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  1. Book ; Online ; E-Book: Yeast membrane transport

    Ramos, José / Sychrova, Hana / Kschischo, Maik

    (Advances in experimental medicine and biology ; 892)

    2016  

    Author's details edited by José Ramos, Hana Sychrová, Maik Kschischo
    Series title Advances in experimental medicine and biology ; 892
    Collection
    Keywords Yeasts / physiology ; Biological Transport ; Membrane Transport Proteins ; Life sciences ; Biotechnology ; Cell membranes
    Subject code 571.64
    Language English
    Size 1 Online-Ressource (viii, 379 Seiten), Illustrationen
    Publisher Springer
    Publishing place Cham
    Publishing country Switzerland
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT019051404
    ISBN 978-3-319-25304-6 ; 9783319253022 ; 3-319-25304-2 ; 3319253026
    DOI 10.1007/978-3-319-25304-6
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: Yeast Trk1 Potassium Transporter Gradually Changes Its Affinity in Response to Both External and Internal Signals.

    Masaryk, Jakub / Sychrová, Hana

    Journal of fungi (Basel, Switzerland)

    2022  Volume 8, Issue 5

    Abstract: Yeasts need a high intracellular concentration of potassium to grow. The main ... ...

    Abstract Yeasts need a high intracellular concentration of potassium to grow. The main K
    Language English
    Publishing date 2022-04-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof8050432
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Light-induced antifungal activity of nanoparticles with an encapsulated porphyrin photosensitizer.

    Kodedová, Marie / Liška, Vojtěch / Mosinger, Jiří / Sychrová, Hana

    Microbiological research

    2023  Volume 269, Page(s) 127303

    Abstract: The strong antifungal effect of sulfonated polystyrene nanoparticles (NPs) with an encapsulated tetraphenylporphyrin (TPP) photosensitizer is reported here. TPP is activated by visible light, resulting in the generation of singlet oxygen. Its antifungal ... ...

    Abstract The strong antifungal effect of sulfonated polystyrene nanoparticles (NPs) with an encapsulated tetraphenylporphyrin (TPP) photosensitizer is reported here. TPP is activated by visible light, resulting in the generation of singlet oxygen. Its antifungal action is potentiated in the presence of potassium iodide, yielding I
    MeSH term(s) Photosensitizing Agents/pharmacology ; Photosensitizing Agents/chemistry ; Photosensitizing Agents/therapeutic use ; Antifungal Agents/pharmacology ; Antifungal Agents/therapeutic use ; Potassium Iodide/chemistry ; Potassium Iodide/pharmacology ; Photochemotherapy ; Nanoparticles/chemistry ; Porphyrins/pharmacology ; Porphyrins/chemistry
    Chemical Substances Photosensitizing Agents ; Antifungal Agents ; Potassium Iodide (1C4QK22F9J) ; Porphyrins
    Language English
    Publishing date 2023-01-13
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1189614-0
    ISSN 1618-0623 ; 0944-5013
    ISSN (online) 1618-0623
    ISSN 0944-5013
    DOI 10.1016/j.micres.2023.127303
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The Hydrophilic C-terminus of Yeast Plasma-membrane Na

    Zimmermannová, Olga / Velázquez, Diego / Papoušková, Klára / Průša, Vojtěch / Radová, Viktorie / Falson, Pierre / Sychrová, Hana

    Journal of molecular biology

    2024  Volume 436, Issue 4, Page(s) 168443

    Abstract: Yeast plasma-membrane ... ...

    Abstract Yeast plasma-membrane Na
    MeSH term(s) Lithium/metabolism ; Protons ; Sodium/metabolism ; Sodium-Hydrogen Exchangers/genetics ; Sodium-Hydrogen Exchangers/chemistry ; Zygosaccharomyces/metabolism ; Fungal Proteins/chemistry ; Fungal Proteins/genetics ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/genetics ; Potassium/metabolism
    Chemical Substances Lithium (9FN79X2M3F) ; Protons ; Sodium (9NEZ333N27) ; Sodium-Hydrogen Exchangers ; SOD2 protein, Zygosaccharomyces rouxii ; Fungal Proteins ; NHA1 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins ; Potassium (RWP5GA015D)
    Language English
    Publishing date 2024-01-09
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80229-3
    ISSN 1089-8638 ; 0022-2836
    ISSN (online) 1089-8638
    ISSN 0022-2836
    DOI 10.1016/j.jmb.2024.168443
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  5. Article: The Hrk1 kinase is a determinant of acetic acid tolerance in yeast by modulating H

    Antunes, Miguel / Kale, Deepika / Sychrová, Hana / Sá-Correia, Isabel

    Microbial cell (Graz, Austria)

    2023  Volume 10, Issue 12, Page(s) 261–276

    Abstract: Acetic acid-induced stress is a common challenge in natural environments and industrial bioprocesses, significantly affecting the growth and metabolic performance ... ...

    Abstract Acetic acid-induced stress is a common challenge in natural environments and industrial bioprocesses, significantly affecting the growth and metabolic performance of
    Language English
    Publishing date 2023-11-14
    Publishing country Austria
    Document type Journal Article
    ZDB-ID 2814756-X
    ISSN 2311-2638
    ISSN 2311-2638
    DOI 10.15698/mic2023.12.809
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Heterologous expression reveals unique properties of Trk K+ importers from nonconventional biotechnologically relevant yeast species together with their potential to support Saccharomyces cerevisiae growth

    Papoušková, Klára / Gómez, Marcos / Kodedová, Marie / Ramos, José / Zimmermannová, Olga / Sychrová, Hana

    Yeast. 2023 Feb., v. 40, no. 2 p.68-83

    2023  

    Abstract: In the model yeast Saccharomyces cerevisiae, Trk1 is the main K⁺ importer. It is involved in many important physiological processes, such as the maintenance of ion homeostasis, cell volume, intracellular pH, and plasma‐membrane potential. The ScTrk1 ... ...

    Abstract In the model yeast Saccharomyces cerevisiae, Trk1 is the main K⁺ importer. It is involved in many important physiological processes, such as the maintenance of ion homeostasis, cell volume, intracellular pH, and plasma‐membrane potential. The ScTrk1 protein can be of great interest to industry, as it was shown that changes in its activity influence ethanol production and tolerance in S. cerevisiae and also cell performance in the presence of organic acids or high ammonium under low K⁺ conditions. Nonconventional yeast species are attracting attention due to their unique properties and as a potential source of genes that encode proteins with unusual characteristics. In this work, we aimed to study and compare Trk proteins from Debaryomyces hansenii, Hortaea werneckii, Kluyveromyces marxianus, and Yarrowia lipolytica, four biotechnologically relevant yeasts that tolerate various extreme environments. Heterologous expression in S. cerevisiae cells lacking the endogenous Trk importers revealed differences in the studied Trk proteins' abilities to support the growth of cells under various cultivation conditions such as low K⁺ or the presence of toxic cations, to reduce plasma‐membrane potential or to take up Rb⁺. Examination of the potential of Trks to support the stress resistance of S. cerevisiae wild‐type strains showed that Y. lipolytica Trk1 is a promising tool for improving cell tolerance to both low K⁺ and high salt and that the overproduction of S. cerevisiae's own Trk1 was the most efficient at improving the growth of cells in the presence of highly toxic Li⁺ ions.
    Keywords Debaryomyces hansenii ; Kluyveromyces marxianus ; Saccharomyces cerevisiae ; Yarrowia lipolytica ; ammonium ; ethanol production ; heterologous gene expression ; homeostasis ; industry ; pH ; plasma membrane ; stress tolerance ; toxicity ; yeasts
    Language English
    Dates of publication 2023-02
    Size p. 68-83.
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 632636-5
    ISSN 1097-0061 ; 0749-503X
    ISSN (online) 1097-0061
    ISSN 0749-503X
    DOI 10.1002/yea.3834
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4410: Show issues

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  7. Article: Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration

    Dauss, Edgars / Papoušková, Klára / Sychrová, Hana / Rapoport, Alexander

    Antonie van Leeuwenhoek. 2021 July, v. 114, no. 7

    2021  

    Abstract: Two Saccharomyces cerevisiae strains, BY4741 and BY4741-derived strain lacking the IST2 gene (ist2Δ), were used to characterise the possible role of cortical endoplasmic reticulum (ER) protein Ist2 upon cell dehydration and subsequent rehydration. For ... ...

    Abstract Two Saccharomyces cerevisiae strains, BY4741 and BY4741-derived strain lacking the IST2 gene (ist2Δ), were used to characterise the possible role of cortical endoplasmic reticulum (ER) protein Ist2 upon cell dehydration and subsequent rehydration. For the first time, we show that not only protein components of the plasma membrane (PM), but also at least one ER membrane protein (Ist2) play an important role in the maintenance of the viability of yeast cells during dehydration and subsequent rehydration. The low viability of the mutant strain ist2∆ upon dehydration-rehydration stress was related to the lack of Ist2 protein in the ER. We revealed that the PM of ist2∆ strain is not able to completely restore its molecular organisation during reactivation from the state of anhydrobiosis. As the result, the permeability of the PM remains high regardless of the type of reactivation (rapid or gradual rehydration). We conclude that ER protein Ist2 plays an important role in ensuring the stability of molecular organisation and functionality of the PM during dehydration-rehydration stress. These results indicate an important role of ER-PM interactions during cells transition into the state of anhydrobiosis and the subsequent restoration of their physiological activities.
    Keywords Saccharomyces cerevisiae ; anhydrobiosis ; endoplasmic reticulum ; genes ; membrane proteins ; mutants ; permeability ; plasma membrane ; rehydration ; viability ; yeasts
    Language English
    Dates of publication 2021-07
    Size p. 1069-1077.
    Publishing place Springer International Publishing
    Document type Article
    ZDB-ID 214861-4
    ISSN 1572-9699 ; 0003-6072
    ISSN (online) 1572-9699
    ISSN 0003-6072
    DOI 10.1007/s10482-021-01578-8
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  8. Article ; Online: Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration.

    Dauss, Edgars / Papoušková, Klára / Sychrová, Hana / Rapoport, Alexander

    Antonie van Leeuwenhoek

    2021  Volume 114, Issue 7, Page(s) 1069–1077

    Abstract: Two Saccharomyces cerevisiae strains, BY4741 and BY4741-derived strain lacking the IST2 gene (ist2Δ), were used to characterise the possible role of cortical endoplasmic reticulum (ER) protein Ist2 upon cell dehydration and subsequent rehydration. For ... ...

    Abstract Two Saccharomyces cerevisiae strains, BY4741 and BY4741-derived strain lacking the IST2 gene (ist2Δ), were used to characterise the possible role of cortical endoplasmic reticulum (ER) protein Ist2 upon cell dehydration and subsequent rehydration. For the first time, we show that not only protein components of the plasma membrane (PM), but also at least one ER membrane protein (Ist2) play an important role in the maintenance of the viability of yeast cells during dehydration and subsequent rehydration. The low viability of the mutant strain ist2∆ upon dehydration-rehydration stress was related to the lack of Ist2 protein in the ER. We revealed that the PM of ist2∆ strain is not able to completely restore its molecular organisation during reactivation from the state of anhydrobiosis. As the result, the permeability of the PM remains high regardless of the type of reactivation (rapid or gradual rehydration). We conclude that ER protein Ist2 plays an important role in ensuring the stability of molecular organisation and functionality of the PM during dehydration-rehydration stress. These results indicate an important role of ER-PM interactions during cells transition into the state of anhydrobiosis and the subsequent restoration of their physiological activities.
    MeSH term(s) Endoplasmic Reticulum ; Fluid Therapy ; Humans ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/genetics
    Chemical Substances IST2 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins
    Language English
    Publishing date 2021-04-12
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 214861-4
    ISSN 1572-9699 ; 0003-6072
    ISSN (online) 1572-9699
    ISSN 0003-6072
    DOI 10.1007/s10482-021-01578-8
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  9. Article ; Online: The second intracellular loop of the yeast Trk1 potassium transporter is involved in regulation of activity, and interaction with 14-3-3 proteins

    Masaryk, Jakub / Kale, Deepika / Pohl, Pavel / Ruiz-Castilla, Francisco J. / Zimmermannová, Olga / Obšilová, Veronika / Ramos, José / Sychrová, Hana

    Computational and Structural Biotechnology Journal. 2023 Apr. 20,

    2023  

    Abstract: Potassium is an essential intracellular ion, and a sufficient intracellular concentration of it is crucial for many processes; therefore it is fundamental for cells to precisely regulate K⁺ uptake and efflux through the plasma membrane. The uniporter ... ...

    Abstract Potassium is an essential intracellular ion, and a sufficient intracellular concentration of it is crucial for many processes; therefore it is fundamental for cells to precisely regulate K⁺ uptake and efflux through the plasma membrane. The uniporter Trk1 is a key player in K⁺ acquisition in yeasts. The TRK1 gene is expressed at a low and stable level; thus the activity of the transporter needs to be regulated at a posttranslational level. S. cerevisiae Trk1 changes its activity and affinity for potassium ion quickly and according to both internal and external concentrations of K⁺, as well as the membrane potential. The molecular basis of these changes has not been elucidated, though phosphorylation is thought to play an important role. In this study, we examined the role of the second, short, and highly conserved intracellular hydrophilic loop of Trk1 (IL2), and identified two phosphorylable residues (Ser882 and Thr 900) as very important for 1) the structure of the loop and consequently for the targeting of Trk1 to the plasma membrane, and 2) the upregulation of the transporter's activity reaching maximal affinity under low external K⁺ conditions. Moreover, we identified three residues (Thr155, Ser414, and Thr900) within the Trk1 protein as strong candidates for interaction with 14-3-3 regulatory proteins, and showed, in an in vitro experiment, that phosphorylated Thr900 of the IL2 indeed binds to both isoforms of yeast 14-3-3 proteins, Bmh1 and Bmh2.
    Keywords biotechnology ; genes ; hydrophilicity ; interleukin-2 ; membrane potential ; phosphorylation ; plasma membrane ; potassium ; yeasts ; potassium ion uptake ; Saccharomyces cerevisiae ; Trk1 ; 14-3-3 proteins
    Language English
    Dates of publication 2023-0420
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note Pre-press version ; Use and reproduction
    ZDB-ID 2694435-2
    ISSN 2001-0370
    ISSN 2001-0370
    DOI 10.1016/j.csbj.2023.04.019
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Styrylpyridinium Derivatives as New Potent Antifungal Drugs and Fluorescence Probes.

    Vaitkienė, Simona / Daugelavičius, Rimantas / Sychrová, Hana / Kodedová, Marie

    Frontiers in microbiology

    2020  Volume 11, Page(s) 2077

    Abstract: The incidence ... ...

    Abstract The incidence of
    Language English
    Publishing date 2020-08-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.02077
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