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  1. Article ; Online: Applications and prospects of 3D printing technology in pulp regeneration

    TAN Guozhong / JIANG Qianzhou

    口腔疾病防治, Vol 29, Iss 4, Pp 279-

    2021  Volume 283

    Abstract: In recent years, pulp regeneration has become a research hotspot in the field of stomatology. 3D printing can realize precise control of structure and shape of scaffolds, which provide basis for seed cell adhesion and growth factor release. The 3D ... ...

    Abstract In recent years, pulp regeneration has become a research hotspot in the field of stomatology. 3D printing can realize precise control of structure and shape of scaffolds, which provide basis for seed cell adhesion and growth factor release. The 3D printing "pulp complexes" constructed by 3D printing scaffolds for tissue engineering provides a new direction for pulp regeneration research. This paper reviews the applications of 3D printing technology in pulp regeneration. The results of literature review showed that the scaffold materials, seed cells and growth factors in the 3D printing "pulp complexes" all play an important role in the pulp regeneration research. Among them, the scaffold materials act as carriers to load seed cells and growth factors and provide a suitable microenvironment for them. The common seed cells such as dental pulp stem cells, stem cells from apical papilla and stem cells from the human pulp of exfoliated deciduous teeth can provide the cellular basis for pulp regeneration. Moreover, the introduction of growth factors can further support the differentiation of pulp tissue and the reconstruction of pulp vessels and promote pulp regeneration. At present, the 3D printing "pulp complexes" in the study of dental pulp regeneration has made some progress and can induce the formation of pulp-like tissues in the laboratory. However, preparing 3D-printing "pulp complex" with good biological activity, which integrates biomimetic blood vessels and nerves to supply oxygen and nutrients to the cells in the root canal, remains a huge challenge and still needs further exploration and research.
    Keywords pulp regeneration ; tissue engineering ; 3d printing ; pulp complexes ; scaffold materials ; seed cells ; growth factors ; pulp vascular reconstruction ; Medicine ; R
    Subject code 571
    Language Chinese
    Publishing date 2021-04-01T00:00:00Z
    Publisher Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Research on the osteogenesis and biosafety of ECM-Loaded 3D-Printed Gel/SA/58sBG scaffolds.

    Tan, Guozhong / Chen, Rongfeng / Tu, Xinran / Guo, Liyang / Guo, Lvhua / Xu, Jingyi / Zhang, Chengfei / Zou, Ting / Sun, Shuyu / Jiang, Qianzhou

    Frontiers in bioengineering and biotechnology

    2022  Volume 10, Page(s) 973886

    Abstract: Employing scaffolds containing cell-derived extracellular matrix (ECM) as an alternative strategy for the regeneration of bone defects has shown prominent advantages. Here, gelatin (Gel), sodium alginate (SA) and 58s bioactive glass (58sBG) were ... ...

    Abstract Employing scaffolds containing cell-derived extracellular matrix (ECM) as an alternative strategy for the regeneration of bone defects has shown prominent advantages. Here, gelatin (Gel), sodium alginate (SA) and 58s bioactive glass (58sBG) were incorporated into deionized water to form ink, which was further fabricated into composite scaffolds by the 3D printing technique. Then, rat aortic endothelial cells (RAOECs) or rat bone mesenchymal stem cells (RBMSCs) were seeded on the scaffolds. After decellularization, two kinds of ECM-loaded scaffolds (RAOECs-ECM scaffold and RBMSCs-ECM scaffold) were obtained. The morphological characteristics of the scaffolds were assessed meticulously by scanning electron microscopy (SEM). In addition, the effects of scaffolds on the proliferation, adhesion, and osteogenic and angiogenic differentiation of RBMSCs were evaluated by Calcein AM staining and reverse transcription polymerase chain reaction (RT-PCR).
    Language English
    Publishing date 2022-08-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2022.973886
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: 3D-printed gelatin/sodium alginate/58S bioactive glass scaffolds promote osteogenesis

    Tu, Xinran / Guo, Liyang / Li, Yang / Tan, Guozhong / Chen, Rongfeng / Wu, Jingwen / Miao, Guohou / Guo, Lvhua / Zhang, Chengfei / Zou, Ting / Zhang, Yang / Jiang, Qianzhou

    Journal of biomaterials applications

    2023  Volume 37, Issue 10, Page(s) 1758–1766

    Abstract: Three-dimensional (3D)-printed scaffolds are a new strategy to fabricate biomaterials for treating bone defects. Here, using a 3D-printing technique, we fabricated scaffolds consisting of gelatin (Gel), sodium alginate (SA), and 58S bioactive glass (58S ... ...

    Abstract Three-dimensional (3D)-printed scaffolds are a new strategy to fabricate biomaterials for treating bone defects. Here, using a 3D-printing technique, we fabricated scaffolds consisting of gelatin (Gel), sodium alginate (SA), and 58S bioactive glass (58S BG). To evaluate mechanical properties and biocompatibility of Gel/SA/58S BG scaffolds, the degradation test, compressive strength test, and cytotoxicity test were performed. The effect of the scaffolds on cell proliferation in vitro was determined by 4',6-diamidino-2-phenylindole (DAPI) staining. To evaluate osteoinductive properties, rBMSCs were cultured on the scaffolds for 7, 14, and 21 days and the expression of osteogenesis-related genes was analyzed using qRT-PCR. To examine the bone healing properties of Gel/SA/58S BG scaffolds in vivo, we used a rat mandibular critical-size defect bone model. The scaffolds were implanted into the defect area of rat mandible and bone regeneration and new tissue formation were assessed using microcomputed tomography (microCT) and hematoxylin and eosin (H&E) staining. The results showed that Gel/SA/58S BG scaffolds had appropriate mechanical strength as a filling material for bone defects. Furthermore, the scaffolds could be compressed within certain limits and then could recover their shape. The extract of the Gel/SA/58S BG scaffold showed no cytotoxicity. In vitro, the expression levels of
    MeSH term(s) Rats ; Animals ; Osteogenesis ; Tissue Scaffolds ; Gelatin ; Alginates ; X-Ray Microtomography ; Biocompatible Materials ; Bone Regeneration ; Glass ; Printing, Three-Dimensional ; Tissue Engineering/methods
    Chemical Substances bioactive glass 58S ; Gelatin (9000-70-8) ; Alginates ; Biocompatible Materials
    Language English
    Publishing date 2023-03-27
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639283-0
    ISSN 1530-8022 ; 0885-3282
    ISSN (online) 1530-8022
    ISSN 0885-3282
    DOI 10.1177/08853282231152128
    Database MEDical Literature Analysis and Retrieval System OnLINE

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