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Article ; Online: Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition

Wenguang G. Liang / Juwina Wijaya / Hui Wei / Alex J. Noble / Jordan M. Mancl / Swansea Mo / David Lee / John V. Lin King / Man Pan / Chang Liu / Carla M. Koehler / Minglei Zhao / Clinton S. Potter / Bridget Carragher / Sheng Li / Wei-Jen Tang

Nature Communications, Vol 13, Iss 1, Pp 1-

2022 Volume 14

Abstract: Presequence protease (PreP) is essential to mitochondrial proteostasis. This study leverages advanced vitrification techniques to solve cryoEM structures of apo- and substrate-bound PreP and integrates these data with other analysis to reveal key stages ... ...

Abstract	Presequence protease (PreP) is essential to mitochondrial proteostasis. This study leverages advanced vitrification techniques to solve cryoEM structures of apo- and substrate-bound PreP and integrates these data with other analysis to reveal key stages and mechanistic insights of the PreP catalytic cycle.
Keywords	Science ; Q
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition.

Liang, Wenguang G / Wijaya, Juwina / Wei, Hui / Noble, Alex J / Mancl, Jordan M / Mo, Swansea / Lee, David / Lin King, John V / Pan, Man / Liu, Chang / Koehler, Carla M / Zhao, Minglei / Potter, Clinton S / Carragher, Bridget / Li, Sheng / Tang, Wei-Jen

Nature communications

2022 Volume 13, Issue 1, Page(s) 1833

Abstract: Presequence protease (PreP), a 117 kDa mitochondrial M16C metalloprotease vital for mitochondrial proteostasis, degrades presequence peptides cleaved off from nuclear-encoded proteins and other aggregation-prone peptides, such as amyloid β (Aβ). PreP ... ...

Abstract	Presequence protease (PreP), a 117 kDa mitochondrial M16C metalloprotease vital for mitochondrial proteostasis, degrades presequence peptides cleaved off from nuclear-encoded proteins and other aggregation-prone peptides, such as amyloid β (Aβ). PreP structures have only been determined in a closed conformation; thus, the mechanisms of substrate binding and selectivity remain elusive. Here, we leverage advanced vitrification techniques to overcome the preferential denaturation of one of two ~55 kDa homologous domains of PreP caused by air-water interface adsorption. Thereby, we elucidate cryoEM structures of three apo-PreP open states along with Aβ- and citrate synthase presequence-bound PreP at 3.3-4.6 Å resolution. Together with integrative biophysical and pharmacological approaches, these structures reveal the key stages of the PreP catalytic cycle and how the binding of substrates or PreP inhibitor drives a rigid body motion of the protein for substrate binding and catalysis. Together, our studies provide key mechanistic insights into M16C metalloproteases for future therapeutic innovations.
MeSH term(s)	Amyloid beta-Peptides/metabolism ; Cryoelectron Microscopy ; Humans ; Metalloproteases/metabolism ; Mitochondria/metabolism ; Molecular Conformation ; Protein Conformation ; Substrate Specificity
Chemical Substances	Amyloid beta-Peptides ; Metalloproteases (EC 3.4.-)
Language	English
Publishing date	2022-04-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-022-29322-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online: Issues in History Teaching

Arthur, James / ROBERT PHILLIPS, UNIVERSITY OF WALES SWANSEA

(Issues in Teaching Series)

2012

Abstract: Written by a range of history professionals, including HMIs, this book provides excellent ideas on the teaching, learning and organization of history in primary and secondary ... ...

Series title	Issues in Teaching Series
Abstract	Written by a range of history professionals, including HMIs, this book provides excellent ideas on the teaching, learning and organization of history in primary and secondary schools
Language	English
Size	Online-Ressource (260 p)
Publisher	Taylor and Francis
Publishing place	Hoboken
Document type	Book ; Online
Note	Description based upon print version of record
ISBN	9780415206686 ; 0415206685
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Article: Regenerated extracellular NH4+ affects the motile chemosensory responses of batch-cultured Oxyrrhis marina

Martel, Claire M.(Swansea University School of Medicine Institute of Life Science)

Brazilian Journal of Microbiology

2010/06

Abstract: Regenerated extracellular NH4+in laboratory batch-cultures of the heterotrophic marine microzooplankter Oxyrrhis marina affects the strength and consistency of chemotaxes elicited by synthetic and biogenic chemoattractants. The ecological relevance of ... ...

Abstract	Regenerated extracellular NH4+in laboratory batch-cultures of the heterotrophic marine microzooplankter Oxyrrhis marina affects the strength and consistency of chemotaxes elicited by synthetic and biogenic chemoattractants. The ecological relevance of experiments with batch-cultured O. marina and limitations of the microcapillary assay for the study of chemosensory behaviours are discussed.
Language	English
Document type	Article
ISSN	1517-8382
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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Article ; Online: Nurse-led medicines' monitoring in care homes, implementing the Adverse Drug Reaction (ADRe) Profile improvement initiative for mental health medicines

Sue Jordan / Timothy Banner / Marie Gabe-Walters / Jane M Mikhail / Gerwyn Panes / Jeff Round / Sherrill Snelgrove / Mel Storey / David Hughes / Medicines’ Management Group, Swansea University

PLoS ONE, Vol 14, Iss 9, p e

An observational and interview study.

2019 Volume 0220885

Abstract: Introduction Preventable adverse effects of medicines often pass unnoticed, but lead to real harm. Intervention Nurse-led monitoring using the structured Adverse Drug Reaction (ADRe) Profile identifies and addresses adverse effects of mental health ... ...

Abstract	Introduction Preventable adverse effects of medicines often pass unnoticed, but lead to real harm. Intervention Nurse-led monitoring using the structured Adverse Drug Reaction (ADRe) Profile identifies and addresses adverse effects of mental health medicines. Objectives This study investigated the implementation and clinical impact of ADRe, and barriers to and facilitators of sustained utilisation in routine practice. Methods Administration of ADRe was observed for 30 residents prescribed mental health medicines in ten care homes. The study pharmacist reviewed completed ADRes against medication records. Policy context was explored in 30 interviews with service users, nurse managers and strategic leads in Wales. Results Residents were aged 60-95, and prescribed 1-17 (median 9 [interquartile range (IQR) 7-13]) medicines. ADRe identified a median of 18 [IQR 11.5-23] problems per resident and nurses made 2 [1-2] changes to care per resident. For example: falls were reported for 9 residents, and care was modified for 5; pain was identified in 8 residents, and alleviated for 7; all 6 residents recognised as dyspnoeic were referred to prescribers. Nurses referred 17 of 30 residents to prescribers. Pharmacists recommended review for all 30. Doubts about administering ADRe, sometimes expressed by people who had not yet used it, diminished as it became familiar. ADRe was needed to bridge communication between resident, nurses and prescribers. When barriers of time, complacency, and doctors' non-availability were overcome, reporting with ADRe made prescribers more likely to heed nurses' concerns regarding residents' welfare. Clinical gains were facilitated by one-to-one time, staff-resident relationships, and unification of documentation. Implications To our knowledge, ADRe is the only instrument that brings a full account of patients' problems to medication reviews. This juxtaposition of signs and symptoms against prescriptions facilitates dose adjustments and de-prescribing and leads to: reduced pain and sedation; early identification of problems linked to ADRs, such as falls; and timely medication reviews e.g. for dyspnoea.
Keywords	Medicine ; R ; Science ; Q
Subject code	360
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Nitrogen-deficient microalgae are rich in cell-surface mannose: potential implications for prey biorecognition by phagotrophic protozoa

Martel, Claire M.(Swansea University School of Medicine Institute of Life Science)

Brazilian Journal of Microbiology

2009/03

Abstract: Flow cytometry was used to quantify the abundance of mannose-linked glycoconjugates on microalgae precultured using low- or high-nitrate media. Nitrogen-deficient microalgae were richer in cell-surface mannose than nitrogen-sufficient. Findings are ... ...

Abstract	Flow cytometry was used to quantify the abundance of mannose-linked glycoconjugates on microalgae precultured using low- or high-nitrate media. Nitrogen-deficient microalgae were richer in cell-surface mannose than nitrogen-sufficient. Findings are discussed in view of recent research which reveals mannose-specific 'feeding receptors' assist prey biorecognition by phagotrophic protozoa that ingest microalgae.
Language	English
Document type	Article
ISSN	1517-8382
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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Book ; Online: Emotional And Behavioural Difficulties In Middle Childhood

Maurice Chazan Emeritus Professor of Education, University College of Swansea / Alice F. Laing Honorary Research Fellow, Department of Education at University College Swansea / Diane Davies Deputy Headteacher at Tywyn Primary School, Port Talbot Wales

Identification, Assessment And Intervention In School

2014

Abstract: Looking In Depth At The Main Issues Of Emotional And Behavioural difficulties of 7-11 year olds, this book draws on recent study material and projects to suggest practical ways of dealing with such difficulties in schools, and to give a clearer ... ...

Abstract	Looking In Depth At The Main Issues Of Emotional And Behavioural difficulties of 7-11 year olds, this book draws on recent study material and projects to suggest practical ways of dealing with such difficulties in schools, and to give a clearer understanding of the problems posed by children with EBDs. Key topics covered include educating children with Emotional And Behavioural Difficulties Ebds In Mainstream And Special schools, disruptive behaviour and bullying, withdrawal, anxiety and depression, identification and assessment and how schools, parents and others can help
Language	English
Size	Online-Ressource (439 p)
Publisher	Taylor and Francis
Publishing place	Hoboken
Document type	Book ; Online
Note	Description based upon print version of record
ISBN	9780750703468 ; 0750703466
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Article: Genome Organization and Transcription Response to Harvest of Two Metallothionein-Like Genes in Agaricus bisporus Fruiting Bodies

Eastwood, Daniel C., University of Swansea, Sigleton Park, Swansea, Wales UK / Bains, Navdeep K., University of Warwick, Wellesbourne, Warwickshire, UK / Henderson, Janey, Coventry University, Priory Street, Coventry, UK / Burton, Kerry S., University of Warwick, Wellesbourne, Warwickshire, UK

Journal of Microbiology and Biotechnology

(May 2011) Volume v. 21, Issue (5), Page(s) p. 455–463

Abstract: Metallothioneins are a class of small cysteine-rich proteins that have been associated with increased tolerance to metal and oxidative stresses in animals, plants, and fungi. We investigated a metallothionein-like (mt-like) gene shown previously to be ... ...

Abstract	Metallothioneins are a class of small cysteine-rich proteins that have been associated with increased tolerance to metal and oxidative stresses in animals, plants, and fungi. We investigated a metallothionein-like (mt-like) gene shown previously to be upregulated in fruiting bodies of the fungus Agaricus bisporus in response to post-harvest storage. Analysis of an A. bisporus genomic DNA cosmid library identified two similar mt-like genes (met1 and met2) arranged as a bidirectional gene pair transcribed from the same promoter region. The promoter contained regulatory elements including 9 metal responsive elements and a CAAT box region 220 bp downstream of met1 that showed striking similarity to a feature in Coprinopsis cinerea mt-like gene promoters. Transcriptional analysis showed that both met genes are significantly and rapidly (within 3 hours) upregulated during post-harvest storage and expression is significantly greater in stipe and cap tissues compared with the gills. However, a strong directionality of the promoter was demonstrated, as transcript levels of met1 were at least two orders of magnitude greater than those of met2 in all samples tested.
Keywords	METALLOPROTEINS ; THIONEINS ; AGARICUS BISPORUS ; METALLOPROTEINE ; THIONEINE ; AGARICUS BISPORUS ; METALPROTEINAS ; TIONEINA ; AGARICUS BISPORUS
Language	English
Document type	Article
ISSN	1017-7825
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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Article: Use of different growth paramaters of Norway spruce (Picea abies (L.) Karst.) to study tree response to climate

Levanic, T., Slovenian Forestry Institute, Ljubljana (Slovenia) / Jalkanen, R., METLA Rovaniemi research Unit, Rovaniemi (Finland) / Gricar, J., Slovenian Forestry Institute, Ljubljana (Slovenia) / Gagen, M., Univ. of Swansea, Department of Geography, Swansea (United Kingdom) / Oven, P., Ljubljana Univ. (Slovenia). Biotechnical Fac., Wood Science and Technology Dept.

Zbornik gozdarstva in lesarstva

2006 , Issue no. 8

Abstract: In the paper, potential analysis of various growth parameters of Norway spruceare introduced at the macro and micro levels. Dendroecological measurements give information as to xylem growth ring widths, their density and content of stable isotopes in the ...

Abstract	In the paper, potential analysis of various growth parameters of Norway spruceare introduced at the macro and micro levels. Dendroecological measurements give information as to xylem growth ring widths, their density and content of stable isotopes in the xylem growth rings. Needle trace method of the terminal annual shoot allows retrospective view into needle density ofthe terminal annual shoot and consequently reconstruction of the several parameters linked to the needles and air pollution. Using pinning method, it is possible to follow intra-annual dynamics of the radial growth of trees at the cellular level and furthermore investigate the effect of climatic factorson cambial activity
Keywords	PICEA ABIES ; CLIMATIC FACTORS ; SITE FACTORS ; LEAVES ; AIR POLLUTION ; DENSITY ; XYLEM ; CAMBIUM ; GROWTH RINGS ; PICEA ABIES ; FACTEUR CLIMATIQUE ; FACTEUR LIE AU SITE ; FEUILLE ; POLLUTION ATMOSPHERIQUE ; DENSITE ; XYLEME ; CAMBIUM ; CERNE ; PICEA ABIES ; FACTORES CLIMATICOS ; CARACTERISTICAS DEL SITIO ; HOJAS ; POLUCION DEL AIRE ; DENSIDAD ; XILEMA ; CAMBIUM ; ANILLO DE CRECIMIENTO ; http://www.fao.org/aos/agrovoc#c_5852 ; http://www.fao.org/aos/agrovoc#c_29554 ; http://www.fao.org/aos/agrovoc#c_7087 ; http://www.fao.org/aos/agrovoc#c_4243 ; http://www.fao.org/aos/agrovoc#c_228 ; http://www.fao.org/aos/agrovoc#c_2186 ; http://www.fao.org/aos/agrovoc#c_8471 ; http://www.fao.org/aos/agrovoc#c_1225 ; http://www.fao.org/aos/agrovoc#c_3399
Language	English
Document type	Article
ISSN	0351-3114
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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Book ; Online: National/Regional Health Promoting Hospitals Network coordinators

National/Regional Health Promoting Hospitals Network Coordinators. Workshop (5th: 1999: Swansea, Wales, United Kingdom) / World Health Organization. Regional Office for Europe

report on the fifth workshop, Swansea, Wales, United Kingdom 21 April 1999

1999

Abstract: English only ... EURO; HQ ... EUR/ICP/DLVR 05 03 02 ...

Abstract	English only EURO; HQ EUR/ICP/DLVR 05 03 02 7 p. The Workshop took place in conjunction with the 7th International Conference on Health Promoting Hospitals. The meeting was divided into two parts. The first part was devoted to a presentation of progress reports on the development of the national/regional Health Promoting Hospitals networks. Each coordinator briefly presented the development of the network, focusing also on successes and failures. The second purpose of the Workshop was to initiate a discussion on internationally agreed indicators for the evaluation of Health Promoting Hospitals programmes and projects. A keynote presentation on international indicators for Health Promoting Hospitals was given. The succeeding discussion demonstrated that more time is necessary to find agreement on indicators for Health Promoting Hospitals. The meeting concluded with suggestions concerning the further working structure of the workshops of network coordinators
Keywords	Health promotion ; Hospitals ; Program evaluation ; International cooperation ; Congresses ; Europe ; Community Health and Primary Health Care ; trends
Language	English
Publisher	Copenhagen : WHO Regional Office for Europe
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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