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  1. Article: [Current Studies of Staphylococcal Food Poisoning].

    Suzuki, Yasunori

    Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan

    2019  Volume 60, Issue 3, Page(s) 27–37

    MeSH term(s) Disease Outbreaks ; Food Contamination ; Humans ; Polymerase Chain Reaction ; Staphylococcal Food Poisoning
    Language Japanese
    Publishing date 2019-08-07
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 604314-8
    ISSN 1882-1006 ; 0015-6426
    ISSN (online) 1882-1006
    ISSN 0015-6426
    DOI 10.3358/shokueishi.60.27
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Nephrolithiasis and/or nephrocalcinosis is significantly related to renal dysfunction in patients with primary Sjögren's syndrome.

    Fujisawa, Yuhei / Mizushima, Ichiro / Suzuki, Yasunori / Kawano, Mitsuhiro

    Modern rheumatology

    2023  Volume 34, Issue 2, Page(s) 376–381

    Abstract: Objective: The present study compared the clinical features of patients with primary Sjögren's syndrome (pSS) with and without nephrolithiasis and/or nephrocalcinosis to determine factors related to renal dysfunction.: Methods: The clinical features ... ...

    Abstract Objective: The present study compared the clinical features of patients with primary Sjögren's syndrome (pSS) with and without nephrolithiasis and/or nephrocalcinosis to determine factors related to renal dysfunction.
    Methods: The clinical features of 68 patients with anti-Sjogren's syndrome antigen A (SSA)/Ro-antibody-positive pSS with and without nephrolithiasis and/or nephrocalcinosis who underwent abdominal computed tomography and/or ultrasonography were retrospectively analysed.
    Results: Of the 68 patients with anti-SSA-antibody-positive pSS, 23 (33%) had renal nephrolithiasis and/or nephrocalcinosis, whereas 45 (67%) did not. Fourteen (20%) patients had renal dysfunction at diagnostic imaging. Among five patients who underwent renal biopsy, four patients with renal nephrolithiasis and/or nephrocalcinosis were diagnosed with tubulointerstitial nephritis, and one without nephrolithiasis and/or nephrocalcinosis was diagnosed with minimal change nephrotic syndrome. Estimated glomerular filtration rate at diagnostic imaging was significantly lower in patients with than without nephrolithiasis and/or nephrocalcinosis group (P = 0.010). In addition to nephrolithiasis and/or nephrocalcinosis (odds ratio [OR], 3.467; P = 0.045), the gap between serum sodium and chloride concentrations (OR, 10.400; P = 0.012) and increased urinary β2-microglobulin (OR, 5.444; P = 0.033) were associated with renal dysfunction at the time of diagnostic imaging.
    Conclusion: Nephrolithiasis and/or nephrocalcinosis, normal anion gap metabolic acidosis, and tubulointerstitial damage are associated with renal dysfunction in patients with pSS.
    MeSH term(s) Humans ; Nephrocalcinosis/complications ; Nephrocalcinosis/diagnostic imaging ; Sjogren's Syndrome/complications ; Sjogren's Syndrome/diagnosis ; Retrospective Studies ; Acidosis, Renal Tubular/complications ; Nephrolithiasis/complications ; Nephrolithiasis/diagnostic imaging ; Antibodies
    Chemical Substances Antibodies
    Language English
    Publishing date 2023-07-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 2078157-X
    ISSN 1439-7609 ; 1439-7595
    ISSN (online) 1439-7609
    ISSN 1439-7595
    DOI 10.1093/mr/road023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Humoral response after BNT162b2 vaccine in Japanese hemodialysis patients.

    Miyazaki, Ryoichi / Miyagi, Kyoko / Yoshida, Misaki / Suzuki, Yasunori

    Renal replacement therapy

    2023  Volume 9, Issue 1, Page(s) 13

    Abstract: Background: Hemodialysis patients are more likely to be severely affected if infected by COVID-19. Contributing factors include chronic kidney disease, old age, hypertension, type 2 diabetes, heart disease, and cerebrovascular disease. Therefore, action ...

    Abstract Background: Hemodialysis patients are more likely to be severely affected if infected by COVID-19. Contributing factors include chronic kidney disease, old age, hypertension, type 2 diabetes, heart disease, and cerebrovascular disease. Therefore, action against COVID-19 for hemodialysis patients is an urgent issue. Vaccines are effective in preventing COVID 19 infection. In hemodialysis patients, however, responses to hepatitis B and influenza vaccines are reportedly weak. The BNT162b2 vaccine has shown an efficacy rate of about 95% in the general population, but as far as we know there are only several reports of efficacy data in hemodialysis patients in Japan.
    Methods: We assessed serum anti-SARS-CoV-2 IgG antibody (Abbott SARS-CoV-2 IgG II Quan) in 185 hemodialysis patients and 109 health care workers. The exclusion criterion was positivity for SARS-CoV-2 IgG antibody before vaccination. Adverse reactions to BNT162b2 vaccine were evaluated through interviews.
    Results: Following vaccination, 97.6% of the hemodialysis group and 100% of the control group were positive for the anti-spike antibody. The median level of anti-spike antibody was 2,728.7 AU/mL (IQR, 1,024.2-7,688.2 AU/mL) in the hemodialysis group and 10,500 AU/ml (IQR, 9,346.1-2,4500 AU/mL) in the health care workers group. The factors involved in the low response to the BNT152b2 vaccine included old age, low BMI, low Cr index, low nPCR, low GNRI, low lymphocyte count, steroid administration, and complications related to blood disorders.
    Conclusions: Humoral responses to BNT162b2 vaccine in hemodialysis patients are weaker than in a healthy control sample. Booster vaccination is necessary for hemodialysis patients, especially those showing a weak or non-response to the two-dose BNT162b2 vaccine.
    Language English
    Publishing date 2023-02-20
    Publishing country England
    Document type Journal Article
    ISSN 2059-1381
    ISSN (online) 2059-1381
    DOI 10.1186/s41100-022-00452-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Pediatric IgG4-related disease: a descriptive review.

    Hara, Satoshi / Yoshida, Misaki / Sanada, Hajime / Suzuki, Yasunori / Sato, Yasuharu / Mizushima, Ichiro / Kawano, Mitsuhiro

    Expert review of clinical immunology

    2024  Volume 20, Issue 1, Page(s) 97–119

    Abstract: Introduction: IgG4-related disease (IgG4-RD) is an immune-mediated systemic fibroinflammatory condition characterized by serum IgG4 elevation and IgG4-positive plasma cell infiltration into various organs. It generally occurs in elderly males. Pediatric ...

    Abstract Introduction: IgG4-related disease (IgG4-RD) is an immune-mediated systemic fibroinflammatory condition characterized by serum IgG4 elevation and IgG4-positive plasma cell infiltration into various organs. It generally occurs in elderly males. Pediatric cases have been reported, albeit rarely, accordingly lack of recognition of such cases could delay therapeutic intervention leading to poorer outcomes.
    Areas covered: The present review is a descriptive review of all published case reports, cohort studies, and reviews of pediatric IgG4-RD listed in PubMed. Characteristics of pediatric IgG4-RD were clarified, including sex, organ involvement, serological and histological findings, and treatment. We assessed how many published cases met current classification and comprehensive diagnostic criteria.
    Expert opinion: The characteristics of pediatricIgG4-RD differed from adult IgG4-RD in terms of sex and involved organs. There was no clear male dominance in numbers of cases, and surface organ involvement such as ophthalmic diseases were more common in the pediatric IgG4-RD. Organ involvement tended to be indolent and unilateral, causing difficulty in definitively diagnosing pediatric IgG4-RD. Only about 20% of published cases met IgG4-RD classification or comprehensive diagnostic criteria. Physicians should be careful in diagnosing pediatric IgG4-RD after excluding mimickers. International collaboration toward high-quality evidence to support diagnosis and treatment of pediatric IgG4-RD is advised.
    MeSH term(s) Adult ; Humans ; Male ; Child ; Aged ; Immunoglobulin G4-Related Disease/diagnosis ; Autoimmune Diseases/diagnosis ; Immunoglobulin G ; Cohort Studies ; Diagnosis, Differential
    Chemical Substances Immunoglobulin G
    Language English
    Publishing date 2024-01-08
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2274260-8
    ISSN 1744-8409 ; 1744-666X
    ISSN (online) 1744-8409
    ISSN 1744-666X
    DOI 10.1080/1744666X.2023.2274358
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: [Rhodococcus equi infections in humans: an emerging zoonotic pathogen].

    Takai, Shinji / Mizuno, Yasutaka / Suzuki, Yasunori / Sasaki, Yukako / Kakuda, Tsutomu / Kirikae, Teruo

    Nihon saikingaku zasshi. Japanese journal of bacteriology

    2024  Volume 79, Issue 1, Page(s) 15–24

    Abstract: Rhodococcus equi is a facultative intracellular gram-positive coccobacillus which is a well-known cause of foal pneumonia and/or enteritis in equine veterinary medicine. More than 300 cases of R. equi infection have been reported since the first ... ...

    Abstract Rhodococcus equi is a facultative intracellular gram-positive coccobacillus which is a well-known cause of foal pneumonia and/or enteritis in equine veterinary medicine. More than 300 cases of R. equi infection have been reported since the first description of human disease in 1968. Most patients who become infected with R equi are immunocompromised, such as those infected with human immunodeficiency virus (HIV), recipients of organ transplantation, and patients receiving cancer treatment. However, there are increasing reports of the immunocompetent hosts. The pathogenicity of R. equi has been attributed to the presence of plasmid-encoded virulence-associated proteins (Vap). To date, three host-associated virulence plasmid types of R. equi have been identified as follows: the circular pVAPA and pVAPB, related, respectively, to equine and porcine isolates in 1991 and 1995, and a recently described linear pVAPN plasmid associated with bovine and caprine strains in 2015. More recently, these three plasmid types have been re-found in the human isolates which were isolated during 1980s to 1990s. Not only horses, but also pigs, goats, cattle and their environment should be considered as a potential source of R. equi for humans. In this review, we shed light on the current understanding of R. equi as an emerging zoonotic pathogen.
    MeSH term(s) Humans ; Animals ; Horses ; Cattle ; Swine ; Virulence Factors/genetics ; Rhodococcus equi/genetics ; Goats ; Plasmids/genetics
    Chemical Substances Virulence Factors
    Language Japanese
    Publishing date 2024-02-20
    Publishing country Japan
    Document type Review ; English Abstract ; Journal Article
    ZDB-ID 604101-2
    ISSN 1882-4110 ; 0021-4930
    ISSN (online) 1882-4110
    ISSN 0021-4930
    DOI 10.3412/jsb.79.15
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Antitumoral RNA-targeted oligonucleotide therapeutics: The third pillar after small molecule inhibitors and antibodies.

    Taniguchi, Hiroaki / Suzuki, Yasunori / Imai, Kohzoh / Adachi, Yasushi

    Cancer science

    2022  Volume 113, Issue 9, Page(s) 2952–2961

    Abstract: Oligonucleotide therapeutics, drugs consisting of 10-50 nucleotide-long single- or double-stranded DNA or RNA molecules that can bind to specific DNA or RNA sequences or proteins, include antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), ...

    Abstract Oligonucleotide therapeutics, drugs consisting of 10-50 nucleotide-long single- or double-stranded DNA or RNA molecules that can bind to specific DNA or RNA sequences or proteins, include antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), microRNAs (miRNAs), aptamers, and decoys. These oligonucleotide therapeutics could potentially become the third pillar of drug development. In particular, ASOs and siRNAs are advanced tools that are widely used to silence gene expression. They are used in clinical trials, as they have high specificity for target mRNAs and non-coding RNAs and limited toxicity. However, their clinical application remains challenging. Although chemotherapy has benefits, it has severe adverse effects in many patients. Therefore, new modalities for targeted molecular therapy against tumors, including oligonucleotide therapeutics, are required, and they should be compatible with diagnosis using next-generation sequencing. This review provides an overview of the therapeutic uses of ASOs, siRNAs, and miRNAs in clinical studies on malignant tumors. Understanding previous research and development will help in developing novel oligonucleotide therapeutics against malignant tumors.
    MeSH term(s) Antibodies ; Humans ; MicroRNAs/genetics ; Neoplasms/drug therapy ; Neoplasms/genetics ; Oligonucleotides/pharmacology ; Oligonucleotides/therapeutic use ; Oligonucleotides, Antisense/genetics ; Oligonucleotides, Antisense/pharmacology ; Oligonucleotides, Antisense/therapeutic use ; RNA, Small Interfering/genetics ; RNA, Small Interfering/pharmacology ; RNA, Small Interfering/therapeutic use
    Chemical Substances Antibodies ; MicroRNAs ; Oligonucleotides ; Oligonucleotides, Antisense ; RNA, Small Interfering
    Language English
    Publishing date 2022-07-11
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2115647-5
    ISSN 1349-7006 ; 1349-7006
    ISSN (online) 1349-7006
    ISSN 1349-7006
    DOI 10.1111/cas.15461
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  7. Article ; Online: Isolation of vapB-positive Rhodococcus equi from submaxillary lymph nodes with or without granulomatous lesions in growing-finishing pigs in Japan.

    Matsuoka, Michinori / Kobayashi, Ayumi / Matsuoka, Misa / Honda, Yayoi / Hashimoto, Riri / Sasaki, Yukako / Kakuda, Tsutomu / Suzuki, Yasunori / Takai, Shinji

    The Journal of veterinary medical science

    2024  

    Abstract: To investigate the etiological role of vapB-positive Rhodococcus equi in pigs, R. equi was isolated from the submaxillary lymph nodes with or without macroscopically detectable lesions of apparently healthy growing-finishing pigs at a slaughterhouse in ... ...

    Abstract To investigate the etiological role of vapB-positive Rhodococcus equi in pigs, R. equi was isolated from the submaxillary lymph nodes with or without macroscopically detectable lesions of apparently healthy growing-finishing pigs at a slaughterhouse in Toyama Prefecture, Japan. R. equi was isolated from 57 (24.6%) of 232 pigs with macroscopically detectable lymph node lesions, and 56 (98.2%) of the 57 isolates were vapB-positive. R. equi was isolated from 10 (2.4%) of 420 pigs without lymph node lesions, and six (60%) of the 10 isolates were vapB-positive. Plasmid DNA was isolated from the 62 vapB-positive isolates and digested with EcoRI and NsiI to obtain the plasmid profile. Fifty-two (83.9%), three (4.8%), and four (6.5%) isolates contained pVAPB subtypes 1, 2, and 3, respectively, while the remaining three isolates were of pVAPB subtypes 9, 13, and 14, respectively. Twelve specimens from lymph nodes with macroscopically detectable lesions were randomly selected for histopathological staining. Granulomatous lesions resembling tuberculosis were found in 11 of the 12 specimens, and the remaining specimen showed typical foci of malakoplakia in the lymph node. The isolation rates of R. equi and vapB-positive R. equi from lymph nodes with macroscopically detectable lesions were significantly higher (P<0.05) than those of lymph nodes without lesions, suggesting an etiologic association between vapB-positive R. equi and macroscopically detectable granulomatous lesions in porcine submaxillary lymph nodes. Previous reports on the prevalence of vapB-positive R. equi in pigs are reviewed and discussed.
    Language English
    Publishing date 2024-04-16
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 1071753-5
    ISSN 1347-7439 ; 0916-7250
    ISSN (online) 1347-7439
    ISSN 0916-7250
    DOI 10.1292/jvms.24-0067
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Birth month associated with tracheal colonization of Rhodococcus equi in newborn foals on horse-breeding farms with sporadic rhodococcosis in Japan

    Takai, Shinji / Yoda, Ayaka / Sasaki, Yukako / Kakuda, Tsutomu / Suzuki, Yasunori / Oikawa, Masaaki

    Veterinary microbiology. 2022 Apr., v. 267

    2022  

    Abstract: Tracheal washing fluid was collected from 170 foals at 28 and 35 d old from February to July in a foaling season on horse-breeding farms with sporadic rhodococcosis in Japan and was investigated by quantitative culture. The history of the 170 foals ... ...

    Abstract Tracheal washing fluid was collected from 170 foals at 28 and 35 d old from February to July in a foaling season on horse-breeding farms with sporadic rhodococcosis in Japan and was investigated by quantitative culture. The history of the 170 foals followed up for the next few months. The proportion of R. equi-positive foals at 28 and 35 d old was significantly increased according to the birth month. Furthermore, the mean number of R. equi in the tracheal washing fluid of each month group increased according to their birth month with the rise in outside temperature. During the follow-up observation, 9/30 foals (30.0 %) born in February showed the first clinical signs at 56 ± 8 d old, 21/61 foals (34.4 %) born in March showed the signs at 37 ± 3 d old, 15/49 foals (30.6 %) born in April showed the signs at 39 ± 2 d old, and 7/30 foals (23.3 %) born in May showed signs at 44 ± 3 d old. Two sick foals (6.7 %) born in February, 19 sick foals (31.1 %) born in March, 15 sick foals (30.6 %) born in April, and 6 sick foals (20.0 %) born in May showed a positive culture of R. equi at 28 or 35 d old. The present study revealed that birth month is associated with the initial colonization of R. equi in the trachea of newborn foals on farms with sporadic rhodococcosis in Japan. Therefore, birth month might be a risk factor for developing R. equi pneumonia in foals.
    Keywords Japan ; Rhodococcus equi ; microbiology ; neonates ; pneumonia ; risk factors ; temperature
    Language English
    Dates of publication 2022-04
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2022.109373
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Complete Genome Sequences of Staphylococcus argenteus Tokyo13064 and Tokyo13069, Isolated from Specimens Obtained during a Food Poisoning Outbreak in Tokyo, Japan.

    Suzuki, Yasunori / Kubota, Hiroaki / Kakuda, Tsutomu / Takai, Shinji / Sadamasu, Kenji

    Microbiology resource announcements

    2021  Volume 10, Issue 10

    Abstract: The complete genome sequences of ... ...

    Abstract The complete genome sequences of two
    Language English
    Publishing date 2021-03-11
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.01447-20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Identification of genes required for the fitness of Rhodococcus equi during the infection of mice via signature-tagged transposon mutagenesis.

    Sangkanjanavanich, Nuttapone / Kakuda, Tsutomu / Suzuki, Yasunori / Sasaki, Yukako / Takai, Shinji

    The Journal of veterinary medical science

    2021  Volume 83, Issue 8, Page(s) 1182–1190

    Abstract: Rhodococcus equi is a Gram-positive facultative intracellular bacterium that causes pyogranulomatous pneumonia in foals and immunocompromised people. In the present study, signature-tagged transposon mutagenesis was applied for the negative selection of ... ...

    Abstract Rhodococcus equi is a Gram-positive facultative intracellular bacterium that causes pyogranulomatous pneumonia in foals and immunocompromised people. In the present study, signature-tagged transposon mutagenesis was applied for the negative selection of R. equi mutants that cannot survive in vivo. Twenty-five distinguishable plasmid-transposon (plasposon) vectors by polymerase chain reaction (PCR), each containing a unique oligonucleotide tag, were constructed and used to select the transposon mutants that have in vivo fitness defects using a mouse systemic infection model. Of the 4,560 transposon mutants, 102 mutants were isolated via a real-time PCR-based screening as the mutants were unable to survive in the mouse model. Finally, 50 single transposon insertion sites were determined via the self-cloning strategy. The insertion of the transposon was seen on the virulence plasmid in 15 of the 50 mutants, whereas the remaining 35 mutants had the insertion of transposon on the chromosome. The chromosomal mutants contained transposon insertions in genes involved in cellular metabolism, DNA repair and recombination, gene regulation, non-ribosomal peptide synthesis, and unknown functions. Additionally, seven of the chromosomal mutants showed a reduced ability to multiply in the macrophages in vitro. In this study, we have identified several biosynthetic pathways as fitness factors associated with the growth within macrophages and survival in mice.
    MeSH term(s) Animals ; DNA Transposable Elements/genetics ; Horses ; Mutagenesis ; Plasmids/genetics ; Rhodococcus ; Rhodococcus equi/genetics ; Virulence/genetics
    Chemical Substances DNA Transposable Elements
    Language English
    Publishing date 2021-06-10
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 1071753-5
    ISSN 1347-7439 ; 0916-7250
    ISSN (online) 1347-7439
    ISSN 0916-7250
    DOI 10.1292/jvms.21-0256
    Database MEDical Literature Analysis and Retrieval System OnLINE

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