Brasil Pereira Pinto / Sâmia Torres Silva / Suzan Kelly Vilela Bertolucci

    Plant cell, tissue, and organ culture. 2018 Dec., v. 135, no. 3

    2018  

    Abstract: The aim of this study was to evaluate the effects of different intensities and quality of light and explant type on the growth of and volatile compounds in Lippia gracilis in vitro. The treatments were as follows: light intensities of 26, 51, 69, 94, or ... ...

    Abstract The aim of this study was to evaluate the effects of different intensities and quality of light and explant type on the growth of and volatile compounds in Lippia gracilis in vitro. The treatments were as follows: light intensities of 26, 51, 69, 94, or 130 μmol m−2 s−1 from fluorescent lamps and light-emitting diode (LED) lamps at different wavelengths, namely, white, red, blue, and combinations of red and blue light at ratios of 2.5:1 and 1:2.5, respectively, and two explant types, namely, nodal and apical segments. On the 30th day of culture on half-strength Murashige and Skoog (Physiol Plant 15(3):473–497, 1962) medium, growth, production of photosynthetic pigments, chlorophyll a and b, total chlorophyll, carotenoids, and volatile constituents (using headspace gas chromatography-mass spectrometry) were analyzed. The light quality and intensity significantly influenced the in vitro growth of L. gracilis. The apical segments were superior in all parameters evaluated compared to nodal segments. The number of segments plantlet−1, root length, and leaf, shoot, root, and total weight were higher with increasing light intensity, especially under the 94 μmol m−2 s−1 treatment, for both explant types. The red light showed the highest leaf (32.28 mg plantlet−1) and total (58.33 mg plantlet−1) dry weight of all the light qualities. Major constituents, namely, ρ-cymene, γ-terpinene, thymol, carvacrol, and E-caryophyllene, were identified, regardless of light conditions. The amount and composition of volatile compounds varied according to light intensity and quality. Low intensity (26 μmol m−2 s−1) increased γ-terpinene content (12.42%) and concomitantly decreased carvacrol (38.52%). Blue LED light showed higher production of carvacrol (48.11%).
    Keywords blue light ; carotenoids ; carvacrol ; chlorophyll ; fluorescent lamps ; gamma-terpinene ; gas chromatography-mass spectrometry ; headspace analysis ; leaves ; light emitting diodes ; light intensity ; light quality ; Lippia ; photosynthesis ; pigments ; plant growth ; red light ; roots ; shoots ; thymol ; volatile compounds ; wavelengths
    Language English
    Dates of publication 2018-12
    Size p. 367-379.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 406394-6
    ISSN 1573-5044 ; 0167-6857
    ISSN (online) 1573-5044
    ISSN 0167-6857
    DOI 10.1007/s11240-018-1470-1
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum

    Monfort, Lucila Elizabeth Fragoso / Suzan Kelly Vilela Bertolucci / Andreisa Fabri Lima / Alexandre Alves de Carvalho / Aliyu Mohammed / Arie Fitzgerard Blank / José Eduardo Brasil Pereira Pinto

    Industrial crops and products. 2018 June, v. 116

    2018  

    Abstract: The influence of different culture media, strength MS media and plant growth regulators (PGR) was assessed through the growth and production of the volatile fraction composition in Ocimum basilicum (basil). The treatments were: the use of different ... ...

    Abstract The influence of different culture media, strength MS media and plant growth regulators (PGR) was assessed through the growth and production of the volatile fraction composition in Ocimum basilicum (basil). The treatments were: the use of different culture media (MS, B5 and WPM), strength MS media (2MS, MS, ½MS and ¼MS) and different PGR combinations or only IAA, NAA, IBA, BAP and TDZ. The leaf number, nodal segment, shoot number, lenght, shoot dry weight, root number, root size, root dry weight, as well as the volatile fraction analysis through Head-Space – GC/MS were assessed at the 40th culture day. The present results suggest that media type, MS concentration and the growth regulator types have qualitative and quantitatively influenced the growth and volatile composition. A higher amount of methyleugenol in the media 2MS and ¼MS, and higher content of linalool and 1,8-cineole in MS and WPM were observed. The PGR improved biomass production and induced quantitative modifications in the production of major volatile constituents in Ocimum basilicum plantlets, although the same major compounds were produced in all treatments. There was a tendency to estragole synthesis in the presence of auxin combined with cytokinin. There was a tendency to linalool synthesis in the presence of auxin isolates.
    Keywords Ocimum basilicum ; basil ; biomass production ; cineole ; culture media ; cytokinins ; gas chromatography-mass spectrometry ; headspace analysis ; indole acetic acid ; indole butyric acid ; leaves ; linalool ; methyl chavicol ; naphthaleneacetic acid ; plantlets ; volatile compounds
    Language English
    Dates of publication 2018-06
    Size p. 231-239.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1132158-1
    ISSN 1872-633X ; 0926-6690
    ISSN (online) 1872-633X
    ISSN 0926-6690
    DOI 10.1016/j.indcrop.2018.02.075
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: The growth, photosynthetic pigments and essential oil composition of monocropped and intercropped lemon balm with yarrow

    Thaís Cristina Silva / José Eduardo Brasil Pereira Pinto / Fernanda Ventorim Pacheco / Heitor Luiz Heiderich Roza / Diogo Mendes da Silva Lordêllo / Suzan Kelly Vilela Bertolucci

    Acta Scientiarum: Agronomy, Vol 40, Iss 1, Pp e35506-e

    2018  Volume 35506

    Abstract: The purpose of this study was to assess the influence of lemon balm intercropping with yarrow on the vegetative growth, photosynthetic pigments and essential oil contents as well as the chemical composition of essential oils. Accordingly, the lemon balm ... ...

    Abstract The purpose of this study was to assess the influence of lemon balm intercropping with yarrow on the vegetative growth, photosynthetic pigments and essential oil contents as well as the chemical composition of essential oils. Accordingly, the lemon balm and yarrow monocroppings were compared to the intercropping of both species. The plants were cultivated for 120 days under field conditions. The experimental design followed randomized blocks with seven repetitions. Growth was assessed through the accumulation of shoot dry matter and through the leaf area. The photosynthetic pigment content was set based on the fully expanded leaves from five plants in each treatment. The essential oil was extracted through the hydrodistillation of the lemon balm leaves and yarrow leaflets dehydrated in a forced ventilation oven at 40°C. The chemical analyses of the essential oils were performed using GC-FID and GC-MS. Intercropping showed a reduction of vegetative growth. However, intercropping increased the lemon balm essential oil content. On the other hand, these parameters did not change in yarrow. There were variations in the level of chemical constituents in lemon balm and yarrow essential oils, which were more evident in the major constituents.
    Keywords Melissa officinalis L ; Achillea millefolium L ; intercropping ; gas chromatography ; Agriculture (General) ; S1-972
    Subject code 580
    Language English
    Publishing date 2018-08-01T00:00:00Z
    Publisher Eduem (Editora da Universidade Estadual de Maringá)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article: Growth regulators affect the dry weight production, carvacrol and thymol content of Lippia gracilis Schauer

    Santos Lazzarini, Luiz Eduardo / Suzan Kelly Vilela Bertolucci / Alexandre Alves de Carvalho / Alexsandro Carvalho Santiago / Fernanda Ventorim Pacheco / Maria Mariana Ferreira Célio / José Eduardo Brasil Pereira Pinto

    Industrial crops and products. 2019 Mar., v. 129

    2019  

    Abstract: The essential oil from Lippia gracilis Schauer (Verbenaceae) leaves are rich in thymol and carvacrol with antibactericidal and antinflammatory action. The aim of the present study was to evaluate the effect of growth regulators and their combinations on ... ...

    Abstract The essential oil from Lippia gracilis Schauer (Verbenaceae) leaves are rich in thymol and carvacrol with antibactericidal and antinflammatory action. The aim of the present study was to evaluate the effect of growth regulators and their combinations on shoot proliferation, growth, and volatile fraction analysis of Lippia gracilis in vitro. In the first experiment, five different concentrations of 6-benzylaminopurine (BAP) were used: 0.0, 2.22, 3.33, 4.44, and 5.55 μM and three of naphthaleneacetic acid (NAA): 0.0, 2.68, and 5.36 μM, in a factorial design. In the second, the treatments consisted in combination of BAP + TDZ at concentration (μM): 0 + 0, 1.11 + 1.13, 1.33 + 0.91, 0.88 + 1.36, 0.43 + 1.81, and 1.78 + 0.46. At 30 days of culture in ½ Murashige and Skoog (MS) medium, the growth and volatile constituents were evaluated. Growth regulators significantly influenced in vitro growth of L. gracilis. Growth data for the first experiment were predicted from the three-dimensional (3D) response surface of the different variables analyzed. In the second experiment, the presence of growth regulators reduced shoots and root length in all combinations. The combinations among regulators (BAP and TDZ) stimulated the number of shoots per explant, but BAP and NAA induced higher shoot number. Variation in the number, content, and profile of volatile compounds were also observed under the influence of growth regulators. The major constituents ρ-cimene, γ-terpinene, thymol, carvacrol, and E-caryophyllene were identified, independent of the experimental conditions. However, the use of growth regulators significantly reduced carvacrol and thymol levels.
    Keywords Lippia ; benzyladenine ; carvacrol ; essential oils ; gamma-terpinene ; leaves ; naphthaleneacetic acid ; roots ; shoots ; thymol ; volatile compounds
    Language English
    Dates of publication 2019-03
    Size p. 35-44.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1132158-1
    ISSN 1872-633X ; 0926-6690
    ISSN (online) 1872-633X
    ISSN 0926-6690
    DOI 10.1016/j.indcrop.2018.11.070
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Organic systems in the growth and essential-oil production of the yarrow

    Elza Oliveira Ferraz / Suzan Kelly Vilela Bertolucci / José Eduardo Brasil Pereira Pinto / Andreísa Flores Braga / Andressa Giovannini Costa

    Revista Ciência Agronômica, Vol 45, Iss 1, Pp 111-

    2014  Volume 119

    Abstract: Fertilization of the soil with organic fertilizers has been gaining importance within the concept of sustainable crop production. This study aimed evaluates the effects of dosages of cattle and poultry manure on Achillea millefolium L. as regards the ... ...

    Abstract Fertilization of the soil with organic fertilizers has been gaining importance within the concept of sustainable crop production. This study aimed evaluates the effects of dosages of cattle and poultry manure on Achillea millefolium L. as regards the vegetative growth and the content and chemical composition of its essential oil. For the cattle manure fertilization the dosages evaluated were: 1) soil with no fertilizer; 2) soil + 3.0 kg m-2; 3) soil + 6.0 kg m-2; 4) soil + 9.0 kg m-2 and 5) soil + 12.0 kg m-2. For fertilization with poultry manure: 1) soil without fertilizer; 2) soil + 1.5 kg m-2; 3) soil + 3.0 kg m-2; 4) soil + 4.5 kg m-2 and 5) soil + 6.0 kg m-2. The experimental design was completely randomized, with four replications per treatment and four plants per experimental plot. Harvesting took place at 110 days and the following variables were measured: shoot and root dry biomass; root to shoot ratio and the content, yield and chemical composition of the essential oil. Data were submitted to variance and regression analyses. A. millefolium has more intense response in fertilization with poultry manure than to that of cattle, where the dosage of 6 kg m-2 presented the greatest shoot dry weight and highest yield of essential oil. Without regard to fertilization, the essential oil of A. millefolium consists mainly of chamazulene, with the applied treatments not significantly interfering in the oil chemical composition and content.
    Keywords Achillea millefolium L ; Essential oil ; Organic fertilizer ; Soil fertily ; Biomass ; Agriculture (General) ; S1-972
    Subject code 630
    Language English
    Publishing date 2014-03-01T00:00:00Z
    Publisher Universidade Federal do Ceará
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Essential oil of monkey-pepper (Piper aduncum L.) cultivated under different light environments

    Pacheco, Fernanda Ventorim / Amauri Alves de Alvarenga / Ivan Caldeira Almeida Alvarenga / José Eduardo Brasil Pereira Pinto / Rafaella de Paula Avelar / Suzan Kelly Vilela Bertolucci

    Industrial crops and products. 2016 July, v. 85

    2016  

    Abstract: The monkey-pepper (Piper aduncum L.) is an herbaceous species and essential oil producer, with biological activities such as insecticidal, antimicrobial, larvacidal and others. The cultivation of the plant is still incipient, being necessary to know the ... ...

    Abstract The monkey-pepper (Piper aduncum L.) is an herbaceous species and essential oil producer, with biological activities such as insecticidal, antimicrobial, larvacidal and others. The cultivation of the plant is still incipient, being necessary to know the influence of some factors, among them, the light. Thus, the effects of different lighting conditions on the production and profile of the constituents of essential oils from leaves and roots of Piper aduncum L. were evaluated. Plants grown for 150 days in five light conditions: 100%, 70% and 50% of natural irradiance and nets of blue and red colors, also blocking 50% of natural light irradiance. The essential oils obtained by hydrodistillation from leaves and roots and analyzed by GC/MS. Roots showed higher amount of essential oil (0.11% w/w), which had no influence on light conditions. On leaves the biggest oil content was observed in 50% brightness (0.085% w/w). The essential oil of leaves was characterized by a high content of sesquiterpenes and roots of phenylpropanoids. The leaves are rich in E-nerolidol, linalol (14.28–16.65%); α-humulene (8.45–10.62%); cis-cadin-4-en-7-ol (7.48–12.24%) and caryophyllene (7.93–12.24%). While the root is rich in apiol (16.27–29.51%). Most major compounds increased in the environment rich in blue light. The variation in the production and essential oil constituents of P. aduncum L. depends on the light conditions and can is related to phenotypic and genetic factors. Therefore control of the light environment in growing conditions makes it necessary for obtaining raw material quality.
    Keywords blue light ; color ; essential oils ; hydrodistillation ; insecticidal properties ; leaves ; light intensity ; lighting ; linalool ; lipid content ; phenotype ; phenylpropanoids ; Piper aduncum ; raw materials ; roots ; sesquiterpenoids ; solar radiation
    Language English
    Dates of publication 2016-07
    Size p. 251-257.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1132158-1
    ISSN 1872-633X ; 0926-6690
    ISSN (online) 1872-633X
    ISSN 0926-6690
    DOI 10.1016/j.indcrop.2016.03.016
    Database NAL-Catalogue (AGRICOLA)

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